Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HGI48_RS03235 and HGI48_RS03240 are separated by 25 nucleotides HGI48_RS03240 and HGI48_RS03245 are separated by 74 nucleotides HGI48_RS03245 and HGI48_RS03250 overlap by 1 nucleotides
HGI48_RS03235: HGI48_RS03235 - transcription termination/antitermination protein NusA, at 681,671 to 683,161
_RS03235
HGI48_RS03240: HGI48_RS03240 - translation initiation factor IF-2, at 683,187 to 685,904
_RS03240
HGI48_RS03245: HGI48_RS03245 - 30S ribosome-binding factor RbfA, at 685,979 to 686,380
_RS03245
HGI48_RS03250: HGI48_RS03250 - tRNA pseudouridine(55) synthase TruB, at 686,380 to 687,336
_RS03250
Position (kb) 683
684
685
686 Strain fitness (log2 ratio) -2
-1
0
1
2 at 683.008 kb on + strand, within HGI48_RS03235 at 683.131 kb on + strand at 683.131 kb on + strand at 683.131 kb on + strand at 683.169 kb on + strand at 683.169 kb on + strand at 686.347 kb on + strand at 686.379 kb on - strand at 686.474 kb on + strand at 686.475 kb on - strand at 686.508 kb on + strand, within HGI48_RS03250 at 686.509 kb on - strand, within HGI48_RS03250 at 686.509 kb on - strand, within HGI48_RS03250 at 686.509 kb on - strand, within HGI48_RS03250 at 686.598 kb on + strand, within HGI48_RS03250 at 686.598 kb on + strand at 686.640 kb on + strand, within HGI48_RS03250 at 686.658 kb on + strand, within HGI48_RS03250 at 686.658 kb on + strand at 686.658 kb on + strand, within HGI48_RS03250 at 686.658 kb on + strand, within HGI48_RS03250 at 686.756 kb on - strand at 686.756 kb on - strand, within HGI48_RS03250 at 686.756 kb on - strand, within HGI48_RS03250 at 686.756 kb on - strand, within HGI48_RS03250 at 686.770 kb on + strand, within HGI48_RS03250 at 686.846 kb on - strand, within HGI48_RS03250 at 686.853 kb on - strand, within HGI48_RS03250 at 686.853 kb on - strand, within HGI48_RS03250
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 683,008 + HGI48_RS03235 0.90 -0.5 683,131 + -0.8 683,131 + -1.8 683,131 + -1.2 683,169 + -0.2 683,169 + -1.1 686,347 + -0.8 686,379 - -0.1 686,474 + +0.5 686,475 - +0.7 686,508 + HGI48_RS03250 0.13 +0.2 686,509 - HGI48_RS03250 0.13 -0.2 686,509 - HGI48_RS03250 0.13 +0.5 686,509 - HGI48_RS03250 0.13 -0.9 686,598 + HGI48_RS03250 0.23 +1.8 686,598 + -1.1 686,640 + HGI48_RS03250 0.27 +0.3 686,658 + HGI48_RS03250 0.29 -1.5 686,658 + +0.5 686,658 + HGI48_RS03250 0.29 +0.5 686,658 + HGI48_RS03250 0.29 +1.5 686,756 - +0.5 686,756 - HGI48_RS03250 0.39 +0.1 686,756 - HGI48_RS03250 0.39 -0.1 686,756 - HGI48_RS03250 0.39 +0.5 686,770 + HGI48_RS03250 0.41 -0.4 686,846 - HGI48_RS03250 0.49 -0.6 686,853 - HGI48_RS03250 0.49 +1.4 686,853 - HGI48_RS03250 0.49 +0.8
Or see this region's nucleotide sequence