Experiment: PDB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HGI48_RS05250 and HGI48_RS05255 are separated by 30 nucleotides HGI48_RS05255 and HGI48_RS05260 are separated by 66 nucleotides HGI48_RS05260 and HGI48_RS05265 are separated by 104 nucleotides
HGI48_RS05250: HGI48_RS05250 - IS3 family transposase, at 1,102,662 to 1,103,812
_RS05250
HGI48_RS05255: HGI48_RS05255 - IS3 family transposase, at 1,103,843 to 1,103,974
_RS05255
HGI48_RS05260: HGI48_RS05260 - transposase, at 1,104,041 to 1,104,271
_RS05260
HGI48_RS05265: HGI48_RS05265 - ATP-binding protein, at 1,104,376 to 1,106,091
_RS05265
Position (kb)
1104
1105 Strain fitness (log2 ratio)
-1
0
1
2 at 1103.719 kb on + strand at 1103.827 kb on + strand at 1103.827 kb on + strand at 1103.939 kb on - strand at 1103.999 kb on + strand at 1104.059 kb on + strand at 1104.059 kb on + strand at 1104.060 kb on - strand at 1104.060 kb on - strand at 1104.060 kb on - strand at 1104.149 kb on - strand, within HGI48_RS05260 at 1104.149 kb on - strand at 1104.294 kb on + strand at 1104.491 kb on - strand at 1104.501 kb on + strand at 1104.772 kb on - strand, within HGI48_RS05265 at 1104.819 kb on - strand, within HGI48_RS05265 at 1105.032 kb on + strand, within HGI48_RS05265 at 1105.148 kb on - strand at 1105.150 kb on - strand, within HGI48_RS05265 at 1105.158 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction PDB remove 1,103,719 + +0.6 1,103,827 + -0.0 1,103,827 + -0.6 1,103,939 - +2.6 1,103,999 + +0.6 1,104,059 + -0.4 1,104,059 + -0.4 1,104,060 - +0.8 1,104,060 - -0.4 1,104,060 - -1.2 1,104,149 - HGI48_RS05260 0.47 -0.6 1,104,149 - +1.6 1,104,294 + -1.7 1,104,491 - -0.6 1,104,501 + +0.6 1,104,772 - HGI48_RS05265 0.23 -1.7 1,104,819 - HGI48_RS05265 0.26 +1.6 1,105,032 + HGI48_RS05265 0.38 +0.2 1,105,148 - -0.4 1,105,150 - HGI48_RS05265 0.45 -0.6 1,105,158 + -0.0
Or see this region's nucleotide sequence