Experiment: Upstate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS12545 and DZA65_RS12550 are separated by 124 nucleotides DZA65_RS12550 and DZA65_RS12555 overlap by 4 nucleotides DZA65_RS12555 and DZA65_RS12560 are separated by 187 nucleotides DZA65_RS12560 and DZA65_RS12565 overlap by 1 nucleotides DZA65_RS12565 and DZA65_RS12570 are separated by 168 nucleotides
DZA65_RS12545: DZA65_RS12545 - phage repressor protein CI, at 2,689,994 to 2,690,836
_RS12545
DZA65_RS12550: DZA65_RS12550 - hypothetical protein, at 2,690,961 to 2,691,431
_RS12550
DZA65_RS12555: DZA65_RS12555 - hypothetical protein, at 2,691,428 to 2,691,982
_RS12555
DZA65_RS12560: DZA65_RS12560 - DUF2732 family protein, at 2,692,170 to 2,692,382
_RS12560
DZA65_RS12565: DZA65_RS12565 - hypothetical protein, at 2,692,382 to 2,692,606
_RS12565
DZA65_RS12570: DZA65_RS12570 - replication endonuclease, at 2,692,775 to 2,694,955
_RS12570
Position (kb)
2691
2692 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2690.929 kb on - strand at 2690.953 kb on - strand at 2690.983 kb on + strand at 2690.984 kb on - strand at 2691.074 kb on + strand, within DZA65_RS12550 at 2691.074 kb on + strand, within DZA65_RS12550 at 2691.074 kb on + strand, within DZA65_RS12550 at 2691.074 kb on + strand, within DZA65_RS12550 at 2691.075 kb on - strand, within DZA65_RS12550 at 2691.077 kb on - strand, within DZA65_RS12550 at 2691.168 kb on - strand, within DZA65_RS12550 at 2691.262 kb on + strand, within DZA65_RS12550 at 2691.374 kb on + strand, within DZA65_RS12550 at 2691.375 kb on - strand, within DZA65_RS12550 at 2691.441 kb on - strand at 2691.441 kb on - strand at 2691.441 kb on - strand at 2691.537 kb on + strand, within DZA65_RS12555 at 2691.537 kb on + strand, within DZA65_RS12555 at 2691.537 kb on + strand, within DZA65_RS12555 at 2691.538 kb on - strand, within DZA65_RS12555 at 2691.538 kb on - strand, within DZA65_RS12555 at 2691.538 kb on - strand, within DZA65_RS12555 at 2691.538 kb on - strand, within DZA65_RS12555 at 2691.538 kb on - strand, within DZA65_RS12555 at 2691.614 kb on + strand, within DZA65_RS12555 at 2691.615 kb on - strand, within DZA65_RS12555 at 2691.667 kb on - strand, within DZA65_RS12555 at 2691.810 kb on + strand, within DZA65_RS12555 at 2691.877 kb on + strand, within DZA65_RS12555 at 2691.877 kb on + strand, within DZA65_RS12555 at 2691.878 kb on - strand, within DZA65_RS12555 at 2691.882 kb on + strand, within DZA65_RS12555 at 2691.882 kb on + strand, within DZA65_RS12555 at 2691.882 kb on + strand, within DZA65_RS12555 at 2691.882 kb on + strand, within DZA65_RS12555 at 2691.882 kb on + strand, within DZA65_RS12555 at 2691.883 kb on - strand, within DZA65_RS12555 at 2691.883 kb on - strand, within DZA65_RS12555 at 2691.883 kb on - strand, within DZA65_RS12555 at 2691.883 kb on - strand, within DZA65_RS12555 at 2691.929 kb on + strand at 2691.930 kb on - strand at 2691.947 kb on + strand at 2691.947 kb on + strand at 2691.948 kb on - strand at 2691.965 kb on + strand at 2691.965 kb on + strand at 2691.983 kb on + strand at 2691.984 kb on - strand at 2692.102 kb on + strand at 2692.102 kb on + strand at 2692.102 kb on + strand at 2692.102 kb on + strand at 2692.103 kb on - strand at 2692.153 kb on + strand at 2692.154 kb on - strand at 2692.154 kb on - strand at 2692.154 kb on - strand at 2692.170 kb on - strand at 2692.187 kb on - strand at 2692.258 kb on - strand, within DZA65_RS12560 at 2692.260 kb on - strand, within DZA65_RS12560 at 2692.393 kb on + strand at 2692.393 kb on + strand at 2692.758 kb on + strand at 2692.827 kb on + strand at 2692.828 kb on - strand at 2692.829 kb on + strand at 2692.830 kb on - strand at 2692.889 kb on - strand at 2692.917 kb on - strand at 2692.974 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Upstate remove 2,690,929 - -2.3 2,690,953 - -1.1 2,690,983 + +2.7 2,690,984 - -0.8 2,691,074 + DZA65_RS12550 0.24 +2.1 2,691,074 + DZA65_RS12550 0.24 -1.4 2,691,074 + DZA65_RS12550 0.24 -0.1 2,691,074 + DZA65_RS12550 0.24 +0.5 2,691,075 - DZA65_RS12550 0.24 -0.1 2,691,077 - DZA65_RS12550 0.25 -0.2 2,691,168 - DZA65_RS12550 0.44 -0.2 2,691,262 + DZA65_RS12550 0.64 +0.9 2,691,374 + DZA65_RS12550 0.88 -0.3 2,691,375 - DZA65_RS12550 0.88 -0.4 2,691,441 - -1.5 2,691,441 - +0.5 2,691,441 - -0.5 2,691,537 + DZA65_RS12555 0.20 -0.1 2,691,537 + DZA65_RS12555 0.20 +0.3 2,691,537 + DZA65_RS12555 0.20 +1.0 2,691,538 - DZA65_RS12555 0.20 -0.3 2,691,538 - DZA65_RS12555 0.20 -1.3 2,691,538 - DZA65_RS12555 0.20 -0.2 2,691,538 - DZA65_RS12555 0.20 -1.8 2,691,538 - DZA65_RS12555 0.20 -2.8 2,691,614 + DZA65_RS12555 0.34 -1.1 2,691,615 - DZA65_RS12555 0.34 -0.5 2,691,667 - DZA65_RS12555 0.43 +0.5 2,691,810 + DZA65_RS12555 0.69 -1.4 2,691,877 + DZA65_RS12555 0.81 +2.7 2,691,877 + DZA65_RS12555 0.81 +0.3 2,691,878 - DZA65_RS12555 0.81 -0.9 2,691,882 + DZA65_RS12555 0.82 -1.0 2,691,882 + DZA65_RS12555 0.82 -1.8 2,691,882 + DZA65_RS12555 0.82 -1.8 2,691,882 + DZA65_RS12555 0.82 -0.7 2,691,882 + DZA65_RS12555 0.82 -0.7 2,691,883 - DZA65_RS12555 0.82 -1.3 2,691,883 - DZA65_RS12555 0.82 -0.1 2,691,883 - DZA65_RS12555 0.82 +1.8 2,691,883 - DZA65_RS12555 0.82 -2.4 2,691,929 + -0.3 2,691,930 - -0.8 2,691,947 + -0.3 2,691,947 + -2.5 2,691,948 - -0.5 2,691,965 + -0.6 2,691,965 + -0.1 2,691,983 + +0.1 2,691,984 - -1.1 2,692,102 + -2.8 2,692,102 + +0.1 2,692,102 + -1.4 2,692,102 + +1.0 2,692,103 - +0.1 2,692,153 + +2.8 2,692,154 - -0.8 2,692,154 - +0.8 2,692,154 - -1.1 2,692,170 - +0.7 2,692,187 - -0.1 2,692,258 - DZA65_RS12560 0.41 -1.5 2,692,260 - DZA65_RS12560 0.42 -0.1 2,692,393 + +0.7 2,692,393 + -0.5 2,692,758 + -0.5 2,692,827 + -0.6 2,692,828 - -0.3 2,692,829 + +0.1 2,692,830 - +2.1 2,692,889 - +2.6 2,692,917 - +0.3 2,692,974 - -0.5
Or see this region's nucleotide sequence