Experiment: M9+glycerol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HGI48_RS03010 and HGI48_RS03015 overlap by 4 nucleotides HGI48_RS03015 and HGI48_RS03020 are separated by 77 nucleotides HGI48_RS03020 and HGI48_RS03025 overlap by 8 nucleotides HGI48_RS03025 and HGI48_RS03030 are separated by 124 nucleotides HGI48_RS03030 and HGI48_RS03035 overlap by 4 nucleotides
HGI48_RS03010: HGI48_RS03010 - glycoside hydrolase family 104 protein, at 644,491 to 644,982
_RS03010
HGI48_RS03015: HGI48_RS03015 - LysB family phage lysis regulatory protein, at 644,979 to 645,428
_RS03015
HGI48_RS03020: HGI48_RS03020 - phage tail protein, at 645,506 to 645,937
_RS03020
HGI48_RS03025: HGI48_RS03025 - phage virion morphogenesis protein, at 645,930 to 646,373
_RS03025
HGI48_RS03030: HGI48_RS03030 - phage baseplate assembly protein V, at 646,498 to 647,031
_RS03030
HGI48_RS03035: HGI48_RS03035 - GPW/gp25 family protein, at 647,028 to 647,378
_RS03035
Position (kb)
645
646
647 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 644.953 kb on + strand at 644.953 kb on + strand at 644.954 kb on - strand at 645.016 kb on + strand at 645.016 kb on + strand at 645.016 kb on + strand at 645.016 kb on + strand at 645.016 kb on + strand at 645.017 kb on - strand at 645.017 kb on - strand at 645.017 kb on - strand at 645.305 kb on + strand, within HGI48_RS03015 at 645.305 kb on + strand, within HGI48_RS03015 at 645.305 kb on + strand, within HGI48_RS03015 at 645.305 kb on + strand at 645.305 kb on + strand, within HGI48_RS03015 at 645.305 kb on + strand at 645.305 kb on + strand at 645.305 kb on + strand, within HGI48_RS03015 at 645.305 kb on + strand, within HGI48_RS03015 at 645.306 kb on - strand, within HGI48_RS03015 at 645.306 kb on - strand, within HGI48_RS03015 at 645.306 kb on - strand at 645.306 kb on - strand, within HGI48_RS03015 at 645.306 kb on - strand at 645.309 kb on - strand, within HGI48_RS03015 at 645.487 kb on - strand at 645.487 kb on - strand at 645.523 kb on - strand at 645.525 kb on + strand at 645.525 kb on + strand at 645.526 kb on - strand at 645.537 kb on + strand at 645.538 kb on - strand at 645.538 kb on - strand at 645.541 kb on + strand at 645.542 kb on - strand at 645.542 kb on - strand at 645.554 kb on + strand, within HGI48_RS03020 at 645.555 kb on - strand, within HGI48_RS03020 at 645.565 kb on + strand, within HGI48_RS03020 at 645.565 kb on + strand, within HGI48_RS03020 at 645.641 kb on + strand, within HGI48_RS03020 at 645.641 kb on + strand at 645.648 kb on - strand, within HGI48_RS03020 at 645.648 kb on - strand, within HGI48_RS03020 at 645.674 kb on + strand, within HGI48_RS03020 at 645.674 kb on + strand at 645.675 kb on - strand, within HGI48_RS03020 at 645.694 kb on - strand at 645.729 kb on - strand, within HGI48_RS03020 at 645.915 kb on - strand at 645.921 kb on + strand at 645.921 kb on + strand at 645.921 kb on + strand at 645.922 kb on - strand at 645.922 kb on - strand at 645.922 kb on - strand at 645.998 kb on + strand, within HGI48_RS03025 at 645.999 kb on - strand, within HGI48_RS03025 at 645.999 kb on - strand, within HGI48_RS03025 at 645.999 kb on - strand, within HGI48_RS03025 at 645.999 kb on - strand, within HGI48_RS03025 at 646.018 kb on + strand at 646.030 kb on + strand, within HGI48_RS03025 at 646.030 kb on + strand at 646.031 kb on - strand, within HGI48_RS03025 at 646.115 kb on + strand, within HGI48_RS03025 at 646.115 kb on + strand, within HGI48_RS03025 at 646.153 kb on + strand, within HGI48_RS03025 at 646.153 kb on + strand, within HGI48_RS03025 at 646.153 kb on + strand at 646.153 kb on + strand, within HGI48_RS03025 at 646.153 kb on + strand, within HGI48_RS03025 at 646.153 kb on + strand, within HGI48_RS03025 at 646.154 kb on - strand, within HGI48_RS03025 at 646.154 kb on - strand, within HGI48_RS03025 at 646.154 kb on - strand, within HGI48_RS03025 at 646.154 kb on - strand, within HGI48_RS03025 at 646.154 kb on - strand, within HGI48_RS03025 at 646.167 kb on + strand, within HGI48_RS03025 at 646.293 kb on + strand at 646.294 kb on - strand, within HGI48_RS03025 at 646.294 kb on - strand, within HGI48_RS03025 at 646.395 kb on + strand at 646.395 kb on + strand at 646.395 kb on + strand at 646.395 kb on + strand at 646.395 kb on + strand at 646.396 kb on - strand at 646.419 kb on + strand at 646.435 kb on + strand at 646.435 kb on + strand at 646.435 kb on + strand at 646.447 kb on + strand at 646.476 kb on + strand at 646.477 kb on - strand at 646.611 kb on - strand at 646.704 kb on + strand, within HGI48_RS03030 at 646.780 kb on - strand, within HGI48_RS03030 at 646.825 kb on + strand, within HGI48_RS03030 at 646.825 kb on + strand, within HGI48_RS03030 at 646.957 kb on - strand, within HGI48_RS03030 at 646.970 kb on + strand, within HGI48_RS03030 at 646.970 kb on + strand, within HGI48_RS03030 at 646.970 kb on + strand, within HGI48_RS03030 at 646.970 kb on + strand, within HGI48_RS03030 at 646.971 kb on - strand, within HGI48_RS03030 at 646.971 kb on - strand, within HGI48_RS03030 at 646.971 kb on - strand, within HGI48_RS03030 at 646.971 kb on - strand, within HGI48_RS03030 at 646.971 kb on - strand at 646.971 kb on - strand, within HGI48_RS03030 at 646.971 kb on - strand, within HGI48_RS03030 at 646.974 kb on + strand, within HGI48_RS03030 at 646.974 kb on + strand, within HGI48_RS03030 at 646.974 kb on + strand, within HGI48_RS03030 at 646.975 kb on - strand, within HGI48_RS03030 at 646.975 kb on - strand, within HGI48_RS03030 at 646.994 kb on + strand at 646.995 kb on - strand at 647.011 kb on - strand at 647.011 kb on - strand at 647.011 kb on - strand at 647.038 kb on - strand at 647.038 kb on - strand at 647.226 kb on + strand, within HGI48_RS03035 at 647.226 kb on + strand, within HGI48_RS03035 at 647.226 kb on + strand, within HGI48_RS03035 at 647.226 kb on + strand, within HGI48_RS03035 at 647.226 kb on + strand, within HGI48_RS03035 at 647.226 kb on + strand at 647.226 kb on + strand, within HGI48_RS03035 at 647.227 kb on - strand, within HGI48_RS03035 at 647.227 kb on - strand, within HGI48_RS03035 at 647.227 kb on - strand, within HGI48_RS03035 at 647.227 kb on - strand, within HGI48_RS03035
Per-strain Table
Position Strand Gene LocusTag Fraction M9+glycerol remove 644,953 + -0.9 644,953 + -0.1 644,954 - -1.0 645,016 + -0.9 645,016 + +2.0 645,016 + -0.9 645,016 + +0.5 645,016 + -2.6 645,017 - -1.9 645,017 - +0.1 645,017 - -2.7 645,305 + HGI48_RS03015 0.72 -2.7 645,305 + HGI48_RS03015 0.72 -1.6 645,305 + HGI48_RS03015 0.72 -0.5 645,305 + +3.6 645,305 + HGI48_RS03015 0.72 -1.9 645,305 + -0.9 645,305 + +0.7 645,305 + HGI48_RS03015 0.72 -1.9 645,305 + HGI48_RS03015 0.72 +0.7 645,306 - HGI48_RS03015 0.73 +0.4 645,306 - HGI48_RS03015 0.73 -0.7 645,306 - -0.9 645,306 - HGI48_RS03015 0.73 -2.6 645,306 - -0.9 645,309 - HGI48_RS03015 0.73 -3.2 645,487 - -1.9 645,487 - -0.9 645,523 - +0.7 645,525 + -0.4 645,525 + +1.2 645,526 - -0.3 645,537 + -0.9 645,538 - -0.3 645,538 - -1.3 645,541 + +1.4 645,542 - +1.1 645,542 - +3.4 645,554 + HGI48_RS03020 0.11 -1.1 645,555 - HGI48_RS03020 0.11 -1.5 645,565 + HGI48_RS03020 0.14 +1.0 645,565 + HGI48_RS03020 0.14 +1.0 645,641 + HGI48_RS03020 0.31 -1.6 645,641 + -0.9 645,648 - HGI48_RS03020 0.33 +0.0 645,648 - HGI48_RS03020 0.33 -0.9 645,674 + HGI48_RS03020 0.39 -2.3 645,674 + +2.3 645,675 - HGI48_RS03020 0.39 -0.6 645,694 - +1.4 645,729 - HGI48_RS03020 0.52 +0.4 645,915 - +1.5 645,921 + -1.5 645,921 + +0.9 645,921 + -0.5 645,922 - -1.6 645,922 - +0.7 645,922 - -2.1 645,998 + HGI48_RS03025 0.15 -1.2 645,999 - HGI48_RS03025 0.16 -1.4 645,999 - HGI48_RS03025 0.16 +1.1 645,999 - HGI48_RS03025 0.16 -2.1 645,999 - HGI48_RS03025 0.16 +0.4 646,018 + +1.7 646,030 + HGI48_RS03025 0.23 -0.9 646,030 + +1.7 646,031 - HGI48_RS03025 0.23 +0.5 646,115 + HGI48_RS03025 0.42 -1.9 646,115 + HGI48_RS03025 0.42 -1.9 646,153 + HGI48_RS03025 0.50 -1.1 646,153 + HGI48_RS03025 0.50 +0.7 646,153 + -0.3 646,153 + HGI48_RS03025 0.50 -1.9 646,153 + HGI48_RS03025 0.50 -0.2 646,153 + HGI48_RS03025 0.50 -1.4 646,154 - HGI48_RS03025 0.50 -0.1 646,154 - HGI48_RS03025 0.50 -1.4 646,154 - HGI48_RS03025 0.50 +0.3 646,154 - HGI48_RS03025 0.50 -2.0 646,154 - HGI48_RS03025 0.50 +0.4 646,167 + HGI48_RS03025 0.53 -1.3 646,293 + +2.7 646,294 - HGI48_RS03025 0.82 -1.6 646,294 - HGI48_RS03025 0.82 -0.3 646,395 + -0.1 646,395 + +1.0 646,395 + -0.6 646,395 + +0.3 646,395 + -0.1 646,396 - +4.5 646,419 + -1.6 646,435 + -1.3 646,435 + -0.4 646,435 + -2.1 646,447 + +0.2 646,476 + -1.9 646,477 - -0.9 646,611 - -0.9 646,704 + HGI48_RS03030 0.39 +0.4 646,780 - HGI48_RS03030 0.53 +0.1 646,825 + HGI48_RS03030 0.61 +0.7 646,825 + HGI48_RS03030 0.61 -0.7 646,957 - HGI48_RS03030 0.86 -1.6 646,970 + HGI48_RS03030 0.88 +1.0 646,970 + HGI48_RS03030 0.88 -1.5 646,970 + HGI48_RS03030 0.88 -1.3 646,970 + HGI48_RS03030 0.88 +1.0 646,971 - HGI48_RS03030 0.89 +1.0 646,971 - HGI48_RS03030 0.89 -1.6 646,971 - HGI48_RS03030 0.89 +0.7 646,971 - HGI48_RS03030 0.89 -0.9 646,971 - -0.3 646,971 - HGI48_RS03030 0.89 -0.4 646,971 - HGI48_RS03030 0.89 -2.3 646,974 + HGI48_RS03030 0.89 -0.3 646,974 + HGI48_RS03030 0.89 +0.4 646,974 + HGI48_RS03030 0.89 -0.6 646,975 - HGI48_RS03030 0.89 +1.2 646,975 - HGI48_RS03030 0.89 -1.9 646,994 + +0.1 646,995 - -1.7 647,011 - -1.3 647,011 - -1.3 647,011 - +0.1 647,038 - +0.4 647,038 - -1.6 647,226 + HGI48_RS03035 0.56 +0.1 647,226 + HGI48_RS03035 0.56 +0.1 647,226 + HGI48_RS03035 0.56 -1.6 647,226 + HGI48_RS03035 0.56 +0.6 647,226 + HGI48_RS03035 0.56 +1.3 647,226 + +0.1 647,226 + HGI48_RS03035 0.56 +1.0 647,227 - HGI48_RS03035 0.57 -2.2 647,227 - HGI48_RS03035 0.57 -1.1 647,227 - HGI48_RS03035 0.57 -0.3 647,227 - HGI48_RS03035 0.57 -0.1
Or see this region's nucleotide sequence