Experiment: M9+glycerol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DDA3937_RS01155 and DDA3937_RS01160 are separated by 1 nucleotides DDA3937_RS01160 and DDA3937_RS01165 overlap by 4 nucleotides DDA3937_RS01165 and DDA3937_RS01170 are separated by 1 nucleotides DDA3937_RS01170 and DDA3937_RS01175 overlap by 1 nucleotides
DDA3937_RS01155: DDA3937_RS01155 - thiazole synthase, at 265,273 to 266,052
_RS01155
DDA3937_RS01160: DDA3937_RS01160 - sulfur carrier protein ThiS, at 266,054 to 266,254
_RS01160
DDA3937_RS01165: DDA3937_RS01165 - HesA/MoeB/ThiF family protein, at 266,251 to 267,000
_RS01165
DDA3937_RS01170: DDA3937_RS01170 - thiamine phosphate synthase, at 267,002 to 267,637
_RS01170
DDA3937_RS01175: DDA3937_RS01175 - phosphomethylpyrimidine synthase ThiC, at 267,637 to 269,508
_RS01175
Position (kb)
266
267
268 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 265.258 kb on + strand at 265.274 kb on + strand at 265.274 kb on + strand at 265.274 kb on + strand at 265.513 kb on + strand, within DDA3937_RS01155 at 265.513 kb on + strand, within DDA3937_RS01155 at 265.514 kb on - strand, within DDA3937_RS01155 at 265.514 kb on - strand, within DDA3937_RS01155 at 265.514 kb on - strand, within DDA3937_RS01155 at 265.514 kb on - strand, within DDA3937_RS01155 at 265.514 kb on - strand, within DDA3937_RS01155 at 265.742 kb on + strand, within DDA3937_RS01155 at 265.742 kb on + strand, within DDA3937_RS01155 at 265.742 kb on + strand, within DDA3937_RS01155 at 265.742 kb on + strand, within DDA3937_RS01155 at 265.743 kb on - strand, within DDA3937_RS01155 at 265.743 kb on - strand, within DDA3937_RS01155 at 265.743 kb on - strand, within DDA3937_RS01155 at 265.743 kb on - strand, within DDA3937_RS01155 at 265.743 kb on - strand, within DDA3937_RS01155 at 265.864 kb on + strand, within DDA3937_RS01155 at 265.865 kb on - strand, within DDA3937_RS01155 at 266.014 kb on + strand at 266.025 kb on + strand at 266.026 kb on - strand at 266.026 kb on - strand at 266.026 kb on - strand at 266.363 kb on - strand, within DDA3937_RS01165 at 266.489 kb on + strand, within DDA3937_RS01165 at 266.489 kb on + strand, within DDA3937_RS01165 at 266.490 kb on - strand, within DDA3937_RS01165 at 266.490 kb on - strand, within DDA3937_RS01165 at 266.490 kb on - strand, within DDA3937_RS01165 at 266.505 kb on - strand, within DDA3937_RS01165 at 266.773 kb on - strand, within DDA3937_RS01165 at 266.861 kb on + strand, within DDA3937_RS01165 at 266.861 kb on + strand, within DDA3937_RS01165 at 266.862 kb on - strand, within DDA3937_RS01165 at 266.862 kb on - strand, within DDA3937_RS01165 at 266.920 kb on + strand, within DDA3937_RS01165 at 266.920 kb on + strand, within DDA3937_RS01165 at 266.921 kb on - strand, within DDA3937_RS01165 at 266.973 kb on - strand at 266.979 kb on + strand at 266.980 kb on - strand at 267.250 kb on + strand, within DDA3937_RS01170 at 267.250 kb on + strand, within DDA3937_RS01170 at 267.250 kb on + strand, within DDA3937_RS01170 at 267.250 kb on + strand, within DDA3937_RS01170 at 267.267 kb on + strand, within DDA3937_RS01170 at 267.267 kb on + strand, within DDA3937_RS01170 at 267.267 kb on + strand, within DDA3937_RS01170 at 267.267 kb on + strand, within DDA3937_RS01170 at 267.268 kb on - strand, within DDA3937_RS01170 at 267.268 kb on - strand, within DDA3937_RS01170 at 267.386 kb on + strand, within DDA3937_RS01170 at 267.386 kb on + strand, within DDA3937_RS01170 at 267.387 kb on - strand, within DDA3937_RS01170 at 267.387 kb on - strand, within DDA3937_RS01170 at 267.387 kb on - strand, within DDA3937_RS01170 at 267.393 kb on - strand, within DDA3937_RS01170 at 267.400 kb on - strand, within DDA3937_RS01170 at 267.400 kb on - strand, within DDA3937_RS01170 at 267.430 kb on - strand, within DDA3937_RS01170 at 267.674 kb on + strand at 267.765 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9+glycerol remove 265,258 + -0.2 265,274 + -1.9 265,274 + -1.3 265,274 + +0.9 265,513 + DDA3937_RS01155 0.31 -2.1 265,513 + DDA3937_RS01155 0.31 -1.4 265,514 - DDA3937_RS01155 0.31 -0.3 265,514 - DDA3937_RS01155 0.31 -2.6 265,514 - DDA3937_RS01155 0.31 -2.1 265,514 - DDA3937_RS01155 0.31 -0.6 265,514 - DDA3937_RS01155 0.31 -0.1 265,742 + DDA3937_RS01155 0.60 -1.9 265,742 + DDA3937_RS01155 0.60 -2.4 265,742 + DDA3937_RS01155 0.60 -1.9 265,742 + DDA3937_RS01155 0.60 +0.1 265,743 - DDA3937_RS01155 0.60 -0.4 265,743 - DDA3937_RS01155 0.60 -1.1 265,743 - DDA3937_RS01155 0.60 -2.1 265,743 - DDA3937_RS01155 0.60 -0.7 265,743 - DDA3937_RS01155 0.60 -0.1 265,864 + DDA3937_RS01155 0.76 -2.8 265,865 - DDA3937_RS01155 0.76 +0.1 266,014 + -0.3 266,025 + -1.5 266,026 - +0.3 266,026 - +0.0 266,026 - -2.1 266,363 - DDA3937_RS01165 0.15 -0.4 266,489 + DDA3937_RS01165 0.32 -0.6 266,489 + DDA3937_RS01165 0.32 -1.4 266,490 - DDA3937_RS01165 0.32 -0.7 266,490 - DDA3937_RS01165 0.32 -2.7 266,490 - DDA3937_RS01165 0.32 -3.2 266,505 - DDA3937_RS01165 0.34 -1.8 266,773 - DDA3937_RS01165 0.70 -0.9 266,861 + DDA3937_RS01165 0.81 -1.2 266,861 + DDA3937_RS01165 0.81 +0.8 266,862 - DDA3937_RS01165 0.81 -1.5 266,862 - DDA3937_RS01165 0.81 -1.6 266,920 + DDA3937_RS01165 0.89 -1.4 266,920 + DDA3937_RS01165 0.89 +0.9 266,921 - DDA3937_RS01165 0.89 +0.2 266,973 - -1.6 266,979 + -0.7 266,980 - -2.0 267,250 + DDA3937_RS01170 0.39 +0.1 267,250 + DDA3937_RS01170 0.39 -2.4 267,250 + DDA3937_RS01170 0.39 -3.1 267,250 + DDA3937_RS01170 0.39 +0.1 267,267 + DDA3937_RS01170 0.42 -2.4 267,267 + DDA3937_RS01170 0.42 -0.7 267,267 + DDA3937_RS01170 0.42 -4.3 267,267 + DDA3937_RS01170 0.42 -1.5 267,268 - DDA3937_RS01170 0.42 -1.7 267,268 - DDA3937_RS01170 0.42 -0.0 267,386 + DDA3937_RS01170 0.60 -0.4 267,386 + DDA3937_RS01170 0.60 +0.3 267,387 - DDA3937_RS01170 0.61 -1.1 267,387 - DDA3937_RS01170 0.61 +0.1 267,387 - DDA3937_RS01170 0.61 +0.9 267,393 - DDA3937_RS01170 0.61 -1.2 267,400 - DDA3937_RS01170 0.63 -0.8 267,400 - DDA3937_RS01170 0.63 +0.1 267,430 - DDA3937_RS01170 0.67 +0.2 267,674 + -2.4 267,765 - -1.0
Or see this region's nucleotide sequence