Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HGI48_RS13115 and HGI48_RS13120 are separated by 40 nucleotides HGI48_RS13120 and HGI48_RS13125 overlap by 23 nucleotides HGI48_RS13125 and HGI48_RS13130 are separated by 21 nucleotides HGI48_RS13130 and HGI48_RS13135 are separated by 7 nucleotides HGI48_RS13135 and HGI48_RS13140 are separated by 113 nucleotides
HGI48_RS13115: HGI48_RS13115 - beta-N-acetylhexosaminidase, at 2,874,430 to 2,875,455
_RS13115
HGI48_RS13120: HGI48_RS13120 - phosphotransferase, at 2,875,496 to 2,876,350
_RS13120
HGI48_RS13125: HGI48_RS13125 - penicillin-binding protein activator LpoB, at 2,876,328 to 2,876,921
_RS13125
HGI48_RS13130: HGI48_RS13130 - DUF1425 domain-containing protein, at 2,876,943 to 2,877,326
_RS13130
HGI48_RS13135: HGI48_RS13135 - purine nucleoside phosphoramidase, at 2,877,334 to 2,877,684
_RS13135
HGI48_RS13140: HGI48_RS13140 - malate/lactate/ureidoglycolate dehydrogenase, at 2,877,798 to 2,878,859
_RS13140
Position (kb)
2876
2877 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2875.354 kb on - strand at 2875.404 kb on + strand at 2875.438 kb on + strand at 2875.438 kb on + strand at 2875.438 kb on + strand at 2875.439 kb on - strand at 2875.439 kb on - strand at 2875.439 kb on - strand at 2875.439 kb on - strand at 2875.439 kb on - strand at 2875.470 kb on + strand at 2875.471 kb on - strand at 2875.471 kb on - strand at 2875.536 kb on + strand at 2875.590 kb on + strand at 2875.590 kb on + strand at 2875.666 kb on - strand, within HGI48_RS13120 at 2875.666 kb on - strand, within HGI48_RS13120 at 2875.918 kb on + strand, within HGI48_RS13120 at 2875.918 kb on + strand at 2875.968 kb on + strand at 2876.267 kb on + strand at 2876.267 kb on + strand at 2876.267 kb on + strand at 2876.267 kb on + strand at 2876.438 kb on - strand, within HGI48_RS13125 at 2877.009 kb on - strand, within HGI48_RS13130 at 2877.009 kb on - strand, within HGI48_RS13130 at 2877.079 kb on + strand, within HGI48_RS13130 at 2877.079 kb on + strand, within HGI48_RS13130 at 2877.079 kb on + strand, within HGI48_RS13130 at 2877.080 kb on - strand at 2877.085 kb on + strand, within HGI48_RS13130 at 2877.122 kb on + strand at 2877.122 kb on + strand, within HGI48_RS13130 at 2877.122 kb on + strand, within HGI48_RS13130 at 2877.123 kb on - strand, within HGI48_RS13130 at 2877.123 kb on - strand, within HGI48_RS13130 at 2877.239 kb on + strand, within HGI48_RS13130 at 2877.318 kb on + strand at 2877.327 kb on - strand at 2877.381 kb on + strand, within HGI48_RS13135 at 2877.381 kb on + strand, within HGI48_RS13135 at 2877.381 kb on + strand at 2877.381 kb on + strand at 2877.382 kb on - strand, within HGI48_RS13135 at 2877.382 kb on - strand, within HGI48_RS13135 at 2877.392 kb on + strand, within HGI48_RS13135 at 2877.392 kb on + strand, within HGI48_RS13135 at 2877.393 kb on - strand, within HGI48_RS13135 at 2877.393 kb on - strand, within HGI48_RS13135 at 2877.393 kb on - strand, within HGI48_RS13135 at 2877.393 kb on - strand, within HGI48_RS13135 at 2877.393 kb on - strand, within HGI48_RS13135 at 2877.567 kb on + strand, within HGI48_RS13135 at 2877.568 kb on - strand, within HGI48_RS13135 at 2877.568 kb on - strand, within HGI48_RS13135 at 2877.631 kb on - strand, within HGI48_RS13135 at 2877.684 kb on + strand at 2877.700 kb on - strand at 2877.734 kb on - strand at 2877.734 kb on - strand at 2877.742 kb on + strand at 2877.743 kb on - strand at 2877.782 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 2,875,354 - -1.6 2,875,404 + -0.5 2,875,438 + +0.2 2,875,438 + -1.3 2,875,438 + -0.9 2,875,439 - -0.4 2,875,439 - -0.8 2,875,439 - -1.5 2,875,439 - +0.0 2,875,439 - +0.1 2,875,470 + -0.6 2,875,471 - -0.5 2,875,471 - -0.1 2,875,536 + -1.1 2,875,590 + +1.3 2,875,590 + +2.1 2,875,666 - HGI48_RS13120 0.20 -1.1 2,875,666 - HGI48_RS13120 0.20 -0.3 2,875,918 + HGI48_RS13120 0.49 -0.2 2,875,918 + -0.1 2,875,968 + +0.5 2,876,267 + -0.1 2,876,267 + +0.1 2,876,267 + -0.8 2,876,267 + +0.2 2,876,438 - HGI48_RS13125 0.19 -2.1 2,877,009 - HGI48_RS13130 0.17 +0.3 2,877,009 - HGI48_RS13130 0.17 -0.1 2,877,079 + HGI48_RS13130 0.35 -0.6 2,877,079 + HGI48_RS13130 0.35 -0.8 2,877,079 + HGI48_RS13130 0.35 -0.5 2,877,080 - -0.1 2,877,085 + HGI48_RS13130 0.37 +0.2 2,877,122 + -0.5 2,877,122 + HGI48_RS13130 0.47 -1.0 2,877,122 + HGI48_RS13130 0.47 +0.1 2,877,123 - HGI48_RS13130 0.47 +0.2 2,877,123 - HGI48_RS13130 0.47 -0.5 2,877,239 + HGI48_RS13130 0.77 -0.1 2,877,318 + -0.9 2,877,327 - -2.9 2,877,381 + HGI48_RS13135 0.13 +0.5 2,877,381 + HGI48_RS13135 0.13 -0.1 2,877,381 + +0.9 2,877,381 + -0.1 2,877,382 - HGI48_RS13135 0.14 -0.5 2,877,382 - HGI48_RS13135 0.14 -0.2 2,877,392 + HGI48_RS13135 0.17 -1.1 2,877,392 + HGI48_RS13135 0.17 +0.0 2,877,393 - HGI48_RS13135 0.17 +0.8 2,877,393 - HGI48_RS13135 0.17 -1.4 2,877,393 - HGI48_RS13135 0.17 +0.2 2,877,393 - HGI48_RS13135 0.17 -0.3 2,877,393 - HGI48_RS13135 0.17 -2.1 2,877,567 + HGI48_RS13135 0.66 -1.1 2,877,568 - HGI48_RS13135 0.67 +0.5 2,877,568 - HGI48_RS13135 0.67 -0.0 2,877,631 - HGI48_RS13135 0.85 -0.1 2,877,684 + -0.1 2,877,700 - -0.5 2,877,734 - +1.7 2,877,734 - +0.1 2,877,742 + -1.1 2,877,743 - -0.8 2,877,782 - -0.8
Or see this region's nucleotide sequence