Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HGI48_RS01020 and HGI48_RS01025 overlap by 4 nucleotides HGI48_RS01025 and HGI48_RS01030 are separated by 26 nucleotides HGI48_RS01030 and HGI48_RS01035 are separated by 683 nucleotides HGI48_RS01035 and HGI48_RS01040 are separated by 16 nucleotides HGI48_RS01040 and HGI48_RS01045 are separated by 135 nucleotides
HGI48_RS01020: HGI48_RS01020 - UDP-N-acetylmuramate dehydrogenase, at 232,181 to 233,218
_RS01020
HGI48_RS01025: HGI48_RS01025 - bifunctional biotin--[acetyl-CoA-carboxylase] ligase/biotin operon repressor BirA, at 233,215 to 234,174
_RS01025
HGI48_RS01030: HGI48_RS01030 - type I pantothenate kinase, at 234,201 to 235,151
_RS01030
HGI48_RS01035: HGI48_RS01035 - tRNA-Thr, at 235,835 to 235,910
_RS01035
HGI48_RS01040: HGI48_RS01040 - tRNA-Tyr, at 235,927 to 236,011
_RS01040
HGI48_RS01045: HGI48_RS01045 - tRNA-Gly, at 236,147 to 236,221
_RS01045
Position (kb) 234
235
236 Strain fitness (log2 ratio) -1
0
1
2 at 233.224 kb on + strand at 233.226 kb on + strand at 234.176 kb on - strand at 235.152 kb on - strand at 235.158 kb on - strand at 235.158 kb on - strand at 235.158 kb on - strand at 235.328 kb on + strand at 235.350 kb on - strand at 235.390 kb on - strand at 235.417 kb on + strand at 235.417 kb on + strand at 235.417 kb on + strand at 235.417 kb on + strand at 235.420 kb on + strand at 235.421 kb on - strand at 235.421 kb on - strand at 235.452 kb on + strand at 235.452 kb on + strand at 235.452 kb on + strand at 235.452 kb on + strand at 235.453 kb on - strand at 235.453 kb on - strand at 235.453 kb on - strand at 235.461 kb on + strand at 235.462 kb on - strand at 235.462 kb on - strand at 235.462 kb on - strand at 235.504 kb on - strand at 235.525 kb on + strand at 235.526 kb on - strand at 235.546 kb on - strand at 235.618 kb on + strand at 235.619 kb on - strand at 235.668 kb on + strand at 235.668 kb on + strand at 235.668 kb on + strand at 235.685 kb on + strand at 235.686 kb on - strand at 235.705 kb on - strand at 235.705 kb on - strand at 236.035 kb on + strand at 236.035 kb on + strand at 236.035 kb on + strand at 236.047 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 233,224 + -1.5 233,226 + -1.2 234,176 - -0.4 235,152 - +0.6 235,158 - -1.6 235,158 - -1.4 235,158 - -0.3 235,328 + +0.6 235,350 - -0.4 235,390 - -0.7 235,417 + -0.3 235,417 + +0.1 235,417 + -0.2 235,417 + +0.1 235,420 + -1.5 235,421 - +1.9 235,421 - +1.0 235,452 + -0.1 235,452 + -1.4 235,452 + -0.9 235,452 + -0.7 235,453 - -0.5 235,453 - -0.0 235,453 - +1.2 235,461 + +0.9 235,462 - -1.7 235,462 - +1.0 235,462 - +0.9 235,504 - +0.4 235,525 + +0.3 235,526 - -1.7 235,546 - -0.2 235,618 + -0.6 235,619 - +0.4 235,668 + -1.0 235,668 + -1.2 235,668 + +0.2 235,685 + -0.1 235,686 - +1.4 235,705 - +0.6 235,705 - -0.6 236,035 + -1.4 236,035 + -0.6 236,035 + -0.4 236,047 + +0.8
Or see this region's nucleotide sequence