Experiment: M9+glycerol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DDA3937_RS19135 and DDA3937_RS19140 are separated by 50 nucleotides DDA3937_RS19140 and DDA3937_RS19145 are separated by 104 nucleotides DDA3937_RS19145 and DDA3937_RS22500 are separated by 5 nucleotides DDA3937_RS22500 and DDA3937_RS19150 are separated by 60 nucleotides
DDA3937_RS19135: DDA3937_RS19135 - 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB, at 4,374,131 to 4,375,426
_RS19135
DDA3937_RS19140: DDA3937_RS19140 - Trk system potassium transporter TrkA, at 4,375,477 to 4,376,853
_RS19140
DDA3937_RS19145: DDA3937_RS19145 - large-conductance mechanosensitive channel protein MscL, at 4,376,958 to 4,377,383
_RS19145
DDA3937_RS22500: DDA3937_RS22500 - ribosome alternative rescue factor ArfA, at 4,377,389 to 4,377,612
_RS22500
DDA3937_RS19150: DDA3937_RS19150 - DUF1992 domain-containing protein, at 4,377,673 to 4,378,059
_RS19150
Position (kb)
4375
4376
4377 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 4374.492 kb on + strand, within DDA3937_RS19135 at 4374.599 kb on + strand, within DDA3937_RS19135 at 4374.650 kb on - strand, within DDA3937_RS19135 at 4374.650 kb on - strand, within DDA3937_RS19135 at 4374.650 kb on - strand, within DDA3937_RS19135 at 4374.650 kb on - strand, within DDA3937_RS19135 at 4374.679 kb on + strand, within DDA3937_RS19135 at 4374.720 kb on + strand, within DDA3937_RS19135 at 4374.721 kb on - strand, within DDA3937_RS19135 at 4374.819 kb on + strand at 4374.921 kb on + strand, within DDA3937_RS19135 at 4374.921 kb on + strand, within DDA3937_RS19135 at 4374.921 kb on + strand, within DDA3937_RS19135 at 4374.922 kb on - strand at 4374.922 kb on - strand, within DDA3937_RS19135 at 4374.922 kb on - strand, within DDA3937_RS19135 at 4374.922 kb on - strand, within DDA3937_RS19135 at 4375.084 kb on + strand, within DDA3937_RS19135 at 4375.208 kb on - strand, within DDA3937_RS19135 at 4375.208 kb on - strand, within DDA3937_RS19135 at 4375.208 kb on - strand, within DDA3937_RS19135 at 4375.244 kb on + strand, within DDA3937_RS19135 at 4375.249 kb on + strand, within DDA3937_RS19135 at 4375.250 kb on - strand, within DDA3937_RS19135 at 4375.258 kb on + strand, within DDA3937_RS19135 at 4375.259 kb on - strand, within DDA3937_RS19135 at 4375.259 kb on - strand, within DDA3937_RS19135 at 4375.260 kb on + strand, within DDA3937_RS19135 at 4375.260 kb on + strand, within DDA3937_RS19135 at 4375.260 kb on + strand, within DDA3937_RS19135 at 4375.261 kb on - strand, within DDA3937_RS19135 at 4375.261 kb on - strand, within DDA3937_RS19135 at 4375.261 kb on - strand, within DDA3937_RS19135 at 4375.261 kb on - strand, within DDA3937_RS19135 at 4375.397 kb on + strand at 4375.410 kb on + strand at 4375.424 kb on + strand at 4375.424 kb on + strand at 4375.424 kb on + strand at 4375.425 kb on - strand at 4375.425 kb on - strand at 4375.441 kb on + strand at 4375.442 kb on - strand at 4375.442 kb on - strand at 4375.455 kb on + strand at 4375.455 kb on + strand at 4375.456 kb on - strand at 4375.484 kb on + strand at 4375.485 kb on - strand at 4375.572 kb on + strand at 4375.573 kb on - strand at 4375.646 kb on + strand, within DDA3937_RS19140 at 4375.787 kb on - strand, within DDA3937_RS19140 at 4375.787 kb on - strand, within DDA3937_RS19140 at 4375.789 kb on - strand, within DDA3937_RS19140 at 4375.872 kb on + strand, within DDA3937_RS19140 at 4375.873 kb on - strand, within DDA3937_RS19140 at 4375.910 kb on + strand, within DDA3937_RS19140 at 4375.910 kb on + strand, within DDA3937_RS19140 at 4375.939 kb on - strand, within DDA3937_RS19140 at 4375.957 kb on + strand, within DDA3937_RS19140 at 4375.957 kb on + strand, within DDA3937_RS19140 at 4375.958 kb on - strand, within DDA3937_RS19140 at 4375.960 kb on + strand, within DDA3937_RS19140 at 4375.960 kb on + strand, within DDA3937_RS19140 at 4376.007 kb on + strand, within DDA3937_RS19140 at 4376.007 kb on + strand, within DDA3937_RS19140 at 4376.007 kb on + strand, within DDA3937_RS19140 at 4376.007 kb on + strand, within DDA3937_RS19140 at 4376.018 kb on + strand, within DDA3937_RS19140 at 4376.018 kb on + strand, within DDA3937_RS19140 at 4376.170 kb on + strand, within DDA3937_RS19140 at 4376.171 kb on - strand, within DDA3937_RS19140 at 4376.172 kb on + strand, within DDA3937_RS19140 at 4376.172 kb on + strand, within DDA3937_RS19140 at 4376.172 kb on + strand, within DDA3937_RS19140 at 4376.172 kb on + strand, within DDA3937_RS19140 at 4376.173 kb on - strand, within DDA3937_RS19140 at 4376.173 kb on - strand at 4376.173 kb on - strand, within DDA3937_RS19140 at 4376.173 kb on - strand, within DDA3937_RS19140 at 4376.240 kb on - strand, within DDA3937_RS19140 at 4376.241 kb on + strand at 4376.255 kb on + strand, within DDA3937_RS19140 at 4376.297 kb on + strand, within DDA3937_RS19140 at 4376.298 kb on - strand, within DDA3937_RS19140 at 4376.375 kb on + strand, within DDA3937_RS19140 at 4376.479 kb on + strand, within DDA3937_RS19140 at 4376.579 kb on + strand, within DDA3937_RS19140 at 4376.586 kb on - strand at 4376.586 kb on - strand, within DDA3937_RS19140 at 4376.660 kb on + strand, within DDA3937_RS19140 at 4376.760 kb on - strand at 4376.781 kb on + strand at 4376.803 kb on + strand at 4376.804 kb on - strand at 4376.852 kb on - strand at 4376.869 kb on + strand at 4376.870 kb on - strand at 4376.870 kb on - strand at 4376.874 kb on - strand at 4376.874 kb on - strand at 4376.925 kb on - strand at 4376.955 kb on - strand at 4377.077 kb on + strand, within DDA3937_RS19145 at 4377.078 kb on - strand, within DDA3937_RS19145 at 4377.078 kb on - strand, within DDA3937_RS19145 at 4377.158 kb on + strand, within DDA3937_RS19145 at 4377.158 kb on + strand, within DDA3937_RS19145 at 4377.160 kb on + strand, within DDA3937_RS19145 at 4377.160 kb on + strand, within DDA3937_RS19145 at 4377.161 kb on - strand, within DDA3937_RS19145 at 4377.161 kb on - strand, within DDA3937_RS19145 at 4377.176 kb on + strand, within DDA3937_RS19145 at 4377.176 kb on + strand, within DDA3937_RS19145 at 4377.176 kb on + strand, within DDA3937_RS19145 at 4377.177 kb on - strand, within DDA3937_RS19145 at 4377.181 kb on - strand, within DDA3937_RS19145 at 4377.187 kb on + strand, within DDA3937_RS19145 at 4377.213 kb on - strand, within DDA3937_RS19145 at 4377.250 kb on + strand, within DDA3937_RS19145 at 4377.258 kb on - strand, within DDA3937_RS19145 at 4377.343 kb on + strand at 4377.344 kb on - strand at 4377.381 kb on + strand at 4377.382 kb on - strand at 4377.382 kb on - strand at 4377.382 kb on - strand at 4377.385 kb on + strand at 4377.385 kb on + strand at 4377.386 kb on - strand at 4377.386 kb on - strand at 4377.386 kb on - strand at 4377.390 kb on + strand at 4377.425 kb on - strand, within DDA3937_RS22500 at 4377.425 kb on - strand, within DDA3937_RS22500 at 4377.425 kb on - strand, within DDA3937_RS22500 at 4377.463 kb on - strand, within DDA3937_RS22500 at 4377.463 kb on - strand, within DDA3937_RS22500 at 4377.497 kb on + strand, within DDA3937_RS22500 at 4377.497 kb on + strand, within DDA3937_RS22500 at 4377.497 kb on + strand, within DDA3937_RS22500 at 4377.497 kb on + strand, within DDA3937_RS22500 at 4377.497 kb on + strand, within DDA3937_RS22500 at 4377.497 kb on + strand, within DDA3937_RS22500 at 4377.498 kb on - strand, within DDA3937_RS22500 at 4377.498 kb on - strand, within DDA3937_RS22500 at 4377.498 kb on - strand, within DDA3937_RS22500 at 4377.507 kb on + strand, within DDA3937_RS22500 at 4377.580 kb on + strand, within DDA3937_RS22500 at 4377.580 kb on + strand, within DDA3937_RS22500 at 4377.581 kb on - strand, within DDA3937_RS22500 at 4377.581 kb on - strand, within DDA3937_RS22500 at 4377.594 kb on + strand at 4377.612 kb on + strand at 4377.613 kb on - strand at 4377.628 kb on + strand at 4377.628 kb on + strand at 4377.629 kb on - strand at 4377.629 kb on - strand at 4377.629 kb on - strand at 4377.634 kb on + strand at 4377.635 kb on - strand at 4377.635 kb on - strand at 4377.635 kb on - strand at 4377.635 kb on - strand at 4377.684 kb on + strand at 4377.685 kb on - strand at 4377.693 kb on + strand at 4377.693 kb on + strand at 4377.693 kb on + strand at 4377.693 kb on + strand at 4377.693 kb on + strand at 4377.694 kb on - strand at 4377.715 kb on + strand, within DDA3937_RS19150 at 4377.715 kb on + strand, within DDA3937_RS19150 at 4377.715 kb on + strand, within DDA3937_RS19150 at 4377.716 kb on - strand, within DDA3937_RS19150 at 4377.716 kb on - strand, within DDA3937_RS19150 at 4377.716 kb on - strand, within DDA3937_RS19150 at 4377.728 kb on + strand, within DDA3937_RS19150 at 4377.729 kb on - strand, within DDA3937_RS19150 at 4377.729 kb on - strand, within DDA3937_RS19150 at 4377.729 kb on - strand, within DDA3937_RS19150 at 4377.763 kb on + strand, within DDA3937_RS19150 at 4377.776 kb on - strand, within DDA3937_RS19150 at 4377.812 kb on + strand, within DDA3937_RS19150 at 4377.812 kb on + strand, within DDA3937_RS19150 at 4377.845 kb on - strand, within DDA3937_RS19150 at 4377.845 kb on - strand, within DDA3937_RS19150
Per-strain Table
Position Strand Gene LocusTag Fraction M9+glycerol remove 4,374,492 + DDA3937_RS19135 0.28 +1.3 4,374,599 + DDA3937_RS19135 0.36 -1.2 4,374,650 - DDA3937_RS19135 0.40 -2.2 4,374,650 - DDA3937_RS19135 0.40 +1.3 4,374,650 - DDA3937_RS19135 0.40 +0.3 4,374,650 - DDA3937_RS19135 0.40 +0.3 4,374,679 + DDA3937_RS19135 0.42 -0.0 4,374,720 + DDA3937_RS19135 0.45 +0.7 4,374,721 - DDA3937_RS19135 0.46 +0.6 4,374,819 + +1.7 4,374,921 + DDA3937_RS19135 0.61 -1.3 4,374,921 + DDA3937_RS19135 0.61 +3.6 4,374,921 + DDA3937_RS19135 0.61 -1.7 4,374,922 - +1.3 4,374,922 - DDA3937_RS19135 0.61 +1.1 4,374,922 - DDA3937_RS19135 0.61 +0.6 4,374,922 - DDA3937_RS19135 0.61 +1.7 4,375,084 + DDA3937_RS19135 0.74 +0.8 4,375,208 - DDA3937_RS19135 0.83 +1.4 4,375,208 - DDA3937_RS19135 0.83 -0.0 4,375,208 - DDA3937_RS19135 0.83 +1.5 4,375,244 + DDA3937_RS19135 0.86 +0.3 4,375,249 + DDA3937_RS19135 0.86 -1.3 4,375,250 - DDA3937_RS19135 0.86 +0.3 4,375,258 + DDA3937_RS19135 0.87 +0.1 4,375,259 - DDA3937_RS19135 0.87 +0.3 4,375,259 - DDA3937_RS19135 0.87 +0.2 4,375,260 + DDA3937_RS19135 0.87 +0.4 4,375,260 + DDA3937_RS19135 0.87 +0.4 4,375,260 + DDA3937_RS19135 0.87 +1.3 4,375,261 - DDA3937_RS19135 0.87 -0.0 4,375,261 - DDA3937_RS19135 0.87 -0.2 4,375,261 - DDA3937_RS19135 0.87 +0.3 4,375,261 - DDA3937_RS19135 0.87 -1.9 4,375,397 + +1.0 4,375,410 + +0.3 4,375,424 + +0.3 4,375,424 + -0.6 4,375,424 + -0.3 4,375,425 - -0.5 4,375,425 - +0.6 4,375,441 + +0.6 4,375,442 - +0.3 4,375,442 - -0.7 4,375,455 + -3.0 4,375,455 + +0.3 4,375,456 - +1.0 4,375,484 + +0.3 4,375,485 - -1.4 4,375,572 + -0.3 4,375,573 - +0.3 4,375,646 + DDA3937_RS19140 0.12 -1.8 4,375,787 - DDA3937_RS19140 0.23 -0.5 4,375,787 - DDA3937_RS19140 0.23 -0.1 4,375,789 - DDA3937_RS19140 0.23 -0.5 4,375,872 + DDA3937_RS19140 0.29 -3.1 4,375,873 - DDA3937_RS19140 0.29 -2.6 4,375,910 + DDA3937_RS19140 0.31 -0.7 4,375,910 + DDA3937_RS19140 0.31 -1.3 4,375,939 - DDA3937_RS19140 0.34 -1.9 4,375,957 + DDA3937_RS19140 0.35 -0.7 4,375,957 + DDA3937_RS19140 0.35 -0.7 4,375,958 - DDA3937_RS19140 0.35 +0.3 4,375,960 + DDA3937_RS19140 0.35 -1.3 4,375,960 + DDA3937_RS19140 0.35 +0.9 4,376,007 + DDA3937_RS19140 0.38 -1.0 4,376,007 + DDA3937_RS19140 0.38 -1.7 4,376,007 + DDA3937_RS19140 0.38 +0.6 4,376,007 + DDA3937_RS19140 0.38 -2.9 4,376,018 + DDA3937_RS19140 0.39 -0.1 4,376,018 + DDA3937_RS19140 0.39 -0.7 4,376,170 + DDA3937_RS19140 0.50 -0.3 4,376,171 - DDA3937_RS19140 0.50 +0.3 4,376,172 + DDA3937_RS19140 0.50 -2.8 4,376,172 + DDA3937_RS19140 0.50 +1.3 4,376,172 + DDA3937_RS19140 0.50 -1.3 4,376,172 + DDA3937_RS19140 0.50 +0.9 4,376,173 - DDA3937_RS19140 0.51 +2.3 4,376,173 - -0.3 4,376,173 - DDA3937_RS19140 0.51 +0.3 4,376,173 - DDA3937_RS19140 0.51 +1.3 4,376,240 - DDA3937_RS19140 0.55 -1.9 4,376,241 + -0.3 4,376,255 + DDA3937_RS19140 0.56 -1.3 4,376,297 + DDA3937_RS19140 0.60 -0.7 4,376,298 - DDA3937_RS19140 0.60 -0.3 4,376,375 + DDA3937_RS19140 0.65 +0.3 4,376,479 + DDA3937_RS19140 0.73 -0.8 4,376,579 + DDA3937_RS19140 0.80 -1.9 4,376,586 - -0.7 4,376,586 - DDA3937_RS19140 0.81 -2.7 4,376,660 + DDA3937_RS19140 0.86 -0.5 4,376,760 - +0.3 4,376,781 + -0.9 4,376,803 + -1.9 4,376,804 - -1.5 4,376,852 - +0.6 4,376,869 + -1.9 4,376,870 - -0.3 4,376,870 - -0.5 4,376,874 - -0.0 4,376,874 - +0.5 4,376,925 - -0.3 4,376,955 - +1.1 4,377,077 + DDA3937_RS19145 0.28 -1.3 4,377,078 - DDA3937_RS19145 0.28 +1.6 4,377,078 - DDA3937_RS19145 0.28 +1.5 4,377,158 + DDA3937_RS19145 0.47 -2.0 4,377,158 + DDA3937_RS19145 0.47 +0.3 4,377,160 + DDA3937_RS19145 0.47 -0.7 4,377,160 + DDA3937_RS19145 0.47 -0.7 4,377,161 - DDA3937_RS19145 0.48 -2.2 4,377,161 - DDA3937_RS19145 0.48 -1.2 4,377,176 + DDA3937_RS19145 0.51 +0.7 4,377,176 + DDA3937_RS19145 0.51 +0.3 4,377,176 + DDA3937_RS19145 0.51 -2.6 4,377,177 - DDA3937_RS19145 0.51 -0.6 4,377,181 - DDA3937_RS19145 0.52 +0.0 4,377,187 + DDA3937_RS19145 0.54 +0.8 4,377,213 - DDA3937_RS19145 0.60 -0.3 4,377,250 + DDA3937_RS19145 0.69 +1.1 4,377,258 - DDA3937_RS19145 0.70 -0.1 4,377,343 + -1.0 4,377,344 - +0.5 4,377,381 + +0.6 4,377,382 - +0.3 4,377,382 - -0.7 4,377,382 - +1.3 4,377,385 + +0.6 4,377,385 + -0.5 4,377,386 - -0.5 4,377,386 - +0.5 4,377,386 - +0.9 4,377,390 + -0.7 4,377,425 - DDA3937_RS22500 0.16 -1.7 4,377,425 - DDA3937_RS22500 0.16 -1.0 4,377,425 - DDA3937_RS22500 0.16 +0.5 4,377,463 - DDA3937_RS22500 0.33 +0.3 4,377,463 - DDA3937_RS22500 0.33 +0.2 4,377,497 + DDA3937_RS22500 0.48 -0.4 4,377,497 + DDA3937_RS22500 0.48 +0.9 4,377,497 + DDA3937_RS22500 0.48 -1.9 4,377,497 + DDA3937_RS22500 0.48 -0.0 4,377,497 + DDA3937_RS22500 0.48 +0.3 4,377,497 + DDA3937_RS22500 0.48 -0.1 4,377,498 - DDA3937_RS22500 0.49 +1.1 4,377,498 - DDA3937_RS22500 0.49 -0.1 4,377,498 - DDA3937_RS22500 0.49 -0.6 4,377,507 + DDA3937_RS22500 0.53 -0.3 4,377,580 + DDA3937_RS22500 0.85 +0.1 4,377,580 + DDA3937_RS22500 0.85 +1.0 4,377,581 - DDA3937_RS22500 0.86 +0.1 4,377,581 - DDA3937_RS22500 0.86 -0.2 4,377,594 + -0.3 4,377,612 + -0.2 4,377,613 - +0.9 4,377,628 + -0.7 4,377,628 + -0.2 4,377,629 - -1.0 4,377,629 - +1.6 4,377,629 - -0.4 4,377,634 + -1.7 4,377,635 - -1.2 4,377,635 - +0.1 4,377,635 - +0.4 4,377,635 - -1.7 4,377,684 + -1.0 4,377,685 - -2.0 4,377,693 + +0.6 4,377,693 + -0.7 4,377,693 + +0.2 4,377,693 + +1.0 4,377,693 + -0.9 4,377,694 - +0.2 4,377,715 + DDA3937_RS19150 0.11 +1.6 4,377,715 + DDA3937_RS19150 0.11 +0.4 4,377,715 + DDA3937_RS19150 0.11 +0.5 4,377,716 - DDA3937_RS19150 0.11 -1.3 4,377,716 - DDA3937_RS19150 0.11 -0.4 4,377,716 - DDA3937_RS19150 0.11 +0.2 4,377,728 + DDA3937_RS19150 0.14 -0.4 4,377,729 - DDA3937_RS19150 0.14 -0.8 4,377,729 - DDA3937_RS19150 0.14 -1.4 4,377,729 - DDA3937_RS19150 0.14 -0.7 4,377,763 + DDA3937_RS19150 0.23 -2.4 4,377,776 - DDA3937_RS19150 0.27 +0.1 4,377,812 + DDA3937_RS19150 0.36 -0.9 4,377,812 + DDA3937_RS19150 0.36 -0.5 4,377,845 - DDA3937_RS19150 0.44 -2.4 4,377,845 - DDA3937_RS19150 0.44 +0.2
Or see this region's nucleotide sequence