Experiment: PDB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HGI48_RS10280 and HGI48_RS10285 are separated by 65 nucleotides HGI48_RS10285 and HGI48_RS10290 are separated by 45 nucleotides HGI48_RS10290 and HGI48_RS10295 are separated by 60 nucleotides
HGI48_RS10280: HGI48_RS10280 - peptide-methionine (R)-S-oxide reductase MsrB, at 2,219,090 to 2,219,506
_RS10280
HGI48_RS10285: HGI48_RS10285 - DUF1315 family protein, at 2,219,572 to 2,219,847
_RS10285
HGI48_RS10290: HGI48_RS10290 - bifunctional nicotinamidase/pyrazinamidase, at 2,219,893 to 2,220,534
_RS10290
HGI48_RS10295: HGI48_RS10295 - asparaginase, at 2,220,595 to 2,221,614
_RS10295
Position (kb)
2219
2220
2221 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2218.908 kb on - strand at 2218.916 kb on + strand at 2218.916 kb on + strand at 2218.917 kb on - strand at 2218.941 kb on + strand at 2218.941 kb on + strand at 2218.941 kb on + strand at 2218.942 kb on - strand at 2218.947 kb on + strand at 2218.995 kb on - strand at 2219.000 kb on + strand at 2219.001 kb on - strand at 2219.044 kb on - strand at 2219.116 kb on + strand at 2219.116 kb on + strand at 2219.117 kb on - strand at 2219.117 kb on - strand at 2219.131 kb on - strand at 2219.131 kb on - strand at 2219.181 kb on - strand at 2219.201 kb on - strand, within HGI48_RS10280 at 2219.267 kb on + strand, within HGI48_RS10280 at 2219.267 kb on + strand at 2219.267 kb on + strand, within HGI48_RS10280 at 2219.268 kb on - strand, within HGI48_RS10280 at 2219.268 kb on - strand, within HGI48_RS10280 at 2219.350 kb on + strand, within HGI48_RS10280 at 2219.351 kb on - strand, within HGI48_RS10280 at 2219.351 kb on - strand, within HGI48_RS10280 at 2219.351 kb on - strand, within HGI48_RS10280 at 2219.522 kb on - strand at 2219.522 kb on - strand at 2219.588 kb on + strand at 2219.589 kb on - strand at 2219.591 kb on + strand at 2219.598 kb on + strand at 2219.706 kb on + strand, within HGI48_RS10285 at 2219.706 kb on + strand, within HGI48_RS10285 at 2219.707 kb on - strand, within HGI48_RS10285 at 2219.707 kb on - strand, within HGI48_RS10285 at 2219.728 kb on - strand, within HGI48_RS10285 at 2219.817 kb on + strand, within HGI48_RS10285 at 2219.817 kb on + strand, within HGI48_RS10285 at 2219.817 kb on + strand, within HGI48_RS10285 at 2219.817 kb on + strand, within HGI48_RS10285 at 2219.818 kb on - strand, within HGI48_RS10285 at 2219.820 kb on + strand at 2219.820 kb on + strand at 2219.820 kb on + strand at 2219.821 kb on - strand at 2219.821 kb on - strand at 2219.821 kb on - strand at 2219.821 kb on - strand at 2219.845 kb on + strand at 2219.887 kb on + strand at 2219.888 kb on - strand at 2219.894 kb on + strand at 2219.895 kb on - strand at 2219.913 kb on + strand at 2219.913 kb on + strand at 2220.019 kb on + strand, within HGI48_RS10290 at 2220.019 kb on + strand, within HGI48_RS10290 at 2220.019 kb on + strand, within HGI48_RS10290 at 2220.020 kb on - strand, within HGI48_RS10290 at 2220.060 kb on + strand, within HGI48_RS10290 at 2220.062 kb on + strand, within HGI48_RS10290 at 2220.062 kb on + strand, within HGI48_RS10290 at 2220.063 kb on - strand, within HGI48_RS10290 at 2220.063 kb on - strand, within HGI48_RS10290 at 2220.134 kb on + strand, within HGI48_RS10290 at 2220.134 kb on + strand, within HGI48_RS10290 at 2220.135 kb on - strand, within HGI48_RS10290 at 2220.135 kb on - strand, within HGI48_RS10290 at 2220.150 kb on + strand, within HGI48_RS10290 at 2220.180 kb on - strand, within HGI48_RS10290 at 2220.223 kb on + strand, within HGI48_RS10290 at 2220.275 kb on - strand, within HGI48_RS10290 at 2220.517 kb on + strand at 2220.535 kb on - strand at 2220.548 kb on + strand at 2220.548 kb on + strand at 2220.548 kb on + strand at 2220.548 kb on + strand at 2220.548 kb on + strand at 2220.549 kb on - strand at 2220.549 kb on - strand at 2220.549 kb on - strand at 2220.560 kb on + strand at 2220.596 kb on + strand at 2220.596 kb on + strand at 2220.597 kb on - strand at 2220.679 kb on + strand at 2220.679 kb on + strand at 2220.680 kb on - strand at 2220.683 kb on + strand at 2220.683 kb on + strand at 2220.684 kb on - strand at 2220.717 kb on + strand, within HGI48_RS10295 at 2220.717 kb on + strand, within HGI48_RS10295 at 2220.718 kb on - strand, within HGI48_RS10295 at 2220.811 kb on - strand, within HGI48_RS10295 at 2220.815 kb on - strand, within HGI48_RS10295 at 2220.890 kb on + strand, within HGI48_RS10295 at 2220.890 kb on + strand, within HGI48_RS10295 at 2220.973 kb on + strand at 2220.974 kb on - strand, within HGI48_RS10295 at 2221.090 kb on - strand, within HGI48_RS10295 at 2221.205 kb on + strand, within HGI48_RS10295 at 2221.205 kb on + strand, within HGI48_RS10295 at 2221.206 kb on - strand, within HGI48_RS10295 at 2221.370 kb on + strand at 2221.370 kb on + strand, within HGI48_RS10295 at 2221.380 kb on - strand, within HGI48_RS10295 at 2221.450 kb on + strand at 2221.451 kb on - strand, within HGI48_RS10295 at 2221.451 kb on - strand, within HGI48_RS10295 at 2221.454 kb on + strand, within HGI48_RS10295 at 2221.454 kb on + strand, within HGI48_RS10295 at 2221.454 kb on + strand at 2221.455 kb on - strand, within HGI48_RS10295 at 2221.455 kb on - strand, within HGI48_RS10295 at 2221.455 kb on - strand, within HGI48_RS10295 at 2221.455 kb on - strand, within HGI48_RS10295 at 2221.455 kb on - strand at 2221.455 kb on - strand, within HGI48_RS10295
Per-strain Table
Position Strand Gene LocusTag Fraction PDB remove 2,218,908 - -0.3 2,218,916 + -1.4 2,218,916 + -2.2 2,218,917 - +1.2 2,218,941 + -0.3 2,218,941 + -0.5 2,218,941 + +0.2 2,218,942 - -1.0 2,218,947 + -0.4 2,218,995 - +0.1 2,219,000 + +1.2 2,219,001 - -0.8 2,219,044 - -2.0 2,219,116 + +0.6 2,219,116 + -1.0 2,219,117 - -0.2 2,219,117 - -0.9 2,219,131 - +0.6 2,219,131 - -0.4 2,219,181 - -1.0 2,219,201 - HGI48_RS10280 0.27 +0.6 2,219,267 + HGI48_RS10280 0.42 +1.2 2,219,267 + +0.6 2,219,267 + HGI48_RS10280 0.42 -0.6 2,219,268 - HGI48_RS10280 0.43 -0.7 2,219,268 - HGI48_RS10280 0.43 -1.0 2,219,350 + HGI48_RS10280 0.62 -1.5 2,219,351 - HGI48_RS10280 0.63 -1.0 2,219,351 - HGI48_RS10280 0.63 +0.6 2,219,351 - HGI48_RS10280 0.63 -0.7 2,219,522 - -0.8 2,219,522 - -0.4 2,219,588 + +0.1 2,219,589 - -2.1 2,219,591 + -0.0 2,219,598 + -0.4 2,219,706 + HGI48_RS10285 0.49 -0.1 2,219,706 + HGI48_RS10285 0.49 +0.0 2,219,707 - HGI48_RS10285 0.49 +0.6 2,219,707 - HGI48_RS10285 0.49 -0.5 2,219,728 - HGI48_RS10285 0.57 -1.4 2,219,817 + HGI48_RS10285 0.89 +0.4 2,219,817 + HGI48_RS10285 0.89 -0.6 2,219,817 + HGI48_RS10285 0.89 +0.2 2,219,817 + HGI48_RS10285 0.89 +0.1 2,219,818 - HGI48_RS10285 0.89 -0.9 2,219,820 + +1.6 2,219,820 + -0.7 2,219,820 + -1.0 2,219,821 - +1.0 2,219,821 - +0.8 2,219,821 - -1.7 2,219,821 - +0.6 2,219,845 + +1.4 2,219,887 + -0.4 2,219,888 - +0.6 2,219,894 + -2.6 2,219,895 - +2.2 2,219,913 + +1.7 2,219,913 + -0.4 2,220,019 + HGI48_RS10290 0.20 +1.9 2,220,019 + HGI48_RS10290 0.20 +0.6 2,220,019 + HGI48_RS10290 0.20 +0.7 2,220,020 - HGI48_RS10290 0.20 -0.1 2,220,060 + HGI48_RS10290 0.26 +0.6 2,220,062 + HGI48_RS10290 0.26 +0.4 2,220,062 + HGI48_RS10290 0.26 +1.0 2,220,063 - HGI48_RS10290 0.26 +0.0 2,220,063 - HGI48_RS10290 0.26 -0.6 2,220,134 + HGI48_RS10290 0.38 -0.7 2,220,134 + HGI48_RS10290 0.38 +0.4 2,220,135 - HGI48_RS10290 0.38 +0.8 2,220,135 - HGI48_RS10290 0.38 +0.4 2,220,150 + HGI48_RS10290 0.40 -2.4 2,220,180 - HGI48_RS10290 0.45 +0.9 2,220,223 + HGI48_RS10290 0.51 -0.1 2,220,275 - HGI48_RS10290 0.60 -0.2 2,220,517 + -1.8 2,220,535 - -0.3 2,220,548 + -1.1 2,220,548 + +1.8 2,220,548 + -0.3 2,220,548 + -0.8 2,220,548 + -1.8 2,220,549 - +0.8 2,220,549 - +0.3 2,220,549 - -0.1 2,220,560 + -1.8 2,220,596 + +0.1 2,220,596 + +0.4 2,220,597 - -1.8 2,220,679 + +0.2 2,220,679 + +0.4 2,220,680 - -0.2 2,220,683 + +2.4 2,220,683 + -1.0 2,220,684 - -1.6 2,220,717 + HGI48_RS10295 0.12 -0.3 2,220,717 + HGI48_RS10295 0.12 +0.1 2,220,718 - HGI48_RS10295 0.12 +0.2 2,220,811 - HGI48_RS10295 0.21 -0.3 2,220,815 - HGI48_RS10295 0.22 -0.2 2,220,890 + HGI48_RS10295 0.29 -1.4 2,220,890 + HGI48_RS10295 0.29 +0.1 2,220,973 + -0.0 2,220,974 - HGI48_RS10295 0.37 -1.3 2,221,090 - HGI48_RS10295 0.49 +0.6 2,221,205 + HGI48_RS10295 0.60 +0.1 2,221,205 + HGI48_RS10295 0.60 -0.2 2,221,206 - HGI48_RS10295 0.60 +0.3 2,221,370 + +1.2 2,221,370 + HGI48_RS10295 0.76 -1.4 2,221,380 - HGI48_RS10295 0.77 -0.5 2,221,450 + +0.6 2,221,451 - HGI48_RS10295 0.84 +0.6 2,221,451 - HGI48_RS10295 0.84 +0.6 2,221,454 + HGI48_RS10295 0.84 +1.6 2,221,454 + HGI48_RS10295 0.84 +0.3 2,221,454 + +0.6 2,221,455 - HGI48_RS10295 0.84 -1.2 2,221,455 - HGI48_RS10295 0.84 -1.4 2,221,455 - HGI48_RS10295 0.84 +0.4 2,221,455 - HGI48_RS10295 0.84 -3.0 2,221,455 - +1.6 2,221,455 - HGI48_RS10295 0.84 +0.4
Or see this region's nucleotide sequence