Experiment: PDB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HGI48_RS04290 and HGI48_RS04300 are separated by 598 nucleotides HGI48_RS04300 and HGI48_RS04305 are separated by 155 nucleotides HGI48_RS04305 and HGI48_RS04310 overlap by 8 nucleotides HGI48_RS04310 and HGI48_RS04315 are separated by 58 nucleotides
HGI48_RS04290: HGI48_RS04290 - tyrosine-type recombinase/integrase, at 900,160 to 901,380
_RS04290
HGI48_RS04300: HGI48_RS04300 - SsrA-binding protein SmpB, at 901,979 to 902,461
_RS04300
HGI48_RS04305: HGI48_RS04305 - type II toxin-antitoxin system RatA family toxin, at 902,617 to 903,051
_RS04305
HGI48_RS04310: HGI48_RS04310 - RnfH family protein, at 903,044 to 903,337
_RS04310
HGI48_RS04315: HGI48_RS04315 - outer membrane protein assembly factor BamE, at 903,396 to 903,740
_RS04315
Position (kb) 901
902
903 Strain fitness (log2 ratio) -2
-1
0
1
2 at 900.988 kb on - strand, within HGI48_RS04290 at 900.993 kb on + strand at 901.010 kb on - strand, within HGI48_RS04290 at 901.148 kb on + strand, within HGI48_RS04290 at 901.148 kb on + strand, within HGI48_RS04290 at 901.148 kb on + strand, within HGI48_RS04290 at 901.149 kb on - strand, within HGI48_RS04290 at 901.149 kb on - strand, within HGI48_RS04290 at 901.187 kb on + strand, within HGI48_RS04290 at 901.188 kb on - strand, within HGI48_RS04290 at 901.193 kb on + strand at 901.194 kb on - strand, within HGI48_RS04290 at 901.194 kb on - strand, within HGI48_RS04290 at 901.194 kb on - strand, within HGI48_RS04290 at 901.240 kb on - strand, within HGI48_RS04290 at 901.241 kb on + strand, within HGI48_RS04290 at 901.242 kb on - strand, within HGI48_RS04290 at 901.242 kb on - strand, within HGI48_RS04290 at 901.369 kb on + strand at 901.369 kb on + strand at 901.369 kb on + strand at 901.370 kb on - strand at 901.370 kb on - strand at 901.419 kb on + strand at 901.422 kb on + strand at 901.422 kb on + strand at 901.422 kb on + strand at 901.422 kb on + strand at 901.423 kb on - strand at 901.423 kb on - strand at 901.423 kb on - strand at 901.449 kb on + strand at 901.462 kb on + strand at 901.602 kb on + strand at 901.602 kb on + strand at 901.602 kb on + strand at 901.602 kb on + strand at 901.603 kb on - strand at 901.749 kb on + strand at 901.806 kb on + strand at 901.945 kb on - strand at 901.947 kb on - strand at 901.966 kb on - strand at 901.966 kb on - strand at 901.971 kb on - strand at 902.197 kb on + strand at 902.271 kb on + strand, within HGI48_RS04300 at 902.271 kb on + strand, within HGI48_RS04300 at 902.298 kb on + strand, within HGI48_RS04300 at 902.298 kb on + strand, within HGI48_RS04300 at 902.298 kb on + strand, within HGI48_RS04300 at 902.299 kb on - strand at 902.311 kb on + strand, within HGI48_RS04300 at 902.461 kb on + strand at 902.462 kb on - strand at 902.462 kb on - strand at 902.473 kb on + strand at 902.474 kb on - strand at 902.474 kb on - strand at 902.474 kb on - strand at 902.474 kb on - strand at 902.474 kb on - strand at 902.474 kb on - strand at 902.474 kb on - strand at 902.474 kb on - strand at 902.580 kb on + strand at 902.598 kb on - strand at 902.616 kb on + strand at 902.668 kb on + strand, within HGI48_RS04305 at 902.815 kb on - strand at 902.835 kb on + strand at 902.916 kb on + strand, within HGI48_RS04305 at 902.916 kb on + strand, within HGI48_RS04305 at 902.916 kb on + strand, within HGI48_RS04305 at 902.917 kb on - strand at 903.040 kb on + strand at 903.041 kb on - strand at 903.041 kb on - strand at 903.041 kb on - strand at 903.140 kb on - strand, within HGI48_RS04310 at 903.295 kb on - strand at 903.397 kb on + strand at 903.397 kb on + strand at 903.398 kb on - strand at 903.398 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction PDB remove 900,988 - HGI48_RS04290 0.68 -0.3 900,993 + -0.9 901,010 - HGI48_RS04290 0.70 -0.2 901,148 + HGI48_RS04290 0.81 -0.0 901,148 + HGI48_RS04290 0.81 +0.7 901,148 + HGI48_RS04290 0.81 +1.1 901,149 - HGI48_RS04290 0.81 -0.1 901,149 - HGI48_RS04290 0.81 -1.4 901,187 + HGI48_RS04290 0.84 -1.6 901,188 - HGI48_RS04290 0.84 +0.7 901,193 + +2.0 901,194 - HGI48_RS04290 0.85 +1.8 901,194 - HGI48_RS04290 0.85 +0.2 901,194 - HGI48_RS04290 0.85 +1.3 901,240 - HGI48_RS04290 0.88 -0.1 901,241 + HGI48_RS04290 0.89 +0.5 901,242 - HGI48_RS04290 0.89 -2.7 901,242 - HGI48_RS04290 0.89 +0.1 901,369 + +0.6 901,369 + +0.9 901,369 + -1.2 901,370 - +1.6 901,370 - +1.6 901,419 + +0.8 901,422 + -1.7 901,422 + -0.0 901,422 + -0.4 901,422 + +1.3 901,423 - -1.5 901,423 - -2.1 901,423 - +0.9 901,449 + +0.3 901,462 + +1.2 901,602 + -1.7 901,602 + +1.8 901,602 + -0.7 901,602 + +1.7 901,603 - -2.0 901,749 + -0.6 901,806 + -1.4 901,945 - -0.8 901,947 - -1.7 901,966 - -0.4 901,966 - -1.5 901,971 - +1.0 902,197 + +0.0 902,271 + HGI48_RS04300 0.60 +1.1 902,271 + HGI48_RS04300 0.60 -0.4 902,298 + HGI48_RS04300 0.66 -0.1 902,298 + HGI48_RS04300 0.66 -0.8 902,298 + HGI48_RS04300 0.66 -1.4 902,299 - +0.6 902,311 + HGI48_RS04300 0.69 +1.1 902,461 + -1.4 902,462 - -0.8 902,462 - +0.5 902,473 + +1.6 902,474 - -2.4 902,474 - -0.8 902,474 - -0.0 902,474 - +0.6 902,474 - +0.6 902,474 - +0.1 902,474 - -0.8 902,474 - +0.4 902,580 + +0.9 902,598 - +0.2 902,616 + +0.2 902,668 + HGI48_RS04305 0.12 +1.0 902,815 - +2.2 902,835 + +2.1 902,916 + HGI48_RS04305 0.69 +0.2 902,916 + HGI48_RS04305 0.69 +0.4 902,916 + HGI48_RS04305 0.69 +0.7 902,917 - +2.1 903,040 + +1.2 903,041 - +0.1 903,041 - +0.9 903,041 - +1.4 903,140 - HGI48_RS04310 0.33 -0.7 903,295 - -1.0 903,397 + -0.4 903,397 + -0.2 903,398 - +0.2 903,398 - +0.9
Or see this region's nucleotide sequence