Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HGI48_RS05170 and HGI48_RS05175 are separated by 3 nucleotides HGI48_RS05175 and HGI48_RS05180 are separated by 3 nucleotides HGI48_RS05180 and HGI48_RS05185 overlap by 4 nucleotides HGI48_RS05185 and HGI48_RS05190 are separated by 75 nucleotides
HGI48_RS05170: HGI48_RS05170 - 3-hydroxyacyl-ACP dehydratase FabZ, at 1,084,542 to 1,084,997
_RS05170
HGI48_RS05175: HGI48_RS05175 - acyl-ACP--UDP-N-acetylglucosamine O-acyltransferase, at 1,085,001 to 1,085,789
_RS05175
HGI48_RS05180: HGI48_RS05180 - lipid-A-disaccharide synthase, at 1,085,793 to 1,086,941
_RS05180
HGI48_RS05185: HGI48_RS05185 - ribonuclease HII, at 1,086,938 to 1,087,534
_RS05185
HGI48_RS05190: HGI48_RS05190 - DNA polymerase III subunit alpha, at 1,087,610 to 1,091,092
_RS05190
Position (kb) 1085
1086
1087 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 1085.792 kb on + strand at 1085.792 kb on + strand at 1086.973 kb on + strand at 1086.974 kb on - strand at 1086.974 kb on - strand at 1086.974 kb on - strand at 1086.974 kb on - strand at 1086.993 kb on + strand at 1086.993 kb on + strand at 1086.994 kb on - strand at 1087.184 kb on - strand at 1087.445 kb on + strand, within HGI48_RS05185 at 1087.445 kb on + strand at 1087.445 kb on + strand at 1087.537 kb on + strand at 1087.554 kb on + strand at 1087.554 kb on + strand at 1087.554 kb on + strand at 1087.554 kb on + strand at 1087.554 kb on + strand at 1087.554 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 1,085,792 + -0.8 1,085,792 + -0.5 1,086,973 + -2.0 1,086,974 - +1.5 1,086,974 - +0.5 1,086,974 - +0.3 1,086,974 - +0.6 1,086,993 + -0.6 1,086,993 + +0.6 1,086,994 - -3.0 1,087,184 - +1.2 1,087,445 + HGI48_RS05185 0.85 +0.2 1,087,445 + +1.9 1,087,445 + +1.8 1,087,537 + +1.3 1,087,554 + -1.0 1,087,554 + +0.2 1,087,554 + -1.0 1,087,554 + +1.4 1,087,554 + -1.4 1,087,554 + +0.1
Or see this region's nucleotide sequence