Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HGI48_RS19420 and HGI48_RS19425 are separated by 132 nucleotides HGI48_RS19425 and HGI48_RS19430 are separated by 11 nucleotides HGI48_RS19430 and HGI48_RS19435 are separated by 17 nucleotides HGI48_RS19435 and HGI48_RS19440 overlap by 1 nucleotides HGI48_RS19440 and HGI48_RS19445 are separated by 183 nucleotides
HGI48_RS19420: HGI48_RS19420 - 30S ribosomal protein S12, at 4,341,271 to 4,341,645
_RS19420
HGI48_RS19425: HGI48_RS19425 - sulfurtransferase complex subunit TusB, at 4,341,778 to 4,342,065
_RS19425
HGI48_RS19430: HGI48_RS19430 - sulfurtransferase complex subunit TusC, at 4,342,077 to 4,342,436
_RS19430
HGI48_RS19435: HGI48_RS19435 - sulfurtransferase complex subunit TusD, at 4,342,454 to 4,342,843
_RS19435
HGI48_RS19440: HGI48_RS19440 - transcriptional regulator, at 4,342,843 to 4,343,565
_RS19440
HGI48_RS19445: HGI48_RS19445 - phosphoethanolamine transferase EptA, at 4,343,749 to 4,345,392
_RS19445
Position (kb)
4342
4343 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4341.684 kb on - strand at 4341.762 kb on - strand at 4341.764 kb on - strand at 4341.764 kb on - strand at 4341.779 kb on + strand at 4341.780 kb on - strand at 4341.811 kb on + strand, within HGI48_RS19425 at 4341.811 kb on + strand, within HGI48_RS19425 at 4341.830 kb on + strand, within HGI48_RS19425 at 4341.842 kb on - strand, within HGI48_RS19425 at 4341.842 kb on - strand, within HGI48_RS19425 at 4341.868 kb on + strand, within HGI48_RS19425 at 4341.902 kb on + strand, within HGI48_RS19425 at 4342.057 kb on - strand at 4342.099 kb on + strand at 4342.099 kb on + strand at 4342.099 kb on + strand at 4342.100 kb on - strand at 4342.100 kb on - strand at 4342.100 kb on - strand at 4342.100 kb on - strand at 4342.208 kb on - strand, within HGI48_RS19430 at 4342.225 kb on + strand, within HGI48_RS19430 at 4342.225 kb on + strand, within HGI48_RS19430 at 4342.225 kb on + strand, within HGI48_RS19430 at 4342.225 kb on + strand, within HGI48_RS19430 at 4342.225 kb on + strand, within HGI48_RS19430 at 4342.253 kb on + strand, within HGI48_RS19430 at 4342.255 kb on + strand, within HGI48_RS19430 at 4342.255 kb on + strand, within HGI48_RS19430 at 4342.256 kb on - strand, within HGI48_RS19430 at 4342.256 kb on - strand, within HGI48_RS19430 at 4342.412 kb on + strand at 4342.413 kb on - strand at 4342.416 kb on + strand at 4342.520 kb on - strand at 4342.553 kb on + strand, within HGI48_RS19435 at 4342.554 kb on - strand, within HGI48_RS19435 at 4342.554 kb on - strand at 4342.722 kb on + strand, within HGI48_RS19435 at 4342.838 kb on + strand at 4342.838 kb on + strand at 4342.839 kb on - strand at 4342.839 kb on - strand at 4342.839 kb on - strand at 4342.839 kb on - strand at 4342.890 kb on - strand at 4342.890 kb on - strand at 4342.890 kb on - strand at 4342.915 kb on - strand at 4342.974 kb on - strand, within HGI48_RS19440 at 4343.078 kb on - strand, within HGI48_RS19440 at 4343.078 kb on - strand, within HGI48_RS19440 at 4343.078 kb on - strand, within HGI48_RS19440 at 4343.243 kb on - strand, within HGI48_RS19440 at 4343.267 kb on + strand, within HGI48_RS19440 at 4343.268 kb on - strand, within HGI48_RS19440 at 4343.268 kb on - strand, within HGI48_RS19440 at 4343.350 kb on - strand at 4343.377 kb on - strand, within HGI48_RS19440 at 4343.377 kb on - strand at 4343.472 kb on - strand at 4343.472 kb on - strand, within HGI48_RS19440 at 4343.547 kb on + strand at 4343.560 kb on - strand at 4343.560 kb on - strand at 4343.592 kb on - strand at 4343.592 kb on - strand at 4343.592 kb on - strand at 4343.600 kb on - strand at 4343.620 kb on - strand at 4343.626 kb on + strand at 4343.626 kb on + strand at 4343.627 kb on - strand at 4343.627 kb on - strand at 4343.647 kb on + strand at 4343.647 kb on + strand at 4343.647 kb on + strand at 4343.648 kb on - strand at 4343.648 kb on - strand at 4343.679 kb on - strand at 4343.679 kb on - strand at 4343.679 kb on - strand at 4343.679 kb on - strand at 4343.689 kb on + strand at 4343.690 kb on - strand at 4343.690 kb on - strand at 4343.695 kb on - strand at 4343.703 kb on + strand at 4343.703 kb on + strand at 4343.704 kb on - strand at 4343.704 kb on - strand at 4343.704 kb on - strand at 4343.704 kb on - strand at 4343.721 kb on - strand at 4343.721 kb on - strand at 4343.721 kb on - strand at 4343.721 kb on - strand at 4343.793 kb on + strand at 4343.794 kb on - strand at 4343.794 kb on - strand at 4343.797 kb on + strand at 4343.797 kb on + strand at 4343.808 kb on - strand at 4343.831 kb on + strand at 4343.832 kb on - strand at 4343.832 kb on - strand at 4343.839 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 4,341,684 - -2.4 4,341,762 - -1.1 4,341,764 - -0.1 4,341,764 - -1.4 4,341,779 + +0.5 4,341,780 - -1.0 4,341,811 + HGI48_RS19425 0.11 +1.5 4,341,811 + HGI48_RS19425 0.11 -1.0 4,341,830 + HGI48_RS19425 0.18 -1.4 4,341,842 - HGI48_RS19425 0.22 -0.0 4,341,842 - HGI48_RS19425 0.22 -2.6 4,341,868 + HGI48_RS19425 0.31 +0.4 4,341,902 + HGI48_RS19425 0.43 +0.6 4,342,057 - -2.2 4,342,099 + -1.4 4,342,099 + -0.1 4,342,099 + +0.6 4,342,100 - +0.6 4,342,100 - -2.5 4,342,100 - +0.9 4,342,100 - -0.4 4,342,208 - HGI48_RS19430 0.36 +1.0 4,342,225 + HGI48_RS19430 0.41 +0.0 4,342,225 + HGI48_RS19430 0.41 -1.4 4,342,225 + HGI48_RS19430 0.41 -2.1 4,342,225 + HGI48_RS19430 0.41 -0.7 4,342,225 + HGI48_RS19430 0.41 +0.4 4,342,253 + HGI48_RS19430 0.49 +0.3 4,342,255 + HGI48_RS19430 0.49 -0.1 4,342,255 + HGI48_RS19430 0.49 -1.5 4,342,256 - HGI48_RS19430 0.50 -1.2 4,342,256 - HGI48_RS19430 0.50 -0.6 4,342,412 + +0.6 4,342,413 - -0.4 4,342,416 + -2.2 4,342,520 - -0.4 4,342,553 + HGI48_RS19435 0.25 -1.7 4,342,554 - HGI48_RS19435 0.26 -1.6 4,342,554 - +1.0 4,342,722 + HGI48_RS19435 0.69 -0.5 4,342,838 + +0.2 4,342,838 + +0.0 4,342,839 - +0.6 4,342,839 - -0.8 4,342,839 - -0.5 4,342,839 - -1.4 4,342,890 - -1.8 4,342,890 - +0.6 4,342,890 - -1.4 4,342,915 - +1.0 4,342,974 - HGI48_RS19440 0.18 +0.2 4,343,078 - HGI48_RS19440 0.33 -0.4 4,343,078 - HGI48_RS19440 0.33 -1.0 4,343,078 - HGI48_RS19440 0.33 -0.3 4,343,243 - HGI48_RS19440 0.55 -0.3 4,343,267 + HGI48_RS19440 0.59 -2.4 4,343,268 - HGI48_RS19440 0.59 -0.1 4,343,268 - HGI48_RS19440 0.59 +0.6 4,343,350 - -1.0 4,343,377 - HGI48_RS19440 0.74 +1.5 4,343,377 - +1.0 4,343,472 - +0.6 4,343,472 - HGI48_RS19440 0.87 -0.3 4,343,547 + -2.8 4,343,560 - -0.4 4,343,560 - +1.0 4,343,592 - -1.1 4,343,592 - -2.0 4,343,592 - +1.2 4,343,600 - -0.4 4,343,620 - -0.1 4,343,626 + -0.0 4,343,626 + -0.5 4,343,627 - -1.1 4,343,627 - +0.3 4,343,647 + -2.2 4,343,647 + +0.9 4,343,647 + -0.3 4,343,648 - +0.8 4,343,648 - +1.5 4,343,679 - -1.7 4,343,679 - -2.2 4,343,679 - -1.7 4,343,679 - -2.5 4,343,689 + -0.1 4,343,690 - +2.2 4,343,690 - +0.6 4,343,695 - -0.0 4,343,703 + -0.4 4,343,703 + +0.3 4,343,704 - +0.2 4,343,704 - +0.1 4,343,704 - +0.6 4,343,704 - +0.3 4,343,721 - +0.2 4,343,721 - +0.6 4,343,721 - -1.2 4,343,721 - -0.7 4,343,793 + +1.9 4,343,794 - +1.0 4,343,794 - -0.6 4,343,797 + -1.4 4,343,797 + -0.9 4,343,808 - +0.0 4,343,831 + +1.0 4,343,832 - +0.1 4,343,832 - -0.6 4,343,839 - +0.3
Or see this region's nucleotide sequence