Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HGI48_RS00385 and HGI48_RS00390 are separated by 13 nucleotides HGI48_RS00390 and HGI48_RS00395 overlap by 11 nucleotides HGI48_RS00395 and HGI48_RS00400 are separated by 111 nucleotides HGI48_RS00400 and HGI48_RS00405 overlap by 14 nucleotides
HGI48_RS00385: HGI48_RS00385 - 3-oxoacyl-ACP synthase, at 99,773 to 100,705
_RS00385
HGI48_RS00390: HGI48_RS00390 - acyl carrier protein, at 100,719 to 100,985
_RS00390
HGI48_RS00395: HGI48_RS00395 - VfmB protein, at 100,975 to 101,586
_RS00395
HGI48_RS00400: HGI48_RS00400 - multidrug transporter, at 101,698 to 101,883
_RS00400
HGI48_RS00405: HGI48_RS00405 - alpha/beta hydrolase, at 101,870 to 102,958
_RS00405
Position (kb) 101
102 Strain fitness (log2 ratio) -2
-1
0
1
2 at 100.699 kb on + strand at 100.700 kb on - strand at 100.700 kb on - strand at 100.703 kb on + strand at 100.703 kb on + strand at 100.703 kb on + strand at 100.703 kb on + strand at 100.703 kb on + strand at 100.703 kb on + strand at 100.704 kb on - strand at 100.704 kb on - strand at 100.704 kb on - strand at 100.704 kb on - strand at 100.704 kb on - strand at 100.704 kb on - strand at 100.704 kb on - strand at 100.724 kb on + strand at 100.724 kb on + strand at 100.724 kb on + strand at 100.724 kb on + strand at 100.724 kb on + strand at 100.724 kb on + strand at 100.724 kb on + strand at 100.724 kb on + strand at 100.724 kb on + strand at 100.724 kb on + strand at 100.725 kb on - strand at 100.725 kb on - strand at 100.730 kb on - strand at 100.730 kb on - strand at 100.732 kb on + strand at 100.733 kb on - strand at 100.733 kb on - strand at 100.866 kb on - strand, within HGI48_RS00390 at 100.866 kb on - strand, within HGI48_RS00390 at 101.077 kb on + strand, within HGI48_RS00395 at 101.077 kb on + strand at 101.077 kb on + strand at 101.077 kb on + strand, within HGI48_RS00395 at 101.077 kb on + strand, within HGI48_RS00395 at 101.077 kb on + strand, within HGI48_RS00395 at 101.078 kb on - strand, within HGI48_RS00395 at 101.078 kb on - strand, within HGI48_RS00395 at 101.395 kb on + strand, within HGI48_RS00395 at 101.461 kb on - strand, within HGI48_RS00395 at 101.461 kb on - strand, within HGI48_RS00395 at 101.511 kb on + strand, within HGI48_RS00395 at 101.511 kb on + strand, within HGI48_RS00395 at 101.527 kb on + strand at 101.527 kb on + strand at 101.528 kb on - strand at 101.528 kb on - strand at 101.546 kb on - strand at 101.546 kb on - strand at 101.546 kb on - strand at 101.589 kb on - strand at 101.812 kb on + strand, within HGI48_RS00400 at 101.837 kb on - strand, within HGI48_RS00400 at 101.879 kb on + strand at 101.879 kb on + strand at 101.879 kb on + strand at 101.879 kb on + strand at 101.879 kb on + strand at 101.879 kb on + strand at 101.879 kb on + strand at 101.879 kb on + strand at 101.879 kb on + strand at 101.880 kb on - strand at 101.880 kb on - strand at 101.880 kb on - strand at 101.881 kb on + strand at 101.881 kb on + strand at 101.882 kb on - strand at 101.882 kb on - strand at 101.882 kb on - strand at 101.924 kb on + strand at 101.924 kb on + strand at 101.930 kb on + strand at 101.930 kb on + strand at 101.930 kb on + strand at 101.930 kb on + strand at 101.930 kb on + strand at 101.930 kb on + strand at 101.930 kb on + strand at 101.931 kb on - strand at 101.931 kb on - strand at 101.931 kb on - strand at 101.931 kb on - strand at 101.931 kb on - strand at 101.931 kb on - strand at 101.931 kb on - strand at 101.931 kb on - strand at 101.934 kb on - strand at 101.978 kb on - strand at 101.978 kb on - strand at 101.978 kb on - strand at 102.005 kb on + strand at 102.005 kb on + strand, within HGI48_RS00405 at 102.005 kb on + strand, within HGI48_RS00405 at 102.005 kb on + strand, within HGI48_RS00405 at 102.006 kb on - strand, within HGI48_RS00405 at 102.090 kb on + strand, within HGI48_RS00405 at 102.090 kb on + strand, within HGI48_RS00405 at 102.090 kb on + strand, within HGI48_RS00405 at 102.090 kb on + strand, within HGI48_RS00405 at 102.090 kb on + strand, within HGI48_RS00405 at 102.090 kb on + strand, within HGI48_RS00405 at 102.091 kb on - strand at 102.091 kb on - strand at 102.091 kb on - strand, within HGI48_RS00405 at 102.091 kb on - strand, within HGI48_RS00405 at 102.091 kb on - strand, within HGI48_RS00405 at 102.091 kb on - strand, within HGI48_RS00405 at 102.091 kb on - strand, within HGI48_RS00405 at 102.092 kb on + strand, within HGI48_RS00405 at 102.092 kb on + strand at 102.092 kb on + strand at 102.092 kb on + strand, within HGI48_RS00405 at 102.092 kb on + strand, within HGI48_RS00405 at 102.092 kb on + strand, within HGI48_RS00405 at 102.093 kb on - strand, within HGI48_RS00405 at 102.093 kb on - strand, within HGI48_RS00405 at 102.113 kb on + strand at 102.113 kb on + strand, within HGI48_RS00405 at 102.113 kb on + strand, within HGI48_RS00405 at 102.113 kb on + strand, within HGI48_RS00405 at 102.113 kb on + strand, within HGI48_RS00405 at 102.114 kb on - strand, within HGI48_RS00405 at 102.115 kb on + strand, within HGI48_RS00405 at 102.115 kb on + strand, within HGI48_RS00405 at 102.115 kb on + strand, within HGI48_RS00405 at 102.115 kb on + strand at 102.115 kb on + strand at 102.115 kb on + strand, within HGI48_RS00405 at 102.115 kb on + strand, within HGI48_RS00405 at 102.116 kb on - strand, within HGI48_RS00405 at 102.116 kb on - strand, within HGI48_RS00405 at 102.116 kb on - strand, within HGI48_RS00405 at 102.116 kb on - strand, within HGI48_RS00405 at 102.116 kb on - strand, within HGI48_RS00405 at 102.116 kb on - strand, within HGI48_RS00405 at 102.122 kb on + strand at 102.122 kb on + strand at 102.122 kb on + strand, within HGI48_RS00405 at 102.122 kb on + strand, within HGI48_RS00405 at 102.123 kb on - strand, within HGI48_RS00405 at 102.123 kb on - strand, within HGI48_RS00405 at 102.123 kb on - strand, within HGI48_RS00405 at 102.124 kb on + strand, within HGI48_RS00405 at 102.124 kb on + strand, within HGI48_RS00405 at 102.125 kb on - strand, within HGI48_RS00405 at 102.125 kb on - strand, within HGI48_RS00405 at 102.125 kb on - strand, within HGI48_RS00405 at 102.125 kb on - strand at 102.125 kb on - strand, within HGI48_RS00405 at 102.125 kb on - strand at 102.194 kb on + strand at 102.194 kb on + strand, within HGI48_RS00405 at 102.194 kb on + strand at 102.194 kb on + strand, within HGI48_RS00405 at 102.194 kb on + strand, within HGI48_RS00405 at 102.194 kb on + strand, within HGI48_RS00405 at 102.195 kb on - strand at 102.195 kb on - strand, within HGI48_RS00405 at 102.195 kb on - strand, within HGI48_RS00405 at 102.195 kb on - strand, within HGI48_RS00405 at 102.195 kb on - strand, within HGI48_RS00405 at 102.195 kb on - strand, within HGI48_RS00405 at 102.195 kb on - strand, within HGI48_RS00405 at 102.277 kb on + strand, within HGI48_RS00405 at 102.278 kb on - strand, within HGI48_RS00405 at 102.278 kb on - strand at 102.304 kb on - strand, within HGI48_RS00405 at 102.380 kb on + strand, within HGI48_RS00405 at 102.380 kb on + strand, within HGI48_RS00405 at 102.469 kb on + strand, within HGI48_RS00405 at 102.470 kb on - strand, within HGI48_RS00405 at 102.662 kb on - strand at 102.767 kb on + strand, within HGI48_RS00405 at 102.767 kb on + strand, within HGI48_RS00405 at 102.768 kb on - strand, within HGI48_RS00405 at 102.768 kb on - strand at 102.768 kb on - strand, within HGI48_RS00405 at 102.768 kb on - strand, within HGI48_RS00405 at 102.768 kb on - strand, within HGI48_RS00405 at 102.768 kb on - strand, within HGI48_RS00405 at 102.799 kb on - strand, within HGI48_RS00405 at 102.837 kb on + strand, within HGI48_RS00405 at 102.837 kb on + strand, within HGI48_RS00405
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 100,699 + +1.0 100,700 - +0.3 100,700 - -0.6 100,703 + -0.6 100,703 + +0.7 100,703 + -0.9 100,703 + -2.6 100,703 + -0.6 100,703 + -0.2 100,704 - +0.0 100,704 - -1.4 100,704 - -2.0 100,704 - -1.3 100,704 - +2.0 100,704 - +0.7 100,704 - -1.4 100,724 + +0.8 100,724 + -1.0 100,724 + -1.4 100,724 + -0.2 100,724 + +1.0 100,724 + +0.5 100,724 + -1.6 100,724 + +0.0 100,724 + +0.1 100,724 + +0.8 100,725 - -0.4 100,725 - +0.0 100,730 - -1.0 100,730 - -0.1 100,732 + +0.4 100,733 - +0.4 100,733 - +0.2 100,866 - HGI48_RS00390 0.55 -0.3 100,866 - HGI48_RS00390 0.55 -1.6 101,077 + HGI48_RS00395 0.17 -1.0 101,077 + -0.6 101,077 + -0.6 101,077 + HGI48_RS00395 0.17 -1.8 101,077 + HGI48_RS00395 0.17 -1.6 101,077 + HGI48_RS00395 0.17 -1.0 101,078 - HGI48_RS00395 0.17 +0.8 101,078 - HGI48_RS00395 0.17 +1.8 101,395 + HGI48_RS00395 0.69 +0.0 101,461 - HGI48_RS00395 0.79 -0.0 101,461 - HGI48_RS00395 0.79 -0.2 101,511 + HGI48_RS00395 0.88 +0.1 101,511 + HGI48_RS00395 0.88 -1.7 101,527 + -0.9 101,527 + -0.6 101,528 - +1.6 101,528 - -0.5 101,546 - +0.3 101,546 - -0.4 101,546 - -1.8 101,589 - -1.0 101,812 + HGI48_RS00400 0.61 +0.6 101,837 - HGI48_RS00400 0.75 -1.8 101,879 + +0.0 101,879 + +0.7 101,879 + -1.3 101,879 + -0.6 101,879 + +0.2 101,879 + +1.6 101,879 + -1.0 101,879 + +1.3 101,879 + -0.1 101,880 - +0.0 101,880 - +1.7 101,880 - -0.0 101,881 + +0.5 101,881 + -1.6 101,882 - +0.7 101,882 - -1.1 101,882 - -0.7 101,924 + +0.0 101,924 + -0.8 101,930 + +0.0 101,930 + +0.2 101,930 + -0.5 101,930 + -0.6 101,930 + -0.7 101,930 + -1.4 101,930 + -0.6 101,931 - -1.0 101,931 - -1.5 101,931 - +0.4 101,931 - -0.1 101,931 - -0.3 101,931 - +0.2 101,931 - +0.1 101,931 - -0.3 101,934 - -0.7 101,978 - +0.6 101,978 - -0.8 101,978 - +1.0 102,005 + +0.4 102,005 + HGI48_RS00405 0.12 -0.5 102,005 + HGI48_RS00405 0.12 -0.5 102,005 + HGI48_RS00405 0.12 -0.2 102,006 - HGI48_RS00405 0.12 +0.0 102,090 + HGI48_RS00405 0.20 -0.6 102,090 + HGI48_RS00405 0.20 -1.0 102,090 + HGI48_RS00405 0.20 -0.5 102,090 + HGI48_RS00405 0.20 -1.3 102,090 + HGI48_RS00405 0.20 +1.3 102,090 + HGI48_RS00405 0.20 -0.1 102,091 - +2.6 102,091 - -0.6 102,091 - HGI48_RS00405 0.20 +0.7 102,091 - HGI48_RS00405 0.20 -0.2 102,091 - HGI48_RS00405 0.20 -0.1 102,091 - HGI48_RS00405 0.20 +1.5 102,091 - HGI48_RS00405 0.20 +0.6 102,092 + HGI48_RS00405 0.20 -1.9 102,092 + +1.6 102,092 + -0.6 102,092 + HGI48_RS00405 0.20 -0.6 102,092 + HGI48_RS00405 0.20 -0.3 102,092 + HGI48_RS00405 0.20 -0.8 102,093 - HGI48_RS00405 0.20 +0.1 102,093 - HGI48_RS00405 0.20 +0.0 102,113 + +0.4 102,113 + HGI48_RS00405 0.22 +0.0 102,113 + HGI48_RS00405 0.22 -0.3 102,113 + HGI48_RS00405 0.22 +0.8 102,113 + HGI48_RS00405 0.22 +0.8 102,114 - HGI48_RS00405 0.22 -0.2 102,115 + HGI48_RS00405 0.22 -0.5 102,115 + HGI48_RS00405 0.22 +1.3 102,115 + HGI48_RS00405 0.22 +0.4 102,115 + -0.6 102,115 + -0.6 102,115 + HGI48_RS00405 0.22 -0.9 102,115 + HGI48_RS00405 0.22 +0.8 102,116 - HGI48_RS00405 0.23 +0.3 102,116 - HGI48_RS00405 0.23 -0.3 102,116 - HGI48_RS00405 0.23 -0.2 102,116 - HGI48_RS00405 0.23 +0.4 102,116 - HGI48_RS00405 0.23 +0.3 102,116 - HGI48_RS00405 0.23 +0.0 102,122 + +1.0 102,122 + +2.0 102,122 + HGI48_RS00405 0.23 +1.0 102,122 + HGI48_RS00405 0.23 -0.4 102,123 - HGI48_RS00405 0.23 -0.8 102,123 - HGI48_RS00405 0.23 +0.0 102,123 - HGI48_RS00405 0.23 -1.3 102,124 + HGI48_RS00405 0.23 +0.3 102,124 + HGI48_RS00405 0.23 +0.2 102,125 - HGI48_RS00405 0.23 -1.0 102,125 - HGI48_RS00405 0.23 -0.7 102,125 - HGI48_RS00405 0.23 +0.4 102,125 - +2.0 102,125 - HGI48_RS00405 0.23 +0.0 102,125 - +1.0 102,194 + +2.6 102,194 + HGI48_RS00405 0.30 +0.8 102,194 + +0.0 102,194 + HGI48_RS00405 0.30 +0.6 102,194 + HGI48_RS00405 0.30 +0.8 102,194 + HGI48_RS00405 0.30 -1.0 102,195 - +0.4 102,195 - HGI48_RS00405 0.30 +0.4 102,195 - HGI48_RS00405 0.30 -0.3 102,195 - HGI48_RS00405 0.30 +0.0 102,195 - HGI48_RS00405 0.30 -1.6 102,195 - HGI48_RS00405 0.30 -2.3 102,195 - HGI48_RS00405 0.30 -0.1 102,277 + HGI48_RS00405 0.37 -1.7 102,278 - HGI48_RS00405 0.37 -0.4 102,278 - +0.4 102,304 - HGI48_RS00405 0.40 -1.8 102,380 + HGI48_RS00405 0.47 -0.3 102,380 + HGI48_RS00405 0.47 -0.2 102,469 + HGI48_RS00405 0.55 +0.4 102,470 - HGI48_RS00405 0.55 +0.4 102,662 - +1.4 102,767 + HGI48_RS00405 0.82 +0.8 102,767 + HGI48_RS00405 0.82 -0.4 102,768 - HGI48_RS00405 0.82 -1.0 102,768 - +2.6 102,768 - HGI48_RS00405 0.82 -0.3 102,768 - HGI48_RS00405 0.82 -0.8 102,768 - HGI48_RS00405 0.82 +1.4 102,768 - HGI48_RS00405 0.82 +0.0 102,799 - HGI48_RS00405 0.85 +1.3 102,837 + HGI48_RS00405 0.89 -0.3 102,837 + HGI48_RS00405 0.89 -2.0
Or see this region's nucleotide sequence