Experiment: PDB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HGI48_RS01425 and HGI48_RS01430 overlap by 4 nucleotides HGI48_RS01430 and HGI48_RS01435 are separated by 145 nucleotides HGI48_RS01435 and HGI48_RS01440 are separated by 92 nucleotides HGI48_RS01440 and HGI48_RS01445 are separated by 24 nucleotides
HGI48_RS01425: HGI48_RS01425 - PTS phosphocarrier protein NPr, at 318,513 to 318,785
_RS01425
HGI48_RS01430: HGI48_RS01430 - RNase adapter RapZ, at 318,782 to 319,636
_RS01430
HGI48_RS01435: HGI48_RS01435 - PTS IIA-like nitrogen regulatory protein PtsN, at 319,782 to 320,270
_RS01435
HGI48_RS01440: HGI48_RS01440 - ribosome hibernation promoting factor, at 320,363 to 320,650
_RS01440
HGI48_RS01445: HGI48_RS01445 - RNA polymerase factor sigma-54, at 320,675 to 322,108
_RS01445
Position (kb) 319
320
321 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 318.870 kb on + strand at 318.871 kb on - strand, within HGI48_RS01430 at 318.914 kb on - strand, within HGI48_RS01430 at 318.914 kb on - strand, within HGI48_RS01430 at 318.918 kb on + strand, within HGI48_RS01430 at 318.918 kb on + strand, within HGI48_RS01430 at 318.918 kb on + strand, within HGI48_RS01430 at 318.919 kb on - strand, within HGI48_RS01430 at 318.919 kb on - strand, within HGI48_RS01430 at 318.919 kb on - strand, within HGI48_RS01430 at 318.978 kb on + strand, within HGI48_RS01430 at 318.978 kb on + strand at 318.979 kb on - strand, within HGI48_RS01430 at 318.979 kb on - strand, within HGI48_RS01430 at 318.979 kb on - strand, within HGI48_RS01430 at 319.101 kb on + strand at 319.101 kb on + strand, within HGI48_RS01430 at 319.101 kb on + strand, within HGI48_RS01430 at 319.153 kb on + strand, within HGI48_RS01430 at 319.557 kb on + strand at 319.558 kb on - strand at 319.558 kb on - strand at 319.558 kb on - strand at 319.558 kb on - strand at 319.590 kb on - strand at 319.684 kb on + strand at 319.685 kb on - strand at 319.685 kb on - strand at 319.741 kb on + strand at 319.742 kb on - strand at 319.742 kb on - strand at 319.904 kb on + strand at 319.904 kb on + strand, within HGI48_RS01435 at 319.904 kb on + strand, within HGI48_RS01435 at 319.905 kb on - strand, within HGI48_RS01435 at 319.905 kb on - strand, within HGI48_RS01435 at 319.905 kb on - strand, within HGI48_RS01435 at 319.905 kb on - strand, within HGI48_RS01435 at 319.905 kb on - strand, within HGI48_RS01435 at 319.905 kb on - strand, within HGI48_RS01435 at 319.909 kb on - strand, within HGI48_RS01435 at 319.909 kb on - strand, within HGI48_RS01435 at 319.909 kb on - strand, within HGI48_RS01435 at 319.909 kb on - strand, within HGI48_RS01435 at 319.909 kb on - strand, within HGI48_RS01435 at 319.919 kb on + strand, within HGI48_RS01435 at 319.920 kb on - strand at 319.953 kb on + strand, within HGI48_RS01435 at 320.034 kb on - strand, within HGI48_RS01435 at 320.220 kb on - strand, within HGI48_RS01435 at 320.244 kb on - strand at 320.273 kb on + strand at 320.274 kb on - strand at 320.274 kb on - strand at 320.274 kb on - strand at 320.274 kb on - strand at 320.321 kb on + strand at 320.322 kb on - strand at 320.322 kb on - strand at 320.386 kb on + strand at 320.387 kb on - strand at 320.439 kb on + strand, within HGI48_RS01440 at 320.439 kb on + strand, within HGI48_RS01440 at 320.439 kb on + strand at 320.440 kb on - strand, within HGI48_RS01440 at 320.440 kb on - strand, within HGI48_RS01440 at 320.443 kb on + strand, within HGI48_RS01440 at 320.539 kb on - strand, within HGI48_RS01440 at 320.551 kb on + strand, within HGI48_RS01440 at 320.689 kb on + strand at 320.711 kb on - strand at 320.907 kb on + strand, within HGI48_RS01445 at 321.017 kb on - strand at 321.202 kb on - strand, within HGI48_RS01445
Per-strain Table
Position Strand Gene LocusTag Fraction PDB remove 318,870 + +2.1 318,871 - HGI48_RS01430 0.10 -1.9 318,914 - HGI48_RS01430 0.15 -2.6 318,914 - HGI48_RS01430 0.15 +1.5 318,918 + HGI48_RS01430 0.16 +0.7 318,918 + HGI48_RS01430 0.16 -0.3 318,918 + HGI48_RS01430 0.16 -2.5 318,919 - HGI48_RS01430 0.16 -0.7 318,919 - HGI48_RS01430 0.16 -1.3 318,919 - HGI48_RS01430 0.16 -0.9 318,978 + HGI48_RS01430 0.23 -1.6 318,978 + -0.9 318,979 - HGI48_RS01430 0.23 +1.1 318,979 - HGI48_RS01430 0.23 -0.1 318,979 - HGI48_RS01430 0.23 -0.9 319,101 + +2.3 319,101 + HGI48_RS01430 0.37 -1.9 319,101 + HGI48_RS01430 0.37 +0.5 319,153 + HGI48_RS01430 0.43 -0.9 319,557 + -0.4 319,558 - -1.3 319,558 - -0.1 319,558 - -2.5 319,558 - +0.3 319,590 - -0.9 319,684 + -1.3 319,685 - -0.9 319,685 - +1.4 319,741 + +0.5 319,742 - -0.9 319,742 - +1.0 319,904 + -0.3 319,904 + HGI48_RS01435 0.25 -1.6 319,904 + HGI48_RS01435 0.25 -1.1 319,905 - HGI48_RS01435 0.25 +1.0 319,905 - HGI48_RS01435 0.25 -0.5 319,905 - HGI48_RS01435 0.25 -0.3 319,905 - HGI48_RS01435 0.25 +1.9 319,905 - HGI48_RS01435 0.25 -0.7 319,905 - HGI48_RS01435 0.25 -0.1 319,909 - HGI48_RS01435 0.26 -1.1 319,909 - HGI48_RS01435 0.26 -1.6 319,909 - HGI48_RS01435 0.26 -0.6 319,909 - HGI48_RS01435 0.26 -2.7 319,909 - HGI48_RS01435 0.26 -1.2 319,919 + HGI48_RS01435 0.28 -2.1 319,920 - +0.7 319,953 + HGI48_RS01435 0.35 -1.3 320,034 - HGI48_RS01435 0.52 +0.3 320,220 - HGI48_RS01435 0.90 +0.5 320,244 - -0.0 320,273 + +0.3 320,274 - +1.3 320,274 - -1.4 320,274 - +0.2 320,274 - +0.4 320,321 + +0.3 320,322 - -0.9 320,322 - +0.3 320,386 + -3.2 320,387 - +0.6 320,439 + HGI48_RS01440 0.26 +0.4 320,439 + HGI48_RS01440 0.26 -0.3 320,439 + -0.3 320,440 - HGI48_RS01440 0.27 +0.4 320,440 - HGI48_RS01440 0.27 -2.1 320,443 + HGI48_RS01440 0.28 -1.9 320,539 - HGI48_RS01440 0.61 +0.5 320,551 + HGI48_RS01440 0.65 +0.4 320,689 + -0.3 320,711 - -0.9 320,907 + HGI48_RS01445 0.16 +0.7 321,017 - -0.3 321,202 - HGI48_RS01445 0.37 -1.3
Or see this region's nucleotide sequence