Experiment: M9+glycerol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DDA3937_RS02300 and DDA3937_RS02305 are separated by 560 nucleotides DDA3937_RS02305 and DDA3937_RS23400 are separated by 68 nucleotides DDA3937_RS23400 and DDA3937_RS02310 are separated by 23 nucleotides DDA3937_RS02310 and DDA3937_RS02315 are separated by 23 nucleotides
DDA3937_RS02300: DDA3937_RS02300 - MCP four helix bundle domain-containing protein, at 509,628 to 511,217
_RS02300
DDA3937_RS02305: DDA3937_RS02305 - L-rhamnose mutarotase, at 511,778 to 512,092
_RS02305
DDA3937_RS23400: DDA3937_RS23400 - hypothetical protein, at 512,161 to 512,337
_RS23400
DDA3937_RS02310: DDA3937_RS02310 - rhamnulose-1-phosphate aldolase, at 512,361 to 513,197
_RS02310
DDA3937_RS02315: DDA3937_RS02315 - L-rhamnose isomerase, at 513,221 to 514,477
_RS02315
Position (kb) 512
513 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 511.244 kb on - strand at 511.244 kb on - strand at 511.244 kb on - strand at 511.263 kb on + strand at 511.458 kb on + strand at 511.492 kb on - strand at 511.575 kb on + strand at 511.576 kb on - strand at 511.579 kb on + strand at 511.585 kb on + strand at 511.585 kb on + strand at 511.604 kb on + strand at 511.604 kb on + strand at 511.605 kb on - strand at 511.605 kb on - strand at 511.625 kb on + strand at 511.625 kb on + strand at 511.626 kb on - strand at 511.626 kb on - strand at 511.626 kb on - strand at 511.692 kb on - strand at 511.712 kb on + strand at 511.712 kb on + strand at 511.712 kb on + strand at 511.712 kb on + strand at 511.712 kb on + strand at 511.713 kb on - strand at 511.713 kb on - strand at 511.713 kb on - strand at 511.713 kb on - strand at 511.713 kb on - strand at 511.760 kb on - strand at 511.775 kb on + strand at 511.776 kb on - strand at 511.776 kb on - strand at 511.779 kb on + strand at 511.780 kb on - strand at 511.857 kb on + strand, within DDA3937_RS02305 at 511.857 kb on + strand, within DDA3937_RS02305 at 511.858 kb on - strand, within DDA3937_RS02305 at 511.929 kb on + strand, within DDA3937_RS02305 at 511.930 kb on - strand, within DDA3937_RS02305 at 511.930 kb on - strand, within DDA3937_RS02305 at 511.971 kb on + strand, within DDA3937_RS02305 at 511.971 kb on + strand, within DDA3937_RS02305 at 511.971 kb on + strand, within DDA3937_RS02305 at 511.972 kb on - strand, within DDA3937_RS02305 at 511.972 kb on - strand, within DDA3937_RS02305 at 511.972 kb on - strand, within DDA3937_RS02305 at 511.972 kb on - strand at 512.040 kb on + strand, within DDA3937_RS02305 at 512.041 kb on - strand, within DDA3937_RS02305 at 512.041 kb on - strand, within DDA3937_RS02305 at 512.119 kb on + strand at 512.119 kb on + strand at 512.119 kb on + strand at 512.119 kb on + strand at 512.120 kb on - strand at 512.120 kb on - strand at 512.120 kb on - strand at 512.149 kb on + strand at 512.149 kb on + strand at 512.149 kb on + strand at 512.149 kb on + strand at 512.149 kb on + strand at 512.150 kb on - strand at 512.275 kb on + strand, within DDA3937_RS23400 at 512.276 kb on - strand, within DDA3937_RS23400 at 512.276 kb on - strand, within DDA3937_RS23400 at 512.325 kb on - strand at 512.347 kb on + strand at 512.362 kb on + strand at 512.366 kb on + strand at 512.377 kb on + strand at 512.378 kb on - strand at 512.378 kb on - strand at 512.382 kb on + strand at 512.383 kb on - strand at 512.383 kb on - strand at 512.743 kb on + strand, within DDA3937_RS02310 at 512.743 kb on + strand, within DDA3937_RS02310 at 512.743 kb on + strand, within DDA3937_RS02310 at 512.743 kb on + strand, within DDA3937_RS02310 at 512.743 kb on + strand, within DDA3937_RS02310 at 512.744 kb on - strand, within DDA3937_RS02310 at 512.744 kb on - strand, within DDA3937_RS02310 at 512.744 kb on - strand, within DDA3937_RS02310 at 512.744 kb on - strand, within DDA3937_RS02310 at 512.744 kb on - strand, within DDA3937_RS02310 at 513.049 kb on + strand, within DDA3937_RS02310 at 513.049 kb on + strand, within DDA3937_RS02310 at 513.168 kb on + strand at 513.168 kb on + strand at 513.169 kb on - strand at 513.186 kb on + strand at 513.187 kb on - strand at 513.222 kb on + strand at 513.223 kb on - strand at 513.249 kb on + strand at 513.306 kb on + strand at 513.306 kb on + strand at 513.306 kb on + strand at 513.306 kb on + strand at 513.306 kb on + strand at 513.306 kb on + strand at 513.307 kb on - strand at 513.307 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9+glycerol remove 511,244 - +1.4 511,244 - -1.0 511,244 - +0.6 511,263 + -0.6 511,458 + -2.5 511,492 - +0.6 511,575 + -1.9 511,576 - -2.8 511,579 + -2.5 511,585 + +0.6 511,585 + +0.2 511,604 + -1.9 511,604 + -1.4 511,605 - -0.1 511,605 - +0.8 511,625 + -1.7 511,625 + +0.9 511,626 - +0.7 511,626 - +0.8 511,626 - -0.2 511,692 - -0.3 511,712 + -1.0 511,712 + -0.4 511,712 + -2.1 511,712 + +1.8 511,712 + -0.1 511,713 - -0.7 511,713 - -0.7 511,713 - -1.0 511,713 - -0.4 511,713 - -0.0 511,760 - -0.0 511,775 + -1.4 511,776 - -0.1 511,776 - -0.1 511,779 + -0.2 511,780 - -0.7 511,857 + DDA3937_RS02305 0.25 -2.1 511,857 + DDA3937_RS02305 0.25 -1.9 511,858 - DDA3937_RS02305 0.25 +0.5 511,929 + DDA3937_RS02305 0.48 -0.9 511,930 - DDA3937_RS02305 0.48 +2.4 511,930 - DDA3937_RS02305 0.48 -1.2 511,971 + DDA3937_RS02305 0.61 +0.4 511,971 + DDA3937_RS02305 0.61 -0.5 511,971 + DDA3937_RS02305 0.61 -0.3 511,972 - DDA3937_RS02305 0.62 -0.3 511,972 - DDA3937_RS02305 0.62 -1.3 511,972 - DDA3937_RS02305 0.62 +0.5 511,972 - +0.3 512,040 + DDA3937_RS02305 0.83 +0.3 512,041 - DDA3937_RS02305 0.83 -1.0 512,041 - DDA3937_RS02305 0.83 +0.9 512,119 + -0.1 512,119 + -0.6 512,119 + +1.2 512,119 + -0.0 512,120 - -0.8 512,120 - -2.4 512,120 - -0.0 512,149 + -2.1 512,149 + -2.7 512,149 + -1.6 512,149 + -0.4 512,149 + -0.3 512,150 - +0.8 512,275 + DDA3937_RS23400 0.64 -0.6 512,276 - DDA3937_RS23400 0.65 -1.2 512,276 - DDA3937_RS23400 0.65 -1.5 512,325 - -1.4 512,347 + +0.9 512,362 + -0.2 512,366 + +1.6 512,377 + -0.8 512,378 - -0.3 512,378 - -0.1 512,382 + +0.2 512,383 - -1.5 512,383 - +2.8 512,743 + DDA3937_RS02310 0.46 -0.2 512,743 + DDA3937_RS02310 0.46 -0.2 512,743 + DDA3937_RS02310 0.46 +0.7 512,743 + DDA3937_RS02310 0.46 -0.3 512,743 + DDA3937_RS02310 0.46 -0.2 512,744 - DDA3937_RS02310 0.46 -0.1 512,744 - DDA3937_RS02310 0.46 -0.0 512,744 - DDA3937_RS02310 0.46 -1.1 512,744 - DDA3937_RS02310 0.46 -1.1 512,744 - DDA3937_RS02310 0.46 -0.1 513,049 + DDA3937_RS02310 0.82 +1.1 513,049 + DDA3937_RS02310 0.82 +0.1 513,168 + -0.9 513,168 + +0.1 513,169 - -0.8 513,186 + -0.8 513,187 - -0.8 513,222 + +1.6 513,223 - -0.8 513,249 + -1.7 513,306 + +2.6 513,306 + -0.1 513,306 + -0.1 513,306 + +0.8 513,306 + +0.8 513,306 + -0.4 513,307 - -2.4 513,307 - -0.2
Or see this region's nucleotide sequence