Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HGI48_RS17345 and HGI48_RS17350 are separated by 33 nucleotides HGI48_RS17350 and HGI48_RS17355 are separated by 43 nucleotides HGI48_RS17355 and HGI48_RS17360 overlap by 4 nucleotides HGI48_RS17360 and HGI48_RS17365 are separated by 46 nucleotides HGI48_RS17365 and HGI48_RS17370 overlap by 8 nucleotides
HGI48_RS17345: HGI48_RS17345 - YggS family pyridoxal phosphate-dependent enzyme, at 3,885,013 to 3,885,747
_RS17345
HGI48_RS17350: HGI48_RS17350 - pyrroline-5-carboxylate reductase, at 3,885,781 to 3,886,602
_RS17350
HGI48_RS17355: HGI48_RS17355 - YggT family protein, at 3,886,646 to 3,887,200
_RS17355
HGI48_RS17360: HGI48_RS17360 - YggU family protein, at 3,887,197 to 3,887,487
_RS17360
HGI48_RS17365: HGI48_RS17365 - XTP/dITP diphosphatase, at 3,887,534 to 3,888,127
_RS17365
HGI48_RS17370: HGI48_RS17370 - radical SAM family heme chaperone HemW, at 3,888,120 to 3,889,259
_RS17370
Position (kb)
3886
3887
3888 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3885.745 kb on + strand at 3885.745 kb on + strand at 3885.746 kb on - strand at 3886.333 kb on - strand, within HGI48_RS17350 at 3886.651 kb on + strand at 3886.689 kb on - strand at 3886.689 kb on - strand at 3886.692 kb on + strand at 3886.692 kb on + strand at 3886.693 kb on - strand at 3886.742 kb on + strand, within HGI48_RS17355 at 3886.743 kb on - strand, within HGI48_RS17355 at 3886.743 kb on - strand, within HGI48_RS17355 at 3887.039 kb on + strand, within HGI48_RS17355 at 3887.040 kb on - strand, within HGI48_RS17355 at 3887.040 kb on - strand, within HGI48_RS17355 at 3887.040 kb on - strand, within HGI48_RS17355 at 3887.040 kb on - strand, within HGI48_RS17355 at 3887.144 kb on + strand, within HGI48_RS17355 at 3887.145 kb on - strand at 3887.156 kb on + strand at 3887.156 kb on + strand at 3887.157 kb on - strand at 3887.157 kb on - strand at 3887.242 kb on + strand, within HGI48_RS17360 at 3887.242 kb on + strand, within HGI48_RS17360 at 3887.242 kb on + strand at 3887.242 kb on + strand, within HGI48_RS17360 at 3887.242 kb on + strand at 3887.242 kb on + strand, within HGI48_RS17360 at 3887.243 kb on - strand, within HGI48_RS17360 at 3887.243 kb on - strand, within HGI48_RS17360 at 3887.245 kb on - strand, within HGI48_RS17360 at 3887.245 kb on - strand, within HGI48_RS17360 at 3887.245 kb on - strand, within HGI48_RS17360 at 3887.298 kb on + strand, within HGI48_RS17360 at 3887.298 kb on + strand, within HGI48_RS17360 at 3887.298 kb on + strand, within HGI48_RS17360 at 3887.299 kb on - strand, within HGI48_RS17360 at 3887.433 kb on + strand, within HGI48_RS17360 at 3887.433 kb on + strand, within HGI48_RS17360 at 3887.434 kb on - strand, within HGI48_RS17360 at 3887.434 kb on - strand, within HGI48_RS17360 at 3887.491 kb on + strand at 3887.491 kb on + strand at 3887.492 kb on - strand at 3887.492 kb on - strand at 3887.492 kb on - strand at 3887.492 kb on - strand at 3887.492 kb on - strand at 3887.502 kb on + strand at 3887.502 kb on + strand at 3887.503 kb on - strand at 3887.506 kb on + strand at 3887.507 kb on - strand at 3887.569 kb on + strand at 3887.783 kb on + strand, within HGI48_RS17365 at 3887.783 kb on + strand, within HGI48_RS17365 at 3887.895 kb on + strand, within HGI48_RS17365 at 3887.903 kb on + strand, within HGI48_RS17365 at 3887.903 kb on + strand, within HGI48_RS17365 at 3887.903 kb on + strand, within HGI48_RS17365 at 3887.904 kb on - strand, within HGI48_RS17365 at 3887.904 kb on - strand, within HGI48_RS17365 at 3887.904 kb on - strand, within HGI48_RS17365 at 3887.904 kb on - strand at 3887.904 kb on - strand, within HGI48_RS17365 at 3888.125 kb on + strand at 3888.125 kb on + strand at 3888.125 kb on + strand at 3888.125 kb on + strand at 3888.126 kb on - strand at 3888.126 kb on - strand at 3888.126 kb on - strand at 3888.126 kb on - strand at 3888.126 kb on - strand at 3888.140 kb on + strand at 3888.141 kb on - strand at 3888.147 kb on + strand at 3888.147 kb on + strand at 3888.148 kb on - strand at 3888.184 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 3,885,745 + +0.0 3,885,745 + -0.3 3,885,746 - +1.1 3,886,333 - HGI48_RS17350 0.67 -0.5 3,886,651 + +2.1 3,886,689 - +0.6 3,886,689 - -0.9 3,886,692 + -1.5 3,886,692 + -0.8 3,886,693 - -1.7 3,886,742 + HGI48_RS17355 0.17 -1.1 3,886,743 - HGI48_RS17355 0.17 -0.1 3,886,743 - HGI48_RS17355 0.17 -1.5 3,887,039 + HGI48_RS17355 0.71 -0.3 3,887,040 - HGI48_RS17355 0.71 +0.1 3,887,040 - HGI48_RS17355 0.71 -2.5 3,887,040 - HGI48_RS17355 0.71 -2.1 3,887,040 - HGI48_RS17355 0.71 -0.3 3,887,144 + HGI48_RS17355 0.90 -0.8 3,887,145 - -0.7 3,887,156 + -0.5 3,887,156 + +0.5 3,887,157 - +0.5 3,887,157 - +0.1 3,887,242 + HGI48_RS17360 0.15 -2.5 3,887,242 + HGI48_RS17360 0.15 -0.7 3,887,242 + +1.1 3,887,242 + HGI48_RS17360 0.15 -0.2 3,887,242 + -1.1 3,887,242 + HGI48_RS17360 0.15 -0.8 3,887,243 - HGI48_RS17360 0.16 -0.4 3,887,243 - HGI48_RS17360 0.16 -1.2 3,887,245 - HGI48_RS17360 0.16 +0.4 3,887,245 - HGI48_RS17360 0.16 -0.7 3,887,245 - HGI48_RS17360 0.16 -0.3 3,887,298 + HGI48_RS17360 0.35 -0.1 3,887,298 + HGI48_RS17360 0.35 -0.0 3,887,298 + HGI48_RS17360 0.35 +1.8 3,887,299 - HGI48_RS17360 0.35 +0.1 3,887,433 + HGI48_RS17360 0.81 +0.3 3,887,433 + HGI48_RS17360 0.81 +0.5 3,887,434 - HGI48_RS17360 0.81 +0.3 3,887,434 - HGI48_RS17360 0.81 +0.1 3,887,491 + -0.8 3,887,491 + -1.1 3,887,492 - -0.3 3,887,492 - -1.1 3,887,492 - -1.3 3,887,492 - +0.2 3,887,492 - +0.2 3,887,502 + -1.1 3,887,502 + -0.7 3,887,503 - -1.1 3,887,506 + -0.7 3,887,507 - +0.5 3,887,569 + -1.3 3,887,783 + HGI48_RS17365 0.42 +0.4 3,887,783 + HGI48_RS17365 0.42 +0.2 3,887,895 + HGI48_RS17365 0.61 -1.5 3,887,903 + HGI48_RS17365 0.62 -0.8 3,887,903 + HGI48_RS17365 0.62 +1.9 3,887,903 + HGI48_RS17365 0.62 +1.0 3,887,904 - HGI48_RS17365 0.62 -1.3 3,887,904 - HGI48_RS17365 0.62 +0.4 3,887,904 - HGI48_RS17365 0.62 -1.3 3,887,904 - +0.9 3,887,904 - HGI48_RS17365 0.62 +1.0 3,888,125 + -0.5 3,888,125 + -0.3 3,888,125 + -0.5 3,888,125 + +1.1 3,888,126 - -2.4 3,888,126 - +2.2 3,888,126 - -0.8 3,888,126 - -3.0 3,888,126 - -0.3 3,888,140 + -0.5 3,888,141 - -0.5 3,888,147 + -0.8 3,888,147 + +0.1 3,888,148 - -1.8 3,888,184 - -0.5
Or see this region's nucleotide sequence