Experiment: PDB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DDA3937_RS12440 and DDA3937_RS12445 overlap by 4 nucleotides DDA3937_RS12445 and DDA3937_RS12450 overlap by 14 nucleotides DDA3937_RS12450 and DDA3937_RS12455 are separated by 15 nucleotides
DDA3937_RS12440: DDA3937_RS12440 - peptide ABC transporter substrate-binding protein SapA, at 2,831,535 to 2,833,247
_RS12440
DDA3937_RS12445: DDA3937_RS12445 - peptide ABC transporter permease SapB, at 2,833,244 to 2,834,209
_RS12445
DDA3937_RS12450: DDA3937_RS12450 - peptide ABC transporter permease SapC, at 2,834,196 to 2,835,086
_RS12450
DDA3937_RS12455: DDA3937_RS12455 - peptide ABC transporter ATP-binding protein SapD, at 2,835,102 to 2,836,094
_RS12455
Position (kb)
2834
2835
2836 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2833.290 kb on + strand at 2833.325 kb on + strand at 2833.325 kb on + strand at 2833.325 kb on + strand at 2833.326 kb on - strand at 2833.328 kb on + strand at 2833.329 kb on - strand at 2833.783 kb on - strand, within DDA3937_RS12445 at 2833.858 kb on + strand, within DDA3937_RS12445 at 2833.883 kb on + strand, within DDA3937_RS12445 at 2834.032 kb on + strand, within DDA3937_RS12445 at 2834.033 kb on - strand, within DDA3937_RS12445 at 2834.137 kb on + strand at 2834.138 kb on - strand at 2834.185 kb on + strand at 2834.185 kb on + strand at 2834.195 kb on + strand at 2834.196 kb on - strand at 2834.214 kb on + strand at 2834.214 kb on + strand at 2834.215 kb on - strand at 2834.406 kb on + strand, within DDA3937_RS12450 at 2834.587 kb on + strand, within DDA3937_RS12450 at 2834.641 kb on + strand, within DDA3937_RS12450 at 2834.761 kb on - strand at 2834.761 kb on - strand, within DDA3937_RS12450 at 2834.804 kb on + strand, within DDA3937_RS12450 at 2834.828 kb on + strand, within DDA3937_RS12450 at 2834.828 kb on + strand, within DDA3937_RS12450 at 2834.973 kb on + strand, within DDA3937_RS12450 at 2834.974 kb on - strand, within DDA3937_RS12450 at 2835.354 kb on - strand, within DDA3937_RS12455
Per-strain Table
Position Strand Gene LocusTag Fraction PDB remove 2,833,290 + +0.5 2,833,325 + +1.4 2,833,325 + -1.3 2,833,325 + -0.6 2,833,326 - +0.2 2,833,328 + -0.5 2,833,329 - +0.9 2,833,783 - DDA3937_RS12445 0.56 -1.0 2,833,858 + DDA3937_RS12445 0.64 +2.5 2,833,883 + DDA3937_RS12445 0.66 -1.0 2,834,032 + DDA3937_RS12445 0.82 -0.8 2,834,033 - DDA3937_RS12445 0.82 -1.5 2,834,137 + -2.0 2,834,138 - -0.2 2,834,185 + -3.3 2,834,185 + -0.7 2,834,195 + -1.3 2,834,196 - -0.8 2,834,214 + -1.8 2,834,214 + +1.5 2,834,215 - +0.8 2,834,406 + DDA3937_RS12450 0.24 -1.5 2,834,587 + DDA3937_RS12450 0.44 -1.0 2,834,641 + DDA3937_RS12450 0.50 -1.2 2,834,761 - -0.5 2,834,761 - DDA3937_RS12450 0.63 +1.0 2,834,804 + DDA3937_RS12450 0.68 -0.8 2,834,828 + DDA3937_RS12450 0.71 -0.8 2,834,828 + DDA3937_RS12450 0.71 -0.8 2,834,973 + DDA3937_RS12450 0.87 -2.0 2,834,974 - DDA3937_RS12450 0.87 -0.8 2,835,354 - DDA3937_RS12455 0.25 -1.8
Or see this region's nucleotide sequence