Experiment: M9+glycerol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DDA3937_RS13720 and DDA3937_RS13725 are separated by 75 nucleotides DDA3937_RS13725 and DDA3937_RS13730 are separated by 384 nucleotides DDA3937_RS13730 and DDA3937_RS13735 are separated by 161 nucleotides DDA3937_RS13735 and DDA3937_RS13740 are separated by 154 nucleotides
DDA3937_RS13720: DDA3937_RS13720 - D-serine/D-alanine/glycine transporter, at 3,124,304 to 3,125,707
_RS13720
DDA3937_RS13725: DDA3937_RS13725 - LysR family transcriptional regulator, at 3,125,783 to 3,126,049
_RS13725
DDA3937_RS13730: DDA3937_RS13730 - tRNA-Asn, at 3,126,434 to 3,126,509
_RS13730
DDA3937_RS13735: DDA3937_RS13735 - integrase arm-type DNA-binding domain-containing protein, at 3,126,671 to 3,127,954
_RS13735
DDA3937_RS13740: DDA3937_RS13740 - DUF736 domain-containing protein, at 3,128,109 to 3,128,420
_RS13740
Position (kb)
3126
3127
3128 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3125.714 kb on - strand at 3125.733 kb on + strand at 3125.734 kb on - strand at 3125.744 kb on - strand at 3125.744 kb on - strand at 3125.744 kb on - strand at 3125.744 kb on - strand at 3125.757 kb on + strand at 3125.757 kb on + strand at 3125.758 kb on - strand at 3125.758 kb on - strand at 3125.758 kb on - strand at 3125.775 kb on + strand at 3125.795 kb on + strand at 3126.028 kb on - strand at 3126.064 kb on + strand at 3126.068 kb on + strand at 3126.068 kb on + strand at 3126.068 kb on + strand at 3126.069 kb on - strand at 3126.069 kb on - strand at 3126.074 kb on + strand at 3126.075 kb on - strand at 3126.078 kb on + strand at 3126.078 kb on + strand at 3126.079 kb on - strand at 3126.079 kb on - strand at 3126.082 kb on - strand at 3126.082 kb on - strand at 3126.146 kb on + strand at 3126.156 kb on + strand at 3126.189 kb on + strand at 3126.189 kb on + strand at 3126.190 kb on - strand at 3126.190 kb on - strand at 3126.190 kb on - strand at 3126.192 kb on + strand at 3126.192 kb on + strand at 3126.264 kb on - strand at 3126.264 kb on - strand at 3126.331 kb on - strand at 3126.331 kb on - strand at 3126.348 kb on + strand at 3126.411 kb on + strand at 3126.427 kb on + strand at 3126.427 kb on + strand at 3126.476 kb on + strand, within DDA3937_RS13730 at 3126.476 kb on + strand, within DDA3937_RS13730 at 3126.542 kb on + strand at 3126.565 kb on + strand at 3126.575 kb on + strand at 3126.679 kb on + strand at 3126.702 kb on + strand at 3126.756 kb on + strand at 3126.756 kb on + strand at 3126.756 kb on + strand at 3126.757 kb on - strand at 3126.757 kb on - strand at 3126.757 kb on - strand at 3126.786 kb on - strand at 3126.795 kb on - strand at 3126.805 kb on + strand, within DDA3937_RS13735 at 3126.806 kb on - strand, within DDA3937_RS13735 at 3126.828 kb on + strand, within DDA3937_RS13735 at 3126.828 kb on + strand at 3126.829 kb on - strand, within DDA3937_RS13735 at 3126.829 kb on - strand, within DDA3937_RS13735 at 3126.832 kb on + strand, within DDA3937_RS13735 at 3126.833 kb on - strand, within DDA3937_RS13735 at 3126.836 kb on + strand, within DDA3937_RS13735 at 3126.837 kb on - strand, within DDA3937_RS13735 at 3126.837 kb on - strand, within DDA3937_RS13735 at 3126.846 kb on + strand, within DDA3937_RS13735 at 3126.886 kb on + strand, within DDA3937_RS13735 at 3126.904 kb on + strand, within DDA3937_RS13735 at 3126.904 kb on + strand, within DDA3937_RS13735 at 3127.049 kb on + strand, within DDA3937_RS13735 at 3127.212 kb on + strand, within DDA3937_RS13735 at 3127.213 kb on - strand, within DDA3937_RS13735 at 3127.213 kb on - strand, within DDA3937_RS13735 at 3127.246 kb on + strand, within DDA3937_RS13735 at 3127.247 kb on - strand, within DDA3937_RS13735 at 3127.271 kb on + strand, within DDA3937_RS13735 at 3127.297 kb on - strand, within DDA3937_RS13735 at 3127.435 kb on + strand, within DDA3937_RS13735 at 3127.447 kb on + strand, within DDA3937_RS13735 at 3127.520 kb on - strand, within DDA3937_RS13735 at 3127.520 kb on - strand, within DDA3937_RS13735 at 3127.520 kb on - strand, within DDA3937_RS13735 at 3127.550 kb on - strand, within DDA3937_RS13735 at 3127.563 kb on + strand, within DDA3937_RS13735 at 3127.563 kb on + strand, within DDA3937_RS13735 at 3127.564 kb on - strand, within DDA3937_RS13735 at 3127.564 kb on - strand, within DDA3937_RS13735 at 3127.564 kb on - strand, within DDA3937_RS13735 at 3127.593 kb on + strand, within DDA3937_RS13735 at 3127.594 kb on - strand, within DDA3937_RS13735 at 3127.616 kb on + strand, within DDA3937_RS13735 at 3127.617 kb on - strand, within DDA3937_RS13735 at 3127.619 kb on - strand, within DDA3937_RS13735 at 3127.619 kb on - strand, within DDA3937_RS13735 at 3127.653 kb on + strand, within DDA3937_RS13735 at 3127.653 kb on + strand, within DDA3937_RS13735 at 3127.653 kb on + strand, within DDA3937_RS13735 at 3127.654 kb on - strand, within DDA3937_RS13735 at 3127.805 kb on + strand, within DDA3937_RS13735 at 3127.805 kb on + strand, within DDA3937_RS13735 at 3127.805 kb on + strand, within DDA3937_RS13735 at 3127.805 kb on + strand, within DDA3937_RS13735 at 3127.805 kb on + strand, within DDA3937_RS13735 at 3127.805 kb on + strand, within DDA3937_RS13735 at 3127.806 kb on - strand, within DDA3937_RS13735 at 3127.806 kb on - strand at 3127.806 kb on - strand, within DDA3937_RS13735 at 3127.806 kb on - strand, within DDA3937_RS13735 at 3127.806 kb on - strand, within DDA3937_RS13735 at 3127.806 kb on - strand, within DDA3937_RS13735 at 3127.813 kb on + strand, within DDA3937_RS13735 at 3127.813 kb on + strand, within DDA3937_RS13735 at 3127.813 kb on + strand, within DDA3937_RS13735 at 3127.823 kb on + strand, within DDA3937_RS13735 at 3127.865 kb on + strand at 3127.889 kb on + strand at 3127.890 kb on - strand at 3127.901 kb on + strand at 3127.901 kb on + strand at 3127.907 kb on + strand at 3127.907 kb on + strand at 3127.908 kb on - strand at 3127.908 kb on - strand at 3127.935 kb on + strand at 3127.936 kb on - strand at 3127.962 kb on + strand at 3127.963 kb on - strand at 3127.963 kb on - strand at 3128.000 kb on + strand at 3128.000 kb on + strand at 3128.000 kb on + strand at 3128.000 kb on + strand at 3128.001 kb on - strand at 3128.001 kb on - strand at 3128.001 kb on - strand at 3128.001 kb on - strand at 3128.001 kb on - strand at 3128.001 kb on - strand at 3128.026 kb on + strand at 3128.062 kb on - strand at 3128.062 kb on - strand at 3128.062 kb on - strand at 3128.179 kb on + strand, within DDA3937_RS13740 at 3128.179 kb on + strand, within DDA3937_RS13740 at 3128.179 kb on + strand, within DDA3937_RS13740 at 3128.180 kb on - strand, within DDA3937_RS13740 at 3128.224 kb on + strand, within DDA3937_RS13740 at 3128.225 kb on - strand, within DDA3937_RS13740 at 3128.225 kb on - strand, within DDA3937_RS13740 at 3128.273 kb on - strand, within DDA3937_RS13740 at 3128.637 kb on - strand at 3128.798 kb on + strand at 3128.799 kb on - strand at 3128.832 kb on + strand at 3128.833 kb on - strand at 3128.833 kb on - strand at 3128.918 kb on + strand at 3128.918 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9+glycerol remove 3,125,714 - -0.0 3,125,733 + -0.6 3,125,734 - -0.7 3,125,744 - +0.4 3,125,744 - -0.3 3,125,744 - +2.1 3,125,744 - +0.3 3,125,757 + -2.3 3,125,757 + +0.5 3,125,758 - -2.3 3,125,758 - -1.3 3,125,758 - -1.8 3,125,775 + -0.3 3,125,795 + +0.6 3,126,028 - +1.5 3,126,064 + -0.3 3,126,068 + +3.3 3,126,068 + +1.0 3,126,068 + +0.3 3,126,069 - +0.2 3,126,069 - -1.5 3,126,074 + +0.7 3,126,075 - -0.5 3,126,078 + -0.0 3,126,078 + -1.6 3,126,079 - -3.0 3,126,079 - -0.3 3,126,082 - -3.4 3,126,082 - -0.6 3,126,146 + -1.0 3,126,156 + -0.3 3,126,189 + +0.5 3,126,189 + -0.2 3,126,190 - +2.4 3,126,190 - -1.6 3,126,190 - -1.2 3,126,192 + +0.8 3,126,192 + -1.8 3,126,264 - -1.3 3,126,264 - -0.5 3,126,331 - -0.0 3,126,331 - -1.8 3,126,348 + -0.0 3,126,411 + +0.3 3,126,427 + -0.2 3,126,427 + -1.4 3,126,476 + DDA3937_RS13730 0.55 +1.1 3,126,476 + DDA3937_RS13730 0.55 +0.9 3,126,542 + -1.3 3,126,565 + -1.5 3,126,575 + -0.5 3,126,679 + -1.6 3,126,702 + +0.8 3,126,756 + +1.4 3,126,756 + -0.6 3,126,756 + -1.0 3,126,757 - +0.4 3,126,757 - -1.3 3,126,757 - -1.1 3,126,786 - -0.5 3,126,795 - +0.8 3,126,805 + DDA3937_RS13735 0.10 -1.8 3,126,806 - DDA3937_RS13735 0.11 +0.5 3,126,828 + DDA3937_RS13735 0.12 -1.3 3,126,828 + +1.0 3,126,829 - DDA3937_RS13735 0.12 -2.0 3,126,829 - DDA3937_RS13735 0.12 -0.6 3,126,832 + DDA3937_RS13735 0.13 -0.4 3,126,833 - DDA3937_RS13735 0.13 +0.5 3,126,836 + DDA3937_RS13735 0.13 -1.8 3,126,837 - DDA3937_RS13735 0.13 +0.5 3,126,837 - DDA3937_RS13735 0.13 -1.1 3,126,846 + DDA3937_RS13735 0.14 +0.6 3,126,886 + DDA3937_RS13735 0.17 +0.3 3,126,904 + DDA3937_RS13735 0.18 +1.0 3,126,904 + DDA3937_RS13735 0.18 -0.6 3,127,049 + DDA3937_RS13735 0.29 -1.8 3,127,212 + DDA3937_RS13735 0.42 -2.3 3,127,213 - DDA3937_RS13735 0.42 -0.2 3,127,213 - DDA3937_RS13735 0.42 -1.4 3,127,246 + DDA3937_RS13735 0.45 -2.0 3,127,247 - DDA3937_RS13735 0.45 -0.3 3,127,271 + DDA3937_RS13735 0.47 -0.2 3,127,297 - DDA3937_RS13735 0.49 -2.3 3,127,435 + DDA3937_RS13735 0.60 -1.8 3,127,447 + DDA3937_RS13735 0.60 -0.6 3,127,520 - DDA3937_RS13735 0.66 -2.5 3,127,520 - DDA3937_RS13735 0.66 -0.6 3,127,520 - DDA3937_RS13735 0.66 -0.6 3,127,550 - DDA3937_RS13735 0.68 -2.0 3,127,563 + DDA3937_RS13735 0.69 -0.1 3,127,563 + DDA3937_RS13735 0.69 +0.7 3,127,564 - DDA3937_RS13735 0.70 +0.4 3,127,564 - DDA3937_RS13735 0.70 -1.0 3,127,564 - DDA3937_RS13735 0.70 -0.3 3,127,593 + DDA3937_RS13735 0.72 -1.1 3,127,594 - DDA3937_RS13735 0.72 -0.7 3,127,616 + DDA3937_RS13735 0.74 -2.0 3,127,617 - DDA3937_RS13735 0.74 +0.6 3,127,619 - DDA3937_RS13735 0.74 -2.7 3,127,619 - DDA3937_RS13735 0.74 -1.2 3,127,653 + DDA3937_RS13735 0.76 -2.6 3,127,653 + DDA3937_RS13735 0.76 -1.1 3,127,653 + DDA3937_RS13735 0.76 -0.7 3,127,654 - DDA3937_RS13735 0.77 -0.1 3,127,805 + DDA3937_RS13735 0.88 +0.7 3,127,805 + DDA3937_RS13735 0.88 -1.6 3,127,805 + DDA3937_RS13735 0.88 -2.3 3,127,805 + DDA3937_RS13735 0.88 -1.7 3,127,805 + DDA3937_RS13735 0.88 -0.8 3,127,805 + DDA3937_RS13735 0.88 -0.5 3,127,806 - DDA3937_RS13735 0.88 -0.1 3,127,806 - +2.6 3,127,806 - DDA3937_RS13735 0.88 -1.3 3,127,806 - DDA3937_RS13735 0.88 +0.2 3,127,806 - DDA3937_RS13735 0.88 -0.4 3,127,806 - DDA3937_RS13735 0.88 -0.4 3,127,813 + DDA3937_RS13735 0.89 -2.3 3,127,813 + DDA3937_RS13735 0.89 -0.3 3,127,813 + DDA3937_RS13735 0.89 +0.0 3,127,823 + DDA3937_RS13735 0.90 -2.7 3,127,865 + -3.3 3,127,889 + -1.0 3,127,890 - +1.8 3,127,901 + -1.6 3,127,901 + -1.0 3,127,907 + -1.7 3,127,907 + -0.6 3,127,908 - -1.6 3,127,908 - +2.3 3,127,935 + -1.1 3,127,936 - -0.1 3,127,962 + -0.6 3,127,963 - -0.0 3,127,963 - -1.0 3,128,000 + +0.2 3,128,000 + -0.9 3,128,000 + -0.7 3,128,000 + +1.1 3,128,001 - +0.1 3,128,001 - -1.3 3,128,001 - -1.6 3,128,001 - -0.0 3,128,001 - -1.7 3,128,001 - -1.3 3,128,026 + +0.8 3,128,062 - -1.6 3,128,062 - -0.6 3,128,062 - -2.7 3,128,179 + DDA3937_RS13740 0.22 -0.8 3,128,179 + DDA3937_RS13740 0.22 -2.2 3,128,179 + DDA3937_RS13740 0.22 +0.2 3,128,180 - DDA3937_RS13740 0.23 -0.1 3,128,224 + DDA3937_RS13740 0.37 -1.0 3,128,225 - DDA3937_RS13740 0.37 +0.5 3,128,225 - DDA3937_RS13740 0.37 +0.2 3,128,273 - DDA3937_RS13740 0.53 -1.2 3,128,637 - -0.3 3,128,798 + -1.6 3,128,799 - -0.2 3,128,832 + +0.6 3,128,833 - -0.8 3,128,833 - -1.6 3,128,918 + -2.0 3,128,918 + -0.1
Or see this region's nucleotide sequence