Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HGI48_RS09715 and HGI48_RS09720 overlap by 7 nucleotides HGI48_RS09720 and HGI48_RS09725 overlap by 19 nucleotides HGI48_RS09725 and HGI48_RS09730 are separated by 6 nucleotides HGI48_RS09730 and HGI48_RS09735 overlap by 1 nucleotides
HGI48_RS09715: HGI48_RS09715 - bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE, at 2,099,049 to 2,099,663
_RS09715
HGI48_RS09720: HGI48_RS09720 - imidazole glycerol phosphate synthase subunit HisF, at 2,099,657 to 2,100,433
_RS09720
HGI48_RS09725: HGI48_RS09725 - 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino]imidazole-4- carboxamide isomerase, at 2,100,415 to 2,101,152
_RS09725
HGI48_RS09730: HGI48_RS09730 - imidazole glycerol phosphate synthase subunit HisH, at 2,101,159 to 2,101,749
_RS09730
HGI48_RS09735: HGI48_RS09735 - bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB, at 2,101,749 to 2,102,816
_RS09735
Position (kb)
2100
2101
2102 Strain fitness (log2 ratio)
-1
0
1 at 2099.517 kb on - strand, within HGI48_RS09715 at 2099.557 kb on + strand, within HGI48_RS09715 at 2099.595 kb on + strand, within HGI48_RS09715 at 2099.690 kb on - strand at 2099.846 kb on + strand, within HGI48_RS09720 at 2100.007 kb on + strand, within HGI48_RS09720 at 2100.593 kb on + strand, within HGI48_RS09725 at 2101.232 kb on + strand, within HGI48_RS09730 at 2101.438 kb on + strand, within HGI48_RS09730 at 2101.697 kb on + strand at 2101.698 kb on - strand at 2101.975 kb on + strand, within HGI48_RS09735 at 2101.975 kb on + strand, within HGI48_RS09735 at 2101.975 kb on + strand at 2102.041 kb on - strand, within HGI48_RS09735
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 2,099,517 - HGI48_RS09715 0.76 +0.8 2,099,557 + HGI48_RS09715 0.83 -1.0 2,099,595 + HGI48_RS09715 0.89 -0.7 2,099,690 - -0.8 2,099,846 + HGI48_RS09720 0.24 -0.9 2,100,007 + HGI48_RS09720 0.45 -1.2 2,100,593 + HGI48_RS09725 0.24 +0.4 2,101,232 + HGI48_RS09730 0.12 -0.9 2,101,438 + HGI48_RS09730 0.47 -0.8 2,101,697 + -0.3 2,101,698 - +0.1 2,101,975 + HGI48_RS09735 0.21 -1.4 2,101,975 + HGI48_RS09735 0.21 -0.9 2,101,975 + +1.1 2,102,041 - HGI48_RS09735 0.27 +0.6
Or see this region's nucleotide sequence