Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HGI48_RS14070 and HGI48_RS14075 are separated by 260 nucleotides HGI48_RS14075 and HGI48_RS14080 are separated by 47 nucleotides HGI48_RS14080 and HGI48_RS14085 are separated by 27 nucleotides
HGI48_RS14070: HGI48_RS14070 - 2,3-diaminopropionate biosynthesis protein SbnB, at 3,147,868 to 3,148,875
_RS14070
HGI48_RS14075: HGI48_RS14075 - NAD(P)H-dependent oxidoreductase, at 3,149,136 to 3,149,681
_RS14075
HGI48_RS14080: HGI48_RS14080 - uroporphyrinogen-III C-methyltransferase, at 3,149,729 to 3,150,559
_RS14080
HGI48_RS14085: HGI48_RS14085 - nitrate reductase, at 3,150,587 to 3,153,235
_RS14085
Position (kb)
3149
3150
3151 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3148.740 kb on + strand, within HGI48_RS14070 at 3148.743 kb on + strand, within HGI48_RS14070 at 3148.756 kb on + strand, within HGI48_RS14070 at 3148.841 kb on + strand at 3148.842 kb on - strand at 3148.845 kb on + strand at 3148.846 kb on - strand at 3148.895 kb on + strand at 3148.927 kb on + strand at 3148.977 kb on + strand at 3148.978 kb on - strand at 3148.978 kb on - strand at 3148.978 kb on - strand at 3148.978 kb on - strand at 3148.978 kb on - strand at 3148.999 kb on - strand at 3149.045 kb on + strand at 3149.046 kb on - strand at 3149.046 kb on - strand at 3149.050 kb on + strand at 3149.050 kb on + strand at 3149.051 kb on - strand at 3149.051 kb on - strand at 3149.051 kb on - strand at 3149.156 kb on - strand at 3149.160 kb on - strand at 3149.274 kb on + strand, within HGI48_RS14075 at 3149.274 kb on + strand, within HGI48_RS14075 at 3149.277 kb on - strand, within HGI48_RS14075 at 3149.368 kb on - strand, within HGI48_RS14075 at 3149.368 kb on - strand, within HGI48_RS14075 at 3149.368 kb on - strand, within HGI48_RS14075 at 3149.373 kb on + strand, within HGI48_RS14075 at 3149.374 kb on - strand, within HGI48_RS14075 at 3149.374 kb on - strand, within HGI48_RS14075 at 3149.748 kb on - strand at 3150.000 kb on + strand at 3150.000 kb on + strand, within HGI48_RS14080 at 3150.000 kb on + strand, within HGI48_RS14080 at 3150.001 kb on - strand, within HGI48_RS14080 at 3150.001 kb on - strand, within HGI48_RS14080 at 3150.001 kb on - strand, within HGI48_RS14080 at 3150.079 kb on + strand, within HGI48_RS14080 at 3150.194 kb on + strand, within HGI48_RS14080 at 3150.194 kb on + strand, within HGI48_RS14080 at 3150.195 kb on - strand, within HGI48_RS14080 at 3150.195 kb on - strand, within HGI48_RS14080 at 3150.198 kb on + strand, within HGI48_RS14080 at 3150.198 kb on + strand, within HGI48_RS14080 at 3150.198 kb on + strand at 3150.199 kb on - strand, within HGI48_RS14080 at 3150.199 kb on - strand, within HGI48_RS14080 at 3150.350 kb on - strand, within HGI48_RS14080 at 3150.350 kb on - strand at 3150.379 kb on + strand, within HGI48_RS14080 at 3150.379 kb on + strand, within HGI48_RS14080 at 3150.379 kb on + strand, within HGI48_RS14080 at 3150.380 kb on - strand, within HGI48_RS14080 at 3150.380 kb on - strand, within HGI48_RS14080 at 3150.382 kb on + strand at 3150.449 kb on - strand, within HGI48_RS14080 at 3150.550 kb on + strand at 3150.602 kb on + strand at 3150.603 kb on - strand at 3150.621 kb on + strand at 3150.621 kb on + strand at 3150.622 kb on - strand at 3150.622 kb on - strand at 3150.622 kb on - strand at 3150.906 kb on + strand, within HGI48_RS14085 at 3150.906 kb on + strand, within HGI48_RS14085 at 3150.906 kb on + strand, within HGI48_RS14085 at 3150.907 kb on - strand, within HGI48_RS14085 at 3150.907 kb on - strand, within HGI48_RS14085 at 3150.907 kb on - strand, within HGI48_RS14085 at 3150.907 kb on - strand, within HGI48_RS14085 at 3150.907 kb on - strand, within HGI48_RS14085 at 3150.907 kb on - strand, within HGI48_RS14085 at 3150.907 kb on - strand, within HGI48_RS14085 at 3150.907 kb on - strand, within HGI48_RS14085 at 3151.186 kb on + strand, within HGI48_RS14085 at 3151.186 kb on + strand, within HGI48_RS14085 at 3151.186 kb on + strand, within HGI48_RS14085 at 3151.187 kb on - strand, within HGI48_RS14085 at 3151.255 kb on + strand, within HGI48_RS14085 at 3151.416 kb on + strand, within HGI48_RS14085 at 3151.416 kb on + strand at 3151.417 kb on - strand, within HGI48_RS14085
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 3,148,740 + HGI48_RS14070 0.87 +0.4 3,148,743 + HGI48_RS14070 0.87 +0.7 3,148,756 + HGI48_RS14070 0.88 +0.2 3,148,841 + -0.4 3,148,842 - -2.2 3,148,845 + +0.1 3,148,846 - -0.0 3,148,895 + -0.9 3,148,927 + +0.8 3,148,977 + -0.0 3,148,978 - -1.2 3,148,978 - +0.9 3,148,978 - -0.4 3,148,978 - +0.3 3,148,978 - -0.4 3,148,999 - -0.0 3,149,045 + -0.1 3,149,046 - -0.4 3,149,046 - -0.9 3,149,050 + -0.3 3,149,050 + -0.1 3,149,051 - +0.6 3,149,051 - -1.0 3,149,051 - -0.4 3,149,156 - -1.2 3,149,160 - +0.2 3,149,274 + HGI48_RS14075 0.25 -0.3 3,149,274 + HGI48_RS14075 0.25 -2.7 3,149,277 - HGI48_RS14075 0.26 -0.2 3,149,368 - HGI48_RS14075 0.42 -0.4 3,149,368 - HGI48_RS14075 0.42 -1.0 3,149,368 - HGI48_RS14075 0.42 +0.9 3,149,373 + HGI48_RS14075 0.43 +0.8 3,149,374 - HGI48_RS14075 0.44 +0.4 3,149,374 - HGI48_RS14075 0.44 -0.2 3,149,748 - -1.6 3,150,000 + +0.6 3,150,000 + HGI48_RS14080 0.33 -0.3 3,150,000 + HGI48_RS14080 0.33 -0.4 3,150,001 - HGI48_RS14080 0.33 +0.3 3,150,001 - HGI48_RS14080 0.33 +1.1 3,150,001 - HGI48_RS14080 0.33 -2.0 3,150,079 + HGI48_RS14080 0.42 -1.4 3,150,194 + HGI48_RS14080 0.56 -0.9 3,150,194 + HGI48_RS14080 0.56 +1.2 3,150,195 - HGI48_RS14080 0.56 -1.4 3,150,195 - HGI48_RS14080 0.56 +1.4 3,150,198 + HGI48_RS14080 0.56 -0.0 3,150,198 + HGI48_RS14080 0.56 -0.9 3,150,198 + -1.0 3,150,199 - HGI48_RS14080 0.57 +0.4 3,150,199 - HGI48_RS14080 0.57 -0.1 3,150,350 - HGI48_RS14080 0.75 +0.3 3,150,350 - +0.6 3,150,379 + HGI48_RS14080 0.78 -0.4 3,150,379 + HGI48_RS14080 0.78 +0.5 3,150,379 + HGI48_RS14080 0.78 +0.0 3,150,380 - HGI48_RS14080 0.78 -0.7 3,150,380 - HGI48_RS14080 0.78 +0.3 3,150,382 + +2.3 3,150,449 - HGI48_RS14080 0.87 -0.0 3,150,550 + +1.3 3,150,602 + -0.4 3,150,603 - -0.4 3,150,621 + +0.6 3,150,621 + -1.6 3,150,622 - +0.3 3,150,622 - +0.3 3,150,622 - +0.3 3,150,906 + HGI48_RS14085 0.12 -0.4 3,150,906 + HGI48_RS14085 0.12 -0.6 3,150,906 + HGI48_RS14085 0.12 -2.3 3,150,907 - HGI48_RS14085 0.12 +1.4 3,150,907 - HGI48_RS14085 0.12 -1.0 3,150,907 - HGI48_RS14085 0.12 -0.4 3,150,907 - HGI48_RS14085 0.12 +0.1 3,150,907 - HGI48_RS14085 0.12 -0.0 3,150,907 - HGI48_RS14085 0.12 +0.1 3,150,907 - HGI48_RS14085 0.12 -2.0 3,150,907 - HGI48_RS14085 0.12 +0.5 3,151,186 + HGI48_RS14085 0.23 -0.4 3,151,186 + HGI48_RS14085 0.23 -0.6 3,151,186 + HGI48_RS14085 0.23 +0.4 3,151,187 - HGI48_RS14085 0.23 +1.1 3,151,255 + HGI48_RS14085 0.25 +0.8 3,151,416 + HGI48_RS14085 0.31 -1.7 3,151,416 + +1.0 3,151,417 - HGI48_RS14085 0.31 -1.0
Or see this region's nucleotide sequence