Experiment: Upstate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS05505 and DZA65_RS05510 are separated by 148 nucleotides DZA65_RS05510 and DZA65_RS05515 are separated by 3 nucleotides DZA65_RS05515 and DZA65_RS05520 are separated by 3 nucleotides
DZA65_RS05505: DZA65_RS05505 - UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase, at 1,133,281 to 1,134,303
_RS05505
DZA65_RS05510: DZA65_RS05510 - 3-hydroxyacyl-ACP dehydratase FabZ, at 1,134,452 to 1,134,907
_RS05510
DZA65_RS05515: DZA65_RS05515 - acyl-ACP--UDP-N-acetylglucosamine O-acyltransferase, at 1,134,911 to 1,135,699
_RS05515
DZA65_RS05520: DZA65_RS05520 - lipid-A-disaccharide synthase, at 1,135,703 to 1,136,851
_RS05520
Position (kb)
1134
1135
1136 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1134.285 kb on + strand at 1134.336 kb on + strand at 1134.336 kb on + strand at 1134.336 kb on + strand at 1134.336 kb on + strand at 1134.336 kb on + strand at 1134.378 kb on + strand at 1134.378 kb on + strand at 1134.378 kb on + strand at 1134.378 kb on + strand at 1134.378 kb on + strand at 1134.415 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Upstate remove 1,134,285 + +1.3 1,134,336 + -1.6 1,134,336 + -0.4 1,134,336 + -0.9 1,134,336 + -0.8 1,134,336 + -2.0 1,134,378 + +0.1 1,134,378 + +0.4 1,134,378 + -1.5 1,134,378 + +0.0 1,134,378 + +2.3 1,134,415 + -0.6
Or see this region's nucleotide sequence