Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HGI48_RS05180 and HGI48_RS05185 overlap by 4 nucleotides HGI48_RS05185 and HGI48_RS05190 are separated by 75 nucleotides HGI48_RS05190 and HGI48_RS05195 are separated by 12 nucleotides
HGI48_RS05180: HGI48_RS05180 - lipid-A-disaccharide synthase, at 1,085,793 to 1,086,941
_RS05180
HGI48_RS05185: HGI48_RS05185 - ribonuclease HII, at 1,086,938 to 1,087,534
_RS05185
HGI48_RS05190: HGI48_RS05190 - DNA polymerase III subunit alpha, at 1,087,610 to 1,091,092
_RS05190
HGI48_RS05195: HGI48_RS05195 - acetyl-CoA carboxylase carboxyl transferase subunit alpha, at 1,091,105 to 1,092,064
_RS05195
Position (kb)
1087
1088
1089
1090
1091
1092 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1086.973 kb on + strand at 1086.974 kb on - strand at 1086.974 kb on - strand at 1086.974 kb on - strand at 1086.974 kb on - strand at 1086.993 kb on + strand at 1086.993 kb on + strand at 1086.994 kb on - strand at 1087.184 kb on - strand at 1087.445 kb on + strand, within HGI48_RS05185 at 1087.445 kb on + strand at 1087.445 kb on + strand at 1087.537 kb on + strand at 1087.554 kb on + strand at 1087.554 kb on + strand at 1087.554 kb on + strand at 1087.554 kb on + strand at 1087.554 kb on + strand at 1087.554 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 1,086,973 + -1.6 1,086,974 - +1.1 1,086,974 - -1.5 1,086,974 - -0.6 1,086,974 - -0.2 1,086,993 + -0.1 1,086,993 + +2.0 1,086,994 - +0.2 1,087,184 - +1.4 1,087,445 + HGI48_RS05185 0.85 -2.6 1,087,445 + -0.6 1,087,445 + +0.8 1,087,537 + +0.1 1,087,554 + -0.5 1,087,554 + -2.2 1,087,554 + +0.1 1,087,554 + +1.2 1,087,554 + +0.0 1,087,554 + -0.1
Or see this region's nucleotide sequence