Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HGI48_RS03215 and HGI48_RS03220 are separated by 54 nucleotides HGI48_RS03220 and HGI48_RS03225 are separated by 59 nucleotides HGI48_RS03225 and HGI48_RS03230 are separated by 218 nucleotides HGI48_RS03230 and HGI48_RS03235 are separated by 21 nucleotides
HGI48_RS03215: HGI48_RS03215 - preprotein translocase subunit SecG, at 680,366 to 680,701
_RS03215
HGI48_RS03220: HGI48_RS03220 - tRNA-Leu, at 680,756 to 680,842
_RS03220
HGI48_RS03225: HGI48_RS03225 - tRNA-Met, at 680,902 to 680,978
_RS03225
HGI48_RS03230: HGI48_RS03230 - ribosome maturation factor RimP, at 681,197 to 681,649
_RS03230
HGI48_RS03235: HGI48_RS03235 - transcription termination/antitermination protein NusA, at 681,671 to 683,161
_RS03235
Position (kb) 681
682 Strain fitness (log2 ratio) -2
-1
0
1 at 680.247 kb on - strand at 680.423 kb on - strand, within HGI48_RS03215 at 680.695 kb on + strand at 680.695 kb on + strand at 680.740 kb on + strand at 680.740 kb on + strand at 680.848 kb on - strand at 680.849 kb on + strand at 680.850 kb on - strand at 680.853 kb on + strand at 680.853 kb on + strand at 680.854 kb on - strand at 680.854 kb on - strand at 680.981 kb on + strand at 680.981 kb on + strand at 681.009 kb on + strand at 681.016 kb on + strand at 681.067 kb on + strand at 681.067 kb on + strand at 681.067 kb on + strand at 681.067 kb on + strand at 681.071 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 680,247 - -0.3 680,423 - HGI48_RS03215 0.17 -0.4 680,695 + -1.1 680,695 + -1.1 680,740 + -1.9 680,740 + +1.0 680,848 - -0.3 680,849 + -1.1 680,850 - -2.6 680,853 + -0.5 680,853 + -0.1 680,854 - -2.6 680,854 - -0.7 680,981 + -0.5 680,981 + -0.7 681,009 + -1.1 681,016 + -1.1 681,067 + -0.3 681,067 + -1.6 681,067 + -0.3 681,067 + +0.2 681,071 + -0.1
Or see this region's nucleotide sequence