Experiment: M9+glycerol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DDA3937_RS13470 and DDA3937_RS13475 are separated by 12 nucleotides DDA3937_RS13475 and DDA3937_RS13480 are separated by 33 nucleotides DDA3937_RS13480 and DDA3937_RS13485 are separated by 166 nucleotides DDA3937_RS13485 and DDA3937_RS13490 are separated by 144 nucleotides
DDA3937_RS13470: DDA3937_RS13470 - flagellar hook assembly protein FlgD, at 3,071,764 to 3,072,435
_RS13470
DDA3937_RS13475: DDA3937_RS13475 - flagellar basal body rod protein FlgC, at 3,072,448 to 3,072,852
_RS13475
DDA3937_RS13480: DDA3937_RS13480 - flagellar basal body rod protein FlgB, at 3,072,886 to 3,073,305
_RS13480
DDA3937_RS13485: DDA3937_RS13485 - flagellar basal body P-ring formation protein FlgA, at 3,073,472 to 3,074,140
_RS13485
DDA3937_RS13490: DDA3937_RS13490 - anti-sigma-28 factor FlgM, at 3,074,285 to 3,074,584
_RS13490
Position (kb)
3072
3073
3074 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5
6
7
8 at 3071.922 kb on + strand, within DDA3937_RS13470 at 3071.922 kb on + strand, within DDA3937_RS13470 at 3071.922 kb on + strand, within DDA3937_RS13470 at 3071.922 kb on + strand, within DDA3937_RS13470 at 3071.922 kb on + strand, within DDA3937_RS13470 at 3071.922 kb on + strand, within DDA3937_RS13470 at 3071.923 kb on - strand, within DDA3937_RS13470 at 3071.923 kb on - strand, within DDA3937_RS13470 at 3071.923 kb on - strand, within DDA3937_RS13470 at 3071.923 kb on - strand, within DDA3937_RS13470 at 3071.923 kb on - strand, within DDA3937_RS13470 at 3071.923 kb on - strand, within DDA3937_RS13470 at 3071.923 kb on - strand, within DDA3937_RS13470 at 3071.923 kb on - strand, within DDA3937_RS13470 at 3071.923 kb on - strand, within DDA3937_RS13470 at 3071.923 kb on - strand at 3071.923 kb on - strand, within DDA3937_RS13470 at 3071.924 kb on + strand, within DDA3937_RS13470 at 3071.924 kb on + strand, within DDA3937_RS13470 at 3071.924 kb on + strand, within DDA3937_RS13470 at 3071.925 kb on - strand, within DDA3937_RS13470 at 3071.925 kb on - strand, within DDA3937_RS13470 at 3071.975 kb on + strand, within DDA3937_RS13470 at 3071.976 kb on - strand, within DDA3937_RS13470 at 3071.976 kb on - strand, within DDA3937_RS13470 at 3071.976 kb on - strand, within DDA3937_RS13470 at 3071.976 kb on - strand, within DDA3937_RS13470 at 3071.976 kb on - strand, within DDA3937_RS13470 at 3072.012 kb on + strand, within DDA3937_RS13470 at 3072.013 kb on - strand at 3072.022 kb on + strand at 3072.022 kb on + strand, within DDA3937_RS13470 at 3072.022 kb on + strand, within DDA3937_RS13470 at 3072.023 kb on - strand, within DDA3937_RS13470 at 3072.023 kb on - strand, within DDA3937_RS13470 at 3072.196 kb on - strand, within DDA3937_RS13470 at 3072.217 kb on - strand, within DDA3937_RS13470 at 3072.247 kb on + strand, within DDA3937_RS13470 at 3072.247 kb on + strand, within DDA3937_RS13470 at 3072.248 kb on - strand, within DDA3937_RS13470 at 3072.339 kb on + strand, within DDA3937_RS13470 at 3072.340 kb on - strand, within DDA3937_RS13470 at 3072.340 kb on - strand, within DDA3937_RS13470 at 3072.340 kb on - strand, within DDA3937_RS13470 at 3072.340 kb on - strand, within DDA3937_RS13470 at 3072.340 kb on - strand, within DDA3937_RS13470 at 3072.347 kb on - strand, within DDA3937_RS13470 at 3072.361 kb on - strand, within DDA3937_RS13470 at 3072.361 kb on - strand, within DDA3937_RS13470 at 3072.361 kb on - strand, within DDA3937_RS13470 at 3072.361 kb on - strand, within DDA3937_RS13470 at 3072.372 kb on + strand at 3072.399 kb on + strand at 3072.400 kb on - strand at 3072.400 kb on - strand at 3072.429 kb on + strand at 3072.463 kb on + strand at 3072.493 kb on - strand, within DDA3937_RS13475 at 3072.500 kb on - strand, within DDA3937_RS13475 at 3072.518 kb on + strand, within DDA3937_RS13475 at 3072.518 kb on + strand, within DDA3937_RS13475 at 3072.519 kb on - strand, within DDA3937_RS13475 at 3072.519 kb on - strand, within DDA3937_RS13475 at 3072.519 kb on - strand, within DDA3937_RS13475 at 3072.584 kb on + strand, within DDA3937_RS13475 at 3072.584 kb on + strand, within DDA3937_RS13475 at 3072.584 kb on + strand, within DDA3937_RS13475 at 3072.584 kb on + strand, within DDA3937_RS13475 at 3072.584 kb on + strand, within DDA3937_RS13475 at 3072.585 kb on - strand, within DDA3937_RS13475 at 3072.620 kb on + strand, within DDA3937_RS13475 at 3072.666 kb on + strand, within DDA3937_RS13475 at 3072.667 kb on - strand, within DDA3937_RS13475 at 3072.667 kb on - strand, within DDA3937_RS13475 at 3072.838 kb on - strand at 3072.838 kb on - strand at 3072.840 kb on + strand at 3072.844 kb on + strand at 3072.844 kb on + strand at 3072.845 kb on - strand at 3072.866 kb on + strand at 3072.866 kb on + strand at 3072.866 kb on + strand at 3072.866 kb on + strand at 3072.866 kb on + strand at 3072.866 kb on + strand at 3072.866 kb on + strand at 3072.866 kb on + strand at 3072.867 kb on - strand at 3072.867 kb on - strand at 3072.867 kb on - strand at 3072.867 kb on - strand at 3072.868 kb on + strand at 3072.868 kb on + strand at 3072.868 kb on + strand at 3072.868 kb on + strand at 3072.868 kb on + strand at 3072.868 kb on + strand at 3072.868 kb on + strand at 3072.868 kb on + strand at 3072.868 kb on + strand at 3072.868 kb on + strand at 3072.868 kb on + strand at 3072.868 kb on + strand at 3072.868 kb on + strand at 3072.868 kb on + strand at 3072.869 kb on - strand at 3072.869 kb on - strand at 3072.869 kb on - strand at 3072.869 kb on - strand at 3072.869 kb on - strand at 3072.887 kb on + strand at 3072.887 kb on + strand at 3072.888 kb on - strand at 3072.908 kb on - strand at 3072.959 kb on + strand, within DDA3937_RS13480 at 3072.959 kb on + strand, within DDA3937_RS13480 at 3072.959 kb on + strand, within DDA3937_RS13480 at 3072.960 kb on - strand, within DDA3937_RS13480 at 3072.960 kb on - strand, within DDA3937_RS13480 at 3072.960 kb on - strand, within DDA3937_RS13480 at 3072.987 kb on + strand, within DDA3937_RS13480 at 3073.003 kb on - strand, within DDA3937_RS13480 at 3073.072 kb on + strand, within DDA3937_RS13480 at 3073.089 kb on + strand, within DDA3937_RS13480 at 3073.089 kb on + strand, within DDA3937_RS13480 at 3073.090 kb on - strand, within DDA3937_RS13480 at 3073.090 kb on - strand, within DDA3937_RS13480 at 3073.193 kb on + strand, within DDA3937_RS13480 at 3073.297 kb on - strand at 3073.328 kb on + strand at 3073.337 kb on + strand at 3073.337 kb on + strand at 3073.337 kb on + strand at 3073.342 kb on + strand at 3073.374 kb on + strand at 3073.374 kb on + strand at 3073.380 kb on + strand at 3073.380 kb on + strand at 3073.381 kb on - strand at 3073.393 kb on + strand at 3073.397 kb on + strand at 3073.398 kb on - strand at 3073.398 kb on - strand at 3073.398 kb on - strand at 3073.398 kb on - strand at 3073.398 kb on - strand at 3073.411 kb on + strand at 3073.411 kb on + strand at 3073.411 kb on + strand at 3073.412 kb on - strand at 3073.412 kb on - strand at 3073.419 kb on + strand at 3073.420 kb on - strand at 3073.430 kb on + strand at 3073.431 kb on - strand at 3073.431 kb on - strand at 3073.431 kb on - strand at 3073.433 kb on + strand at 3073.433 kb on + strand at 3073.433 kb on + strand at 3073.465 kb on - strand at 3073.465 kb on - strand at 3073.465 kb on - strand at 3073.465 kb on - strand at 3073.465 kb on - strand at 3073.465 kb on - strand at 3073.490 kb on + strand at 3073.494 kb on + strand at 3073.494 kb on + strand at 3073.494 kb on + strand at 3073.494 kb on + strand at 3073.495 kb on - strand at 3073.495 kb on - strand at 3073.495 kb on - strand at 3073.495 kb on - strand at 3073.495 kb on - strand at 3073.495 kb on - strand at 3073.588 kb on + strand, within DDA3937_RS13485 at 3073.609 kb on + strand, within DDA3937_RS13485 at 3073.609 kb on + strand, within DDA3937_RS13485 at 3073.609 kb on + strand, within DDA3937_RS13485 at 3073.609 kb on + strand, within DDA3937_RS13485 at 3073.610 kb on - strand, within DDA3937_RS13485 at 3073.610 kb on - strand, within DDA3937_RS13485 at 3073.752 kb on + strand, within DDA3937_RS13485 at 3073.752 kb on + strand, within DDA3937_RS13485 at 3073.753 kb on - strand, within DDA3937_RS13485 at 3073.769 kb on + strand, within DDA3937_RS13485 at 3073.769 kb on + strand, within DDA3937_RS13485 at 3073.769 kb on + strand, within DDA3937_RS13485 at 3073.769 kb on + strand, within DDA3937_RS13485 at 3073.769 kb on + strand, within DDA3937_RS13485 at 3073.769 kb on + strand, within DDA3937_RS13485 at 3073.769 kb on + strand, within DDA3937_RS13485 at 3073.769 kb on + strand, within DDA3937_RS13485 at 3073.769 kb on + strand, within DDA3937_RS13485 at 3073.769 kb on + strand, within DDA3937_RS13485 at 3073.770 kb on - strand, within DDA3937_RS13485 at 3073.770 kb on - strand, within DDA3937_RS13485 at 3073.770 kb on - strand, within DDA3937_RS13485 at 3073.770 kb on - strand, within DDA3937_RS13485 at 3073.770 kb on - strand, within DDA3937_RS13485 at 3073.770 kb on - strand, within DDA3937_RS13485 at 3074.021 kb on - strand, within DDA3937_RS13485 at 3074.095 kb on - strand at 3074.123 kb on - strand at 3074.138 kb on + strand at 3074.139 kb on - strand at 3074.147 kb on - strand at 3074.151 kb on + strand at 3074.151 kb on + strand at 3074.151 kb on + strand at 3074.151 kb on + strand at 3074.171 kb on - strand at 3074.177 kb on + strand at 3074.177 kb on + strand at 3074.177 kb on + strand at 3074.177 kb on + strand at 3074.177 kb on + strand at 3074.177 kb on + strand at 3074.177 kb on + strand at 3074.177 kb on + strand at 3074.177 kb on + strand at 3074.178 kb on - strand at 3074.178 kb on - strand at 3074.181 kb on + strand at 3074.181 kb on + strand at 3074.181 kb on + strand at 3074.181 kb on + strand at 3074.181 kb on + strand at 3074.182 kb on - strand at 3074.182 kb on - strand at 3074.194 kb on + strand at 3074.194 kb on + strand at 3074.207 kb on + strand at 3074.207 kb on + strand at 3074.281 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9+glycerol remove 3,071,922 + DDA3937_RS13470 0.24 +2.7 3,071,922 + DDA3937_RS13470 0.24 +4.1 3,071,922 + DDA3937_RS13470 0.24 +4.2 3,071,922 + DDA3937_RS13470 0.24 +1.7 3,071,922 + DDA3937_RS13470 0.24 +3.1 3,071,922 + DDA3937_RS13470 0.24 +2.3 3,071,923 - DDA3937_RS13470 0.24 +4.5 3,071,923 - DDA3937_RS13470 0.24 +1.9 3,071,923 - DDA3937_RS13470 0.24 +4.2 3,071,923 - DDA3937_RS13470 0.24 +2.6 3,071,923 - DDA3937_RS13470 0.24 +4.6 3,071,923 - DDA3937_RS13470 0.24 +4.1 3,071,923 - DDA3937_RS13470 0.24 -0.3 3,071,923 - DDA3937_RS13470 0.24 +3.6 3,071,923 - DDA3937_RS13470 0.24 +3.5 3,071,923 - +2.5 3,071,923 - DDA3937_RS13470 0.24 +0.8 3,071,924 + DDA3937_RS13470 0.24 +2.8 3,071,924 + DDA3937_RS13470 0.24 +3.2 3,071,924 + DDA3937_RS13470 0.24 +0.7 3,071,925 - DDA3937_RS13470 0.24 +6.1 3,071,925 - DDA3937_RS13470 0.24 +0.1 3,071,975 + DDA3937_RS13470 0.31 +1.4 3,071,976 - DDA3937_RS13470 0.32 +4.6 3,071,976 - DDA3937_RS13470 0.32 +4.1 3,071,976 - DDA3937_RS13470 0.32 +1.2 3,071,976 - DDA3937_RS13470 0.32 +4.0 3,071,976 - DDA3937_RS13470 0.32 +2.1 3,072,012 + DDA3937_RS13470 0.37 +3.4 3,072,013 - +3.8 3,072,022 + -0.7 3,072,022 + DDA3937_RS13470 0.38 +4.6 3,072,022 + DDA3937_RS13470 0.38 +3.1 3,072,023 - DDA3937_RS13470 0.39 +2.6 3,072,023 - DDA3937_RS13470 0.39 +2.8 3,072,196 - DDA3937_RS13470 0.64 +2.9 3,072,217 - DDA3937_RS13470 0.67 +4.3 3,072,247 + DDA3937_RS13470 0.72 +4.0 3,072,247 + DDA3937_RS13470 0.72 +4.8 3,072,248 - DDA3937_RS13470 0.72 +2.8 3,072,339 + DDA3937_RS13470 0.86 +1.3 3,072,340 - DDA3937_RS13470 0.86 +2.9 3,072,340 - DDA3937_RS13470 0.86 +3.2 3,072,340 - DDA3937_RS13470 0.86 +2.9 3,072,340 - DDA3937_RS13470 0.86 +2.4 3,072,340 - DDA3937_RS13470 0.86 +6.5 3,072,347 - DDA3937_RS13470 0.87 +2.3 3,072,361 - DDA3937_RS13470 0.89 +1.6 3,072,361 - DDA3937_RS13470 0.89 +3.9 3,072,361 - DDA3937_RS13470 0.89 +6.8 3,072,361 - DDA3937_RS13470 0.89 +5.3 3,072,372 + +1.6 3,072,399 + +2.7 3,072,400 - +4.2 3,072,400 - +1.9 3,072,429 + +3.1 3,072,463 + +2.2 3,072,493 - DDA3937_RS13475 0.11 +3.9 3,072,500 - DDA3937_RS13475 0.13 +3.8 3,072,518 + DDA3937_RS13475 0.17 +3.4 3,072,518 + DDA3937_RS13475 0.17 +2.2 3,072,519 - DDA3937_RS13475 0.18 +3.6 3,072,519 - DDA3937_RS13475 0.18 +2.3 3,072,519 - DDA3937_RS13475 0.18 +4.4 3,072,584 + DDA3937_RS13475 0.34 +0.8 3,072,584 + DDA3937_RS13475 0.34 -1.3 3,072,584 + DDA3937_RS13475 0.34 +3.5 3,072,584 + DDA3937_RS13475 0.34 +1.4 3,072,584 + DDA3937_RS13475 0.34 +1.7 3,072,585 - DDA3937_RS13475 0.34 +4.5 3,072,620 + DDA3937_RS13475 0.42 +1.5 3,072,666 + DDA3937_RS13475 0.54 +1.5 3,072,667 - DDA3937_RS13475 0.54 -0.3 3,072,667 - DDA3937_RS13475 0.54 +2.5 3,072,838 - +1.6 3,072,838 - +3.1 3,072,840 + +3.0 3,072,844 + +3.0 3,072,844 + +2.9 3,072,845 - +1.2 3,072,866 + +1.6 3,072,866 + +1.9 3,072,866 + +2.2 3,072,866 + +2.3 3,072,866 + +1.5 3,072,866 + +3.0 3,072,866 + +1.3 3,072,866 + +2.4 3,072,867 - +2.6 3,072,867 - +2.6 3,072,867 - +2.4 3,072,867 - +2.7 3,072,868 + +4.6 3,072,868 + +3.0 3,072,868 + +5.1 3,072,868 + +2.8 3,072,868 + +2.6 3,072,868 + +2.4 3,072,868 + +3.0 3,072,868 + +4.1 3,072,868 + +0.7 3,072,868 + -1.6 3,072,868 + +2.6 3,072,868 + +4.2 3,072,868 + +2.3 3,072,868 + +0.3 3,072,869 - +2.5 3,072,869 - +1.0 3,072,869 - +2.4 3,072,869 - -0.0 3,072,869 - -2.7 3,072,887 + +2.4 3,072,887 + +5.5 3,072,888 - -2.4 3,072,908 - +2.3 3,072,959 + DDA3937_RS13480 0.17 +3.3 3,072,959 + DDA3937_RS13480 0.17 +2.1 3,072,959 + DDA3937_RS13480 0.17 +5.1 3,072,960 - DDA3937_RS13480 0.18 +3.2 3,072,960 - DDA3937_RS13480 0.18 +4.2 3,072,960 - DDA3937_RS13480 0.18 +2.8 3,072,987 + DDA3937_RS13480 0.24 +1.8 3,073,003 - DDA3937_RS13480 0.28 +3.6 3,073,072 + DDA3937_RS13480 0.44 +3.3 3,073,089 + DDA3937_RS13480 0.48 +4.1 3,073,089 + DDA3937_RS13480 0.48 +3.6 3,073,090 - DDA3937_RS13480 0.49 +2.5 3,073,090 - DDA3937_RS13480 0.49 +1.5 3,073,193 + DDA3937_RS13480 0.73 +4.4 3,073,297 - +2.7 3,073,328 + +2.3 3,073,337 + +3.0 3,073,337 + +3.6 3,073,337 + +0.7 3,073,342 + +2.5 3,073,374 + +2.5 3,073,374 + +5.8 3,073,380 + +2.4 3,073,380 + +2.3 3,073,381 - -0.9 3,073,393 + +0.8 3,073,397 + -1.7 3,073,398 - -0.3 3,073,398 - -0.7 3,073,398 - -2.3 3,073,398 - -0.3 3,073,398 - +1.2 3,073,411 + -1.7 3,073,411 + +1.5 3,073,411 + -1.3 3,073,412 - -0.1 3,073,412 - -1.3 3,073,419 + +0.1 3,073,420 - -1.3 3,073,430 + -1.3 3,073,431 - +3.6 3,073,431 - +1.5 3,073,431 - +2.3 3,073,433 + +0.2 3,073,433 + -2.6 3,073,433 + -1.1 3,073,465 - +4.3 3,073,465 - +5.9 3,073,465 - +2.9 3,073,465 - +3.0 3,073,465 - +2.0 3,073,465 - +4.1 3,073,490 + +1.8 3,073,494 + +2.7 3,073,494 + +3.1 3,073,494 + +1.6 3,073,494 + +2.8 3,073,495 - +1.6 3,073,495 - +2.8 3,073,495 - +8.6 3,073,495 - +3.5 3,073,495 - +2.6 3,073,495 - +4.0 3,073,588 + DDA3937_RS13485 0.17 +3.4 3,073,609 + DDA3937_RS13485 0.20 +2.8 3,073,609 + DDA3937_RS13485 0.20 +1.4 3,073,609 + DDA3937_RS13485 0.20 +1.7 3,073,609 + DDA3937_RS13485 0.20 +3.4 3,073,610 - DDA3937_RS13485 0.21 -1.6 3,073,610 - DDA3937_RS13485 0.21 +3.6 3,073,752 + DDA3937_RS13485 0.42 +2.7 3,073,752 + DDA3937_RS13485 0.42 +0.5 3,073,753 - DDA3937_RS13485 0.42 +1.9 3,073,769 + DDA3937_RS13485 0.44 +3.0 3,073,769 + DDA3937_RS13485 0.44 +2.9 3,073,769 + DDA3937_RS13485 0.44 +3.6 3,073,769 + DDA3937_RS13485 0.44 +2.1 3,073,769 + DDA3937_RS13485 0.44 +1.7 3,073,769 + DDA3937_RS13485 0.44 +2.2 3,073,769 + DDA3937_RS13485 0.44 +3.0 3,073,769 + DDA3937_RS13485 0.44 +4.5 3,073,769 + DDA3937_RS13485 0.44 -0.7 3,073,769 + DDA3937_RS13485 0.44 +1.8 3,073,770 - DDA3937_RS13485 0.45 +3.1 3,073,770 - DDA3937_RS13485 0.45 +4.2 3,073,770 - DDA3937_RS13485 0.45 +2.4 3,073,770 - DDA3937_RS13485 0.45 +4.6 3,073,770 - DDA3937_RS13485 0.45 +4.6 3,073,770 - DDA3937_RS13485 0.45 +3.9 3,074,021 - DDA3937_RS13485 0.82 +3.9 3,074,095 - +0.7 3,074,123 - +0.9 3,074,138 + -1.6 3,074,139 - -1.5 3,074,147 - -2.1 3,074,151 + -0.8 3,074,151 + -1.2 3,074,151 + -1.1 3,074,151 + -1.9 3,074,171 - -1.3 3,074,177 + -1.0 3,074,177 + -2.5 3,074,177 + -2.7 3,074,177 + -2.5 3,074,177 + +1.0 3,074,177 + -0.1 3,074,177 + -0.6 3,074,177 + -0.8 3,074,177 + -1.2 3,074,178 - -1.1 3,074,178 - -0.9 3,074,181 + -1.3 3,074,181 + -0.3 3,074,181 + +0.1 3,074,181 + -0.4 3,074,181 + -2.0 3,074,182 - -0.3 3,074,182 - -1.1 3,074,194 + +0.2 3,074,194 + -0.9 3,074,207 + -1.1 3,074,207 + -0.1 3,074,281 + -0.3
Or see this region's nucleotide sequence