Experiment: PDB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HGI48_RS01315 and HGI48_RS01320 are separated by 11 nucleotides HGI48_RS01320 and HGI48_RS01325 are separated by 35 nucleotides HGI48_RS01325 and HGI48_RS01330 are separated by 238 nucleotides
HGI48_RS01315: HGI48_RS01315 - acetyl-CoA carboxylase biotin carboxylase subunit, at 292,447 to 293,790
_RS01315
HGI48_RS01320: HGI48_RS01320 - acetyl-CoA carboxylase biotin carboxyl carrier protein, at 293,802 to 294,263
_RS01320
HGI48_RS01325: HGI48_RS01325 - type II 3-dehydroquinate dehydratase, at 294,299 to 294,751
_RS01325
HGI48_RS01330: HGI48_RS01330 - protein-methionine-sulfoxide reductase heme-binding subunit MsrQ, at 294,990 to 295,589
_RS01330
Position (kb) 293
294
295 Strain fitness (log2 ratio) -1
0
1
2 at 294.864 kb on + strand at 294.865 kb on - strand at 294.865 kb on - strand at 294.865 kb on - strand at 294.867 kb on - strand at 294.867 kb on - strand at 294.867 kb on - strand at 294.867 kb on - strand at 294.905 kb on - strand at 295.054 kb on + strand at 295.055 kb on - strand at 295.083 kb on + strand, within HGI48_RS01330 at 295.097 kb on - strand at 295.126 kb on + strand, within HGI48_RS01330 at 295.127 kb on - strand at 295.136 kb on + strand, within HGI48_RS01330 at 295.137 kb on - strand, within HGI48_RS01330 at 295.209 kb on + strand, within HGI48_RS01330
Per-strain Table
Position Strand Gene LocusTag Fraction PDB remove 294,864 + +0.9 294,865 - +0.0 294,865 - +0.7 294,865 - +1.0 294,867 - +0.9 294,867 - +1.3 294,867 - +2.3 294,867 - +1.3 294,905 - +2.0 295,054 + +1.2 295,055 - +2.2 295,083 + HGI48_RS01330 0.15 -0.4 295,097 - +1.6 295,126 + HGI48_RS01330 0.23 -1.2 295,127 - +0.2 295,136 + HGI48_RS01330 0.24 -0.5 295,137 - HGI48_RS01330 0.24 +0.3 295,209 + HGI48_RS01330 0.36 -1.1
Or see this region's nucleotide sequence