Experiment: PDB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HGI48_RS18485 and HGI48_RS18490 are separated by 127 nucleotides HGI48_RS18490 and HGI48_RS18495 are separated by 11 nucleotides HGI48_RS18495 and HGI48_RS18500 are separated by 104 nucleotides HGI48_RS18500 and HGI48_RS18505 overlap by 1 nucleotides
HGI48_RS18485: HGI48_RS18485 - deoxyribonuclease I, at 4,150,512 to 4,151,210
_RS18485
HGI48_RS18490: HGI48_RS18490 - 16S rRNA (uracil(1498)-N(3))-methyltransferase, at 4,151,338 to 4,152,072
_RS18490
HGI48_RS18495: HGI48_RS18495 - glutathione synthase, at 4,152,084 to 4,153,037
_RS18495
HGI48_RS18500: HGI48_RS18500 - YqgE/AlgH family protein, at 4,153,142 to 4,153,705
_RS18500
HGI48_RS18505: HGI48_RS18505 - Holliday junction resolvase RuvX, at 4,153,705 to 4,154,136
_RS18505
Position (kb)
4152
4153
4154 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4151.138 kb on + strand at 4151.184 kb on + strand at 4151.184 kb on + strand at 4151.184 kb on + strand at 4151.185 kb on - strand at 4151.185 kb on - strand at 4151.185 kb on - strand at 4151.221 kb on + strand at 4151.222 kb on - strand at 4151.224 kb on + strand at 4151.224 kb on + strand at 4151.224 kb on + strand at 4151.224 kb on + strand at 4151.224 kb on + strand at 4151.225 kb on - strand at 4151.225 kb on - strand at 4151.225 kb on - strand at 4151.225 kb on - strand at 4151.225 kb on - strand at 4151.225 kb on - strand at 4151.241 kb on + strand at 4151.241 kb on + strand at 4151.241 kb on + strand at 4151.241 kb on + strand at 4151.242 kb on - strand at 4151.242 kb on - strand at 4151.242 kb on - strand at 4151.243 kb on + strand at 4151.244 kb on - strand at 4151.244 kb on - strand at 4151.244 kb on - strand at 4151.244 kb on - strand at 4151.244 kb on - strand at 4151.244 kb on - strand at 4151.322 kb on + strand at 4151.322 kb on + strand at 4151.322 kb on + strand at 4151.322 kb on + strand at 4151.323 kb on - strand at 4151.333 kb on + strand at 4151.334 kb on - strand at 4151.334 kb on - strand at 4151.345 kb on + strand at 4151.346 kb on - strand at 4151.486 kb on + strand, within HGI48_RS18490 at 4151.486 kb on + strand, within HGI48_RS18490 at 4151.486 kb on + strand, within HGI48_RS18490 at 4151.486 kb on + strand, within HGI48_RS18490 at 4151.487 kb on - strand, within HGI48_RS18490 at 4151.487 kb on - strand, within HGI48_RS18490 at 4151.537 kb on - strand, within HGI48_RS18490 at 4151.840 kb on + strand, within HGI48_RS18490 at 4151.840 kb on + strand, within HGI48_RS18490 at 4151.841 kb on - strand, within HGI48_RS18490 at 4151.977 kb on - strand, within HGI48_RS18490 at 4151.977 kb on - strand at 4151.977 kb on - strand, within HGI48_RS18490 at 4151.977 kb on - strand, within HGI48_RS18490 at 4151.982 kb on + strand, within HGI48_RS18490 at 4151.982 kb on + strand, within HGI48_RS18490 at 4151.982 kb on + strand, within HGI48_RS18490 at 4151.983 kb on - strand, within HGI48_RS18490 at 4152.050 kb on + strand at 4152.051 kb on - strand at 4152.051 kb on - strand at 4152.070 kb on + strand at 4152.079 kb on + strand at 4152.079 kb on + strand at 4152.080 kb on - strand at 4152.080 kb on - strand at 4152.090 kb on - strand at 4152.192 kb on + strand, within HGI48_RS18495 at 4152.192 kb on + strand at 4152.192 kb on + strand, within HGI48_RS18495 at 4152.192 kb on + strand, within HGI48_RS18495 at 4152.192 kb on + strand, within HGI48_RS18495 at 4152.192 kb on + strand at 4152.192 kb on + strand at 4152.193 kb on - strand, within HGI48_RS18495 at 4152.193 kb on - strand, within HGI48_RS18495 at 4152.193 kb on - strand, within HGI48_RS18495 at 4152.193 kb on - strand at 4152.193 kb on - strand, within HGI48_RS18495 at 4152.193 kb on - strand, within HGI48_RS18495 at 4152.194 kb on + strand, within HGI48_RS18495 at 4152.213 kb on + strand, within HGI48_RS18495 at 4152.213 kb on + strand, within HGI48_RS18495 at 4152.214 kb on - strand, within HGI48_RS18495 at 4152.249 kb on - strand, within HGI48_RS18495 at 4152.265 kb on - strand, within HGI48_RS18495 at 4152.265 kb on - strand, within HGI48_RS18495 at 4152.271 kb on - strand, within HGI48_RS18495 at 4152.271 kb on - strand at 4152.283 kb on - strand, within HGI48_RS18495 at 4152.372 kb on + strand, within HGI48_RS18495 at 4152.372 kb on + strand, within HGI48_RS18495 at 4152.373 kb on - strand, within HGI48_RS18495 at 4152.381 kb on + strand, within HGI48_RS18495 at 4152.381 kb on + strand, within HGI48_RS18495 at 4152.381 kb on + strand, within HGI48_RS18495 at 4152.382 kb on - strand at 4152.382 kb on - strand, within HGI48_RS18495 at 4152.382 kb on - strand, within HGI48_RS18495 at 4152.382 kb on - strand, within HGI48_RS18495 at 4152.382 kb on - strand, within HGI48_RS18495 at 4152.383 kb on + strand, within HGI48_RS18495 at 4152.383 kb on + strand, within HGI48_RS18495 at 4152.744 kb on + strand, within HGI48_RS18495 at 4152.851 kb on + strand, within HGI48_RS18495 at 4153.069 kb on + strand at 4153.069 kb on + strand at 4153.069 kb on + strand at 4153.069 kb on + strand at 4153.069 kb on + strand at 4153.069 kb on + strand at 4153.070 kb on - strand at 4153.149 kb on + strand at 4153.149 kb on + strand at 4153.214 kb on + strand, within HGI48_RS18500 at 4153.252 kb on + strand, within HGI48_RS18500 at 4153.253 kb on - strand, within HGI48_RS18500 at 4153.402 kb on + strand, within HGI48_RS18500 at 4153.402 kb on + strand, within HGI48_RS18500 at 4153.402 kb on + strand at 4153.402 kb on + strand, within HGI48_RS18500 at 4153.510 kb on + strand, within HGI48_RS18500 at 4153.510 kb on + strand, within HGI48_RS18500
Per-strain Table
Position Strand Gene LocusTag Fraction PDB remove 4,151,138 + +1.6 4,151,184 + -0.2 4,151,184 + -0.5 4,151,184 + -0.6 4,151,185 - -0.2 4,151,185 - -1.2 4,151,185 - +1.8 4,151,221 + +1.8 4,151,222 - -0.3 4,151,224 + +0.3 4,151,224 + +0.8 4,151,224 + -0.4 4,151,224 + -1.0 4,151,224 + +0.1 4,151,225 - -0.4 4,151,225 - -0.2 4,151,225 - -1.0 4,151,225 - +0.6 4,151,225 - +2.5 4,151,225 - -0.5 4,151,241 + -1.6 4,151,241 + +0.8 4,151,241 + +0.7 4,151,241 + -0.0 4,151,242 - -0.2 4,151,242 - +0.6 4,151,242 - -1.0 4,151,243 + +0.4 4,151,244 - +0.8 4,151,244 - +0.1 4,151,244 - +0.0 4,151,244 - +1.0 4,151,244 - -0.5 4,151,244 - +0.1 4,151,322 + -0.4 4,151,322 + +0.5 4,151,322 + +1.5 4,151,322 + -0.1 4,151,323 - +1.4 4,151,333 + -0.7 4,151,334 - -1.0 4,151,334 - +0.7 4,151,345 + -1.2 4,151,346 - +1.7 4,151,486 + HGI48_RS18490 0.20 -0.2 4,151,486 + HGI48_RS18490 0.20 -2.2 4,151,486 + HGI48_RS18490 0.20 -0.2 4,151,486 + HGI48_RS18490 0.20 -0.6 4,151,487 - HGI48_RS18490 0.20 -0.5 4,151,487 - HGI48_RS18490 0.20 +1.2 4,151,537 - HGI48_RS18490 0.27 +0.1 4,151,840 + HGI48_RS18490 0.68 -0.3 4,151,840 + HGI48_RS18490 0.68 +2.0 4,151,841 - HGI48_RS18490 0.68 -2.0 4,151,977 - HGI48_RS18490 0.87 +0.8 4,151,977 - +1.6 4,151,977 - HGI48_RS18490 0.87 -0.7 4,151,977 - HGI48_RS18490 0.87 -1.5 4,151,982 + HGI48_RS18490 0.88 +1.6 4,151,982 + HGI48_RS18490 0.88 -0.7 4,151,982 + HGI48_RS18490 0.88 +0.8 4,151,983 - HGI48_RS18490 0.88 +0.3 4,152,050 + -0.4 4,152,051 - -0.7 4,152,051 - -0.5 4,152,070 + -0.7 4,152,079 + +0.4 4,152,079 + -0.9 4,152,080 - -1.2 4,152,080 - -0.3 4,152,090 - +1.2 4,152,192 + HGI48_RS18495 0.11 +0.5 4,152,192 + -0.7 4,152,192 + HGI48_RS18495 0.11 +1.1 4,152,192 + HGI48_RS18495 0.11 +0.0 4,152,192 + HGI48_RS18495 0.11 -1.0 4,152,192 + -0.2 4,152,192 + -0.7 4,152,193 - HGI48_RS18495 0.11 -1.5 4,152,193 - HGI48_RS18495 0.11 +0.1 4,152,193 - HGI48_RS18495 0.11 -2.3 4,152,193 - +0.8 4,152,193 - HGI48_RS18495 0.11 +0.4 4,152,193 - HGI48_RS18495 0.11 +0.3 4,152,194 + HGI48_RS18495 0.12 +0.4 4,152,213 + HGI48_RS18495 0.14 -1.7 4,152,213 + HGI48_RS18495 0.14 -0.5 4,152,214 - HGI48_RS18495 0.14 -0.0 4,152,249 - HGI48_RS18495 0.17 -0.2 4,152,265 - HGI48_RS18495 0.19 -2.3 4,152,265 - HGI48_RS18495 0.19 -2.6 4,152,271 - HGI48_RS18495 0.20 +0.4 4,152,271 - -0.2 4,152,283 - HGI48_RS18495 0.21 +1.1 4,152,372 + HGI48_RS18495 0.30 -0.7 4,152,372 + HGI48_RS18495 0.30 -1.7 4,152,373 - HGI48_RS18495 0.30 +1.2 4,152,381 + HGI48_RS18495 0.31 -1.3 4,152,381 + HGI48_RS18495 0.31 -1.7 4,152,381 + HGI48_RS18495 0.31 -1.5 4,152,382 - -0.7 4,152,382 - HGI48_RS18495 0.31 -1.2 4,152,382 - HGI48_RS18495 0.31 +1.8 4,152,382 - HGI48_RS18495 0.31 +0.5 4,152,382 - HGI48_RS18495 0.31 +0.5 4,152,383 + HGI48_RS18495 0.31 +0.4 4,152,383 + HGI48_RS18495 0.31 -1.2 4,152,744 + HGI48_RS18495 0.69 -0.2 4,152,851 + HGI48_RS18495 0.80 -2.8 4,153,069 + +0.8 4,153,069 + +0.4 4,153,069 + -1.5 4,153,069 + -1.5 4,153,069 + +1.1 4,153,069 + -1.2 4,153,070 - +0.6 4,153,149 + -0.5 4,153,149 + -1.7 4,153,214 + HGI48_RS18500 0.13 -2.0 4,153,252 + HGI48_RS18500 0.20 -1.0 4,153,253 - HGI48_RS18500 0.20 +0.6 4,153,402 + HGI48_RS18500 0.46 +0.4 4,153,402 + HGI48_RS18500 0.46 -0.3 4,153,402 + +1.6 4,153,402 + HGI48_RS18500 0.46 -1.2 4,153,510 + HGI48_RS18500 0.65 +1.7 4,153,510 + HGI48_RS18500 0.65 +1.1
Or see this region's nucleotide sequence