Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HGI48_RS01175 and HGI48_RS01180 are separated by 105 nucleotides HGI48_RS01180 and HGI48_RS01185 are separated by 55 nucleotides HGI48_RS01185 and HGI48_RS01190 overlap by 1 nucleotides HGI48_RS01190 and HGI48_RS01195 are separated by 91 nucleotides
HGI48_RS01175: HGI48_RS01175 - sigma D regulator, at 263,985 to 264,434
_RS01175
HGI48_RS01180: HGI48_RS01180 - NAD(+) diphosphatase, at 264,540 to 265,313
_RS01180
HGI48_RS01185: HGI48_RS01185 - uroporphyrinogen decarboxylase, at 265,369 to 266,442
_RS01185
HGI48_RS01190: HGI48_RS01190 - deoxyribonuclease V, at 266,442 to 267,116
_RS01190
HGI48_RS01195: HGI48_RS01195 - YjaG family protein, at 267,208 to 267,798
_RS01195
Position (kb)
265
266
267 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 264.388 kb on + strand, within HGI48_RS01175 at 264.388 kb on + strand, within HGI48_RS01175 at 264.388 kb on + strand, within HGI48_RS01175 at 264.389 kb on - strand, within HGI48_RS01175 at 264.389 kb on - strand, within HGI48_RS01175 at 264.389 kb on - strand, within HGI48_RS01175 at 264.389 kb on - strand, within HGI48_RS01175 at 264.420 kb on + strand at 264.421 kb on - strand at 264.421 kb on - strand at 264.421 kb on - strand at 264.454 kb on + strand at 264.455 kb on - strand at 264.455 kb on - strand at 264.455 kb on - strand at 264.475 kb on + strand at 264.481 kb on + strand at 264.482 kb on - strand at 264.482 kb on - strand at 264.485 kb on + strand at 264.485 kb on + strand at 264.486 kb on - strand at 264.493 kb on + strand at 264.494 kb on - strand at 264.499 kb on + strand at 264.499 kb on + strand at 264.499 kb on + strand at 264.518 kb on + strand at 264.519 kb on - strand at 264.533 kb on - strand at 264.585 kb on + strand at 264.585 kb on + strand at 264.586 kb on - strand at 264.623 kb on - strand at 264.719 kb on + strand, within HGI48_RS01180 at 264.719 kb on + strand at 264.814 kb on - strand, within HGI48_RS01180 at 264.814 kb on - strand, within HGI48_RS01180 at 264.875 kb on - strand, within HGI48_RS01180 at 264.896 kb on + strand, within HGI48_RS01180 at 264.897 kb on - strand, within HGI48_RS01180 at 264.907 kb on - strand, within HGI48_RS01180 at 264.999 kb on + strand, within HGI48_RS01180 at 264.999 kb on + strand, within HGI48_RS01180 at 264.999 kb on + strand, within HGI48_RS01180 at 264.999 kb on + strand, within HGI48_RS01180 at 264.999 kb on + strand, within HGI48_RS01180 at 264.999 kb on + strand, within HGI48_RS01180 at 264.999 kb on + strand at 264.999 kb on + strand, within HGI48_RS01180 at 264.999 kb on + strand at 265.000 kb on - strand, within HGI48_RS01180 at 265.000 kb on - strand, within HGI48_RS01180 at 265.000 kb on - strand at 265.000 kb on - strand, within HGI48_RS01180 at 265.000 kb on - strand, within HGI48_RS01180 at 265.000 kb on - strand, within HGI48_RS01180 at 265.080 kb on - strand, within HGI48_RS01180 at 265.101 kb on + strand, within HGI48_RS01180 at 265.101 kb on + strand, within HGI48_RS01180 at 265.101 kb on + strand, within HGI48_RS01180 at 265.102 kb on - strand, within HGI48_RS01180 at 265.102 kb on - strand, within HGI48_RS01180 at 265.102 kb on - strand, within HGI48_RS01180 at 265.102 kb on - strand, within HGI48_RS01180 at 265.161 kb on + strand, within HGI48_RS01180 at 265.221 kb on + strand, within HGI48_RS01180 at 265.222 kb on - strand, within HGI48_RS01180 at 265.286 kb on - strand at 265.288 kb on + strand at 265.288 kb on + strand at 265.288 kb on + strand at 265.289 kb on - strand at 265.289 kb on - strand at 265.289 kb on - strand at 265.355 kb on - strand at 266.422 kb on + strand at 266.448 kb on - strand at 266.507 kb on + strand at 266.507 kb on + strand at 266.507 kb on + strand at 266.507 kb on + strand at 266.508 kb on - strand at 266.508 kb on - strand at 266.625 kb on + strand, within HGI48_RS01190 at 266.626 kb on - strand at 266.640 kb on - strand, within HGI48_RS01190 at 266.658 kb on + strand, within HGI48_RS01190 at 266.658 kb on + strand, within HGI48_RS01190 at 266.658 kb on + strand, within HGI48_RS01190 at 266.658 kb on + strand, within HGI48_RS01190 at 266.658 kb on + strand, within HGI48_RS01190 at 266.658 kb on + strand, within HGI48_RS01190 at 266.658 kb on + strand, within HGI48_RS01190 at 266.658 kb on + strand, within HGI48_RS01190 at 266.658 kb on + strand, within HGI48_RS01190 at 266.658 kb on + strand, within HGI48_RS01190 at 266.658 kb on + strand, within HGI48_RS01190 at 266.658 kb on + strand, within HGI48_RS01190 at 266.658 kb on + strand, within HGI48_RS01190 at 266.658 kb on + strand, within HGI48_RS01190 at 266.658 kb on + strand, within HGI48_RS01190 at 266.658 kb on + strand at 266.658 kb on + strand, within HGI48_RS01190 at 266.658 kb on + strand, within HGI48_RS01190 at 266.658 kb on + strand, within HGI48_RS01190 at 266.658 kb on + strand, within HGI48_RS01190 at 266.658 kb on + strand, within HGI48_RS01190 at 266.659 kb on - strand, within HGI48_RS01190 at 266.659 kb on - strand, within HGI48_RS01190 at 266.659 kb on - strand, within HGI48_RS01190 at 266.659 kb on - strand, within HGI48_RS01190 at 266.659 kb on - strand, within HGI48_RS01190 at 266.659 kb on - strand at 266.659 kb on - strand, within HGI48_RS01190 at 266.659 kb on - strand, within HGI48_RS01190 at 266.659 kb on - strand at 266.659 kb on - strand, within HGI48_RS01190 at 266.659 kb on - strand, within HGI48_RS01190 at 266.659 kb on - strand, within HGI48_RS01190 at 266.659 kb on - strand, within HGI48_RS01190 at 266.659 kb on - strand, within HGI48_RS01190 at 266.659 kb on - strand, within HGI48_RS01190 at 266.660 kb on + strand, within HGI48_RS01190 at 266.660 kb on + strand, within HGI48_RS01190 at 266.660 kb on + strand, within HGI48_RS01190 at 266.660 kb on + strand, within HGI48_RS01190 at 266.661 kb on - strand, within HGI48_RS01190 at 266.661 kb on - strand, within HGI48_RS01190 at 266.661 kb on - strand, within HGI48_RS01190 at 266.661 kb on - strand, within HGI48_RS01190 at 267.114 kb on + strand at 267.114 kb on + strand at 267.115 kb on - strand at 267.115 kb on - strand at 267.115 kb on - strand at 267.115 kb on - strand at 267.140 kb on - strand at 267.151 kb on + strand at 267.151 kb on + strand at 267.151 kb on + strand at 267.153 kb on + strand at 267.176 kb on + strand at 267.188 kb on + strand at 267.188 kb on + strand at 267.188 kb on + strand at 267.191 kb on + strand at 267.191 kb on + strand at 267.191 kb on + strand at 267.192 kb on - strand at 267.192 kb on - strand at 267.202 kb on + strand at 267.203 kb on - strand at 267.213 kb on - strand at 267.291 kb on + strand, within HGI48_RS01195 at 267.291 kb on + strand, within HGI48_RS01195 at 267.292 kb on - strand, within HGI48_RS01195 at 267.292 kb on - strand, within HGI48_RS01195 at 267.292 kb on - strand, within HGI48_RS01195 at 267.292 kb on - strand, within HGI48_RS01195 at 267.292 kb on - strand, within HGI48_RS01195 at 267.292 kb on - strand, within HGI48_RS01195 at 267.292 kb on - strand, within HGI48_RS01195 at 267.292 kb on - strand, within HGI48_RS01195 at 267.292 kb on - strand, within HGI48_RS01195 at 267.292 kb on - strand, within HGI48_RS01195 at 267.295 kb on + strand, within HGI48_RS01195 at 267.304 kb on + strand, within HGI48_RS01195 at 267.304 kb on + strand, within HGI48_RS01195 at 267.305 kb on - strand, within HGI48_RS01195 at 267.305 kb on - strand, within HGI48_RS01195 at 267.308 kb on - strand at 267.358 kb on - strand, within HGI48_RS01195
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 264,388 + HGI48_RS01175 0.90 +0.4 264,388 + HGI48_RS01175 0.90 +0.1 264,388 + HGI48_RS01175 0.90 +0.1 264,389 - HGI48_RS01175 0.90 -0.4 264,389 - HGI48_RS01175 0.90 +2.4 264,389 - HGI48_RS01175 0.90 +0.1 264,389 - HGI48_RS01175 0.90 +1.2 264,420 + -0.6 264,421 - +0.4 264,421 - -1.0 264,421 - -0.2 264,454 + +0.4 264,455 - +2.4 264,455 - -1.2 264,455 - -1.2 264,475 + -0.2 264,481 + +0.5 264,482 - -2.3 264,482 - +0.2 264,485 + +0.8 264,485 + -0.6 264,486 - -0.8 264,493 + -0.0 264,494 - +0.2 264,499 + -0.4 264,499 + -0.8 264,499 + +0.4 264,518 + +1.0 264,519 - -2.5 264,533 - -1.4 264,585 + +0.6 264,585 + -0.6 264,586 - +0.8 264,623 - +2.4 264,719 + HGI48_RS01180 0.23 -1.8 264,719 + +0.2 264,814 - HGI48_RS01180 0.35 -0.4 264,814 - HGI48_RS01180 0.35 +0.7 264,875 - HGI48_RS01180 0.43 -1.2 264,896 + HGI48_RS01180 0.46 -0.2 264,897 - HGI48_RS01180 0.46 -0.2 264,907 - HGI48_RS01180 0.47 -0.8 264,999 + HGI48_RS01180 0.59 -1.0 264,999 + HGI48_RS01180 0.59 -0.6 264,999 + HGI48_RS01180 0.59 +1.1 264,999 + HGI48_RS01180 0.59 +0.4 264,999 + HGI48_RS01180 0.59 -0.3 264,999 + HGI48_RS01180 0.59 +0.6 264,999 + -0.8 264,999 + HGI48_RS01180 0.59 +0.4 264,999 + -0.2 265,000 - HGI48_RS01180 0.59 +1.3 265,000 - HGI48_RS01180 0.59 -0.5 265,000 - +2.6 265,000 - HGI48_RS01180 0.59 -1.0 265,000 - HGI48_RS01180 0.59 +0.5 265,000 - HGI48_RS01180 0.59 -0.5 265,080 - HGI48_RS01180 0.70 +1.6 265,101 + HGI48_RS01180 0.72 -0.2 265,101 + HGI48_RS01180 0.72 -0.2 265,101 + HGI48_RS01180 0.72 -2.0 265,102 - HGI48_RS01180 0.73 -0.4 265,102 - HGI48_RS01180 0.73 -0.1 265,102 - HGI48_RS01180 0.73 -0.4 265,102 - HGI48_RS01180 0.73 -0.6 265,161 + HGI48_RS01180 0.80 -1.0 265,221 + HGI48_RS01180 0.88 -0.0 265,222 - HGI48_RS01180 0.88 -0.4 265,286 - -0.5 265,288 + -0.4 265,288 + -0.5 265,288 + +0.3 265,289 - -1.2 265,289 - -0.8 265,289 - +0.2 265,355 - -0.2 266,422 + -0.0 266,448 - -2.2 266,507 + +0.6 266,507 + -1.8 266,507 + -0.6 266,507 + -1.0 266,508 - +1.4 266,508 - -0.4 266,625 + HGI48_RS01190 0.27 +0.1 266,626 - +1.2 266,640 - HGI48_RS01190 0.29 +0.8 266,658 + HGI48_RS01190 0.32 +0.5 266,658 + HGI48_RS01190 0.32 +1.4 266,658 + HGI48_RS01190 0.32 -1.3 266,658 + HGI48_RS01190 0.32 +0.6 266,658 + HGI48_RS01190 0.32 -0.4 266,658 + HGI48_RS01190 0.32 -1.5 266,658 + HGI48_RS01190 0.32 +1.3 266,658 + HGI48_RS01190 0.32 -0.3 266,658 + HGI48_RS01190 0.32 -1.8 266,658 + HGI48_RS01190 0.32 +0.1 266,658 + HGI48_RS01190 0.32 +0.4 266,658 + HGI48_RS01190 0.32 +0.8 266,658 + HGI48_RS01190 0.32 -1.3 266,658 + HGI48_RS01190 0.32 +0.8 266,658 + HGI48_RS01190 0.32 -0.2 266,658 + +0.8 266,658 + HGI48_RS01190 0.32 -0.3 266,658 + HGI48_RS01190 0.32 +1.1 266,658 + HGI48_RS01190 0.32 -0.8 266,658 + HGI48_RS01190 0.32 -0.6 266,658 + HGI48_RS01190 0.32 -0.6 266,659 - HGI48_RS01190 0.32 +0.5 266,659 - HGI48_RS01190 0.32 -0.4 266,659 - HGI48_RS01190 0.32 -1.0 266,659 - HGI48_RS01190 0.32 -0.4 266,659 - HGI48_RS01190 0.32 +0.4 266,659 - +2.8 266,659 - HGI48_RS01190 0.32 +1.5 266,659 - HGI48_RS01190 0.32 -1.4 266,659 - +1.2 266,659 - HGI48_RS01190 0.32 +0.4 266,659 - HGI48_RS01190 0.32 -1.2 266,659 - HGI48_RS01190 0.32 -0.6 266,659 - HGI48_RS01190 0.32 -0.2 266,659 - HGI48_RS01190 0.32 -2.2 266,659 - HGI48_RS01190 0.32 -1.6 266,660 + HGI48_RS01190 0.32 -1.8 266,660 + HGI48_RS01190 0.32 -0.9 266,660 + HGI48_RS01190 0.32 +0.0 266,660 + HGI48_RS01190 0.32 -0.2 266,661 - HGI48_RS01190 0.32 -0.2 266,661 - HGI48_RS01190 0.32 -1.5 266,661 - HGI48_RS01190 0.32 -0.3 266,661 - HGI48_RS01190 0.32 -0.6 267,114 + -0.6 267,114 + -0.2 267,115 - -2.0 267,115 - +0.3 267,115 - +1.1 267,115 - +1.0 267,140 - +0.4 267,151 + -0.8 267,151 + -0.3 267,151 + +1.4 267,153 + -0.2 267,176 + -0.2 267,188 + +1.3 267,188 + -1.6 267,188 + +0.2 267,191 + -1.2 267,191 + +0.4 267,191 + +2.0 267,192 - +0.4 267,192 - -1.2 267,202 + +0.3 267,203 - -1.5 267,213 - +1.4 267,291 + HGI48_RS01195 0.14 -0.2 267,291 + HGI48_RS01195 0.14 +0.1 267,292 - HGI48_RS01195 0.14 -1.5 267,292 - HGI48_RS01195 0.14 -2.2 267,292 - HGI48_RS01195 0.14 -1.8 267,292 - HGI48_RS01195 0.14 +0.8 267,292 - HGI48_RS01195 0.14 -0.0 267,292 - HGI48_RS01195 0.14 -0.2 267,292 - HGI48_RS01195 0.14 -1.8 267,292 - HGI48_RS01195 0.14 -0.3 267,292 - HGI48_RS01195 0.14 +0.8 267,292 - HGI48_RS01195 0.14 -1.2 267,295 + HGI48_RS01195 0.15 +0.8 267,304 + HGI48_RS01195 0.16 -1.3 267,304 + HGI48_RS01195 0.16 -0.8 267,305 - HGI48_RS01195 0.16 +1.0 267,305 - HGI48_RS01195 0.16 -0.1 267,308 - +0.2 267,358 - HGI48_RS01195 0.25 -1.1
Or see this region's nucleotide sequence