Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HGI48_RS01315 and HGI48_RS01320 are separated by 11 nucleotides HGI48_RS01320 and HGI48_RS01325 are separated by 35 nucleotides HGI48_RS01325 and HGI48_RS01330 are separated by 238 nucleotides
HGI48_RS01315: HGI48_RS01315 - acetyl-CoA carboxylase biotin carboxylase subunit, at 292,447 to 293,790
_RS01315
HGI48_RS01320: HGI48_RS01320 - acetyl-CoA carboxylase biotin carboxyl carrier protein, at 293,802 to 294,263
_RS01320
HGI48_RS01325: HGI48_RS01325 - type II 3-dehydroquinate dehydratase, at 294,299 to 294,751
_RS01325
HGI48_RS01330: HGI48_RS01330 - protein-methionine-sulfoxide reductase heme-binding subunit MsrQ, at 294,990 to 295,589
_RS01330
Position (kb) 293
294
295 Strain fitness (log2 ratio) -2
-1
0
1
2 at 294.864 kb on + strand at 294.865 kb on - strand at 294.865 kb on - strand at 294.865 kb on - strand at 294.867 kb on - strand at 294.867 kb on - strand at 294.867 kb on - strand at 294.867 kb on - strand at 294.905 kb on - strand at 295.054 kb on + strand at 295.055 kb on - strand at 295.083 kb on + strand, within HGI48_RS01330 at 295.097 kb on - strand at 295.126 kb on + strand, within HGI48_RS01330 at 295.127 kb on - strand at 295.136 kb on + strand, within HGI48_RS01330 at 295.137 kb on - strand, within HGI48_RS01330 at 295.209 kb on + strand, within HGI48_RS01330
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 294,864 + +0.4 294,865 - -2.5 294,865 - +1.0 294,865 - +0.9 294,867 - -0.8 294,867 - +1.0 294,867 - +1.3 294,867 - +0.5 294,905 - +1.3 295,054 + +2.2 295,055 - +1.0 295,083 + HGI48_RS01330 0.15 -1.2 295,097 - +1.0 295,126 + HGI48_RS01330 0.23 -2.3 295,127 - +2.3 295,136 + HGI48_RS01330 0.24 -0.4 295,137 - HGI48_RS01330 0.24 +0.8 295,209 + HGI48_RS01330 0.36 -0.5
Or see this region's nucleotide sequence