Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HGI48_RS18825 and HGI48_RS18830 are separated by 70 nucleotides HGI48_RS18830 and HGI48_RS18835 are separated by 195 nucleotides HGI48_RS18835 and HGI48_RS18840 are separated by 14 nucleotides HGI48_RS18840 and HGI48_RS18845 overlap by 17 nucleotides HGI48_RS18845 and HGI48_RS18850 are separated by 4 nucleotides
HGI48_RS18825: HGI48_RS18825 - S8 family serine peptidase, at 4,223,031 to 4,224,203
_RS18825
HGI48_RS18830: HGI48_RS18830 - IS3 family transposase, at 4,224,274 to 4,224,438
_RS18830
HGI48_RS18835: HGI48_RS18835 - nitrogen fixation protein NifM, at 4,224,634 to 4,225,419
_RS18835
HGI48_RS18840: HGI48_RS18840 - nitrogen fixation protein NifZ, at 4,225,434 to 4,225,877
_RS18840
HGI48_RS18845: HGI48_RS18845 - nitrogen fixation protein NifW, at 4,225,861 to 4,226,130
_RS18845
HGI48_RS18850: HGI48_RS18850 - homocitrate synthase, at 4,226,135 to 4,227,283
_RS18850
Position (kb)
4224
4225
4226 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4223.664 kb on - strand, within HGI48_RS18825 at 4223.840 kb on - strand, within HGI48_RS18825 at 4223.977 kb on + strand, within HGI48_RS18825 at 4224.026 kb on - strand, within HGI48_RS18825 at 4224.061 kb on + strand, within HGI48_RS18825 at 4224.084 kb on - strand, within HGI48_RS18825 at 4224.084 kb on - strand, within HGI48_RS18825 at 4224.097 kb on + strand at 4224.098 kb on - strand at 4224.190 kb on + strand at 4224.202 kb on - strand at 4224.214 kb on - strand at 4224.258 kb on + strand at 4224.362 kb on + strand, within HGI48_RS18830 at 4224.362 kb on + strand, within HGI48_RS18830 at 4224.363 kb on - strand, within HGI48_RS18830 at 4224.366 kb on + strand, within HGI48_RS18830 at 4224.366 kb on + strand, within HGI48_RS18830 at 4224.367 kb on - strand, within HGI48_RS18830 at 4224.385 kb on + strand, within HGI48_RS18830 at 4224.417 kb on + strand, within HGI48_RS18830 at 4224.418 kb on - strand at 4224.424 kb on + strand at 4224.452 kb on + strand at 4224.519 kb on + strand at 4224.519 kb on + strand at 4224.519 kb on + strand at 4224.519 kb on + strand at 4224.520 kb on - strand at 4224.520 kb on - strand at 4224.520 kb on - strand at 4224.520 kb on - strand at 4224.568 kb on + strand at 4224.590 kb on + strand at 4224.591 kb on - strand at 4224.591 kb on - strand at 4224.636 kb on - strand at 4224.636 kb on - strand at 4224.690 kb on - strand at 4224.690 kb on - strand at 4224.784 kb on + strand, within HGI48_RS18835 at 4225.342 kb on + strand at 4225.342 kb on + strand at 4225.343 kb on - strand at 4225.343 kb on - strand at 4225.456 kb on + strand at 4225.456 kb on + strand at 4225.457 kb on - strand at 4225.457 kb on - strand at 4225.457 kb on - strand at 4225.505 kb on + strand, within HGI48_RS18840 at 4225.605 kb on - strand, within HGI48_RS18840 at 4225.605 kb on - strand, within HGI48_RS18840 at 4225.622 kb on - strand, within HGI48_RS18840 at 4225.717 kb on + strand, within HGI48_RS18840 at 4225.717 kb on + strand, within HGI48_RS18840 at 4225.718 kb on - strand, within HGI48_RS18840 at 4225.718 kb on - strand, within HGI48_RS18840 at 4225.721 kb on + strand, within HGI48_RS18840 at 4225.721 kb on + strand, within HGI48_RS18840 at 4225.721 kb on + strand, within HGI48_RS18840 at 4225.722 kb on - strand at 4225.759 kb on + strand, within HGI48_RS18840 at 4225.759 kb on + strand, within HGI48_RS18840 at 4225.760 kb on - strand at 4225.760 kb on - strand, within HGI48_RS18840 at 4225.760 kb on - strand, within HGI48_RS18840 at 4225.760 kb on - strand, within HGI48_RS18840 at 4225.760 kb on - strand, within HGI48_RS18840 at 4225.904 kb on + strand, within HGI48_RS18845 at 4225.905 kb on - strand, within HGI48_RS18845 at 4226.051 kb on + strand, within HGI48_RS18845 at 4226.052 kb on - strand, within HGI48_RS18845 at 4226.117 kb on + strand at 4226.118 kb on - strand at 4226.184 kb on + strand at 4226.184 kb on + strand at 4226.184 kb on + strand at 4226.184 kb on + strand at 4226.184 kb on + strand at 4226.185 kb on - strand at 4226.185 kb on - strand at 4226.185 kb on - strand at 4226.388 kb on + strand, within HGI48_RS18850 at 4226.389 kb on - strand, within HGI48_RS18850 at 4226.389 kb on - strand, within HGI48_RS18850 at 4226.389 kb on - strand, within HGI48_RS18850 at 4226.389 kb on - strand, within HGI48_RS18850 at 4226.389 kb on - strand, within HGI48_RS18850
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 4,223,664 - HGI48_RS18825 0.54 +0.3 4,223,840 - HGI48_RS18825 0.69 +1.1 4,223,977 + HGI48_RS18825 0.81 -1.1 4,224,026 - HGI48_RS18825 0.85 -2.0 4,224,061 + HGI48_RS18825 0.88 +0.7 4,224,084 - HGI48_RS18825 0.90 -1.2 4,224,084 - HGI48_RS18825 0.90 +0.2 4,224,097 + -1.2 4,224,098 - +0.5 4,224,190 + -0.4 4,224,202 - +0.1 4,224,214 - -1.4 4,224,258 + -0.7 4,224,362 + HGI48_RS18830 0.53 -1.0 4,224,362 + HGI48_RS18830 0.53 -0.4 4,224,363 - HGI48_RS18830 0.54 -1.2 4,224,366 + HGI48_RS18830 0.56 +1.5 4,224,366 + HGI48_RS18830 0.56 +0.8 4,224,367 - HGI48_RS18830 0.56 +0.2 4,224,385 + HGI48_RS18830 0.67 -0.6 4,224,417 + HGI48_RS18830 0.87 -0.4 4,224,418 - +1.3 4,224,424 + -0.8 4,224,452 + -0.1 4,224,519 + -0.4 4,224,519 + -1.2 4,224,519 + -0.7 4,224,519 + +0.8 4,224,520 - +0.8 4,224,520 - +0.5 4,224,520 - +1.0 4,224,520 - +0.3 4,224,568 + -2.2 4,224,590 + -0.0 4,224,591 - +0.1 4,224,591 - +0.6 4,224,636 - -0.2 4,224,636 - -0.8 4,224,690 - -1.4 4,224,690 - +2.0 4,224,784 + HGI48_RS18835 0.19 -0.7 4,225,342 + +0.0 4,225,342 + -0.2 4,225,343 - -1.7 4,225,343 - -0.2 4,225,456 + -1.2 4,225,456 + +1.8 4,225,457 - -0.2 4,225,457 - -0.0 4,225,457 - +2.0 4,225,505 + HGI48_RS18840 0.16 +0.5 4,225,605 - HGI48_RS18840 0.39 +0.7 4,225,605 - HGI48_RS18840 0.39 -0.2 4,225,622 - HGI48_RS18840 0.42 +0.0 4,225,717 + HGI48_RS18840 0.64 -0.2 4,225,717 + HGI48_RS18840 0.64 +1.0 4,225,718 - HGI48_RS18840 0.64 -0.2 4,225,718 - HGI48_RS18840 0.64 +1.0 4,225,721 + HGI48_RS18840 0.65 -1.5 4,225,721 + HGI48_RS18840 0.65 +0.8 4,225,721 + HGI48_RS18840 0.65 -0.1 4,225,722 - +1.5 4,225,759 + HGI48_RS18840 0.73 -0.3 4,225,759 + HGI48_RS18840 0.73 -1.8 4,225,760 - +0.2 4,225,760 - HGI48_RS18840 0.73 +1.6 4,225,760 - HGI48_RS18840 0.73 +0.8 4,225,760 - HGI48_RS18840 0.73 +1.9 4,225,760 - HGI48_RS18840 0.73 -0.6 4,225,904 + HGI48_RS18845 0.16 -0.5 4,225,905 - HGI48_RS18845 0.16 -0.2 4,226,051 + HGI48_RS18845 0.70 +0.1 4,226,052 - HGI48_RS18845 0.71 +0.8 4,226,117 + -0.8 4,226,118 - +1.1 4,226,184 + +0.1 4,226,184 + +0.3 4,226,184 + +1.0 4,226,184 + +0.2 4,226,184 + +0.8 4,226,185 - -0.2 4,226,185 - -1.4 4,226,185 - -0.2 4,226,388 + HGI48_RS18850 0.22 +0.3 4,226,389 - HGI48_RS18850 0.22 +0.1 4,226,389 - HGI48_RS18850 0.22 -0.6 4,226,389 - HGI48_RS18850 0.22 -1.8 4,226,389 - HGI48_RS18850 0.22 -1.2 4,226,389 - HGI48_RS18850 0.22 +0.3
Or see this region's nucleotide sequence