Experiment: PDB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DDA3937_RS02405 and DDA3937_RS02410 are separated by 319 nucleotides DDA3937_RS02410 and DDA3937_RS02415 are separated by 13 nucleotides DDA3937_RS02415 and DDA3937_RS02420 are separated by 69 nucleotides
DDA3937_RS02405: DDA3937_RS02405 - cell division protein ZapA, at 533,044 to 533,373
_RS02405
DDA3937_RS02410: DDA3937_RS02410 - 5-formyltetrahydrofolate cyclo-ligase, at 533,693 to 534,307
_RS02410
DDA3937_RS02415: DDA3937_RS02415 - multifunctional transcriptional regulator/nicotinamide-nucleotide adenylyltransferase/ribosylnicotinamide kinase NadR, at 534,321 to 535,577
_RS02415
DDA3937_RS02420: DDA3937_RS02420 - DNA repair protein RadA, at 535,647 to 537,032
_RS02420
Position (kb) 534
535
536 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 533.341 kb on - strand at 533.396 kb on - strand at 533.433 kb on + strand at 533.433 kb on + strand at 533.433 kb on + strand at 533.433 kb on + strand at 533.433 kb on + strand at 533.433 kb on + strand at 533.433 kb on + strand at 533.434 kb on - strand at 533.528 kb on + strand at 533.529 kb on - strand at 533.609 kb on + strand at 533.645 kb on + strand at 533.645 kb on + strand at 533.646 kb on - strand at 533.650 kb on - strand at 533.674 kb on + strand at 533.674 kb on + strand at 533.674 kb on + strand at 533.675 kb on - strand at 533.675 kb on - strand at 533.675 kb on - strand at 533.739 kb on + strand at 533.804 kb on - strand, within DDA3937_RS02410 at 533.948 kb on + strand, within DDA3937_RS02410 at 533.948 kb on + strand, within DDA3937_RS02410 at 533.948 kb on + strand, within DDA3937_RS02410 at 533.949 kb on - strand, within DDA3937_RS02410 at 533.949 kb on - strand, within DDA3937_RS02410 at 534.158 kb on + strand, within DDA3937_RS02410 at 534.246 kb on + strand at 534.277 kb on + strand at 534.277 kb on + strand at 534.277 kb on + strand at 534.323 kb on - strand at 534.406 kb on + strand at 534.431 kb on - strand at 534.431 kb on - strand at 534.440 kb on + strand at 534.440 kb on + strand at 534.440 kb on + strand at 534.441 kb on - strand at 534.455 kb on + strand, within DDA3937_RS02415 at 534.456 kb on - strand, within DDA3937_RS02415 at 534.456 kb on - strand, within DDA3937_RS02415 at 534.456 kb on - strand, within DDA3937_RS02415 at 534.568 kb on + strand, within DDA3937_RS02415 at 534.614 kb on - strand, within DDA3937_RS02415 at 534.614 kb on - strand, within DDA3937_RS02415 at 534.676 kb on + strand, within DDA3937_RS02415 at 534.677 kb on - strand, within DDA3937_RS02415 at 534.697 kb on + strand, within DDA3937_RS02415 at 534.698 kb on - strand, within DDA3937_RS02415 at 534.779 kb on - strand, within DDA3937_RS02415 at 534.779 kb on - strand, within DDA3937_RS02415 at 534.779 kb on - strand, within DDA3937_RS02415 at 534.914 kb on + strand at 534.914 kb on + strand, within DDA3937_RS02415 at 534.915 kb on - strand, within DDA3937_RS02415 at 534.915 kb on - strand, within DDA3937_RS02415 at 534.916 kb on + strand, within DDA3937_RS02415 at 534.916 kb on + strand, within DDA3937_RS02415 at 534.916 kb on + strand, within DDA3937_RS02415 at 534.916 kb on + strand, within DDA3937_RS02415 at 534.916 kb on + strand, within DDA3937_RS02415 at 534.917 kb on - strand, within DDA3937_RS02415 at 534.917 kb on - strand, within DDA3937_RS02415 at 534.917 kb on - strand, within DDA3937_RS02415 at 534.917 kb on - strand, within DDA3937_RS02415 at 534.917 kb on - strand, within DDA3937_RS02415 at 534.917 kb on - strand, within DDA3937_RS02415 at 534.917 kb on - strand, within DDA3937_RS02415 at 535.022 kb on - strand, within DDA3937_RS02415 at 535.051 kb on + strand, within DDA3937_RS02415 at 535.051 kb on + strand, within DDA3937_RS02415 at 535.085 kb on + strand, within DDA3937_RS02415 at 535.085 kb on + strand, within DDA3937_RS02415 at 535.085 kb on + strand, within DDA3937_RS02415 at 535.086 kb on - strand, within DDA3937_RS02415 at 535.086 kb on - strand, within DDA3937_RS02415 at 535.296 kb on + strand, within DDA3937_RS02415 at 535.296 kb on + strand, within DDA3937_RS02415 at 535.297 kb on - strand, within DDA3937_RS02415 at 535.297 kb on - strand, within DDA3937_RS02415 at 535.297 kb on - strand, within DDA3937_RS02415 at 535.297 kb on - strand, within DDA3937_RS02415 at 535.297 kb on - strand, within DDA3937_RS02415 at 535.297 kb on - strand at 535.334 kb on - strand, within DDA3937_RS02415 at 535.358 kb on - strand, within DDA3937_RS02415 at 535.371 kb on + strand, within DDA3937_RS02415 at 535.371 kb on + strand, within DDA3937_RS02415 at 535.372 kb on - strand, within DDA3937_RS02415 at 535.372 kb on - strand, within DDA3937_RS02415 at 535.397 kb on - strand, within DDA3937_RS02415 at 535.434 kb on + strand, within DDA3937_RS02415 at 535.434 kb on + strand, within DDA3937_RS02415 at 535.434 kb on + strand, within DDA3937_RS02415 at 535.486 kb on + strand at 535.567 kb on + strand at 535.611 kb on + strand at 535.611 kb on + strand at 535.611 kb on + strand at 535.612 kb on - strand at 535.612 kb on - strand at 535.612 kb on - strand at 535.612 kb on - strand at 535.612 kb on - strand at 535.617 kb on - strand at 535.698 kb on + strand at 535.699 kb on - strand at 535.699 kb on - strand at 535.784 kb on + strand at 535.784 kb on + strand at 535.784 kb on + strand at 535.785 kb on - strand at 536.124 kb on + strand, within DDA3937_RS02420 at 536.124 kb on + strand, within DDA3937_RS02420 at 536.124 kb on + strand, within DDA3937_RS02420 at 536.125 kb on - strand, within DDA3937_RS02420 at 536.125 kb on - strand, within DDA3937_RS02420 at 536.125 kb on - strand, within DDA3937_RS02420 at 536.125 kb on - strand, within DDA3937_RS02420 at 536.223 kb on + strand, within DDA3937_RS02420 at 536.322 kb on - strand, within DDA3937_RS02420 at 536.322 kb on - strand, within DDA3937_RS02420 at 536.422 kb on + strand, within DDA3937_RS02420
Per-strain Table
Position Strand Gene LocusTag Fraction PDB remove 533,341 - -1.0 533,396 - -2.2 533,433 + +0.1 533,433 + -2.5 533,433 + +1.7 533,433 + -2.1 533,433 + +0.6 533,433 + +2.8 533,433 + -1.6 533,434 - -0.0 533,528 + +2.6 533,529 - +2.0 533,609 + +3.1 533,645 + -0.0 533,645 + -1.0 533,646 - -0.1 533,650 - +2.4 533,674 + -1.9 533,674 + -1.1 533,674 + -1.0 533,675 - +0.1 533,675 - -1.9 533,675 - +0.7 533,739 + +0.1 533,804 - DDA3937_RS02410 0.18 +0.6 533,948 + DDA3937_RS02410 0.41 -0.1 533,948 + DDA3937_RS02410 0.41 +1.3 533,948 + DDA3937_RS02410 0.41 +1.3 533,949 - DDA3937_RS02410 0.42 +1.9 533,949 - DDA3937_RS02410 0.42 +1.6 534,158 + DDA3937_RS02410 0.76 -2.4 534,246 + -1.9 534,277 + -2.0 534,277 + +3.5 534,277 + +1.6 534,323 - -1.1 534,406 + +3.3 534,431 - +0.5 534,431 - -1.6 534,440 + -0.0 534,440 + -1.0 534,440 + -0.8 534,441 - -1.2 534,455 + DDA3937_RS02415 0.11 -0.0 534,456 - DDA3937_RS02415 0.11 +1.4 534,456 - DDA3937_RS02415 0.11 +2.4 534,456 - DDA3937_RS02415 0.11 +3.2 534,568 + DDA3937_RS02415 0.20 -2.4 534,614 - DDA3937_RS02415 0.23 -2.3 534,614 - DDA3937_RS02415 0.23 +1.6 534,676 + DDA3937_RS02415 0.28 +0.6 534,677 - DDA3937_RS02415 0.28 +0.8 534,697 + DDA3937_RS02415 0.30 -2.1 534,698 - DDA3937_RS02415 0.30 +1.7 534,779 - DDA3937_RS02415 0.36 +1.6 534,779 - DDA3937_RS02415 0.36 -2.9 534,779 - DDA3937_RS02415 0.36 -1.1 534,914 + -0.4 534,914 + DDA3937_RS02415 0.47 -1.2 534,915 - DDA3937_RS02415 0.47 +1.8 534,915 - DDA3937_RS02415 0.47 -0.1 534,916 + DDA3937_RS02415 0.47 +2.2 534,916 + DDA3937_RS02415 0.47 +0.8 534,916 + DDA3937_RS02415 0.47 -0.9 534,916 + DDA3937_RS02415 0.47 -3.0 534,916 + DDA3937_RS02415 0.47 +1.1 534,917 - DDA3937_RS02415 0.47 -2.0 534,917 - DDA3937_RS02415 0.47 -0.6 534,917 - DDA3937_RS02415 0.47 +1.9 534,917 - DDA3937_RS02415 0.47 -2.0 534,917 - DDA3937_RS02415 0.47 -1.3 534,917 - DDA3937_RS02415 0.47 -0.8 534,917 - DDA3937_RS02415 0.47 +1.2 535,022 - DDA3937_RS02415 0.56 -1.0 535,051 + DDA3937_RS02415 0.58 +1.5 535,051 + DDA3937_RS02415 0.58 -0.1 535,085 + DDA3937_RS02415 0.61 -1.0 535,085 + DDA3937_RS02415 0.61 -2.5 535,085 + DDA3937_RS02415 0.61 -0.8 535,086 - DDA3937_RS02415 0.61 -0.8 535,086 - DDA3937_RS02415 0.61 +3.7 535,296 + DDA3937_RS02415 0.78 -1.8 535,296 + DDA3937_RS02415 0.78 +2.0 535,297 - DDA3937_RS02415 0.78 -1.3 535,297 - DDA3937_RS02415 0.78 +1.1 535,297 - DDA3937_RS02415 0.78 +1.7 535,297 - DDA3937_RS02415 0.78 +2.1 535,297 - DDA3937_RS02415 0.78 -2.7 535,297 - +0.6 535,334 - DDA3937_RS02415 0.81 -0.8 535,358 - DDA3937_RS02415 0.82 +1.7 535,371 + DDA3937_RS02415 0.84 -2.8 535,371 + DDA3937_RS02415 0.84 +1.6 535,372 - DDA3937_RS02415 0.84 +1.4 535,372 - DDA3937_RS02415 0.84 -2.0 535,397 - DDA3937_RS02415 0.86 +2.1 535,434 + DDA3937_RS02415 0.89 +1.1 535,434 + DDA3937_RS02415 0.89 -0.8 535,434 + DDA3937_RS02415 0.89 +2.9 535,486 + -0.7 535,567 + +2.3 535,611 + -2.8 535,611 + +1.0 535,611 + +2.3 535,612 - -0.0 535,612 - +1.4 535,612 - -0.4 535,612 - -1.1 535,612 - -3.0 535,617 - +1.8 535,698 + +0.8 535,699 - -0.0 535,699 - +1.3 535,784 + -0.8 535,784 + -1.4 535,784 + -0.1 535,785 - -0.6 536,124 + DDA3937_RS02420 0.34 +0.4 536,124 + DDA3937_RS02420 0.34 -0.9 536,124 + DDA3937_RS02420 0.34 -1.6 536,125 - DDA3937_RS02420 0.34 -0.4 536,125 - DDA3937_RS02420 0.34 +0.7 536,125 - DDA3937_RS02420 0.34 -0.3 536,125 - DDA3937_RS02420 0.34 -0.0 536,223 + DDA3937_RS02420 0.42 -1.4 536,322 - DDA3937_RS02420 0.49 +0.7 536,322 - DDA3937_RS02420 0.49 -0.0 536,422 + DDA3937_RS02420 0.56 +0.7
Or see this region's nucleotide sequence