Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HGI48_RS01350 and HGI48_RS01355 are separated by 103 nucleotides HGI48_RS01355 and HGI48_RS01360 overlap by 4 nucleotides HGI48_RS01360 and HGI48_RS01365 are separated by 24 nucleotides
HGI48_RS01350: HGI48_RS01350 - rod shape-determining protein MreB, at 300,253 to 301,296
_RS01350
HGI48_RS01355: HGI48_RS01355 - rod shape-determining protein MreC, at 301,400 to 302,512
_RS01355
HGI48_RS01360: HGI48_RS01360 - rod shape-determining protein MreD, at 302,509 to 302,997
_RS01360
HGI48_RS01365: HGI48_RS01365 - septum formation inhibitor Maf, at 303,022 to 303,615
_RS01365
Position (kb) 301
302
303 Strain fitness (log2 ratio) -2
-1
0
1
2 at 301.295 kb on - strand at 301.347 kb on + strand at 301.347 kb on + strand at 301.347 kb on + strand at 301.348 kb on - strand at 301.348 kb on - strand at 301.348 kb on - strand at 301.348 kb on - strand at 301.348 kb on - strand at 301.348 kb on - strand at 301.376 kb on + strand at 301.377 kb on - strand at 301.389 kb on + strand at 302.353 kb on + strand, within HGI48_RS01355 at 302.353 kb on + strand, within HGI48_RS01355 at 302.428 kb on + strand at 302.514 kb on + strand at 302.514 kb on + strand at 302.514 kb on + strand at 302.514 kb on + strand at 302.514 kb on + strand at 302.514 kb on + strand at 302.996 kb on - strand at 303.009 kb on + strand at 303.034 kb on + strand at 303.034 kb on + strand at 303.034 kb on + strand at 303.034 kb on + strand at 303.035 kb on - strand at 303.151 kb on + strand, within HGI48_RS01365 at 303.151 kb on + strand, within HGI48_RS01365 at 303.151 kb on + strand, within HGI48_RS01365 at 303.151 kb on + strand at 303.151 kb on + strand, within HGI48_RS01365 at 303.151 kb on + strand, within HGI48_RS01365 at 303.151 kb on + strand, within HGI48_RS01365 at 303.152 kb on - strand, within HGI48_RS01365 at 303.152 kb on - strand, within HGI48_RS01365 at 303.152 kb on - strand, within HGI48_RS01365 at 303.294 kb on + strand, within HGI48_RS01365 at 303.392 kb on + strand, within HGI48_RS01365 at 303.423 kb on + strand, within HGI48_RS01365 at 303.423 kb on + strand, within HGI48_RS01365 at 303.424 kb on - strand, within HGI48_RS01365 at 303.424 kb on - strand, within HGI48_RS01365 at 303.430 kb on + strand, within HGI48_RS01365 at 303.430 kb on + strand, within HGI48_RS01365 at 303.430 kb on + strand at 303.430 kb on + strand, within HGI48_RS01365 at 303.430 kb on + strand, within HGI48_RS01365 at 303.430 kb on + strand, within HGI48_RS01365 at 303.430 kb on + strand, within HGI48_RS01365 at 303.430 kb on + strand, within HGI48_RS01365 at 303.430 kb on + strand, within HGI48_RS01365 at 303.430 kb on + strand, within HGI48_RS01365 at 303.430 kb on + strand, within HGI48_RS01365 at 303.430 kb on + strand, within HGI48_RS01365 at 303.431 kb on - strand, within HGI48_RS01365 at 303.431 kb on - strand, within HGI48_RS01365 at 303.431 kb on - strand, within HGI48_RS01365
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 301,295 - -1.6 301,347 + +0.0 301,347 + -1.2 301,347 + +1.3 301,348 - +0.3 301,348 - -0.1 301,348 - -0.8 301,348 - -0.8 301,348 - +0.2 301,348 - -1.0 301,376 + +0.0 301,377 - +1.7 301,389 + -0.2 302,353 + HGI48_RS01355 0.86 +0.3 302,353 + HGI48_RS01355 0.86 -0.8 302,428 + +0.2 302,514 + -1.2 302,514 + -2.2 302,514 + +1.4 302,514 + -0.6 302,514 + +0.7 302,514 + -1.7 302,996 - -1.0 303,009 + -1.6 303,034 + +1.0 303,034 + -0.4 303,034 + -1.0 303,034 + -0.8 303,035 - -0.3 303,151 + HGI48_RS01365 0.22 +0.2 303,151 + HGI48_RS01365 0.22 -0.2 303,151 + HGI48_RS01365 0.22 -0.9 303,151 + +0.0 303,151 + HGI48_RS01365 0.22 -0.3 303,151 + HGI48_RS01365 0.22 -0.3 303,151 + HGI48_RS01365 0.22 +1.0 303,152 - HGI48_RS01365 0.22 -0.5 303,152 - HGI48_RS01365 0.22 +0.0 303,152 - HGI48_RS01365 0.22 +0.0 303,294 + HGI48_RS01365 0.46 +1.5 303,392 + HGI48_RS01365 0.62 -0.6 303,423 + HGI48_RS01365 0.68 -0.7 303,423 + HGI48_RS01365 0.68 -1.6 303,424 - HGI48_RS01365 0.68 -2.0 303,424 - HGI48_RS01365 0.68 +0.0 303,430 + HGI48_RS01365 0.69 -1.0 303,430 + HGI48_RS01365 0.69 +0.9 303,430 + +2.0 303,430 + HGI48_RS01365 0.69 +1.3 303,430 + HGI48_RS01365 0.69 +0.7 303,430 + HGI48_RS01365 0.69 -0.4 303,430 + HGI48_RS01365 0.69 +0.6 303,430 + HGI48_RS01365 0.69 -0.2 303,430 + HGI48_RS01365 0.69 +1.9 303,430 + HGI48_RS01365 0.69 +0.2 303,430 + HGI48_RS01365 0.69 -1.0 303,430 + HGI48_RS01365 0.69 +0.7 303,431 - HGI48_RS01365 0.69 +0.4 303,431 - HGI48_RS01365 0.69 +0.3 303,431 - HGI48_RS01365 0.69 +0.3
Or see this region's nucleotide sequence