Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DDA3937_RS20105 and DDA3937_RS20110 are separated by 184 nucleotides DDA3937_RS20110 and DDA3937_RS20115 are separated by 40 nucleotides
DDA3937_RS20105: DDA3937_RS20105 - DNA helicase Rep, at 4,578,465 to 4,580,489
_RS20105
DDA3937_RS20110: DDA3937_RS20110 - peptidylprolyl isomerase, at 4,580,674 to 4,580,955
_RS20110
DDA3937_RS20115: DDA3937_RS20115 - trifunctional transcriptional regulator/proline dehydrogenase/L-glutamate gamma-semialdehyde dehydrogenase, at 4,580,996 to 4,584,985
_RS20115
Position (kb)
4580
4581 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4580.528 kb on - strand at 4580.544 kb on - strand at 4580.544 kb on - strand at 4580.625 kb on + strand at 4580.625 kb on + strand at 4580.626 kb on - strand at 4580.626 kb on - strand at 4580.626 kb on - strand at 4580.670 kb on + strand at 4580.670 kb on + strand at 4580.670 kb on + strand at 4580.670 kb on + strand at 4580.670 kb on + strand at 4580.670 kb on + strand at 4580.670 kb on + strand at 4580.671 kb on - strand at 4580.671 kb on - strand at 4580.671 kb on - strand at 4580.671 kb on - strand at 4580.671 kb on - strand at 4580.671 kb on - strand at 4580.671 kb on - strand at 4580.673 kb on + strand at 4580.674 kb on - strand at 4580.674 kb on - strand at 4580.682 kb on + strand at 4580.734 kb on - strand, within DDA3937_RS20110 at 4580.774 kb on + strand, within DDA3937_RS20110 at 4580.775 kb on - strand, within DDA3937_RS20110 at 4580.945 kb on - strand at 4580.949 kb on + strand at 4580.954 kb on - strand at 4580.994 kb on + strand at 4580.994 kb on + strand at 4580.994 kb on + strand at 4580.994 kb on + strand at 4580.994 kb on + strand at 4580.995 kb on - strand at 4580.995 kb on - strand at 4580.995 kb on - strand at 4580.995 kb on - strand at 4580.997 kb on + strand at 4581.047 kb on - strand at 4581.080 kb on + strand at 4581.081 kb on - strand at 4581.403 kb on + strand, within DDA3937_RS20115 at 4581.404 kb on - strand, within DDA3937_RS20115 at 4581.405 kb on + strand, within DDA3937_RS20115 at 4581.405 kb on + strand, within DDA3937_RS20115 at 4581.405 kb on + strand, within DDA3937_RS20115 at 4581.405 kb on + strand, within DDA3937_RS20115 at 4581.406 kb on - strand, within DDA3937_RS20115 at 4581.406 kb on - strand, within DDA3937_RS20115 at 4581.652 kb on - strand, within DDA3937_RS20115 at 4581.652 kb on - strand, within DDA3937_RS20115 at 4581.733 kb on + strand, within DDA3937_RS20115 at 4581.733 kb on + strand, within DDA3937_RS20115 at 4581.747 kb on + strand, within DDA3937_RS20115 at 4581.747 kb on + strand, within DDA3937_RS20115 at 4581.747 kb on + strand, within DDA3937_RS20115 at 4581.748 kb on - strand, within DDA3937_RS20115 at 4581.748 kb on - strand, within DDA3937_RS20115 at 4581.748 kb on - strand, within DDA3937_RS20115 at 4581.748 kb on - strand, within DDA3937_RS20115 at 4581.871 kb on + strand, within DDA3937_RS20115 at 4581.929 kb on + strand, within DDA3937_RS20115 at 4581.930 kb on - strand, within DDA3937_RS20115
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 4,580,528 - +1.5 4,580,544 - -0.2 4,580,544 - -1.3 4,580,625 + -0.4 4,580,625 + +0.7 4,580,626 - -0.6 4,580,626 - +0.0 4,580,626 - -0.1 4,580,670 + -0.2 4,580,670 + +0.1 4,580,670 + +0.2 4,580,670 + +0.5 4,580,670 + +1.3 4,580,670 + -0.6 4,580,670 + -0.4 4,580,671 - +0.3 4,580,671 - -0.9 4,580,671 - -1.2 4,580,671 - -1.3 4,580,671 - +0.6 4,580,671 - -0.5 4,580,671 - -0.4 4,580,673 + -0.1 4,580,674 - -0.7 4,580,674 - +0.5 4,580,682 + -1.0 4,580,734 - DDA3937_RS20110 0.21 -0.3 4,580,774 + DDA3937_RS20110 0.35 +0.7 4,580,775 - DDA3937_RS20110 0.36 +1.0 4,580,945 - -0.6 4,580,949 + +1.3 4,580,954 - +0.2 4,580,994 + -0.9 4,580,994 + -2.3 4,580,994 + +0.2 4,580,994 + -0.1 4,580,994 + -0.3 4,580,995 - +0.5 4,580,995 - +0.9 4,580,995 - +0.7 4,580,995 - -0.3 4,580,997 + -1.0 4,581,047 - -0.3 4,581,080 + +0.1 4,581,081 - +0.3 4,581,403 + DDA3937_RS20115 0.10 +0.2 4,581,404 - DDA3937_RS20115 0.10 -0.7 4,581,405 + DDA3937_RS20115 0.10 -1.2 4,581,405 + DDA3937_RS20115 0.10 +0.6 4,581,405 + DDA3937_RS20115 0.10 -0.6 4,581,405 + DDA3937_RS20115 0.10 +0.6 4,581,406 - DDA3937_RS20115 0.10 +0.6 4,581,406 - DDA3937_RS20115 0.10 -1.0 4,581,652 - DDA3937_RS20115 0.16 -0.6 4,581,652 - DDA3937_RS20115 0.16 -1.4 4,581,733 + DDA3937_RS20115 0.18 +0.4 4,581,733 + DDA3937_RS20115 0.18 -0.6 4,581,747 + DDA3937_RS20115 0.19 -0.4 4,581,747 + DDA3937_RS20115 0.19 -2.6 4,581,747 + DDA3937_RS20115 0.19 +0.6 4,581,748 - DDA3937_RS20115 0.19 -2.0 4,581,748 - DDA3937_RS20115 0.19 +0.8 4,581,748 - DDA3937_RS20115 0.19 +1.9 4,581,748 - DDA3937_RS20115 0.19 +0.9 4,581,871 + DDA3937_RS20115 0.22 +0.4 4,581,929 + DDA3937_RS20115 0.23 -1.5 4,581,930 - DDA3937_RS20115 0.23 +0.1
Or see this region's nucleotide sequence