Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HGI48_RS17920 and HGI48_RS17925 are separated by 31 nucleotides HGI48_RS17925 and HGI48_RS17930 are separated by 104 nucleotides HGI48_RS17930 and HGI48_RS17935 are separated by 69 nucleotides HGI48_RS17935 and HGI48_RS17940 are separated by 97 nucleotides HGI48_RS17940 and HGI48_RS17945 are separated by 27 nucleotides
HGI48_RS17920: HGI48_RS17920 - cell division protein ZapD, at 4,021,744 to 4,022,493
_RS17920
HGI48_RS17925: HGI48_RS17925 - DNA gyrase inhibitor YacG, at 4,022,525 to 4,022,722
_RS17925
HGI48_RS17930: HGI48_RS17930 - 8-oxo-dGTP diphosphatase MutT, at 4,022,827 to 4,023,228
_RS17930
HGI48_RS17935: HGI48_RS17935 - preprotein translocase subunit SecA, at 4,023,298 to 4,025,991
_RS17935
HGI48_RS17940: HGI48_RS17940 - secA regulator SecM, at 4,026,089 to 4,026,589
_RS17940
HGI48_RS17945: HGI48_RS17945 - DUF721 domain-containing protein, at 4,026,617 to 4,027,138
_RS17945
Position (kb)
4023
4024
4025
4026 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4022.323 kb on + strand, within HGI48_RS17920 at 4022.354 kb on + strand, within HGI48_RS17920 at 4022.354 kb on + strand at 4022.355 kb on - strand, within HGI48_RS17920 at 4022.355 kb on - strand, within HGI48_RS17920 at 4022.355 kb on - strand, within HGI48_RS17920 at 4022.355 kb on - strand at 4022.355 kb on - strand, within HGI48_RS17920 at 4022.397 kb on - strand, within HGI48_RS17920 at 4022.406 kb on + strand, within HGI48_RS17920 at 4022.406 kb on + strand at 4022.406 kb on + strand, within HGI48_RS17920 at 4022.521 kb on + strand at 4022.521 kb on + strand at 4022.521 kb on + strand at 4022.521 kb on + strand at 4022.521 kb on + strand at 4022.521 kb on + strand at 4022.522 kb on - strand at 4022.522 kb on - strand at 4022.522 kb on - strand at 4022.558 kb on - strand at 4022.683 kb on + strand, within HGI48_RS17925 at 4022.683 kb on + strand, within HGI48_RS17925 at 4022.683 kb on + strand, within HGI48_RS17925 at 4022.684 kb on - strand, within HGI48_RS17925 at 4022.684 kb on - strand, within HGI48_RS17925 at 4022.720 kb on + strand at 4022.720 kb on + strand at 4022.720 kb on + strand at 4022.738 kb on + strand at 4022.757 kb on + strand at 4022.758 kb on - strand at 4022.793 kb on + strand at 4022.794 kb on - strand at 4022.794 kb on - strand at 4022.794 kb on - strand at 4022.794 kb on - strand at 4022.799 kb on + strand at 4022.800 kb on - strand at 4022.800 kb on - strand at 4022.811 kb on + strand at 4022.828 kb on + strand at 4022.982 kb on + strand, within HGI48_RS17930 at 4023.129 kb on + strand, within HGI48_RS17930 at 4023.138 kb on + strand, within HGI48_RS17930 at 4023.138 kb on + strand at 4023.138 kb on + strand, within HGI48_RS17930 at 4023.139 kb on - strand, within HGI48_RS17930 at 4023.196 kb on - strand at 4023.196 kb on - strand at 4023.211 kb on + strand at 4023.277 kb on + strand at 4023.300 kb on - strand at 4023.329 kb on + strand at 4023.386 kb on + strand at 4026.054 kb on - strand at 4026.054 kb on - strand at 4026.243 kb on - strand, within HGI48_RS17940 at 4026.243 kb on - strand, within HGI48_RS17940 at 4026.243 kb on - strand, within HGI48_RS17940 at 4026.243 kb on - strand, within HGI48_RS17940 at 4026.243 kb on - strand, within HGI48_RS17940 at 4026.255 kb on - strand, within HGI48_RS17940 at 4026.299 kb on - strand, within HGI48_RS17940 at 4026.329 kb on - strand, within HGI48_RS17940 at 4026.418 kb on - strand, within HGI48_RS17940 at 4026.418 kb on - strand, within HGI48_RS17940 at 4026.554 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 4,022,323 + HGI48_RS17920 0.77 -1.1 4,022,354 + HGI48_RS17920 0.81 -0.3 4,022,354 + +0.5 4,022,355 - HGI48_RS17920 0.81 +0.5 4,022,355 - HGI48_RS17920 0.81 -0.9 4,022,355 - HGI48_RS17920 0.81 +0.1 4,022,355 - -0.5 4,022,355 - HGI48_RS17920 0.81 -0.1 4,022,397 - HGI48_RS17920 0.87 -0.8 4,022,406 + HGI48_RS17920 0.88 +0.5 4,022,406 + +2.3 4,022,406 + HGI48_RS17920 0.88 +0.7 4,022,521 + +0.9 4,022,521 + -0.5 4,022,521 + -0.9 4,022,521 + +0.5 4,022,521 + +0.5 4,022,521 + +0.3 4,022,522 - -0.6 4,022,522 - +0.3 4,022,522 - -0.5 4,022,558 - -0.1 4,022,683 + HGI48_RS17925 0.80 -0.8 4,022,683 + HGI48_RS17925 0.80 -0.0 4,022,683 + HGI48_RS17925 0.80 -0.8 4,022,684 - HGI48_RS17925 0.80 -2.1 4,022,684 - HGI48_RS17925 0.80 -0.6 4,022,720 + +0.7 4,022,720 + +0.5 4,022,720 + +0.9 4,022,738 + -0.1 4,022,757 + -2.1 4,022,758 - -0.8 4,022,793 + +0.1 4,022,794 - +0.1 4,022,794 - +0.2 4,022,794 - -1.5 4,022,794 - -1.1 4,022,799 + -0.1 4,022,800 - +3.3 4,022,800 - -0.1 4,022,811 + +0.7 4,022,828 + -0.8 4,022,982 + HGI48_RS17930 0.39 +0.3 4,023,129 + HGI48_RS17930 0.75 -0.1 4,023,138 + HGI48_RS17930 0.77 +0.5 4,023,138 + +1.9 4,023,138 + HGI48_RS17930 0.77 -2.3 4,023,139 - HGI48_RS17930 0.78 -0.0 4,023,196 - -0.5 4,023,196 - -0.1 4,023,211 + +0.3 4,023,277 + +0.5 4,023,300 - +0.5 4,023,329 + +0.5 4,023,386 + +0.5 4,026,054 - -0.1 4,026,054 - -0.5 4,026,243 - HGI48_RS17940 0.31 -0.5 4,026,243 - HGI48_RS17940 0.31 +0.0 4,026,243 - HGI48_RS17940 0.31 -0.2 4,026,243 - HGI48_RS17940 0.31 -0.8 4,026,243 - HGI48_RS17940 0.31 -0.9 4,026,255 - HGI48_RS17940 0.33 +0.7 4,026,299 - HGI48_RS17940 0.42 -1.6 4,026,329 - HGI48_RS17940 0.48 +0.2 4,026,418 - HGI48_RS17940 0.66 -0.1 4,026,418 - HGI48_RS17940 0.66 +0.1 4,026,554 - -0.1
Or see this region's nucleotide sequence