Experiment: M9+glycerol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HGI48_RS01030 and HGI48_RS01035 are separated by 683 nucleotides HGI48_RS01035 and HGI48_RS01040 are separated by 16 nucleotides HGI48_RS01040 and HGI48_RS01045 are separated by 135 nucleotides HGI48_RS01045 and HGI48_RS01050 are separated by 9 nucleotides HGI48_RS01050 and HGI48_RS01055 are separated by 106 nucleotides
HGI48_RS01030: HGI48_RS01030 - type I pantothenate kinase, at 234,201 to 235,151
_RS01030
HGI48_RS01035: HGI48_RS01035 - tRNA-Thr, at 235,835 to 235,910
_RS01035
HGI48_RS01040: HGI48_RS01040 - tRNA-Tyr, at 235,927 to 236,011
_RS01040
HGI48_RS01045: HGI48_RS01045 - tRNA-Gly, at 236,147 to 236,221
_RS01045
HGI48_RS01050: HGI48_RS01050 - tRNA-Thr, at 236,231 to 236,306
_RS01050
HGI48_RS01055: HGI48_RS01055 - elongation factor Tu, at 236,413 to 237,598
_RS01055
Position (kb) 235
236
237 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 235.152 kb on - strand at 235.158 kb on - strand at 235.158 kb on - strand at 235.158 kb on - strand at 235.328 kb on + strand at 235.350 kb on - strand at 235.390 kb on - strand at 235.417 kb on + strand at 235.417 kb on + strand at 235.417 kb on + strand at 235.417 kb on + strand at 235.420 kb on + strand at 235.421 kb on - strand at 235.421 kb on - strand at 235.452 kb on + strand at 235.452 kb on + strand at 235.452 kb on + strand at 235.452 kb on + strand at 235.453 kb on - strand at 235.453 kb on - strand at 235.453 kb on - strand at 235.461 kb on + strand at 235.462 kb on - strand at 235.462 kb on - strand at 235.462 kb on - strand at 235.504 kb on - strand at 235.525 kb on + strand at 235.526 kb on - strand at 235.546 kb on - strand at 235.618 kb on + strand at 235.619 kb on - strand at 235.668 kb on + strand at 235.668 kb on + strand at 235.668 kb on + strand at 235.685 kb on + strand at 235.686 kb on - strand at 235.705 kb on - strand at 235.705 kb on - strand at 236.035 kb on + strand at 236.035 kb on + strand at 236.035 kb on + strand at 236.047 kb on + strand at 236.224 kb on + strand at 236.225 kb on - strand at 236.225 kb on - strand at 236.238 kb on + strand at 236.239 kb on - strand at 236.239 kb on - strand, within HGI48_RS01050 at 236.239 kb on - strand, within HGI48_RS01050 at 236.239 kb on - strand, within HGI48_RS01050 at 236.313 kb on + strand at 236.314 kb on - strand at 236.340 kb on - strand at 236.355 kb on - strand at 236.355 kb on - strand at 236.377 kb on + strand at 236.461 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9+glycerol remove 235,152 - +1.0 235,158 - -0.7 235,158 - -2.2 235,158 - -1.1 235,328 + -2.2 235,350 - -0.0 235,390 - -0.5 235,417 + -2.9 235,417 + +0.6 235,417 + +0.2 235,417 + +1.8 235,420 + -0.8 235,421 - +2.0 235,421 - +0.8 235,452 + +0.4 235,452 + -0.7 235,452 + -0.8 235,452 + +1.5 235,453 - -0.0 235,453 - -1.8 235,453 - +0.4 235,461 + -1.5 235,462 - +1.3 235,462 - -1.2 235,462 - +0.5 235,504 - -1.6 235,525 + +1.1 235,526 - +0.5 235,546 - -0.8 235,618 + +0.0 235,619 - +0.8 235,668 + -0.4 235,668 + -1.4 235,668 + -1.0 235,685 + +0.9 235,686 - -0.8 235,705 - +1.4 235,705 - -0.4 236,035 + -0.2 236,035 + -1.7 236,035 + +2.4 236,047 + -1.0 236,224 + -2.0 236,225 - -2.0 236,225 - -1.2 236,238 + -1.6 236,239 - +0.2 236,239 - HGI48_RS01050 0.11 -1.2 236,239 - HGI48_RS01050 0.11 -1.8 236,239 - HGI48_RS01050 0.11 +0.9 236,313 + +1.4 236,314 - +0.1 236,340 - -1.0 236,355 - -2.1 236,355 - +0.8 236,377 + -0.5 236,461 - -1.2
Or see this region's nucleotide sequence