Experiment: M9+glycerol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DDA3937_RS02625 and DDA3937_RS21820 are separated by 57 nucleotides DDA3937_RS21820 and DDA3937_RS02630 are separated by 282 nucleotides DDA3937_RS02630 and DDA3937_RS21825 are separated by 287 nucleotides DDA3937_RS21825 and DDA3937_RS22990 are separated by 11 nucleotides
DDA3937_RS02625: DDA3937_RS02625 - DUF1778 domain-containing protein, at 581,656 to 581,949
_RS02625
DDA3937_RS21820: DDA3937_RS21820 - GNAT family N-acetyltransferase, at 582,007 to 582,430
_RS21820
DDA3937_RS02630: DDA3937_RS02630 - TIGR04141 family sporadically distributed protein, at 582,713 to 584,452
_RS02630
DDA3937_RS21825: DDA3937_RS21825 - type II toxin-antitoxin system toxin CcdB, at 584,740 to 585,034
_RS21825
DDA3937_RS22990: DDA3937_RS22990 - type II toxin-antitoxin system CcdA family antitoxin, at 585,046 to 585,147
_RS22990
Position (kb)
582
583
584
585 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 581.984 kb on + strand at 581.984 kb on + strand at 581.984 kb on + strand at 581.984 kb on + strand at 581.984 kb on + strand at 581.984 kb on + strand at 581.984 kb on + strand at 581.985 kb on - strand at 581.985 kb on - strand at 581.985 kb on - strand at 581.987 kb on + strand at 581.987 kb on + strand at 581.987 kb on + strand at 581.987 kb on + strand at 581.988 kb on - strand at 581.988 kb on - strand at 581.988 kb on - strand at 581.988 kb on - strand at 581.988 kb on - strand at 582.015 kb on + strand at 582.016 kb on - strand at 582.109 kb on + strand, within DDA3937_RS21820 at 582.109 kb on + strand, within DDA3937_RS21820 at 582.109 kb on + strand, within DDA3937_RS21820 at 582.110 kb on - strand, within DDA3937_RS21820 at 582.112 kb on + strand, within DDA3937_RS21820 at 582.112 kb on + strand, within DDA3937_RS21820 at 582.112 kb on + strand at 582.134 kb on + strand, within DDA3937_RS21820 at 582.135 kb on - strand, within DDA3937_RS21820 at 582.172 kb on - strand, within DDA3937_RS21820 at 582.172 kb on - strand, within DDA3937_RS21820 at 582.172 kb on - strand, within DDA3937_RS21820 at 582.172 kb on - strand, within DDA3937_RS21820 at 582.272 kb on + strand, within DDA3937_RS21820 at 582.272 kb on + strand, within DDA3937_RS21820 at 582.272 kb on + strand, within DDA3937_RS21820 at 582.273 kb on - strand, within DDA3937_RS21820 at 582.293 kb on - strand, within DDA3937_RS21820 at 582.341 kb on + strand, within DDA3937_RS21820 at 582.341 kb on + strand, within DDA3937_RS21820 at 582.420 kb on + strand at 582.440 kb on + strand at 582.440 kb on + strand at 582.440 kb on + strand at 582.440 kb on + strand at 582.441 kb on - strand at 582.441 kb on - strand at 582.441 kb on - strand at 582.441 kb on - strand at 582.441 kb on - strand at 582.441 kb on - strand at 582.484 kb on + strand at 582.488 kb on + strand at 582.489 kb on - strand at 582.489 kb on - strand at 582.494 kb on - strand at 582.494 kb on - strand at 582.494 kb on - strand at 582.522 kb on + strand at 582.523 kb on - strand at 582.523 kb on - strand at 582.523 kb on - strand at 582.526 kb on + strand at 582.526 kb on + strand at 582.527 kb on - strand at 582.539 kb on - strand at 582.561 kb on - strand at 582.646 kb on - strand at 582.707 kb on + strand at 582.707 kb on + strand at 582.769 kb on - strand at 582.812 kb on - strand at 582.858 kb on - strand at 582.859 kb on + strand at 582.944 kb on - strand, within DDA3937_RS02630 at 583.127 kb on - strand, within DDA3937_RS02630 at 583.294 kb on + strand, within DDA3937_RS02630 at 583.347 kb on + strand, within DDA3937_RS02630 at 583.367 kb on - strand, within DDA3937_RS02630 at 583.402 kb on + strand, within DDA3937_RS02630 at 583.733 kb on + strand, within DDA3937_RS02630 at 583.808 kb on - strand, within DDA3937_RS02630 at 584.180 kb on - strand, within DDA3937_RS02630 at 584.333 kb on + strand at 584.372 kb on - strand at 584.439 kb on + strand at 584.461 kb on + strand at 584.462 kb on - strand at 584.463 kb on + strand at 584.466 kb on - strand at 584.468 kb on - strand at 584.557 kb on - strand at 584.593 kb on + strand at 584.617 kb on - strand at 584.764 kb on - strand at 584.767 kb on - strand at 585.090 kb on + strand, within DDA3937_RS22990 at 585.150 kb on - strand at 585.230 kb on - strand at 585.249 kb on - strand at 585.264 kb on + strand at 585.265 kb on - strand at 585.299 kb on + strand at 585.432 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9+glycerol remove 581,984 + +0.2 581,984 + -0.1 581,984 + -0.8 581,984 + +1.6 581,984 + -0.3 581,984 + +0.3 581,984 + -1.5 581,985 - -1.4 581,985 - -0.5 581,985 - -0.3 581,987 + -0.1 581,987 + -1.4 581,987 + +1.0 581,987 + -0.3 581,988 - -0.5 581,988 - +0.8 581,988 - -0.9 581,988 - +0.2 581,988 - -0.6 582,015 + -2.0 582,016 - +1.9 582,109 + DDA3937_RS21820 0.24 +0.2 582,109 + DDA3937_RS21820 0.24 +0.5 582,109 + DDA3937_RS21820 0.24 +0.2 582,110 - DDA3937_RS21820 0.24 -1.0 582,112 + DDA3937_RS21820 0.25 +0.9 582,112 + DDA3937_RS21820 0.25 -0.8 582,112 + +1.2 582,134 + DDA3937_RS21820 0.30 -1.5 582,135 - DDA3937_RS21820 0.30 -1.4 582,172 - DDA3937_RS21820 0.39 -2.7 582,172 - DDA3937_RS21820 0.39 -1.7 582,172 - DDA3937_RS21820 0.39 -2.1 582,172 - DDA3937_RS21820 0.39 -0.6 582,272 + DDA3937_RS21820 0.62 -0.6 582,272 + DDA3937_RS21820 0.62 -1.0 582,272 + DDA3937_RS21820 0.62 -0.8 582,273 - DDA3937_RS21820 0.63 -1.9 582,293 - DDA3937_RS21820 0.67 -1.1 582,341 + DDA3937_RS21820 0.79 -0.5 582,341 + DDA3937_RS21820 0.79 -0.4 582,420 + -1.0 582,440 + +0.1 582,440 + -0.8 582,440 + -2.3 582,440 + -0.8 582,441 - -0.3 582,441 - -0.5 582,441 - +1.3 582,441 - -0.6 582,441 - -1.0 582,441 - -1.2 582,484 + +1.4 582,488 + -1.2 582,489 - +0.5 582,489 - -0.6 582,494 - -0.3 582,494 - +0.5 582,494 - +0.4 582,522 + -1.4 582,523 - +1.8 582,523 - -0.8 582,523 - +0.2 582,526 + +0.8 582,526 + -0.6 582,527 - +0.6 582,539 - -1.8 582,561 - -0.1 582,646 - -0.7 582,707 + -0.4 582,707 + -1.5 582,769 - +1.8 582,812 - +0.2 582,858 - -0.8 582,859 + -1.4 582,944 - DDA3937_RS02630 0.13 -0.8 583,127 - DDA3937_RS02630 0.24 -1.2 583,294 + DDA3937_RS02630 0.33 -1.8 583,347 + DDA3937_RS02630 0.36 -0.5 583,367 - DDA3937_RS02630 0.38 -0.0 583,402 + DDA3937_RS02630 0.40 -0.7 583,733 + DDA3937_RS02630 0.59 -1.5 583,808 - DDA3937_RS02630 0.63 -1.1 584,180 - DDA3937_RS02630 0.84 -0.2 584,333 + -2.3 584,372 - -0.4 584,439 + -1.3 584,461 + -0.1 584,462 - -0.9 584,463 + -0.4 584,466 - -0.0 584,468 - +0.2 584,557 - -0.4 584,593 + -1.0 584,617 - -1.3 584,764 - -0.5 584,767 - +0.7 585,090 + DDA3937_RS22990 0.43 +0.2 585,150 - -1.7 585,230 - +1.1 585,249 - +0.8 585,264 + -0.7 585,265 - +2.9 585,299 + -2.3 585,432 + +0.4
Or see this region's nucleotide sequence