Experiment: PDB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DDA3937_RS04115 and DDA3937_RS04120 are separated by 107 nucleotides DDA3937_RS04120 and DDA3937_RS04125 overlap by 23 nucleotides DDA3937_RS04125 and DDA3937_RS04130 are separated by 1 nucleotides DDA3937_RS04130 and DDA3937_RS04135 are separated by 3 nucleotides
DDA3937_RS04115: DDA3937_RS04115 - site-specific integrase, at 915,452 to 916,543
_RS04115
DDA3937_RS04120: DDA3937_RS04120 - ASCH domain-containing protein, at 916,651 to 917,022
_RS04120
DDA3937_RS04125: DDA3937_RS04125 - acetyltransferase, at 917,000 to 918,073
_RS04125
DDA3937_RS04130: DDA3937_RS04130 - hypothetical protein, at 918,075 to 918,857
_RS04130
DDA3937_RS04135: DDA3937_RS04135 - helix-turn-helix domain-containing protein, at 918,861 to 919,712
_RS04135
Position (kb) 916
917
918
919 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 916.001 kb on + strand, within DDA3937_RS04115 at 916.040 kb on + strand, within DDA3937_RS04115 at 916.040 kb on + strand, within DDA3937_RS04115 at 916.040 kb on + strand, within DDA3937_RS04115 at 916.041 kb on - strand, within DDA3937_RS04115 at 916.122 kb on - strand, within DDA3937_RS04115 at 916.283 kb on - strand, within DDA3937_RS04115 at 916.307 kb on - strand, within DDA3937_RS04115 at 916.366 kb on + strand, within DDA3937_RS04115 at 916.453 kb on + strand at 916.498 kb on - strand at 916.531 kb on + strand at 916.531 kb on + strand at 916.532 kb on - strand at 916.543 kb on + strand at 916.543 kb on + strand at 916.543 kb on + strand at 916.544 kb on - strand at 916.544 kb on - strand at 916.544 kb on - strand at 916.544 kb on - strand at 916.556 kb on + strand at 916.557 kb on - strand at 916.557 kb on - strand at 916.571 kb on + strand at 916.571 kb on + strand at 916.571 kb on + strand at 916.571 kb on + strand at 916.571 kb on + strand at 916.571 kb on + strand at 916.571 kb on + strand at 916.572 kb on - strand at 916.572 kb on - strand at 916.572 kb on - strand at 916.575 kb on + strand at 916.575 kb on + strand at 916.575 kb on + strand at 916.576 kb on - strand at 916.576 kb on - strand at 916.576 kb on - strand at 916.576 kb on - strand at 916.602 kb on + strand at 916.602 kb on + strand at 916.602 kb on + strand at 916.602 kb on + strand at 916.602 kb on + strand at 916.602 kb on + strand at 916.602 kb on + strand at 916.602 kb on + strand at 916.602 kb on + strand at 916.602 kb on + strand at 916.602 kb on + strand at 916.602 kb on + strand at 916.603 kb on - strand at 916.603 kb on - strand at 916.603 kb on - strand at 916.603 kb on - strand at 916.603 kb on - strand at 916.603 kb on - strand at 916.652 kb on + strand at 916.652 kb on + strand at 916.653 kb on - strand at 916.653 kb on - strand at 917.015 kb on - strand at 917.208 kb on - strand, within DDA3937_RS04125 at 917.332 kb on - strand, within DDA3937_RS04125 at 917.332 kb on - strand, within DDA3937_RS04125 at 917.397 kb on - strand, within DDA3937_RS04125 at 917.443 kb on - strand, within DDA3937_RS04125 at 917.459 kb on - strand, within DDA3937_RS04125 at 917.459 kb on - strand, within DDA3937_RS04125 at 917.565 kb on + strand, within DDA3937_RS04125 at 917.700 kb on + strand, within DDA3937_RS04125 at 917.701 kb on - strand, within DDA3937_RS04125 at 917.839 kb on + strand, within DDA3937_RS04125 at 917.839 kb on + strand, within DDA3937_RS04125 at 917.840 kb on - strand, within DDA3937_RS04125 at 917.870 kb on + strand, within DDA3937_RS04125 at 917.938 kb on - strand, within DDA3937_RS04125 at 917.938 kb on - strand, within DDA3937_RS04125 at 918.150 kb on + strand at 918.170 kb on + strand, within DDA3937_RS04130 at 918.202 kb on + strand, within DDA3937_RS04130 at 918.506 kb on + strand, within DDA3937_RS04130 at 918.830 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction PDB remove 916,001 + DDA3937_RS04115 0.50 -0.3 916,040 + DDA3937_RS04115 0.54 -1.0 916,040 + DDA3937_RS04115 0.54 -0.9 916,040 + DDA3937_RS04115 0.54 -3.1 916,041 - DDA3937_RS04115 0.54 +0.4 916,122 - DDA3937_RS04115 0.61 -1.6 916,283 - DDA3937_RS04115 0.76 +0.1 916,307 - DDA3937_RS04115 0.78 -0.3 916,366 + DDA3937_RS04115 0.84 -3.3 916,453 + -0.5 916,498 - -1.5 916,531 + -2.7 916,531 + -1.2 916,532 - -0.8 916,543 + -2.4 916,543 + -2.8 916,543 + -0.8 916,544 - -0.4 916,544 - -0.3 916,544 - -2.5 916,544 - +2.0 916,556 + -1.3 916,557 - -1.7 916,557 - -1.1 916,571 + -0.3 916,571 + -0.7 916,571 + -0.4 916,571 + -0.0 916,571 + -2.0 916,571 + -1.0 916,571 + -1.3 916,572 - -0.6 916,572 - +1.4 916,572 - -2.7 916,575 + -2.4 916,575 + -0.5 916,575 + -1.0 916,576 - +0.4 916,576 - -0.6 916,576 - +0.4 916,576 - -1.7 916,602 + -1.0 916,602 + +0.7 916,602 + +0.5 916,602 + +1.2 916,602 + -2.0 916,602 + -1.0 916,602 + +0.2 916,602 + +1.2 916,602 + -0.6 916,602 + -2.5 916,602 + -1.3 916,602 + -1.2 916,603 - -0.8 916,603 - -1.0 916,603 - +0.7 916,603 - +0.6 916,603 - -1.2 916,603 - -2.3 916,652 + -0.0 916,652 + -1.8 916,653 - -0.8 916,653 - -1.8 917,015 - -1.0 917,208 - DDA3937_RS04125 0.19 -2.3 917,332 - DDA3937_RS04125 0.31 -1.1 917,332 - DDA3937_RS04125 0.31 -1.0 917,397 - DDA3937_RS04125 0.37 -1.5 917,443 - DDA3937_RS04125 0.41 -1.3 917,459 - DDA3937_RS04125 0.43 +0.7 917,459 - DDA3937_RS04125 0.43 -0.0 917,565 + DDA3937_RS04125 0.53 -0.0 917,700 + DDA3937_RS04125 0.65 -1.7 917,701 - DDA3937_RS04125 0.65 -1.2 917,839 + DDA3937_RS04125 0.78 +0.4 917,839 + DDA3937_RS04125 0.78 +1.3 917,840 - DDA3937_RS04125 0.78 -1.7 917,870 + DDA3937_RS04125 0.81 -2.8 917,938 - DDA3937_RS04125 0.87 +0.8 917,938 - DDA3937_RS04125 0.87 -0.3 918,150 + -2.3 918,170 + DDA3937_RS04130 0.12 -1.5 918,202 + DDA3937_RS04130 0.16 -1.8 918,506 + DDA3937_RS04130 0.55 -1.6 918,830 + -0.2
Or see this region's nucleotide sequence