Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HGI48_RS03200 and HGI48_RS03205 are separated by 105 nucleotides HGI48_RS03205 and HGI48_RS03210 are separated by 8 nucleotides HGI48_RS03210 and HGI48_RS03215 are separated by 298 nucleotides HGI48_RS03215 and HGI48_RS03220 are separated by 54 nucleotides HGI48_RS03220 and HGI48_RS03225 are separated by 59 nucleotides
HGI48_RS03200: HGI48_RS03200 - ATP-dependent zinc metalloprotease FtsH, at 675,830 to 677,782
_RS03200
HGI48_RS03205: HGI48_RS03205 - dihydropteroate synthase, at 677,888 to 678,721
_RS03205
HGI48_RS03210: HGI48_RS03210 - phosphoglucosamine mutase, at 678,730 to 680,067
_RS03210
HGI48_RS03215: HGI48_RS03215 - preprotein translocase subunit SecG, at 680,366 to 680,701
_RS03215
HGI48_RS03220: HGI48_RS03220 - tRNA-Leu, at 680,756 to 680,842
_RS03220
HGI48_RS03225: HGI48_RS03225 - tRNA-Met, at 680,902 to 680,978
_RS03225
Position (kb)
678
679
680
681 Strain fitness (log2 ratio)
-2
-1
0
1 at 677.734 kb on + strand at 677.734 kb on + strand at 677.735 kb on - strand at 677.752 kb on + strand at 677.752 kb on + strand at 677.753 kb on - strand at 677.753 kb on - strand at 677.753 kb on - strand at 677.753 kb on - strand at 677.753 kb on - strand at 677.753 kb on - strand at 677.753 kb on - strand at 677.780 kb on + strand at 677.780 kb on + strand at 677.780 kb on + strand at 677.780 kb on + strand at 677.780 kb on + strand at 677.780 kb on + strand at 677.780 kb on + strand at 677.780 kb on + strand at 677.780 kb on + strand at 677.781 kb on - strand at 677.781 kb on - strand at 677.781 kb on - strand at 677.781 kb on - strand at 677.781 kb on - strand at 677.781 kb on - strand at 677.859 kb on - strand at 677.875 kb on + strand at 677.875 kb on + strand at 678.348 kb on - strand, within HGI48_RS03205 at 678.392 kb on - strand, within HGI48_RS03205 at 678.392 kb on - strand, within HGI48_RS03205 at 678.468 kb on + strand, within HGI48_RS03205 at 678.475 kb on + strand, within HGI48_RS03205 at 678.476 kb on - strand, within HGI48_RS03205 at 678.684 kb on + strand at 678.684 kb on + strand at 678.684 kb on + strand at 678.685 kb on - strand at 678.685 kb on - strand at 678.685 kb on - strand at 678.685 kb on - strand at 678.685 kb on - strand at 678.685 kb on - strand at 678.719 kb on + strand at 678.729 kb on + strand at 680.077 kb on + strand at 680.078 kb on - strand at 680.078 kb on - strand at 680.143 kb on - strand at 680.143 kb on - strand at 680.143 kb on - strand at 680.247 kb on - strand at 680.423 kb on - strand, within HGI48_RS03215 at 680.695 kb on + strand at 680.695 kb on + strand at 680.740 kb on + strand at 680.740 kb on + strand at 680.848 kb on - strand at 680.849 kb on + strand at 680.850 kb on - strand at 680.853 kb on + strand at 680.853 kb on + strand at 680.854 kb on - strand at 680.854 kb on - strand at 680.981 kb on + strand at 680.981 kb on + strand at 681.009 kb on + strand at 681.016 kb on + strand at 681.067 kb on + strand at 681.067 kb on + strand at 681.067 kb on + strand at 681.067 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 677,734 + -1.7 677,734 + +0.3 677,735 - -0.9 677,752 + -0.1 677,752 + -1.4 677,753 - -2.3 677,753 - -0.7 677,753 - -1.8 677,753 - -2.1 677,753 - +1.5 677,753 - -1.4 677,753 - +0.3 677,780 + -1.1 677,780 + -0.3 677,780 + +0.0 677,780 + -1.1 677,780 + +0.3 677,780 + -0.7 677,780 + +0.4 677,780 + -1.1 677,780 + +0.2 677,781 - +1.1 677,781 - -0.1 677,781 - -1.5 677,781 - -0.4 677,781 - +0.1 677,781 - +1.3 677,859 - +0.3 677,875 + +0.9 677,875 + +0.3 678,348 - HGI48_RS03205 0.55 +0.6 678,392 - HGI48_RS03205 0.60 -0.5 678,392 - HGI48_RS03205 0.60 +0.5 678,468 + HGI48_RS03205 0.70 +0.9 678,475 + HGI48_RS03205 0.70 -0.1 678,476 - HGI48_RS03205 0.71 -1.1 678,684 + -0.5 678,684 + +0.2 678,684 + -1.3 678,685 - -2.4 678,685 - -1.0 678,685 - -2.4 678,685 - -0.5 678,685 - -1.1 678,685 - -0.1 678,719 + +0.2 678,729 + -2.1 680,077 + -0.4 680,078 - -0.2 680,078 - -0.7 680,143 - -0.6 680,143 - -1.7 680,143 - -1.3 680,247 - -0.3 680,423 - HGI48_RS03215 0.17 -0.4 680,695 + -1.1 680,695 + -1.1 680,740 + -1.9 680,740 + +1.0 680,848 - -0.3 680,849 + -1.1 680,850 - -2.6 680,853 + -0.5 680,853 + -0.1 680,854 - -2.6 680,854 - -0.7 680,981 + -0.5 680,981 + -0.7 681,009 + -1.1 681,016 + -1.1 681,067 + -0.3 681,067 + -1.6 681,067 + -0.3 681,067 + +0.2
Or see this region's nucleotide sequence