Experiment: M9+glycerol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HGI48_RS01545 and HGI48_RS01550 are separated by 4 nucleotides HGI48_RS01550 and HGI48_RS01555 are separated by 98 nucleotides HGI48_RS01555 and HGI48_RS01560 are separated by 9 nucleotides
HGI48_RS01545: HGI48_RS01545 - stringent starvation protein A, at 337,770 to 338,411
_RS01545
HGI48_RS01550: HGI48_RS01550 - ClpXP protease specificity-enhancing factor, at 338,416 to 338,913
_RS01550
HGI48_RS01555: HGI48_RS01555 - glutamate synthase small subunit, at 339,012 to 340,430
_RS01555
HGI48_RS01560: HGI48_RS01560 - glutamate synthase large subunit, at 340,440 to 344,900
_RS01560
Position (kb) 339
340
341 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 338.101 kb on - strand at 338.211 kb on + strand, within HGI48_RS01545 at 338.247 kb on + strand, within HGI48_RS01545 at 338.247 kb on + strand, within HGI48_RS01545 at 338.247 kb on + strand, within HGI48_RS01545 at 338.248 kb on - strand, within HGI48_RS01545 at 338.325 kb on + strand, within HGI48_RS01545 at 338.326 kb on - strand, within HGI48_RS01545 at 338.326 kb on - strand at 338.326 kb on - strand, within HGI48_RS01545 at 338.410 kb on - strand at 338.449 kb on + strand at 338.450 kb on - strand at 338.450 kb on - strand at 338.453 kb on + strand at 338.453 kb on + strand at 338.453 kb on + strand at 338.454 kb on - strand at 338.454 kb on - strand at 338.457 kb on - strand at 338.457 kb on - strand at 338.467 kb on + strand, within HGI48_RS01550 at 338.467 kb on + strand, within HGI48_RS01550 at 338.467 kb on + strand, within HGI48_RS01550 at 338.468 kb on - strand, within HGI48_RS01550 at 338.468 kb on - strand, within HGI48_RS01550 at 338.468 kb on - strand, within HGI48_RS01550 at 338.468 kb on - strand, within HGI48_RS01550 at 338.468 kb on - strand, within HGI48_RS01550 at 338.573 kb on + strand, within HGI48_RS01550 at 338.573 kb on + strand, within HGI48_RS01550 at 338.573 kb on + strand, within HGI48_RS01550 at 338.577 kb on + strand, within HGI48_RS01550 at 338.578 kb on - strand, within HGI48_RS01550 at 338.578 kb on - strand, within HGI48_RS01550 at 338.625 kb on - strand, within HGI48_RS01550 at 338.695 kb on + strand, within HGI48_RS01550 at 338.696 kb on - strand, within HGI48_RS01550 at 338.696 kb on - strand, within HGI48_RS01550 at 338.911 kb on + strand at 338.911 kb on + strand at 339.014 kb on - strand at 339.025 kb on + strand at 339.025 kb on + strand at 339.026 kb on - strand at 339.026 kb on - strand at 339.026 kb on - strand at 339.026 kb on - strand at 339.026 kb on - strand at 339.026 kb on - strand at 339.026 kb on - strand at 339.345 kb on + strand, within HGI48_RS01555 at 339.345 kb on + strand, within HGI48_RS01555 at 339.345 kb on + strand, within HGI48_RS01555 at 339.346 kb on - strand, within HGI48_RS01555 at 339.359 kb on + strand, within HGI48_RS01555 at 339.360 kb on - strand, within HGI48_RS01555 at 339.490 kb on - strand, within HGI48_RS01555 at 339.490 kb on - strand, within HGI48_RS01555 at 339.518 kb on + strand, within HGI48_RS01555 at 339.518 kb on + strand at 339.586 kb on + strand, within HGI48_RS01555 at 339.586 kb on + strand, within HGI48_RS01555 at 339.586 kb on + strand, within HGI48_RS01555 at 339.587 kb on - strand, within HGI48_RS01555 at 339.587 kb on - strand, within HGI48_RS01555 at 339.658 kb on + strand, within HGI48_RS01555 at 339.658 kb on + strand, within HGI48_RS01555 at 339.658 kb on + strand, within HGI48_RS01555 at 339.659 kb on - strand, within HGI48_RS01555 at 339.659 kb on - strand, within HGI48_RS01555 at 339.659 kb on - strand, within HGI48_RS01555 at 339.659 kb on - strand, within HGI48_RS01555 at 339.706 kb on + strand, within HGI48_RS01555 at 339.706 kb on + strand, within HGI48_RS01555 at 339.706 kb on + strand, within HGI48_RS01555 at 339.706 kb on + strand, within HGI48_RS01555 at 339.707 kb on - strand at 339.707 kb on - strand at 339.736 kb on + strand, within HGI48_RS01555 at 339.736 kb on + strand, within HGI48_RS01555 at 339.736 kb on + strand, within HGI48_RS01555 at 339.737 kb on - strand, within HGI48_RS01555 at 339.785 kb on + strand at 339.786 kb on - strand, within HGI48_RS01555 at 339.786 kb on - strand, within HGI48_RS01555 at 339.907 kb on + strand, within HGI48_RS01555 at 339.908 kb on - strand, within HGI48_RS01555 at 339.908 kb on - strand, within HGI48_RS01555 at 340.057 kb on + strand, within HGI48_RS01555 at 340.058 kb on - strand, within HGI48_RS01555 at 340.058 kb on - strand, within HGI48_RS01555 at 340.058 kb on - strand, within HGI48_RS01555 at 340.058 kb on - strand, within HGI48_RS01555 at 340.240 kb on + strand, within HGI48_RS01555 at 340.240 kb on + strand, within HGI48_RS01555 at 340.240 kb on + strand, within HGI48_RS01555 at 340.240 kb on + strand, within HGI48_RS01555 at 340.240 kb on + strand, within HGI48_RS01555 at 340.240 kb on + strand, within HGI48_RS01555 at 340.240 kb on + strand, within HGI48_RS01555 at 340.240 kb on + strand, within HGI48_RS01555 at 340.240 kb on + strand, within HGI48_RS01555 at 340.241 kb on - strand at 340.241 kb on - strand, within HGI48_RS01555 at 340.241 kb on - strand, within HGI48_RS01555 at 340.241 kb on - strand, within HGI48_RS01555 at 340.241 kb on - strand, within HGI48_RS01555 at 340.241 kb on - strand, within HGI48_RS01555 at 340.241 kb on - strand, within HGI48_RS01555 at 340.336 kb on + strand at 340.358 kb on + strand at 340.398 kb on + strand at 340.398 kb on + strand at 340.398 kb on + strand at 340.399 kb on - strand at 340.399 kb on - strand at 340.415 kb on - strand at 340.441 kb on + strand at 340.441 kb on + strand at 340.442 kb on - strand at 340.442 kb on - strand at 340.478 kb on + strand at 340.478 kb on + strand at 340.479 kb on - strand at 340.479 kb on - strand at 340.521 kb on + strand at 340.521 kb on + strand at 340.521 kb on + strand at 340.717 kb on + strand at 340.717 kb on + strand at 340.717 kb on + strand at 340.718 kb on - strand at 340.718 kb on - strand at 340.718 kb on - strand at 340.773 kb on - strand at 340.795 kb on + strand at 340.795 kb on + strand at 340.795 kb on + strand at 340.796 kb on - strand at 340.796 kb on - strand at 340.920 kb on - strand, within HGI48_RS01560 at 341.011 kb on + strand, within HGI48_RS01560 at 341.095 kb on + strand, within HGI48_RS01560 at 341.096 kb on - strand, within HGI48_RS01560 at 341.096 kb on - strand, within HGI48_RS01560 at 341.105 kb on + strand, within HGI48_RS01560 at 341.105 kb on + strand at 341.200 kb on + strand, within HGI48_RS01560 at 341.200 kb on + strand, within HGI48_RS01560 at 341.201 kb on - strand, within HGI48_RS01560 at 341.303 kb on - strand, within HGI48_RS01560 at 341.303 kb on - strand, within HGI48_RS01560
Per-strain Table
Position Strand Gene LocusTag Fraction M9+glycerol remove 338,101 - +1.4 338,211 + HGI48_RS01545 0.69 +0.4 338,247 + HGI48_RS01545 0.74 +0.3 338,247 + HGI48_RS01545 0.74 -0.8 338,247 + HGI48_RS01545 0.74 -1.2 338,248 - HGI48_RS01545 0.74 -2.2 338,325 + HGI48_RS01545 0.86 -2.0 338,326 - HGI48_RS01545 0.87 -2.0 338,326 - +2.0 338,326 - HGI48_RS01545 0.87 -1.8 338,410 - -1.8 338,449 + -1.3 338,450 - -2.8 338,450 - -2.2 338,453 + -0.2 338,453 + +0.2 338,453 + -2.1 338,454 - -1.8 338,454 - -1.8 338,457 - +1.4 338,457 - -1.5 338,467 + HGI48_RS01550 0.10 -1.2 338,467 + HGI48_RS01550 0.10 -1.8 338,467 + HGI48_RS01550 0.10 -2.0 338,468 - HGI48_RS01550 0.10 -0.1 338,468 - HGI48_RS01550 0.10 -1.5 338,468 - HGI48_RS01550 0.10 -1.5 338,468 - HGI48_RS01550 0.10 -2.0 338,468 - HGI48_RS01550 0.10 -0.5 338,573 + HGI48_RS01550 0.32 +0.1 338,573 + HGI48_RS01550 0.32 -1.4 338,573 + HGI48_RS01550 0.32 -0.2 338,577 + HGI48_RS01550 0.32 -0.8 338,578 - HGI48_RS01550 0.33 -2.7 338,578 - HGI48_RS01550 0.33 -2.2 338,625 - HGI48_RS01550 0.42 -2.2 338,695 + HGI48_RS01550 0.56 -0.6 338,696 - HGI48_RS01550 0.56 +0.6 338,696 - HGI48_RS01550 0.56 -0.5 338,911 + +0.1 338,911 + -1.2 339,014 - -0.8 339,025 + -1.5 339,025 + -2.4 339,026 - +0.4 339,026 - -2.0 339,026 - +0.4 339,026 - -0.1 339,026 - -2.0 339,026 - -1.5 339,026 - -1.8 339,345 + HGI48_RS01555 0.23 -0.4 339,345 + HGI48_RS01555 0.23 -2.2 339,345 + HGI48_RS01555 0.23 +2.1 339,346 - HGI48_RS01555 0.24 -2.8 339,359 + HGI48_RS01555 0.24 -0.4 339,360 - HGI48_RS01555 0.25 -2.4 339,490 - HGI48_RS01555 0.34 -2.0 339,490 - HGI48_RS01555 0.34 +0.3 339,518 + HGI48_RS01555 0.36 +1.4 339,518 + -0.2 339,586 + HGI48_RS01555 0.40 -3.4 339,586 + HGI48_RS01555 0.40 -1.0 339,586 + HGI48_RS01555 0.40 -0.3 339,587 - HGI48_RS01555 0.41 +1.2 339,587 - HGI48_RS01555 0.41 -2.6 339,658 + HGI48_RS01555 0.46 -1.8 339,658 + HGI48_RS01555 0.46 -1.8 339,658 + HGI48_RS01555 0.46 +0.4 339,659 - HGI48_RS01555 0.46 -1.8 339,659 - HGI48_RS01555 0.46 -0.8 339,659 - HGI48_RS01555 0.46 -1.2 339,659 - HGI48_RS01555 0.46 -1.2 339,706 + HGI48_RS01555 0.49 -1.2 339,706 + HGI48_RS01555 0.49 -0.7 339,706 + HGI48_RS01555 0.49 -5.0 339,706 + HGI48_RS01555 0.49 -0.2 339,707 - +2.0 339,707 - -0.8 339,736 + HGI48_RS01555 0.51 -3.2 339,736 + HGI48_RS01555 0.51 -2.0 339,736 + HGI48_RS01555 0.51 -2.2 339,737 - HGI48_RS01555 0.51 -2.4 339,785 + -0.8 339,786 - HGI48_RS01555 0.55 -4.8 339,786 - HGI48_RS01555 0.55 -0.2 339,907 + HGI48_RS01555 0.63 -1.2 339,908 - HGI48_RS01555 0.63 -2.4 339,908 - HGI48_RS01555 0.63 +1.6 340,057 + HGI48_RS01555 0.74 -2.8 340,058 - HGI48_RS01555 0.74 -2.0 340,058 - HGI48_RS01555 0.74 -3.7 340,058 - HGI48_RS01555 0.74 -3.0 340,058 - HGI48_RS01555 0.74 -1.8 340,240 + HGI48_RS01555 0.87 -2.5 340,240 + HGI48_RS01555 0.87 -1.0 340,240 + HGI48_RS01555 0.87 -1.2 340,240 + HGI48_RS01555 0.87 -2.5 340,240 + HGI48_RS01555 0.87 -2.0 340,240 + HGI48_RS01555 0.87 -2.0 340,240 + HGI48_RS01555 0.87 +0.5 340,240 + HGI48_RS01555 0.87 -0.6 340,240 + HGI48_RS01555 0.87 +0.5 340,241 - -0.2 340,241 - HGI48_RS01555 0.87 -2.4 340,241 - HGI48_RS01555 0.87 -3.5 340,241 - HGI48_RS01555 0.87 -0.9 340,241 - HGI48_RS01555 0.87 -2.0 340,241 - HGI48_RS01555 0.87 -3.5 340,241 - HGI48_RS01555 0.87 -2.2 340,336 + -0.8 340,358 + -1.2 340,398 + -2.7 340,398 + -1.5 340,398 + +0.5 340,399 - -1.8 340,399 - -2.5 340,415 - -3.5 340,441 + -1.5 340,441 + -1.2 340,442 - -0.0 340,442 - -1.2 340,478 + -2.4 340,478 + -0.8 340,479 - +1.0 340,479 - +0.5 340,521 + -0.9 340,521 + -2.7 340,521 + -2.4 340,717 + -2.0 340,717 + -1.8 340,717 + -1.2 340,718 - -1.2 340,718 - -2.4 340,718 - -1.8 340,773 - -1.8 340,795 + +0.8 340,795 + -0.6 340,795 + -2.4 340,796 - -0.8 340,796 - -1.8 340,920 - HGI48_RS01560 0.11 -1.3 341,011 + HGI48_RS01560 0.13 -1.2 341,095 + HGI48_RS01560 0.15 -1.5 341,096 - HGI48_RS01560 0.15 -2.4 341,096 - HGI48_RS01560 0.15 -2.4 341,105 + HGI48_RS01560 0.15 +1.9 341,105 + +2.6 341,200 + HGI48_RS01560 0.17 -3.0 341,200 + HGI48_RS01560 0.17 -2.5 341,201 - HGI48_RS01560 0.17 +1.2 341,303 - HGI48_RS01560 0.19 -1.3 341,303 - HGI48_RS01560 0.19 -1.8
Or see this region's nucleotide sequence