Experiment: M9+glycerol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HGI48_RS00620 and HGI48_RS00625 are separated by 53 nucleotides HGI48_RS00625 and HGI48_RS00630 are separated by 32 nucleotides
HGI48_RS00620: HGI48_RS00620 - isoaspartyl peptidase/L-asparaginase, at 147,358 to 148,326
_RS00620
HGI48_RS00625: HGI48_RS00625 - M55 family metallopeptidase, at 148,380 to 149,201
_RS00625
HGI48_RS00630: HGI48_RS00630 - P1 family peptidase, at 149,234 to 150,289
_RS00630
Position (kb) 148
149
150 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 147.404 kb on + strand at 147.405 kb on - strand at 147.405 kb on - strand at 147.419 kb on + strand at 147.419 kb on + strand at 147.419 kb on + strand at 147.419 kb on + strand at 147.420 kb on - strand at 147.420 kb on - strand at 147.451 kb on - strand at 147.579 kb on - strand, within HGI48_RS00620 at 147.592 kb on - strand, within HGI48_RS00620 at 147.592 kb on - strand, within HGI48_RS00620 at 147.651 kb on - strand, within HGI48_RS00620 at 148.074 kb on + strand at 148.122 kb on + strand, within HGI48_RS00620 at 148.360 kb on + strand at 148.381 kb on + strand at 148.381 kb on + strand at 148.382 kb on - strand at 148.382 kb on - strand at 148.382 kb on - strand at 148.403 kb on - strand at 148.454 kb on - strand at 148.547 kb on - strand, within HGI48_RS00625 at 148.547 kb on - strand, within HGI48_RS00625 at 148.577 kb on + strand, within HGI48_RS00625 at 148.578 kb on - strand, within HGI48_RS00625 at 148.687 kb on + strand, within HGI48_RS00625 at 148.687 kb on + strand, within HGI48_RS00625 at 148.687 kb on + strand, within HGI48_RS00625 at 148.687 kb on + strand, within HGI48_RS00625 at 148.687 kb on + strand, within HGI48_RS00625 at 148.688 kb on - strand, within HGI48_RS00625 at 148.688 kb on - strand, within HGI48_RS00625 at 148.688 kb on - strand at 148.705 kb on + strand, within HGI48_RS00625 at 148.706 kb on - strand, within HGI48_RS00625 at 148.743 kb on + strand, within HGI48_RS00625 at 148.787 kb on - strand, within HGI48_RS00625 at 148.790 kb on + strand, within HGI48_RS00625 at 148.790 kb on + strand, within HGI48_RS00625 at 148.790 kb on + strand, within HGI48_RS00625 at 148.791 kb on - strand, within HGI48_RS00625 at 148.791 kb on - strand, within HGI48_RS00625 at 149.113 kb on - strand, within HGI48_RS00625 at 149.120 kb on - strand at 149.120 kb on - strand at 149.190 kb on - strand at 149.193 kb on + strand at 149.194 kb on - strand at 149.194 kb on - strand at 149.194 kb on - strand at 149.194 kb on - strand at 149.194 kb on - strand at 149.194 kb on - strand at 149.460 kb on + strand, within HGI48_RS00630 at 149.461 kb on - strand, within HGI48_RS00630 at 149.921 kb on - strand at 149.951 kb on - strand, within HGI48_RS00630 at 150.052 kb on + strand, within HGI48_RS00630 at 150.085 kb on + strand, within HGI48_RS00630 at 150.086 kb on - strand, within HGI48_RS00630 at 150.086 kb on - strand, within HGI48_RS00630 at 150.086 kb on - strand, within HGI48_RS00630 at 150.151 kb on + strand, within HGI48_RS00630 at 150.151 kb on + strand, within HGI48_RS00630 at 150.151 kb on + strand, within HGI48_RS00630 at 150.151 kb on + strand, within HGI48_RS00630 at 150.151 kb on + strand, within HGI48_RS00630
Per-strain Table
Position Strand Gene LocusTag Fraction M9+glycerol remove 147,404 + +0.7 147,405 - +2.0 147,405 - -0.7 147,419 + -2.2 147,419 + +0.1 147,419 + -2.3 147,419 + -1.2 147,420 - -2.3 147,420 - -1.7 147,451 - -1.5 147,579 - HGI48_RS00620 0.23 +0.3 147,592 - HGI48_RS00620 0.24 +1.0 147,592 - HGI48_RS00620 0.24 -0.7 147,651 - HGI48_RS00620 0.30 -1.5 148,074 + +0.8 148,122 + HGI48_RS00620 0.79 -2.7 148,360 + -0.6 148,381 + -1.5 148,381 + +0.4 148,382 - -0.6 148,382 - +1.2 148,382 - +0.6 148,403 - +0.1 148,454 - -0.3 148,547 - HGI48_RS00625 0.20 -0.7 148,547 - HGI48_RS00625 0.20 +0.5 148,577 + HGI48_RS00625 0.24 -0.7 148,578 - HGI48_RS00625 0.24 +1.4 148,687 + HGI48_RS00625 0.37 -2.0 148,687 + HGI48_RS00625 0.37 +0.2 148,687 + HGI48_RS00625 0.37 +1.2 148,687 + HGI48_RS00625 0.37 -1.6 148,687 + HGI48_RS00625 0.37 +1.1 148,688 - HGI48_RS00625 0.37 -2.5 148,688 - HGI48_RS00625 0.37 -0.0 148,688 - +0.3 148,705 + HGI48_RS00625 0.40 -2.6 148,706 - HGI48_RS00625 0.40 -1.0 148,743 + HGI48_RS00625 0.44 -1.1 148,787 - HGI48_RS00625 0.50 -0.2 148,790 + HGI48_RS00625 0.50 -0.8 148,790 + HGI48_RS00625 0.50 -2.2 148,790 + HGI48_RS00625 0.50 -0.5 148,791 - HGI48_RS00625 0.50 +0.0 148,791 - HGI48_RS00625 0.50 +0.8 149,113 - HGI48_RS00625 0.89 +0.8 149,120 - +2.1 149,120 - +0.4 149,190 - -1.2 149,193 + -2.0 149,194 - +1.2 149,194 - +0.3 149,194 - -1.2 149,194 - -0.2 149,194 - -2.9 149,194 - +0.4 149,460 + HGI48_RS00630 0.21 -0.3 149,461 - HGI48_RS00630 0.21 +0.3 149,921 - +1.8 149,951 - HGI48_RS00630 0.68 -2.2 150,052 + HGI48_RS00630 0.77 +0.5 150,085 + HGI48_RS00630 0.81 -0.7 150,086 - HGI48_RS00630 0.81 +1.6 150,086 - HGI48_RS00630 0.81 -1.5 150,086 - HGI48_RS00630 0.81 -0.3 150,151 + HGI48_RS00630 0.87 -2.2 150,151 + HGI48_RS00630 0.87 +0.1 150,151 + HGI48_RS00630 0.87 -2.6 150,151 + HGI48_RS00630 0.87 -1.6 150,151 + HGI48_RS00630 0.87 -1.7
Or see this region's nucleotide sequence