Experiment: M9+glycerol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DDA3937_RS08515 and DDA3937_RS08520 overlap by 14 nucleotides DDA3937_RS08520 and DDA3937_RS08525 overlap by 8 nucleotides DDA3937_RS08525 and DDA3937_RS08530 are separated by 732 nucleotides
DDA3937_RS08515: DDA3937_RS08515 - 8-amino-7-oxononanoate synthase, at 1,925,086 to 1,926,240
_RS08515
DDA3937_RS08520: DDA3937_RS08520 - malonyl-ACP O-methyltransferase BioC, at 1,926,227 to 1,927,009
_RS08520
DDA3937_RS08525: DDA3937_RS08525 - ATP-dependent dethiobiotin synthetase BioD, at 1,927,002 to 1,927,682
_RS08525
DDA3937_RS08530: DDA3937_RS08530 - excinuclease ABC subunit B, at 1,928,415 to 1,930,427
_RS08530
Position (kb)
1927
1928 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1926.172 kb on - strand at 1926.226 kb on + strand at 1926.226 kb on + strand at 1926.226 kb on + strand at 1926.226 kb on + strand at 1926.227 kb on - strand at 1926.227 kb on - strand at 1926.227 kb on - strand at 1926.311 kb on + strand, within DDA3937_RS08520 at 1926.311 kb on + strand, within DDA3937_RS08520 at 1926.311 kb on + strand, within DDA3937_RS08520 at 1926.311 kb on + strand, within DDA3937_RS08520 at 1926.312 kb on - strand, within DDA3937_RS08520 at 1926.312 kb on - strand, within DDA3937_RS08520 at 1926.312 kb on - strand, within DDA3937_RS08520 at 1926.522 kb on + strand, within DDA3937_RS08520 at 1926.522 kb on + strand, within DDA3937_RS08520 at 1926.522 kb on + strand, within DDA3937_RS08520 at 1926.522 kb on + strand, within DDA3937_RS08520 at 1926.523 kb on - strand, within DDA3937_RS08520 at 1926.523 kb on - strand, within DDA3937_RS08520 at 1926.523 kb on - strand, within DDA3937_RS08520 at 1926.523 kb on - strand, within DDA3937_RS08520 at 1926.523 kb on - strand, within DDA3937_RS08520 at 1926.523 kb on - strand, within DDA3937_RS08520 at 1926.523 kb on - strand, within DDA3937_RS08520 at 1926.748 kb on + strand, within DDA3937_RS08520 at 1926.749 kb on - strand, within DDA3937_RS08520 at 1926.821 kb on + strand, within DDA3937_RS08520 at 1926.821 kb on + strand, within DDA3937_RS08520 at 1926.822 kb on - strand, within DDA3937_RS08520 at 1926.941 kb on + strand at 1926.941 kb on + strand at 1926.941 kb on + strand at 1926.942 kb on - strand at 1926.974 kb on + strand at 1926.975 kb on - strand at 1926.975 kb on - strand at 1926.975 kb on - strand at 1927.206 kb on + strand, within DDA3937_RS08525 at 1927.207 kb on - strand, within DDA3937_RS08525 at 1927.253 kb on + strand, within DDA3937_RS08525 at 1927.253 kb on + strand, within DDA3937_RS08525 at 1927.253 kb on + strand, within DDA3937_RS08525 at 1927.254 kb on - strand, within DDA3937_RS08525 at 1927.254 kb on - strand, within DDA3937_RS08525 at 1927.389 kb on - strand, within DDA3937_RS08525 at 1927.564 kb on - strand, within DDA3937_RS08525 at 1927.564 kb on - strand, within DDA3937_RS08525 at 1927.564 kb on - strand, within DDA3937_RS08525 at 1927.564 kb on - strand, within DDA3937_RS08525 at 1927.618 kb on - strand at 1927.621 kb on + strand at 1927.621 kb on + strand at 1927.654 kb on - strand at 1927.657 kb on + strand at 1927.657 kb on + strand at 1927.693 kb on + strand at 1927.803 kb on + strand at 1927.914 kb on + strand at 1927.917 kb on + strand at 1927.918 kb on - strand at 1927.964 kb on + strand at 1927.975 kb on - strand at 1927.975 kb on - strand at 1927.977 kb on + strand at 1928.120 kb on + strand at 1928.121 kb on - strand at 1928.141 kb on - strand at 1928.141 kb on - strand at 1928.148 kb on - strand at 1928.217 kb on + strand at 1928.277 kb on + strand at 1928.287 kb on - strand at 1928.290 kb on - strand at 1928.293 kb on + strand at 1928.294 kb on - strand at 1928.294 kb on - strand at 1928.294 kb on - strand at 1928.322 kb on - strand at 1928.324 kb on + strand at 1928.324 kb on + strand at 1928.324 kb on + strand at 1928.324 kb on + strand at 1928.324 kb on + strand at 1928.324 kb on + strand at 1928.324 kb on + strand at 1928.324 kb on + strand at 1928.324 kb on + strand at 1928.324 kb on + strand at 1928.324 kb on + strand at 1928.324 kb on + strand at 1928.324 kb on + strand at 1928.324 kb on + strand at 1928.324 kb on + strand at 1928.325 kb on - strand at 1928.325 kb on - strand at 1928.325 kb on - strand at 1928.325 kb on - strand at 1928.325 kb on - strand at 1928.325 kb on - strand at 1928.325 kb on - strand at 1928.325 kb on - strand at 1928.325 kb on - strand at 1928.325 kb on - strand at 1928.325 kb on - strand at 1928.410 kb on + strand at 1928.410 kb on + strand at 1928.421 kb on - strand at 1928.421 kb on - strand at 1928.421 kb on - strand at 1928.524 kb on + strand at 1928.547 kb on - strand at 1928.634 kb on + strand at 1928.635 kb on - strand, within DDA3937_RS08530 at 1928.635 kb on - strand, within DDA3937_RS08530 at 1928.676 kb on + strand, within DDA3937_RS08530 at 1928.676 kb on + strand, within DDA3937_RS08530
Per-strain Table
Position Strand Gene LocusTag Fraction M9+glycerol remove 1,926,172 - -2.1 1,926,226 + -1.8 1,926,226 + -1.6 1,926,226 + -0.5 1,926,226 + +0.0 1,926,227 - -0.1 1,926,227 - +0.0 1,926,227 - -2.0 1,926,311 + DDA3937_RS08520 0.11 -0.4 1,926,311 + DDA3937_RS08520 0.11 -1.1 1,926,311 + DDA3937_RS08520 0.11 -0.3 1,926,311 + DDA3937_RS08520 0.11 -1.0 1,926,312 - DDA3937_RS08520 0.11 -2.0 1,926,312 - DDA3937_RS08520 0.11 -1.3 1,926,312 - DDA3937_RS08520 0.11 +0.2 1,926,522 + DDA3937_RS08520 0.38 +1.5 1,926,522 + DDA3937_RS08520 0.38 -0.6 1,926,522 + DDA3937_RS08520 0.38 +0.3 1,926,522 + DDA3937_RS08520 0.38 -1.1 1,926,523 - DDA3937_RS08520 0.38 -0.4 1,926,523 - DDA3937_RS08520 0.38 -0.7 1,926,523 - DDA3937_RS08520 0.38 -0.6 1,926,523 - DDA3937_RS08520 0.38 +0.4 1,926,523 - DDA3937_RS08520 0.38 -0.4 1,926,523 - DDA3937_RS08520 0.38 -0.7 1,926,523 - DDA3937_RS08520 0.38 -0.6 1,926,748 + DDA3937_RS08520 0.67 -0.6 1,926,749 - DDA3937_RS08520 0.67 +0.3 1,926,821 + DDA3937_RS08520 0.76 +1.7 1,926,821 + DDA3937_RS08520 0.76 +0.0 1,926,822 - DDA3937_RS08520 0.76 -2.1 1,926,941 + -0.3 1,926,941 + -1.6 1,926,941 + -0.8 1,926,942 - -0.3 1,926,974 + -0.4 1,926,975 - -1.7 1,926,975 - +1.7 1,926,975 - -0.1 1,927,206 + DDA3937_RS08525 0.30 -1.8 1,927,207 - DDA3937_RS08525 0.30 -1.8 1,927,253 + DDA3937_RS08525 0.37 -1.3 1,927,253 + DDA3937_RS08525 0.37 -0.5 1,927,253 + DDA3937_RS08525 0.37 -0.8 1,927,254 - DDA3937_RS08525 0.37 -1.7 1,927,254 - DDA3937_RS08525 0.37 +0.1 1,927,389 - DDA3937_RS08525 0.57 -0.3 1,927,564 - DDA3937_RS08525 0.83 +0.1 1,927,564 - DDA3937_RS08525 0.83 -1.5 1,927,564 - DDA3937_RS08525 0.83 -1.1 1,927,564 - DDA3937_RS08525 0.83 -1.1 1,927,618 - -1.1 1,927,621 + -0.9 1,927,621 + +0.9 1,927,654 - -1.5 1,927,657 + -1.0 1,927,657 + -0.3 1,927,693 + -2.6 1,927,803 + -2.2 1,927,914 + +0.1 1,927,917 + -0.5 1,927,918 - -2.6 1,927,964 + +0.8 1,927,975 - +0.1 1,927,975 - +0.7 1,927,977 + -0.9 1,928,120 + -2.0 1,928,121 - -0.5 1,928,141 - -0.6 1,928,141 - -0.4 1,928,148 - -0.5 1,928,217 + -1.0 1,928,277 + -1.3 1,928,287 - -0.5 1,928,290 - +2.0 1,928,293 + -1.6 1,928,294 - -1.3 1,928,294 - -0.1 1,928,294 - +0.9 1,928,322 - +0.0 1,928,324 + -1.0 1,928,324 + +1.3 1,928,324 + -0.7 1,928,324 + -0.8 1,928,324 + -0.8 1,928,324 + +0.6 1,928,324 + +0.9 1,928,324 + -0.7 1,928,324 + -0.2 1,928,324 + +0.5 1,928,324 + -0.4 1,928,324 + +0.1 1,928,324 + -2.1 1,928,324 + +0.7 1,928,324 + +0.5 1,928,325 - -0.3 1,928,325 - -0.5 1,928,325 - -0.0 1,928,325 - -1.8 1,928,325 - -1.3 1,928,325 - +0.1 1,928,325 - -0.3 1,928,325 - -1.0 1,928,325 - -2.1 1,928,325 - -0.1 1,928,325 - -2.7 1,928,410 + +0.5 1,928,410 + +0.0 1,928,421 - +0.2 1,928,421 - -0.4 1,928,421 - +0.1 1,928,524 + -1.3 1,928,547 - -0.9 1,928,634 + +1.2 1,928,635 - DDA3937_RS08530 0.11 -2.4 1,928,635 - DDA3937_RS08530 0.11 +0.4 1,928,676 + DDA3937_RS08530 0.13 -0.3 1,928,676 + DDA3937_RS08530 0.13 -0.4
Or see this region's nucleotide sequence