Experiment: PDB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HGI48_RS05445 and HGI48_RS05450 are separated by 301 nucleotides HGI48_RS05450 and HGI48_RS05455 are separated by 36 nucleotides HGI48_RS05455 and HGI48_RS05460 are separated by 597 nucleotides
HGI48_RS05445: HGI48_RS05445 - asparaginase, at 1,141,682 to 1,142,728
_RS05445
HGI48_RS05450: HGI48_RS05450 - shikimate kinase AroL, at 1,143,030 to 1,143,551
_RS05450
HGI48_RS05455: HGI48_RS05455 - pyrimidine/purine nucleoside phosphorylase, at 1,143,588 to 1,143,872
_RS05455
HGI48_RS05460: HGI48_RS05460 - HAMP domain-containing protein, at 1,144,470 to 1,146,260
_RS05460
Position (kb)
1143
1144 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1142.712 kb on - strand at 1142.723 kb on + strand at 1142.723 kb on + strand at 1142.724 kb on - strand at 1142.797 kb on + strand at 1142.842 kb on - strand at 1142.921 kb on + strand at 1142.922 kb on - strand at 1142.931 kb on + strand at 1142.940 kb on + strand at 1142.941 kb on - strand at 1142.941 kb on - strand at 1142.957 kb on + strand at 1143.013 kb on + strand at 1143.014 kb on - strand at 1143.048 kb on - strand at 1143.112 kb on - strand, within HGI48_RS05450 at 1143.137 kb on + strand, within HGI48_RS05450 at 1143.137 kb on + strand, within HGI48_RS05450 at 1143.146 kb on + strand, within HGI48_RS05450 at 1143.146 kb on + strand, within HGI48_RS05450 at 1143.147 kb on - strand, within HGI48_RS05450 at 1143.147 kb on - strand, within HGI48_RS05450 at 1143.299 kb on + strand, within HGI48_RS05450 at 1143.300 kb on - strand at 1143.320 kb on + strand, within HGI48_RS05450 at 1143.320 kb on + strand, within HGI48_RS05450 at 1143.321 kb on - strand, within HGI48_RS05450 at 1143.324 kb on + strand, within HGI48_RS05450 at 1143.325 kb on - strand at 1143.474 kb on + strand, within HGI48_RS05450 at 1143.474 kb on + strand, within HGI48_RS05450 at 1143.474 kb on + strand, within HGI48_RS05450 at 1143.474 kb on + strand, within HGI48_RS05450 at 1143.474 kb on + strand, within HGI48_RS05450 at 1143.474 kb on + strand, within HGI48_RS05450 at 1143.474 kb on + strand, within HGI48_RS05450 at 1143.475 kb on - strand at 1143.475 kb on - strand, within HGI48_RS05450 at 1143.475 kb on - strand, within HGI48_RS05450 at 1143.475 kb on - strand, within HGI48_RS05450 at 1143.475 kb on - strand, within HGI48_RS05450 at 1143.598 kb on + strand at 1143.599 kb on - strand at 1143.622 kb on + strand, within HGI48_RS05455 at 1143.691 kb on - strand, within HGI48_RS05455 at 1143.691 kb on - strand at 1143.691 kb on - strand, within HGI48_RS05455 at 1143.692 kb on + strand, within HGI48_RS05455 at 1143.692 kb on + strand, within HGI48_RS05455 at 1143.779 kb on - strand, within HGI48_RS05455 at 1143.852 kb on + strand at 1143.852 kb on + strand at 1143.853 kb on - strand at 1143.936 kb on - strand at 1143.938 kb on + strand at 1143.938 kb on + strand at 1143.939 kb on - strand at 1143.957 kb on + strand at 1143.958 kb on - strand at 1143.959 kb on + strand at 1143.960 kb on - strand at 1143.960 kb on - strand at 1143.960 kb on - strand at 1143.960 kb on - strand at 1143.980 kb on - strand at 1144.009 kb on - strand at 1144.050 kb on + strand at 1144.050 kb on + strand at 1144.051 kb on - strand at 1144.051 kb on - strand at 1144.051 kb on - strand at 1144.076 kb on + strand at 1144.077 kb on - strand at 1144.083 kb on - strand at 1144.136 kb on + strand at 1144.137 kb on - strand at 1144.138 kb on + strand at 1144.150 kb on + strand at 1144.150 kb on + strand at 1144.151 kb on - strand at 1144.154 kb on + strand at 1144.154 kb on + strand at 1144.155 kb on - strand at 1144.217 kb on + strand at 1144.218 kb on - strand at 1144.290 kb on - strand at 1144.301 kb on + strand at 1144.301 kb on + strand at 1144.302 kb on - strand at 1144.302 kb on - strand at 1144.358 kb on + strand at 1144.358 kb on + strand at 1144.359 kb on - strand at 1144.365 kb on + strand at 1144.365 kb on + strand at 1144.365 kb on + strand at 1144.365 kb on + strand at 1144.366 kb on - strand at 1144.366 kb on - strand at 1144.366 kb on - strand at 1144.366 kb on - strand at 1144.366 kb on - strand at 1144.409 kb on - strand at 1144.467 kb on - strand at 1144.469 kb on + strand at 1144.470 kb on - strand at 1144.470 kb on - strand at 1144.475 kb on + strand at 1144.475 kb on + strand at 1144.475 kb on + strand at 1144.475 kb on + strand at 1144.476 kb on - strand at 1144.476 kb on - strand at 1144.476 kb on - strand at 1144.481 kb on + strand at 1144.494 kb on - strand at 1144.538 kb on + strand at 1144.539 kb on - strand at 1144.539 kb on - strand at 1144.542 kb on - strand at 1144.549 kb on + strand at 1144.572 kb on + strand at 1144.573 kb on - strand at 1144.573 kb on - strand at 1144.573 kb on - strand at 1144.573 kb on - strand at 1144.575 kb on - strand at 1144.575 kb on - strand at 1144.586 kb on + strand at 1144.667 kb on + strand, within HGI48_RS05460 at 1144.667 kb on + strand, within HGI48_RS05460 at 1144.668 kb on - strand, within HGI48_RS05460 at 1144.668 kb on - strand, within HGI48_RS05460 at 1144.755 kb on + strand, within HGI48_RS05460 at 1144.755 kb on + strand, within HGI48_RS05460 at 1144.755 kb on + strand, within HGI48_RS05460 at 1144.755 kb on + strand, within HGI48_RS05460 at 1144.755 kb on + strand, within HGI48_RS05460 at 1144.755 kb on + strand, within HGI48_RS05460 at 1144.756 kb on - strand, within HGI48_RS05460 at 1144.756 kb on - strand, within HGI48_RS05460 at 1144.756 kb on - strand, within HGI48_RS05460 at 1144.756 kb on - strand, within HGI48_RS05460 at 1144.757 kb on + strand, within HGI48_RS05460 at 1144.757 kb on + strand, within HGI48_RS05460 at 1144.815 kb on + strand at 1144.815 kb on + strand, within HGI48_RS05460 at 1144.815 kb on + strand, within HGI48_RS05460 at 1144.815 kb on + strand, within HGI48_RS05460 at 1144.816 kb on - strand, within HGI48_RS05460 at 1144.816 kb on - strand at 1144.816 kb on - strand, within HGI48_RS05460 at 1144.816 kb on - strand at 1144.840 kb on - strand, within HGI48_RS05460
Per-strain Table
Position Strand Gene LocusTag Fraction PDB remove 1,142,712 - +3.5 1,142,723 + -0.4 1,142,723 + -0.0 1,142,724 - -0.0 1,142,797 + +0.6 1,142,842 - +2.4 1,142,921 + -0.9 1,142,922 - -1.0 1,142,931 + +0.9 1,142,940 + +0.6 1,142,941 - +0.9 1,142,941 - -1.7 1,142,957 + +0.8 1,143,013 + +0.1 1,143,014 - +0.2 1,143,048 - -1.0 1,143,112 - HGI48_RS05450 0.16 +0.6 1,143,137 + HGI48_RS05450 0.20 -0.4 1,143,137 + HGI48_RS05450 0.20 -1.1 1,143,146 + HGI48_RS05450 0.22 -1.4 1,143,146 + HGI48_RS05450 0.22 -0.2 1,143,147 - HGI48_RS05450 0.22 +0.6 1,143,147 - HGI48_RS05450 0.22 -0.7 1,143,299 + HGI48_RS05450 0.52 -0.2 1,143,300 - -1.0 1,143,320 + HGI48_RS05450 0.56 -1.5 1,143,320 + HGI48_RS05450 0.56 +0.8 1,143,321 - HGI48_RS05450 0.56 -2.2 1,143,324 + HGI48_RS05450 0.56 -0.2 1,143,325 - -1.0 1,143,474 + HGI48_RS05450 0.85 -0.0 1,143,474 + HGI48_RS05450 0.85 -0.3 1,143,474 + HGI48_RS05450 0.85 -0.4 1,143,474 + HGI48_RS05450 0.85 -0.1 1,143,474 + HGI48_RS05450 0.85 +0.4 1,143,474 + HGI48_RS05450 0.85 -1.0 1,143,474 + HGI48_RS05450 0.85 +1.6 1,143,475 - -0.4 1,143,475 - HGI48_RS05450 0.85 -0.2 1,143,475 - HGI48_RS05450 0.85 +0.1 1,143,475 - HGI48_RS05450 0.85 +1.3 1,143,475 - HGI48_RS05450 0.85 +0.7 1,143,598 + -0.4 1,143,599 - -0.2 1,143,622 + HGI48_RS05455 0.12 +0.0 1,143,691 - HGI48_RS05455 0.36 -2.0 1,143,691 - +1.0 1,143,691 - HGI48_RS05455 0.36 -0.2 1,143,692 + HGI48_RS05455 0.36 -2.2 1,143,692 + HGI48_RS05455 0.36 +0.1 1,143,779 - HGI48_RS05455 0.67 -0.9 1,143,852 + +0.3 1,143,852 + -1.2 1,143,853 - -2.0 1,143,936 - +3.2 1,143,938 + -1.6 1,143,938 + +2.2 1,143,939 - +1.2 1,143,957 + -1.0 1,143,958 - -0.3 1,143,959 + -0.4 1,143,960 - -3.0 1,143,960 - +1.3 1,143,960 - +1.2 1,143,960 - +0.3 1,143,980 - -0.0 1,144,009 - +2.4 1,144,050 + +0.8 1,144,050 + +0.6 1,144,051 - -0.4 1,144,051 - -1.2 1,144,051 - -0.6 1,144,076 + +1.2 1,144,077 - +1.3 1,144,083 - -1.0 1,144,136 + +0.1 1,144,137 - -2.7 1,144,138 + -0.6 1,144,150 + -0.3 1,144,150 + -1.0 1,144,151 - -0.4 1,144,154 + +1.6 1,144,154 + +1.0 1,144,155 - -0.6 1,144,217 + -1.3 1,144,218 - -0.9 1,144,290 - -0.4 1,144,301 + +0.4 1,144,301 + +0.6 1,144,302 - -0.2 1,144,302 - -0.0 1,144,358 + +0.7 1,144,358 + -0.5 1,144,359 - -1.0 1,144,365 + -0.0 1,144,365 + +1.3 1,144,365 + -1.4 1,144,365 + -0.4 1,144,366 - -0.8 1,144,366 - -0.3 1,144,366 - -2.9 1,144,366 - +0.1 1,144,366 - -1.4 1,144,409 - -2.4 1,144,467 - +0.7 1,144,469 + -0.6 1,144,470 - +0.1 1,144,470 - -2.8 1,144,475 + -0.2 1,144,475 + +0.1 1,144,475 + +0.4 1,144,475 + -0.2 1,144,476 - +1.1 1,144,476 - -0.4 1,144,476 - -1.4 1,144,481 + -0.4 1,144,494 - -0.6 1,144,538 + +1.0 1,144,539 - +0.2 1,144,539 - -1.7 1,144,542 - -2.0 1,144,549 + +0.4 1,144,572 + -0.6 1,144,573 - -0.4 1,144,573 - +0.3 1,144,573 - -1.0 1,144,573 - -0.4 1,144,575 - +1.1 1,144,575 - +1.4 1,144,586 + -1.3 1,144,667 + HGI48_RS05460 0.11 -0.7 1,144,667 + HGI48_RS05460 0.11 +0.2 1,144,668 - HGI48_RS05460 0.11 +1.3 1,144,668 - HGI48_RS05460 0.11 +0.6 1,144,755 + HGI48_RS05460 0.16 +0.9 1,144,755 + HGI48_RS05460 0.16 -0.2 1,144,755 + HGI48_RS05460 0.16 -3.5 1,144,755 + HGI48_RS05460 0.16 -0.3 1,144,755 + HGI48_RS05460 0.16 -2.0 1,144,755 + HGI48_RS05460 0.16 -1.7 1,144,756 - HGI48_RS05460 0.16 +1.6 1,144,756 - HGI48_RS05460 0.16 +0.1 1,144,756 - HGI48_RS05460 0.16 -1.2 1,144,756 - HGI48_RS05460 0.16 -1.7 1,144,757 + HGI48_RS05460 0.16 -0.6 1,144,757 + HGI48_RS05460 0.16 +0.9 1,144,815 + +0.6 1,144,815 + HGI48_RS05460 0.19 -1.4 1,144,815 + HGI48_RS05460 0.19 -0.4 1,144,815 + HGI48_RS05460 0.19 -0.2 1,144,816 - HGI48_RS05460 0.19 +0.9 1,144,816 - +1.3 1,144,816 - HGI48_RS05460 0.19 -0.6 1,144,816 - -1.0 1,144,840 - HGI48_RS05460 0.21 -0.7
Or see this region's nucleotide sequence