Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HGI48_RS10300 and HGI48_RS10305 are separated by 266 nucleotides HGI48_RS10305 and HGI48_RS10310 are separated by 84 nucleotides HGI48_RS10310 and HGI48_RS10315 are separated by 5 nucleotides HGI48_RS10315 and HGI48_RS10320 are separated by 45 nucleotides
HGI48_RS10300: HGI48_RS10300 - signal peptide peptidase SppA, at 2,221,733 to 2,223,577
_RS10300
HGI48_RS10305: HGI48_RS10305 - NAD(P)H nitroreductase, at 2,223,844 to 2,224,395
_RS10305
HGI48_RS10310: HGI48_RS10310 - DNA topoisomerase III, at 2,224,480 to 2,226,405
_RS10310
HGI48_RS10315: HGI48_RS10315 - DUF1496 domain-containing protein, at 2,226,411 to 2,226,806
_RS10315
HGI48_RS10320: HGI48_RS10320 - exodeoxyribonuclease III, at 2,226,852 to 2,227,658
_RS10320
Position (kb)
2224
2225
2226
2227 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2223.546 kb on - strand at 2223.606 kb on + strand at 2223.695 kb on - strand at 2223.755 kb on - strand at 2223.774 kb on - strand at 2223.991 kb on - strand, within HGI48_RS10305 at 2224.143 kb on + strand, within HGI48_RS10305 at 2224.318 kb on + strand, within HGI48_RS10305 at 2224.318 kb on + strand, within HGI48_RS10305 at 2224.319 kb on - strand, within HGI48_RS10305 at 2224.338 kb on + strand, within HGI48_RS10305 at 2224.368 kb on + strand at 2224.388 kb on - strand at 2224.388 kb on - strand at 2224.388 kb on - strand at 2224.388 kb on - strand at 2224.394 kb on - strand at 2224.403 kb on - strand at 2224.567 kb on - strand at 2224.639 kb on + strand at 2224.640 kb on - strand at 2224.640 kb on - strand at 2224.809 kb on - strand, within HGI48_RS10310 at 2224.831 kb on + strand, within HGI48_RS10310 at 2225.017 kb on + strand, within HGI48_RS10310 at 2225.017 kb on + strand, within HGI48_RS10310 at 2225.018 kb on - strand at 2225.018 kb on - strand, within HGI48_RS10310 at 2225.018 kb on - strand, within HGI48_RS10310 at 2225.019 kb on + strand, within HGI48_RS10310 at 2225.019 kb on + strand, within HGI48_RS10310 at 2225.019 kb on + strand, within HGI48_RS10310 at 2225.020 kb on - strand, within HGI48_RS10310 at 2225.314 kb on + strand, within HGI48_RS10310 at 2225.315 kb on - strand, within HGI48_RS10310 at 2225.316 kb on + strand, within HGI48_RS10310 at 2225.317 kb on - strand, within HGI48_RS10310 at 2225.317 kb on - strand at 2225.317 kb on - strand, within HGI48_RS10310 at 2225.323 kb on - strand, within HGI48_RS10310 at 2225.437 kb on + strand, within HGI48_RS10310 at 2225.457 kb on + strand, within HGI48_RS10310 at 2225.458 kb on - strand, within HGI48_RS10310 at 2225.458 kb on - strand, within HGI48_RS10310 at 2225.686 kb on + strand, within HGI48_RS10310 at 2225.686 kb on + strand at 2225.707 kb on + strand, within HGI48_RS10310 at 2225.707 kb on + strand, within HGI48_RS10310 at 2225.708 kb on - strand, within HGI48_RS10310 at 2225.742 kb on + strand, within HGI48_RS10310 at 2225.743 kb on - strand, within HGI48_RS10310 at 2226.118 kb on + strand at 2226.120 kb on + strand, within HGI48_RS10310 at 2226.120 kb on + strand, within HGI48_RS10310 at 2226.120 kb on + strand, within HGI48_RS10310 at 2226.120 kb on + strand, within HGI48_RS10310 at 2226.121 kb on - strand at 2226.285 kb on + strand at 2226.285 kb on + strand at 2226.285 kb on + strand at 2226.286 kb on - strand at 2226.286 kb on - strand at 2226.286 kb on - strand at 2226.312 kb on + strand at 2226.312 kb on + strand at 2226.312 kb on + strand at 2226.312 kb on + strand at 2226.313 kb on - strand at 2226.403 kb on + strand at 2226.454 kb on + strand, within HGI48_RS10315 at 2226.455 kb on - strand, within HGI48_RS10315 at 2226.494 kb on + strand, within HGI48_RS10315 at 2226.694 kb on - strand, within HGI48_RS10315 at 2226.694 kb on - strand at 2226.694 kb on - strand at 2226.804 kb on + strand at 2226.810 kb on + strand at 2226.965 kb on + strand, within HGI48_RS10320 at 2226.965 kb on + strand at 2227.215 kb on - strand, within HGI48_RS10320 at 2227.273 kb on + strand at 2227.274 kb on - strand, within HGI48_RS10320
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 2,223,546 - -2.0 2,223,606 + -0.7 2,223,695 - +0.0 2,223,755 - -2.0 2,223,774 - +1.4 2,223,991 - HGI48_RS10305 0.27 -0.0 2,224,143 + HGI48_RS10305 0.54 -1.3 2,224,318 + HGI48_RS10305 0.86 -0.3 2,224,318 + HGI48_RS10305 0.86 -0.4 2,224,319 - HGI48_RS10305 0.86 -1.7 2,224,338 + HGI48_RS10305 0.89 +0.3 2,224,368 + -0.2 2,224,388 - -2.9 2,224,388 - +0.3 2,224,388 - -0.4 2,224,388 - -0.9 2,224,394 - +0.9 2,224,403 - -0.1 2,224,567 - -0.1 2,224,639 + -1.7 2,224,640 - +0.6 2,224,640 - -1.2 2,224,809 - HGI48_RS10310 0.17 -0.1 2,224,831 + HGI48_RS10310 0.18 -0.1 2,225,017 + HGI48_RS10310 0.28 +0.3 2,225,017 + HGI48_RS10310 0.28 -1.7 2,225,018 - +0.9 2,225,018 - HGI48_RS10310 0.28 +0.6 2,225,018 - HGI48_RS10310 0.28 +0.1 2,225,019 + HGI48_RS10310 0.28 +0.6 2,225,019 + HGI48_RS10310 0.28 -0.9 2,225,019 + HGI48_RS10310 0.28 -0.0 2,225,020 - HGI48_RS10310 0.28 +0.2 2,225,314 + HGI48_RS10310 0.43 -0.9 2,225,315 - HGI48_RS10310 0.43 -0.4 2,225,316 + HGI48_RS10310 0.43 -0.3 2,225,317 - HGI48_RS10310 0.43 -1.0 2,225,317 - +2.3 2,225,317 - HGI48_RS10310 0.43 -0.3 2,225,323 - HGI48_RS10310 0.44 -2.0 2,225,437 + HGI48_RS10310 0.50 -1.3 2,225,457 + HGI48_RS10310 0.51 +0.5 2,225,458 - HGI48_RS10310 0.51 +0.8 2,225,458 - HGI48_RS10310 0.51 -0.5 2,225,686 + HGI48_RS10310 0.63 -2.7 2,225,686 + -0.3 2,225,707 + HGI48_RS10310 0.64 -0.0 2,225,707 + HGI48_RS10310 0.64 -1.7 2,225,708 - HGI48_RS10310 0.64 +0.6 2,225,742 + HGI48_RS10310 0.66 -0.2 2,225,743 - HGI48_RS10310 0.66 -0.5 2,226,118 + +1.6 2,226,120 + HGI48_RS10310 0.85 -0.1 2,226,120 + HGI48_RS10310 0.85 +0.3 2,226,120 + HGI48_RS10310 0.85 -0.5 2,226,120 + HGI48_RS10310 0.85 +1.3 2,226,121 - -1.3 2,226,285 + -0.4 2,226,285 + +1.3 2,226,285 + -1.4 2,226,286 - +0.1 2,226,286 - -0.4 2,226,286 - -0.1 2,226,312 + +0.3 2,226,312 + -1.7 2,226,312 + -0.2 2,226,312 + +0.0 2,226,313 - +0.6 2,226,403 + +0.3 2,226,454 + HGI48_RS10315 0.11 +0.0 2,226,455 - HGI48_RS10315 0.11 +0.3 2,226,494 + HGI48_RS10315 0.21 -0.6 2,226,694 - HGI48_RS10315 0.71 +0.9 2,226,694 - -1.3 2,226,694 - +0.7 2,226,804 + -1.3 2,226,810 + -0.6 2,226,965 + HGI48_RS10320 0.14 -0.0 2,226,965 + -0.3 2,227,215 - HGI48_RS10320 0.45 +0.1 2,227,273 + +0.3 2,227,274 - HGI48_RS10320 0.52 +0.6
Or see this region's nucleotide sequence