Experiment: PDB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HGI48_RS07470 and HGI48_RS07475 are separated by 24 nucleotides HGI48_RS07475 and HGI48_RS07480 are separated by 32 nucleotides HGI48_RS07480 and HGI48_RS07485 overlap by 1 nucleotides HGI48_RS07485 and HGI48_RS07490 are separated by 18 nucleotides
HGI48_RS07470: HGI48_RS07470 - phosphonate C-P lyase system protein PhnK, at 1,590,506 to 1,591,309
_RS07470
HGI48_RS07475: HGI48_RS07475 - phosphonate C-P lyase system protein PhnL, at 1,591,334 to 1,592,056
_RS07475
HGI48_RS07480: HGI48_RS07480 - alpha-D-ribose 1-methylphosphonate 5-triphosphate diphosphatase, at 1,592,089 to 1,593,258
_RS07480
HGI48_RS07485: HGI48_RS07485 - ribose 1,5-bisphosphokinase, at 1,593,258 to 1,593,818
_RS07485
HGI48_RS07490: HGI48_RS07490 - phosphonate metabolism protein PhnP, at 1,593,837 to 1,594,586
_RS07490
Position (kb)
1592
1593
1594 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1591.274 kb on + strand at 1591.274 kb on + strand at 1591.275 kb on - strand at 1591.275 kb on - strand at 1591.275 kb on - strand at 1591.275 kb on - strand at 1591.308 kb on - strand at 1591.385 kb on - strand at 1591.395 kb on + strand at 1591.395 kb on + strand at 1591.395 kb on + strand at 1591.395 kb on + strand at 1591.395 kb on + strand at 1591.396 kb on - strand at 1591.402 kb on + strand at 1591.532 kb on + strand, within HGI48_RS07475 at 1592.036 kb on - strand at 1592.076 kb on + strand at 1592.076 kb on + strand at 1592.077 kb on - strand at 1592.079 kb on + strand at 1592.079 kb on + strand at 1592.080 kb on - strand at 1592.080 kb on - strand at 1592.098 kb on - strand at 1592.115 kb on + strand at 1592.116 kb on - strand at 1592.116 kb on - strand at 1592.118 kb on + strand at 1592.134 kb on - strand at 1592.134 kb on - strand at 1592.134 kb on - strand at 1592.134 kb on - strand at 1592.162 kb on + strand at 1592.162 kb on + strand at 1592.163 kb on - strand at 1592.287 kb on - strand, within HGI48_RS07480 at 1592.654 kb on - strand, within HGI48_RS07480 at 1592.662 kb on + strand, within HGI48_RS07480 at 1592.663 kb on - strand, within HGI48_RS07480 at 1592.665 kb on + strand, within HGI48_RS07480 at 1592.666 kb on - strand, within HGI48_RS07480 at 1592.666 kb on - strand, within HGI48_RS07480 at 1592.677 kb on + strand, within HGI48_RS07480 at 1592.677 kb on + strand, within HGI48_RS07480 at 1592.678 kb on - strand, within HGI48_RS07480 at 1592.678 kb on - strand, within HGI48_RS07480 at 1592.818 kb on - strand, within HGI48_RS07480 at 1592.984 kb on - strand, within HGI48_RS07480 at 1593.026 kb on - strand, within HGI48_RS07480 at 1593.029 kb on - strand at 1593.078 kb on + strand, within HGI48_RS07480 at 1593.079 kb on - strand, within HGI48_RS07480 at 1593.079 kb on - strand, within HGI48_RS07480 at 1593.087 kb on + strand, within HGI48_RS07480 at 1593.088 kb on - strand, within HGI48_RS07480 at 1593.088 kb on - strand, within HGI48_RS07480 at 1593.216 kb on - strand at 1593.363 kb on + strand, within HGI48_RS07485 at 1593.478 kb on - strand, within HGI48_RS07485 at 1593.483 kb on + strand, within HGI48_RS07485 at 1593.579 kb on + strand, within HGI48_RS07485 at 1593.593 kb on - strand, within HGI48_RS07485 at 1593.613 kb on + strand, within HGI48_RS07485 at 1593.735 kb on - strand, within HGI48_RS07485 at 1593.735 kb on - strand, within HGI48_RS07485 at 1593.822 kb on + strand at 1593.830 kb on + strand at 1593.830 kb on + strand at 1593.831 kb on - strand at 1593.831 kb on - strand at 1593.831 kb on - strand at 1593.831 kb on - strand at 1593.831 kb on - strand at 1593.837 kb on - strand at 1594.060 kb on - strand, within HGI48_RS07490 at 1594.161 kb on + strand, within HGI48_RS07490 at 1594.161 kb on + strand, within HGI48_RS07490 at 1594.162 kb on - strand, within HGI48_RS07490 at 1594.164 kb on - strand, within HGI48_RS07490 at 1594.164 kb on - strand, within HGI48_RS07490 at 1594.164 kb on - strand, within HGI48_RS07490 at 1594.164 kb on - strand, within HGI48_RS07490
Per-strain Table
Position Strand Gene LocusTag Fraction PDB remove 1,591,274 + -0.8 1,591,274 + -0.1 1,591,275 - +1.9 1,591,275 - -0.5 1,591,275 - +0.0 1,591,275 - +1.5 1,591,308 - -0.4 1,591,385 - -2.1 1,591,395 + -1.9 1,591,395 + -1.9 1,591,395 + -0.5 1,591,395 + +0.5 1,591,395 + +0.2 1,591,396 - +0.8 1,591,402 + +0.1 1,591,532 + HGI48_RS07475 0.27 +0.5 1,592,036 - -3.0 1,592,076 + -1.5 1,592,076 + -0.1 1,592,077 - +0.5 1,592,079 + +0.2 1,592,079 + -0.4 1,592,080 - -0.5 1,592,080 - +2.0 1,592,098 - +0.1 1,592,115 + +1.5 1,592,116 - -0.5 1,592,116 - -0.2 1,592,118 + +0.0 1,592,134 - +0.8 1,592,134 - -2.1 1,592,134 - -1.1 1,592,134 - -0.2 1,592,162 + -0.8 1,592,162 + +1.9 1,592,163 - -0.7 1,592,287 - HGI48_RS07480 0.17 -1.9 1,592,654 - HGI48_RS07480 0.48 +0.0 1,592,662 + HGI48_RS07480 0.49 -0.9 1,592,663 - HGI48_RS07480 0.49 -0.8 1,592,665 + HGI48_RS07480 0.49 -2.7 1,592,666 - HGI48_RS07480 0.49 -2.7 1,592,666 - HGI48_RS07480 0.49 +1.6 1,592,677 + HGI48_RS07480 0.50 -0.3 1,592,677 + HGI48_RS07480 0.50 -1.7 1,592,678 - HGI48_RS07480 0.50 +0.5 1,592,678 - HGI48_RS07480 0.50 +0.7 1,592,818 - HGI48_RS07480 0.62 -0.8 1,592,984 - HGI48_RS07480 0.76 -0.9 1,593,026 - HGI48_RS07480 0.80 -2.3 1,593,029 - -0.5 1,593,078 + HGI48_RS07480 0.85 -1.9 1,593,079 - HGI48_RS07480 0.85 -1.0 1,593,079 - HGI48_RS07480 0.85 -1.9 1,593,087 + HGI48_RS07480 0.85 +0.2 1,593,088 - HGI48_RS07480 0.85 -0.9 1,593,088 - HGI48_RS07480 0.85 -1.2 1,593,216 - +0.9 1,593,363 + HGI48_RS07485 0.19 -0.4 1,593,478 - HGI48_RS07485 0.39 +0.2 1,593,483 + HGI48_RS07485 0.40 -0.0 1,593,579 + HGI48_RS07485 0.57 -2.1 1,593,593 - HGI48_RS07485 0.60 -0.5 1,593,613 + HGI48_RS07485 0.63 -0.3 1,593,735 - HGI48_RS07485 0.85 +0.7 1,593,735 - HGI48_RS07485 0.85 -0.5 1,593,822 + +0.3 1,593,830 + -2.1 1,593,830 + -0.4 1,593,831 - -0.9 1,593,831 - +1.3 1,593,831 - +0.0 1,593,831 - +0.9 1,593,831 - +1.0 1,593,837 - +0.5 1,594,060 - HGI48_RS07490 0.30 +1.8 1,594,161 + HGI48_RS07490 0.43 -0.8 1,594,161 + HGI48_RS07490 0.43 -0.3 1,594,162 - HGI48_RS07490 0.43 -0.5 1,594,164 - HGI48_RS07490 0.44 +1.5 1,594,164 - HGI48_RS07490 0.44 -1.1 1,594,164 - HGI48_RS07490 0.44 +1.0 1,594,164 - HGI48_RS07490 0.44 -0.7
Or see this region's nucleotide sequence