Experiment: PDB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HGI48_RS15175 and HGI48_RS15180 are separated by 10 nucleotides HGI48_RS15180 and HGI48_RS15185 are separated by 224 nucleotides HGI48_RS15185 and HGI48_RS15190 overlap by 4 nucleotides
HGI48_RS15175: HGI48_RS15175 - peptidylprolyl isomerase B, at 3,415,979 to 3,416,473
_RS15175
HGI48_RS15180: HGI48_RS15180 - UDP-2,3-diacylglucosamine diphosphatase, at 3,416,484 to 3,417,206
_RS15180
HGI48_RS15185: HGI48_RS15185 - 5-(carboxyamino)imidazole ribonucleotide mutase, at 3,417,431 to 3,417,940
_RS15185
HGI48_RS15190: HGI48_RS15190 - 5-(carboxyamino)imidazole ribonucleotide synthase, at 3,417,937 to 3,419,007
_RS15190
Position (kb)
3417
3418 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3416.471 kb on + strand at 3417.234 kb on + strand at 3417.286 kb on + strand at 3417.287 kb on - strand at 3417.287 kb on - strand at 3417.287 kb on - strand at 3417.311 kb on - strand at 3417.337 kb on + strand at 3417.424 kb on + strand at 3417.424 kb on + strand at 3417.625 kb on + strand, within HGI48_RS15185 at 3417.625 kb on + strand, within HGI48_RS15185 at 3417.743 kb on + strand at 3417.781 kb on + strand, within HGI48_RS15185 at 3417.954 kb on - strand at 3417.978 kb on + strand at 3417.978 kb on + strand at 3417.978 kb on + strand at 3417.978 kb on + strand at 3417.979 kb on - strand at 3417.979 kb on - strand at 3417.979 kb on - strand at 3418.153 kb on - strand, within HGI48_RS15190 at 3418.177 kb on + strand, within HGI48_RS15190 at 3418.177 kb on + strand, within HGI48_RS15190 at 3418.356 kb on + strand, within HGI48_RS15190 at 3418.356 kb on + strand, within HGI48_RS15190 at 3418.357 kb on - strand, within HGI48_RS15190 at 3418.357 kb on - strand, within HGI48_RS15190 at 3418.357 kb on - strand, within HGI48_RS15190 at 3418.357 kb on - strand at 3418.465 kb on + strand, within HGI48_RS15190 at 3418.591 kb on + strand, within HGI48_RS15190 at 3418.863 kb on - strand, within HGI48_RS15190 at 3418.863 kb on - strand, within HGI48_RS15190 at 3418.889 kb on + strand, within HGI48_RS15190 at 3418.889 kb on + strand, within HGI48_RS15190 at 3418.890 kb on - strand, within HGI48_RS15190
Per-strain Table
Position Strand Gene LocusTag Fraction PDB remove 3,416,471 + +0.1 3,417,234 + +1.0 3,417,286 + -1.0 3,417,287 - -0.8 3,417,287 - -1.6 3,417,287 - -1.5 3,417,311 - +0.2 3,417,337 + +0.1 3,417,424 + +0.5 3,417,424 + +0.5 3,417,625 + HGI48_RS15185 0.38 -0.7 3,417,625 + HGI48_RS15185 0.38 -1.5 3,417,743 + -0.0 3,417,781 + HGI48_RS15185 0.69 -2.3 3,417,954 - +1.1 3,417,978 + -2.6 3,417,978 + -0.7 3,417,978 + -1.8 3,417,978 + -4.0 3,417,979 - -0.5 3,417,979 - +1.9 3,417,979 - +0.2 3,418,153 - HGI48_RS15190 0.20 -1.8 3,418,177 + HGI48_RS15190 0.22 -0.9 3,418,177 + HGI48_RS15190 0.22 -3.0 3,418,356 + HGI48_RS15190 0.39 -0.9 3,418,356 + HGI48_RS15190 0.39 +0.9 3,418,357 - HGI48_RS15190 0.39 -1.1 3,418,357 - HGI48_RS15190 0.39 -2.6 3,418,357 - HGI48_RS15190 0.39 -1.6 3,418,357 - -1.0 3,418,465 + HGI48_RS15190 0.49 -1.5 3,418,591 + HGI48_RS15190 0.61 +0.1 3,418,863 - HGI48_RS15190 0.86 +1.1 3,418,863 - HGI48_RS15190 0.86 -2.0 3,418,889 + HGI48_RS15190 0.89 -0.0 3,418,889 + HGI48_RS15190 0.89 -2.0 3,418,890 - HGI48_RS15190 0.89 -1.7
Or see this region's nucleotide sequence