Experiment: M9+glycerol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DDA3937_RS09700 and DDA3937_RS09705 are separated by 248 nucleotides DDA3937_RS09705 and DDA3937_RS09710 overlap by 7 nucleotides DDA3937_RS09710 and DDA3937_RS09715 overlap by 19 nucleotides
DDA3937_RS09700: DDA3937_RS09700 - hypothetical protein, at 2,190,991 to 2,191,626
_RS09700
DDA3937_RS09705: DDA3937_RS09705 - bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE, at 2,191,875 to 2,192,489
_RS09705
DDA3937_RS09710: DDA3937_RS09710 - imidazole glycerol phosphate synthase subunit HisF, at 2,192,483 to 2,193,259
_RS09710
DDA3937_RS09715: DDA3937_RS09715 - 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino]imidazole-4- carboxamide isomerase, at 2,193,241 to 2,193,978
_RS09715
Position (kb)
2191
2192
2193 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 2190.882 kb on + strand at 2190.883 kb on - strand at 2190.898 kb on - strand at 2190.898 kb on - strand at 2190.898 kb on - strand at 2190.905 kb on + strand at 2190.906 kb on - strand at 2190.906 kb on - strand at 2190.927 kb on - strand at 2190.939 kb on + strand at 2190.939 kb on + strand at 2190.944 kb on - strand at 2190.944 kb on - strand at 2190.944 kb on - strand at 2191.011 kb on - strand at 2191.011 kb on - strand at 2191.065 kb on + strand, within DDA3937_RS09700 at 2191.065 kb on + strand, within DDA3937_RS09700 at 2191.066 kb on - strand, within DDA3937_RS09700 at 2191.069 kb on + strand, within DDA3937_RS09700 at 2191.083 kb on + strand, within DDA3937_RS09700 at 2191.083 kb on + strand, within DDA3937_RS09700 at 2191.083 kb on + strand, within DDA3937_RS09700 at 2191.084 kb on - strand, within DDA3937_RS09700 at 2191.084 kb on - strand, within DDA3937_RS09700 at 2191.086 kb on - strand, within DDA3937_RS09700 at 2191.086 kb on - strand, within DDA3937_RS09700 at 2191.086 kb on - strand, within DDA3937_RS09700 at 2191.170 kb on + strand, within DDA3937_RS09700 at 2191.204 kb on + strand, within DDA3937_RS09700 at 2191.209 kb on + strand, within DDA3937_RS09700 at 2191.250 kb on + strand, within DDA3937_RS09700 at 2191.251 kb on - strand at 2191.254 kb on + strand, within DDA3937_RS09700 at 2191.254 kb on + strand, within DDA3937_RS09700 at 2191.262 kb on + strand, within DDA3937_RS09700 at 2191.266 kb on + strand, within DDA3937_RS09700 at 2191.266 kb on + strand, within DDA3937_RS09700 at 2191.266 kb on + strand, within DDA3937_RS09700 at 2191.267 kb on - strand, within DDA3937_RS09700 at 2191.267 kb on - strand, within DDA3937_RS09700 at 2191.267 kb on - strand, within DDA3937_RS09700 at 2191.269 kb on - strand, within DDA3937_RS09700 at 2191.278 kb on + strand at 2191.278 kb on + strand at 2191.279 kb on - strand, within DDA3937_RS09700 at 2191.279 kb on - strand, within DDA3937_RS09700 at 2191.279 kb on - strand, within DDA3937_RS09700 at 2191.279 kb on - strand, within DDA3937_RS09700 at 2191.279 kb on - strand, within DDA3937_RS09700 at 2191.320 kb on + strand, within DDA3937_RS09700 at 2191.320 kb on + strand, within DDA3937_RS09700 at 2191.320 kb on + strand, within DDA3937_RS09700 at 2191.321 kb on - strand, within DDA3937_RS09700 at 2191.321 kb on - strand, within DDA3937_RS09700 at 2191.321 kb on - strand, within DDA3937_RS09700 at 2191.321 kb on - strand at 2191.322 kb on + strand, within DDA3937_RS09700 at 2191.322 kb on + strand, within DDA3937_RS09700 at 2191.322 kb on + strand, within DDA3937_RS09700 at 2191.322 kb on + strand, within DDA3937_RS09700 at 2191.322 kb on + strand, within DDA3937_RS09700 at 2191.323 kb on - strand, within DDA3937_RS09700 at 2191.323 kb on - strand, within DDA3937_RS09700 at 2191.323 kb on - strand, within DDA3937_RS09700 at 2191.323 kb on - strand, within DDA3937_RS09700 at 2191.346 kb on + strand, within DDA3937_RS09700 at 2191.363 kb on - strand, within DDA3937_RS09700 at 2191.376 kb on + strand, within DDA3937_RS09700 at 2191.376 kb on + strand, within DDA3937_RS09700 at 2191.376 kb on + strand, within DDA3937_RS09700 at 2191.376 kb on + strand, within DDA3937_RS09700 at 2191.377 kb on - strand, within DDA3937_RS09700 at 2191.377 kb on - strand, within DDA3937_RS09700 at 2191.377 kb on - strand, within DDA3937_RS09700 at 2191.377 kb on - strand at 2191.377 kb on - strand, within DDA3937_RS09700 at 2191.377 kb on - strand, within DDA3937_RS09700 at 2191.377 kb on - strand, within DDA3937_RS09700 at 2191.377 kb on - strand, within DDA3937_RS09700 at 2191.377 kb on - strand, within DDA3937_RS09700 at 2191.377 kb on - strand, within DDA3937_RS09700 at 2191.377 kb on - strand, within DDA3937_RS09700 at 2191.377 kb on - strand, within DDA3937_RS09700 at 2191.431 kb on + strand, within DDA3937_RS09700 at 2191.431 kb on + strand, within DDA3937_RS09700 at 2191.431 kb on + strand at 2191.432 kb on - strand, within DDA3937_RS09700 at 2191.463 kb on - strand, within DDA3937_RS09700 at 2191.478 kb on + strand, within DDA3937_RS09700 at 2191.478 kb on + strand, within DDA3937_RS09700 at 2191.479 kb on - strand, within DDA3937_RS09700 at 2191.479 kb on - strand, within DDA3937_RS09700 at 2191.479 kb on - strand, within DDA3937_RS09700 at 2191.497 kb on + strand, within DDA3937_RS09700 at 2191.501 kb on - strand, within DDA3937_RS09700 at 2191.589 kb on - strand at 2191.604 kb on + strand at 2191.604 kb on + strand at 2191.604 kb on + strand at 2191.627 kb on - strand at 2191.656 kb on + strand at 2191.738 kb on - strand at 2191.738 kb on - strand at 2191.944 kb on + strand, within DDA3937_RS09705 at 2191.945 kb on - strand, within DDA3937_RS09705 at 2191.945 kb on - strand, within DDA3937_RS09705 at 2191.957 kb on + strand, within DDA3937_RS09705 at 2191.957 kb on + strand, within DDA3937_RS09705 at 2191.957 kb on + strand, within DDA3937_RS09705 at 2192.075 kb on - strand, within DDA3937_RS09705 at 2192.090 kb on - strand, within DDA3937_RS09705 at 2192.115 kb on - strand, within DDA3937_RS09705 at 2192.300 kb on + strand, within DDA3937_RS09705 at 2192.300 kb on + strand, within DDA3937_RS09705 at 2192.318 kb on + strand, within DDA3937_RS09705 at 2192.319 kb on - strand, within DDA3937_RS09705 at 2192.319 kb on - strand, within DDA3937_RS09705 at 2192.321 kb on + strand, within DDA3937_RS09705 at 2192.321 kb on + strand, within DDA3937_RS09705 at 2192.330 kb on - strand, within DDA3937_RS09705 at 2192.330 kb on - strand, within DDA3937_RS09705 at 2192.383 kb on + strand, within DDA3937_RS09705 at 2192.384 kb on - strand, within DDA3937_RS09705 at 2192.697 kb on + strand, within DDA3937_RS09710 at 2192.698 kb on - strand, within DDA3937_RS09710 at 2192.797 kb on + strand, within DDA3937_RS09710 at 2192.827 kb on - strand, within DDA3937_RS09710 at 2192.827 kb on - strand, within DDA3937_RS09710 at 2192.827 kb on - strand, within DDA3937_RS09710 at 2192.834 kb on - strand, within DDA3937_RS09710 at 2192.869 kb on + strand, within DDA3937_RS09710 at 2192.894 kb on + strand, within DDA3937_RS09710 at 2192.894 kb on + strand at 2192.895 kb on - strand, within DDA3937_RS09710 at 2193.112 kb on - strand, within DDA3937_RS09710 at 2193.112 kb on - strand, within DDA3937_RS09710 at 2193.144 kb on + strand, within DDA3937_RS09710 at 2193.144 kb on + strand, within DDA3937_RS09710 at 2193.161 kb on + strand, within DDA3937_RS09710 at 2193.162 kb on - strand, within DDA3937_RS09710 at 2193.167 kb on - strand at 2193.187 kb on + strand at 2193.209 kb on + strand at 2193.210 kb on - strand at 2193.224 kb on + strand at 2193.224 kb on + strand at 2193.225 kb on - strand at 2193.225 kb on - strand at 2193.286 kb on - strand at 2193.286 kb on - strand at 2193.462 kb on + strand, within DDA3937_RS09715
Per-strain Table
Position Strand Gene LocusTag Fraction M9+glycerol remove 2,190,882 + -1.6 2,190,883 - -0.2 2,190,898 - -0.5 2,190,898 - -0.3 2,190,898 - +0.4 2,190,905 + +0.2 2,190,906 - +0.6 2,190,906 - -0.7 2,190,927 - -0.1 2,190,939 + -1.5 2,190,939 + -1.8 2,190,944 - -1.0 2,190,944 - +1.7 2,190,944 - +2.2 2,191,011 - -2.8 2,191,011 - -2.1 2,191,065 + DDA3937_RS09700 0.12 -0.8 2,191,065 + DDA3937_RS09700 0.12 -1.0 2,191,066 - DDA3937_RS09700 0.12 -1.0 2,191,069 + DDA3937_RS09700 0.12 +1.2 2,191,083 + DDA3937_RS09700 0.14 -2.1 2,191,083 + DDA3937_RS09700 0.14 -2.5 2,191,083 + DDA3937_RS09700 0.14 -0.8 2,191,084 - DDA3937_RS09700 0.15 -0.2 2,191,084 - DDA3937_RS09700 0.15 -1.2 2,191,086 - DDA3937_RS09700 0.15 -0.4 2,191,086 - DDA3937_RS09700 0.15 -0.8 2,191,086 - DDA3937_RS09700 0.15 -0.4 2,191,170 + DDA3937_RS09700 0.28 -0.9 2,191,204 + DDA3937_RS09700 0.33 +1.2 2,191,209 + DDA3937_RS09700 0.34 +0.2 2,191,250 + DDA3937_RS09700 0.41 -0.6 2,191,251 - +0.2 2,191,254 + DDA3937_RS09700 0.41 -2.0 2,191,254 + DDA3937_RS09700 0.41 -1.6 2,191,262 + DDA3937_RS09700 0.43 -2.7 2,191,266 + DDA3937_RS09700 0.43 -0.4 2,191,266 + DDA3937_RS09700 0.43 -0.4 2,191,266 + DDA3937_RS09700 0.43 +0.6 2,191,267 - DDA3937_RS09700 0.43 +0.2 2,191,267 - DDA3937_RS09700 0.43 -1.6 2,191,267 - DDA3937_RS09700 0.43 -1.5 2,191,269 - DDA3937_RS09700 0.44 +0.6 2,191,278 + -1.4 2,191,278 + +1.9 2,191,279 - DDA3937_RS09700 0.45 +0.6 2,191,279 - DDA3937_RS09700 0.45 -1.1 2,191,279 - DDA3937_RS09700 0.45 -0.2 2,191,279 - DDA3937_RS09700 0.45 +1.5 2,191,279 - DDA3937_RS09700 0.45 +0.0 2,191,320 + DDA3937_RS09700 0.52 -1.5 2,191,320 + DDA3937_RS09700 0.52 -0.1 2,191,320 + DDA3937_RS09700 0.52 +0.3 2,191,321 - DDA3937_RS09700 0.52 -1.1 2,191,321 - DDA3937_RS09700 0.52 -1.4 2,191,321 - DDA3937_RS09700 0.52 -2.1 2,191,321 - +0.8 2,191,322 + DDA3937_RS09700 0.52 -0.6 2,191,322 + DDA3937_RS09700 0.52 -2.4 2,191,322 + DDA3937_RS09700 0.52 +0.2 2,191,322 + DDA3937_RS09700 0.52 -0.8 2,191,322 + DDA3937_RS09700 0.52 -0.8 2,191,323 - DDA3937_RS09700 0.52 -1.4 2,191,323 - DDA3937_RS09700 0.52 +0.3 2,191,323 - DDA3937_RS09700 0.52 +0.7 2,191,323 - DDA3937_RS09700 0.52 -2.8 2,191,346 + DDA3937_RS09700 0.56 -1.4 2,191,363 - DDA3937_RS09700 0.58 -1.2 2,191,376 + DDA3937_RS09700 0.61 -1.6 2,191,376 + DDA3937_RS09700 0.61 +2.9 2,191,376 + DDA3937_RS09700 0.61 -0.2 2,191,376 + DDA3937_RS09700 0.61 +1.6 2,191,377 - DDA3937_RS09700 0.61 -1.4 2,191,377 - DDA3937_RS09700 0.61 -0.7 2,191,377 - DDA3937_RS09700 0.61 -0.4 2,191,377 - -0.4 2,191,377 - DDA3937_RS09700 0.61 -1.9 2,191,377 - DDA3937_RS09700 0.61 -1.1 2,191,377 - DDA3937_RS09700 0.61 -0.2 2,191,377 - DDA3937_RS09700 0.61 -0.2 2,191,377 - DDA3937_RS09700 0.61 +0.3 2,191,377 - DDA3937_RS09700 0.61 -1.0 2,191,377 - DDA3937_RS09700 0.61 -1.1 2,191,377 - DDA3937_RS09700 0.61 +0.4 2,191,431 + DDA3937_RS09700 0.69 -1.4 2,191,431 + DDA3937_RS09700 0.69 +0.8 2,191,431 + -1.1 2,191,432 - DDA3937_RS09700 0.69 -1.1 2,191,463 - DDA3937_RS09700 0.74 -2.1 2,191,478 + DDA3937_RS09700 0.77 +0.3 2,191,478 + DDA3937_RS09700 0.77 +0.8 2,191,479 - DDA3937_RS09700 0.77 +3.8 2,191,479 - DDA3937_RS09700 0.77 +0.7 2,191,479 - DDA3937_RS09700 0.77 -1.6 2,191,497 + DDA3937_RS09700 0.80 -0.8 2,191,501 - DDA3937_RS09700 0.80 -0.5 2,191,589 - +0.6 2,191,604 + -0.6 2,191,604 + -0.5 2,191,604 + -0.8 2,191,627 - +0.5 2,191,656 + +0.5 2,191,738 - -1.7 2,191,738 - -1.6 2,191,944 + DDA3937_RS09705 0.11 -0.4 2,191,945 - DDA3937_RS09705 0.11 -1.0 2,191,945 - DDA3937_RS09705 0.11 -2.4 2,191,957 + DDA3937_RS09705 0.13 -1.7 2,191,957 + DDA3937_RS09705 0.13 -2.0 2,191,957 + DDA3937_RS09705 0.13 -2.4 2,192,075 - DDA3937_RS09705 0.33 +0.2 2,192,090 - DDA3937_RS09705 0.35 -2.5 2,192,115 - DDA3937_RS09705 0.39 -2.6 2,192,300 + DDA3937_RS09705 0.69 -2.9 2,192,300 + DDA3937_RS09705 0.69 +1.8 2,192,318 + DDA3937_RS09705 0.72 -3.0 2,192,319 - DDA3937_RS09705 0.72 -2.1 2,192,319 - DDA3937_RS09705 0.72 -3.6 2,192,321 + DDA3937_RS09705 0.73 -0.8 2,192,321 + DDA3937_RS09705 0.73 +0.6 2,192,330 - DDA3937_RS09705 0.74 -1.6 2,192,330 - DDA3937_RS09705 0.74 -1.6 2,192,383 + DDA3937_RS09705 0.83 -2.0 2,192,384 - DDA3937_RS09705 0.83 -2.0 2,192,697 + DDA3937_RS09710 0.28 -1.6 2,192,698 - DDA3937_RS09710 0.28 -2.0 2,192,797 + DDA3937_RS09710 0.40 -1.3 2,192,827 - DDA3937_RS09710 0.44 -0.1 2,192,827 - DDA3937_RS09710 0.44 -1.4 2,192,827 - DDA3937_RS09710 0.44 -2.7 2,192,834 - DDA3937_RS09710 0.45 -1.8 2,192,869 + DDA3937_RS09710 0.50 -2.5 2,192,894 + DDA3937_RS09710 0.53 -2.3 2,192,894 + +0.2 2,192,895 - DDA3937_RS09710 0.53 -1.1 2,193,112 - DDA3937_RS09710 0.81 -3.8 2,193,112 - DDA3937_RS09710 0.81 -1.5 2,193,144 + DDA3937_RS09710 0.85 +0.2 2,193,144 + DDA3937_RS09710 0.85 -2.1 2,193,161 + DDA3937_RS09710 0.87 -1.8 2,193,162 - DDA3937_RS09710 0.87 -0.6 2,193,167 - +0.2 2,193,187 + +1.6 2,193,209 + -3.3 2,193,210 - +0.0 2,193,224 + +0.6 2,193,224 + -1.0 2,193,225 - +0.2 2,193,225 - -0.6 2,193,286 - -0.6 2,193,286 - -1.6 2,193,462 + DDA3937_RS09715 0.30 -0.4
Or see this region's nucleotide sequence