Experiment: M9+glycerol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HGI48_RS12650 and HGI48_RS12655 are separated by 173 nucleotides HGI48_RS12655 and HGI48_RS12660 are separated by 262 nucleotides HGI48_RS12660 and HGI48_RS12665 are separated by 130 nucleotides HGI48_RS12665 and HGI48_RS12670 are separated by 109 nucleotides
HGI48_RS12650: HGI48_RS12650 - transcriptional regulator SlyA, at 2,766,761 to 2,767,198
_RS12650
HGI48_RS12655: HGI48_RS12655 - DUF1289 domain-containing protein, at 2,767,372 to 2,767,611
_RS12655
HGI48_RS12660: HGI48_RS12660 - lactoylglutathione lyase, at 2,767,874 to 2,768,281
_RS12660
HGI48_RS12665: HGI48_RS12665 - ribonuclease T, at 2,768,412 to 2,769,107
_RS12665
HGI48_RS12670: HGI48_RS12670 - Grx4 family monothiol glutaredoxin, at 2,769,217 to 2,769,567
_RS12670
Position (kb)
2767
2768
2769 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2766.881 kb on - strand, within HGI48_RS12650 at 2766.905 kb on - strand, within HGI48_RS12650 at 2766.931 kb on + strand, within HGI48_RS12650 at 2766.932 kb on - strand, within HGI48_RS12650 at 2766.932 kb on - strand, within HGI48_RS12650 at 2766.932 kb on - strand, within HGI48_RS12650 at 2766.971 kb on - strand, within HGI48_RS12650 at 2766.974 kb on + strand, within HGI48_RS12650 at 2766.974 kb on + strand, within HGI48_RS12650 at 2766.974 kb on + strand, within HGI48_RS12650 at 2766.975 kb on - strand, within HGI48_RS12650 at 2766.975 kb on - strand, within HGI48_RS12650 at 2766.975 kb on - strand, within HGI48_RS12650 at 2767.003 kb on + strand, within HGI48_RS12650 at 2767.003 kb on + strand, within HGI48_RS12650 at 2767.003 kb on + strand, within HGI48_RS12650 at 2767.003 kb on + strand, within HGI48_RS12650 at 2767.003 kb on + strand, within HGI48_RS12650 at 2767.003 kb on + strand, within HGI48_RS12650 at 2767.004 kb on - strand, within HGI48_RS12650 at 2767.004 kb on - strand, within HGI48_RS12650 at 2767.004 kb on - strand, within HGI48_RS12650 at 2767.004 kb on - strand, within HGI48_RS12650 at 2767.081 kb on + strand, within HGI48_RS12650 at 2767.081 kb on + strand, within HGI48_RS12650 at 2767.081 kb on + strand at 2767.081 kb on + strand, within HGI48_RS12650 at 2767.081 kb on + strand, within HGI48_RS12650 at 2767.081 kb on + strand, within HGI48_RS12650 at 2767.082 kb on - strand at 2767.082 kb on - strand, within HGI48_RS12650 at 2767.084 kb on + strand, within HGI48_RS12650 at 2767.084 kb on + strand, within HGI48_RS12650 at 2767.084 kb on + strand, within HGI48_RS12650 at 2767.085 kb on - strand, within HGI48_RS12650 at 2767.154 kb on + strand, within HGI48_RS12650 at 2767.154 kb on + strand, within HGI48_RS12650 at 2767.154 kb on + strand, within HGI48_RS12650 at 2767.172 kb on + strand at 2767.184 kb on + strand at 2767.220 kb on + strand at 2767.223 kb on + strand at 2767.269 kb on + strand at 2767.269 kb on + strand at 2767.269 kb on + strand at 2767.286 kb on + strand at 2767.287 kb on - strand at 2767.287 kb on - strand at 2767.300 kb on + strand at 2767.300 kb on + strand at 2767.300 kb on + strand at 2767.300 kb on + strand at 2767.313 kb on - strand at 2767.319 kb on + strand at 2767.319 kb on + strand at 2767.346 kb on + strand at 2767.347 kb on - strand at 2767.347 kb on - strand at 2767.354 kb on + strand at 2767.462 kb on + strand, within HGI48_RS12655 at 2767.463 kb on - strand at 2767.686 kb on + strand at 2767.686 kb on + strand at 2767.686 kb on + strand at 2767.687 kb on - strand at 2767.687 kb on - strand at 2767.729 kb on - strand at 2767.729 kb on - strand at 2767.786 kb on + strand at 2767.786 kb on + strand at 2767.788 kb on + strand at 2767.788 kb on + strand at 2767.789 kb on - strand at 2767.881 kb on + strand at 2767.890 kb on - strand at 2767.932 kb on - strand, within HGI48_RS12660 at 2767.988 kb on + strand, within HGI48_RS12660 at 2767.989 kb on - strand, within HGI48_RS12660 at 2768.012 kb on - strand, within HGI48_RS12660 at 2768.137 kb on + strand, within HGI48_RS12660 at 2768.138 kb on - strand, within HGI48_RS12660 at 2768.161 kb on + strand, within HGI48_RS12660 at 2768.161 kb on + strand, within HGI48_RS12660 at 2768.162 kb on - strand, within HGI48_RS12660 at 2768.463 kb on + strand at 2768.464 kb on - strand at 2768.464 kb on - strand at 2768.688 kb on + strand, within HGI48_RS12665 at 2768.732 kb on + strand, within HGI48_RS12665 at 2768.965 kb on - strand, within HGI48_RS12665 at 2769.105 kb on + strand at 2769.106 kb on - strand at 2769.205 kb on + strand at 2769.206 kb on - strand at 2769.219 kb on - strand at 2769.219 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9+glycerol remove 2,766,881 - HGI48_RS12650 0.27 +3.4 2,766,905 - HGI48_RS12650 0.33 +3.1 2,766,931 + HGI48_RS12650 0.39 +1.0 2,766,932 - HGI48_RS12650 0.39 +2.3 2,766,932 - HGI48_RS12650 0.39 +3.1 2,766,932 - HGI48_RS12650 0.39 +2.5 2,766,971 - HGI48_RS12650 0.48 +3.1 2,766,974 + HGI48_RS12650 0.49 +2.2 2,766,974 + HGI48_RS12650 0.49 +2.5 2,766,974 + HGI48_RS12650 0.49 +2.8 2,766,975 - HGI48_RS12650 0.49 -0.5 2,766,975 - HGI48_RS12650 0.49 +1.4 2,766,975 - HGI48_RS12650 0.49 +4.7 2,767,003 + HGI48_RS12650 0.55 +2.5 2,767,003 + HGI48_RS12650 0.55 +3.4 2,767,003 + HGI48_RS12650 0.55 +2.9 2,767,003 + HGI48_RS12650 0.55 +2.7 2,767,003 + HGI48_RS12650 0.55 +1.9 2,767,003 + HGI48_RS12650 0.55 +2.9 2,767,004 - HGI48_RS12650 0.55 +3.1 2,767,004 - HGI48_RS12650 0.55 +4.0 2,767,004 - HGI48_RS12650 0.55 +3.6 2,767,004 - HGI48_RS12650 0.55 +3.6 2,767,081 + HGI48_RS12650 0.73 +2.8 2,767,081 + HGI48_RS12650 0.73 +3.2 2,767,081 + -1.3 2,767,081 + HGI48_RS12650 0.73 +3.8 2,767,081 + HGI48_RS12650 0.73 +1.1 2,767,081 + HGI48_RS12650 0.73 +3.2 2,767,082 - -1.3 2,767,082 - HGI48_RS12650 0.73 +2.2 2,767,084 + HGI48_RS12650 0.74 +2.7 2,767,084 + HGI48_RS12650 0.74 +1.7 2,767,084 + HGI48_RS12650 0.74 +2.4 2,767,085 - HGI48_RS12650 0.74 +3.0 2,767,154 + HGI48_RS12650 0.90 +3.5 2,767,154 + HGI48_RS12650 0.90 +3.2 2,767,154 + HGI48_RS12650 0.90 +1.4 2,767,172 + +4.3 2,767,184 + +3.5 2,767,220 + +2.0 2,767,223 + +3.2 2,767,269 + +2.1 2,767,269 + -2.3 2,767,269 + +4.0 2,767,286 + +2.8 2,767,287 - +1.9 2,767,287 - +2.8 2,767,300 + +1.9 2,767,300 + +3.1 2,767,300 + +3.1 2,767,300 + +3.0 2,767,313 - -2.0 2,767,319 + +2.2 2,767,319 + +2.4 2,767,346 + +1.6 2,767,347 - +0.2 2,767,347 - +0.5 2,767,354 + +2.7 2,767,462 + HGI48_RS12655 0.38 +2.3 2,767,463 - +2.5 2,767,686 + -2.0 2,767,686 + +0.6 2,767,686 + -0.5 2,767,687 - -1.5 2,767,687 - +0.1 2,767,729 - -2.0 2,767,729 - +0.7 2,767,786 + -0.9 2,767,786 + -0.1 2,767,788 + -2.5 2,767,788 + +1.1 2,767,789 - -1.3 2,767,881 + +0.2 2,767,890 - -1.0 2,767,932 - HGI48_RS12660 0.14 -1.1 2,767,988 + HGI48_RS12660 0.28 -2.3 2,767,989 - HGI48_RS12660 0.28 -3.3 2,768,012 - HGI48_RS12660 0.34 -2.0 2,768,137 + HGI48_RS12660 0.64 -0.4 2,768,138 - HGI48_RS12660 0.65 -2.9 2,768,161 + HGI48_RS12660 0.70 -0.2 2,768,161 + HGI48_RS12660 0.70 -0.5 2,768,162 - HGI48_RS12660 0.71 -0.7 2,768,463 + -1.1 2,768,464 - +0.5 2,768,464 - -2.0 2,768,688 + HGI48_RS12665 0.40 -0.4 2,768,732 + HGI48_RS12665 0.46 -0.0 2,768,965 - HGI48_RS12665 0.79 +0.7 2,769,105 + +3.1 2,769,106 - -0.7 2,769,205 + +0.6 2,769,206 - -1.7 2,769,219 - -0.3 2,769,219 - -0.3
Or see this region's nucleotide sequence