Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HGI48_RS18120 and HGI48_RS18125 are separated by 3 nucleotides HGI48_RS18125 and HGI48_RS18130 are separated by 50 nucleotides HGI48_RS18130 and HGI48_RS18135 are separated by 256 nucleotides
HGI48_RS18120: HGI48_RS18120 - acetolactate synthase large subunit, at 4,065,639 to 4,067,303
_RS18120
HGI48_RS18125: HGI48_RS18125 - acetolactate synthase small subunit, at 4,067,307 to 4,067,600
_RS18125
HGI48_RS18130: HGI48_RS18130 - DedA family protein, at 4,067,651 to 4,068,418
_RS18130
HGI48_RS18135: HGI48_RS18135 - N-acetylmuramidase family protein, at 4,068,675 to 4,069,526
_RS18135
Position (kb)
4067
4068
4069 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4066.775 kb on + strand at 4066.776 kb on - strand, within HGI48_RS18120 at 4067.014 kb on + strand, within HGI48_RS18120 at 4067.044 kb on + strand, within HGI48_RS18120 at 4067.044 kb on + strand, within HGI48_RS18120 at 4067.045 kb on - strand, within HGI48_RS18120 at 4067.067 kb on + strand, within HGI48_RS18120 at 4067.068 kb on - strand, within HGI48_RS18120 at 4067.068 kb on - strand, within HGI48_RS18120 at 4067.252 kb on - strand at 4067.301 kb on + strand at 4067.301 kb on + strand at 4067.301 kb on + strand at 4067.302 kb on - strand at 4067.432 kb on + strand, within HGI48_RS18125 at 4067.432 kb on + strand, within HGI48_RS18125 at 4067.432 kb on + strand, within HGI48_RS18125 at 4067.432 kb on + strand, within HGI48_RS18125 at 4067.433 kb on - strand, within HGI48_RS18125 at 4067.507 kb on - strand, within HGI48_RS18125 at 4067.507 kb on - strand at 4067.515 kb on + strand, within HGI48_RS18125 at 4067.567 kb on + strand at 4067.568 kb on - strand, within HGI48_RS18125 at 4067.568 kb on - strand at 4067.592 kb on + strand at 4067.654 kb on + strand at 4067.654 kb on + strand at 4067.654 kb on + strand at 4067.654 kb on + strand at 4067.654 kb on + strand at 4067.654 kb on + strand at 4067.655 kb on - strand at 4067.655 kb on - strand at 4067.685 kb on + strand at 4067.685 kb on + strand at 4067.685 kb on + strand at 4067.686 kb on - strand at 4067.686 kb on - strand at 4067.720 kb on + strand at 4067.720 kb on + strand at 4067.720 kb on + strand at 4067.720 kb on + strand at 4067.721 kb on - strand at 4067.864 kb on - strand, within HGI48_RS18130 at 4067.874 kb on - strand, within HGI48_RS18130 at 4067.887 kb on + strand at 4067.887 kb on + strand, within HGI48_RS18130 at 4067.955 kb on + strand, within HGI48_RS18130 at 4067.955 kb on + strand, within HGI48_RS18130 at 4068.091 kb on + strand at 4068.124 kb on - strand, within HGI48_RS18130 at 4068.129 kb on + strand, within HGI48_RS18130 at 4068.129 kb on + strand, within HGI48_RS18130 at 4068.130 kb on - strand, within HGI48_RS18130 at 4068.130 kb on - strand, within HGI48_RS18130 at 4068.130 kb on - strand, within HGI48_RS18130 at 4068.130 kb on - strand, within HGI48_RS18130 at 4068.160 kb on + strand, within HGI48_RS18130 at 4068.160 kb on + strand, within HGI48_RS18130 at 4068.160 kb on + strand at 4068.160 kb on + strand, within HGI48_RS18130 at 4068.160 kb on + strand at 4068.161 kb on - strand, within HGI48_RS18130 at 4068.161 kb on - strand, within HGI48_RS18130 at 4068.161 kb on - strand, within HGI48_RS18130 at 4068.161 kb on - strand, within HGI48_RS18130 at 4068.195 kb on + strand, within HGI48_RS18130 at 4068.196 kb on - strand, within HGI48_RS18130 at 4068.196 kb on - strand, within HGI48_RS18130 at 4068.260 kb on - strand, within HGI48_RS18130 at 4068.418 kb on + strand at 4068.419 kb on - strand at 4068.426 kb on + strand at 4068.427 kb on - strand at 4068.454 kb on + strand at 4068.466 kb on - strand at 4068.493 kb on + strand at 4068.494 kb on - strand at 4068.496 kb on + strand at 4068.497 kb on - strand at 4068.500 kb on - strand at 4068.526 kb on + strand at 4068.526 kb on + strand at 4068.526 kb on + strand at 4068.526 kb on + strand at 4068.526 kb on + strand at 4068.526 kb on + strand at 4068.527 kb on - strand at 4068.527 kb on - strand at 4068.528 kb on + strand at 4068.580 kb on + strand at 4068.581 kb on - strand at 4068.581 kb on - strand at 4068.581 kb on - strand at 4068.637 kb on + strand at 4068.637 kb on + strand at 4068.638 kb on - strand at 4068.718 kb on + strand at 4068.719 kb on - strand at 4068.719 kb on - strand at 4068.737 kb on - strand at 4068.794 kb on + strand, within HGI48_RS18135 at 4068.794 kb on + strand, within HGI48_RS18135 at 4068.794 kb on + strand, within HGI48_RS18135 at 4068.794 kb on + strand, within HGI48_RS18135 at 4068.795 kb on - strand, within HGI48_RS18135 at 4068.844 kb on + strand, within HGI48_RS18135 at 4068.844 kb on + strand, within HGI48_RS18135 at 4068.844 kb on + strand, within HGI48_RS18135 at 4068.844 kb on + strand, within HGI48_RS18135 at 4068.844 kb on + strand, within HGI48_RS18135 at 4068.844 kb on + strand, within HGI48_RS18135 at 4068.847 kb on + strand, within HGI48_RS18135 at 4068.857 kb on + strand, within HGI48_RS18135 at 4068.869 kb on + strand, within HGI48_RS18135 at 4068.895 kb on + strand at 4069.009 kb on + strand, within HGI48_RS18135 at 4069.009 kb on + strand, within HGI48_RS18135 at 4069.009 kb on + strand, within HGI48_RS18135 at 4069.009 kb on + strand, within HGI48_RS18135 at 4069.009 kb on + strand, within HGI48_RS18135 at 4069.010 kb on - strand, within HGI48_RS18135 at 4069.010 kb on - strand, within HGI48_RS18135 at 4069.061 kb on - strand, within HGI48_RS18135 at 4069.064 kb on + strand, within HGI48_RS18135 at 4069.065 kb on - strand, within HGI48_RS18135 at 4069.096 kb on + strand at 4069.097 kb on - strand, within HGI48_RS18135 at 4069.097 kb on - strand, within HGI48_RS18135 at 4069.137 kb on - strand, within HGI48_RS18135 at 4069.141 kb on - strand, within HGI48_RS18135 at 4069.178 kb on + strand, within HGI48_RS18135 at 4069.178 kb on + strand, within HGI48_RS18135 at 4069.179 kb on - strand at 4069.185 kb on + strand, within HGI48_RS18135 at 4069.296 kb on + strand, within HGI48_RS18135 at 4069.296 kb on + strand, within HGI48_RS18135 at 4069.297 kb on - strand, within HGI48_RS18135 at 4069.299 kb on - strand, within HGI48_RS18135 at 4069.299 kb on - strand, within HGI48_RS18135 at 4069.349 kb on + strand, within HGI48_RS18135 at 4069.349 kb on + strand, within HGI48_RS18135 at 4069.350 kb on - strand at 4069.376 kb on + strand, within HGI48_RS18135 at 4069.402 kb on + strand, within HGI48_RS18135
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 4,066,775 + +0.5 4,066,776 - HGI48_RS18120 0.68 +0.1 4,067,014 + HGI48_RS18120 0.83 -0.1 4,067,044 + HGI48_RS18120 0.84 +0.2 4,067,044 + HGI48_RS18120 0.84 -1.0 4,067,045 - HGI48_RS18120 0.84 -1.8 4,067,067 + HGI48_RS18120 0.86 -0.7 4,067,068 - HGI48_RS18120 0.86 -0.2 4,067,068 - HGI48_RS18120 0.86 -0.3 4,067,252 - -2.1 4,067,301 + +0.9 4,067,301 + -0.5 4,067,301 + +0.2 4,067,302 - +0.7 4,067,432 + HGI48_RS18125 0.43 -1.6 4,067,432 + HGI48_RS18125 0.43 -2.8 4,067,432 + HGI48_RS18125 0.43 -1.5 4,067,432 + HGI48_RS18125 0.43 -1.3 4,067,433 - HGI48_RS18125 0.43 -1.8 4,067,507 - HGI48_RS18125 0.68 -0.5 4,067,507 - +1.5 4,067,515 + HGI48_RS18125 0.71 +0.4 4,067,567 + -0.1 4,067,568 - HGI48_RS18125 0.89 -0.8 4,067,568 - +1.5 4,067,592 + +0.2 4,067,654 + -2.1 4,067,654 + -1.6 4,067,654 + +1.5 4,067,654 + -1.1 4,067,654 + -0.2 4,067,654 + -0.7 4,067,655 - -0.3 4,067,655 - -2.1 4,067,685 + +1.5 4,067,685 + -1.8 4,067,685 + +0.5 4,067,686 - +0.9 4,067,686 - -0.2 4,067,720 + -0.5 4,067,720 + +0.2 4,067,720 + -0.8 4,067,720 + -0.5 4,067,721 - -1.1 4,067,864 - HGI48_RS18130 0.28 -0.3 4,067,874 - HGI48_RS18130 0.29 -0.6 4,067,887 + +2.1 4,067,887 + HGI48_RS18130 0.31 +1.2 4,067,955 + HGI48_RS18130 0.40 -0.5 4,067,955 + HGI48_RS18130 0.40 -0.9 4,068,091 + +1.1 4,068,124 - HGI48_RS18130 0.62 +0.5 4,068,129 + HGI48_RS18130 0.62 +0.5 4,068,129 + HGI48_RS18130 0.62 +1.0 4,068,130 - HGI48_RS18130 0.62 +0.9 4,068,130 - HGI48_RS18130 0.62 +0.8 4,068,130 - HGI48_RS18130 0.62 -1.5 4,068,130 - HGI48_RS18130 0.62 -0.8 4,068,160 + HGI48_RS18130 0.66 -0.5 4,068,160 + HGI48_RS18130 0.66 -0.5 4,068,160 + +0.5 4,068,160 + HGI48_RS18130 0.66 -0.3 4,068,160 + +0.5 4,068,161 - HGI48_RS18130 0.66 -2.1 4,068,161 - HGI48_RS18130 0.66 +1.9 4,068,161 - HGI48_RS18130 0.66 +1.3 4,068,161 - HGI48_RS18130 0.66 -1.8 4,068,195 + HGI48_RS18130 0.71 +0.3 4,068,196 - HGI48_RS18130 0.71 +0.5 4,068,196 - HGI48_RS18130 0.71 -0.2 4,068,260 - HGI48_RS18130 0.79 +0.5 4,068,418 + -0.5 4,068,419 - -1.5 4,068,426 + -0.6 4,068,427 - +1.1 4,068,454 + -1.3 4,068,466 - +0.5 4,068,493 + -1.1 4,068,494 - +0.3 4,068,496 + -0.6 4,068,497 - -0.2 4,068,500 - -1.3 4,068,526 + -1.8 4,068,526 + -0.3 4,068,526 + +0.8 4,068,526 + +0.2 4,068,526 + +0.8 4,068,526 + -0.8 4,068,527 - -0.8 4,068,527 - +0.9 4,068,528 + +0.9 4,068,580 + -0.3 4,068,581 - -2.1 4,068,581 - -2.6 4,068,581 - -1.8 4,068,637 + +0.3 4,068,637 + -1.1 4,068,638 - -1.1 4,068,718 + -0.2 4,068,719 - +0.5 4,068,719 - -1.5 4,068,737 - -2.5 4,068,794 + HGI48_RS18135 0.14 -0.6 4,068,794 + HGI48_RS18135 0.14 -0.1 4,068,794 + HGI48_RS18135 0.14 -2.1 4,068,794 + HGI48_RS18135 0.14 +0.7 4,068,795 - HGI48_RS18135 0.14 -0.7 4,068,844 + HGI48_RS18135 0.20 -0.1 4,068,844 + HGI48_RS18135 0.20 +0.4 4,068,844 + HGI48_RS18135 0.20 -2.1 4,068,844 + HGI48_RS18135 0.20 +0.0 4,068,844 + HGI48_RS18135 0.20 -0.2 4,068,844 + HGI48_RS18135 0.20 +1.2 4,068,847 + HGI48_RS18135 0.20 -0.3 4,068,857 + HGI48_RS18135 0.21 +0.0 4,068,869 + HGI48_RS18135 0.23 +1.3 4,068,895 + +1.9 4,069,009 + HGI48_RS18135 0.39 +1.2 4,069,009 + HGI48_RS18135 0.39 +0.1 4,069,009 + HGI48_RS18135 0.39 -2.6 4,069,009 + HGI48_RS18135 0.39 +0.1 4,069,009 + HGI48_RS18135 0.39 +0.1 4,069,010 - HGI48_RS18135 0.39 -0.7 4,069,010 - HGI48_RS18135 0.39 -0.4 4,069,061 - HGI48_RS18135 0.45 -0.1 4,069,064 + HGI48_RS18135 0.46 -1.0 4,069,065 - HGI48_RS18135 0.46 +1.0 4,069,096 + +0.9 4,069,097 - HGI48_RS18135 0.50 -1.1 4,069,097 - HGI48_RS18135 0.50 +0.2 4,069,137 - HGI48_RS18135 0.54 +0.4 4,069,141 - HGI48_RS18135 0.55 -0.4 4,069,178 + HGI48_RS18135 0.59 -0.1 4,069,178 + HGI48_RS18135 0.59 -0.2 4,069,179 - -0.5 4,069,185 + HGI48_RS18135 0.60 +0.5 4,069,296 + HGI48_RS18135 0.73 +0.6 4,069,296 + HGI48_RS18135 0.73 -0.6 4,069,297 - HGI48_RS18135 0.73 +0.3 4,069,299 - HGI48_RS18135 0.73 -1.2 4,069,299 - HGI48_RS18135 0.73 -1.1 4,069,349 + HGI48_RS18135 0.79 +0.5 4,069,349 + HGI48_RS18135 0.79 -0.2 4,069,350 - +0.9 4,069,376 + HGI48_RS18135 0.82 +0.0 4,069,402 + HGI48_RS18135 0.85 +0.5
Or see this region's nucleotide sequence