Experiment: Upstate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS14330 and DZA65_RS14335 are separated by 161 nucleotides DZA65_RS14335 and DZA65_RS14340 are separated by 415 nucleotides DZA65_RS14340 and DZA65_RS14345 are separated by 153 nucleotides DZA65_RS14345 and DZA65_RS14350 are separated by 16 nucleotides DZA65_RS14350 and DZA65_RS14355 overlap by 11 nucleotides
DZA65_RS14330: DZA65_RS14330 - helix-turn-helix transcriptional regulator, at 3,127,724 to 3,128,023
_RS14330
DZA65_RS14335: DZA65_RS14335 - transcriptional regulator, at 3,128,185 to 3,128,457
_RS14335
DZA65_RS14340: DZA65_RS14340 - hypothetical protein, at 3,128,873 to 3,129,787
_RS14340
DZA65_RS14345: DZA65_RS14345 - AlpA family transcriptional regulator, at 3,129,941 to 3,130,135
_RS14345
DZA65_RS14350: DZA65_RS14350 - DUF2857 domain-containing protein, at 3,130,152 to 3,130,736
_RS14350
DZA65_RS14355: DZA65_RS14355 - TraR/DksA family transcriptional regulator, at 3,130,726 to 3,130,935
_RS14355
Position (kb)
3128
3129
3130 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3127.899 kb on + strand, within DZA65_RS14330 at 3127.968 kb on - strand, within DZA65_RS14330 at 3127.988 kb on + strand, within DZA65_RS14330 at 3128.027 kb on - strand at 3128.027 kb on - strand at 3128.030 kb on - strand at 3128.030 kb on - strand at 3128.038 kb on + strand at 3128.038 kb on + strand at 3128.038 kb on + strand at 3128.038 kb on + strand at 3128.038 kb on + strand at 3128.039 kb on - strand at 3128.039 kb on - strand at 3128.039 kb on - strand at 3128.039 kb on - strand at 3128.039 kb on - strand at 3128.039 kb on - strand at 3128.184 kb on + strand at 3128.184 kb on + strand at 3128.184 kb on + strand at 3128.184 kb on + strand at 3128.184 kb on + strand at 3128.185 kb on - strand at 3128.185 kb on - strand at 3128.267 kb on + strand, within DZA65_RS14335 at 3128.268 kb on - strand, within DZA65_RS14335 at 3128.268 kb on - strand, within DZA65_RS14335 at 3128.295 kb on + strand, within DZA65_RS14335 at 3128.295 kb on + strand, within DZA65_RS14335 at 3128.295 kb on + strand, within DZA65_RS14335 at 3128.296 kb on - strand, within DZA65_RS14335 at 3128.351 kb on + strand, within DZA65_RS14335 at 3128.352 kb on - strand, within DZA65_RS14335 at 3128.494 kb on + strand at 3128.495 kb on - strand at 3128.548 kb on - strand at 3128.551 kb on + strand at 3128.551 kb on + strand at 3128.568 kb on + strand at 3128.569 kb on - strand at 3128.569 kb on - strand at 3128.584 kb on + strand at 3128.585 kb on - strand at 3128.603 kb on + strand at 3128.603 kb on + strand at 3128.631 kb on + strand at 3128.632 kb on - strand at 3128.674 kb on - strand at 3128.677 kb on + strand at 3128.677 kb on + strand at 3128.677 kb on + strand at 3128.677 kb on + strand at 3128.678 kb on - strand at 3128.678 kb on - strand at 3128.678 kb on - strand at 3128.678 kb on - strand at 3128.678 kb on - strand at 3128.776 kb on - strand at 3128.803 kb on - strand at 3128.803 kb on - strand at 3128.803 kb on - strand at 3128.859 kb on - strand at 3128.893 kb on - strand at 3128.927 kb on - strand at 3128.943 kb on + strand at 3128.943 kb on + strand at 3128.962 kb on - strand at 3128.993 kb on + strand, within DZA65_RS14340 at 3129.054 kb on + strand, within DZA65_RS14340 at 3129.128 kb on - strand, within DZA65_RS14340 at 3129.128 kb on - strand, within DZA65_RS14340 at 3129.189 kb on - strand, within DZA65_RS14340 at 3129.239 kb on + strand, within DZA65_RS14340 at 3129.258 kb on + strand, within DZA65_RS14340 at 3129.314 kb on + strand, within DZA65_RS14340 at 3129.315 kb on - strand, within DZA65_RS14340 at 3129.410 kb on - strand, within DZA65_RS14340 at 3129.427 kb on + strand, within DZA65_RS14340 at 3129.428 kb on - strand, within DZA65_RS14340 at 3129.466 kb on + strand, within DZA65_RS14340 at 3129.472 kb on + strand, within DZA65_RS14340 at 3129.595 kb on + strand, within DZA65_RS14340 at 3129.596 kb on - strand, within DZA65_RS14340 at 3129.596 kb on - strand, within DZA65_RS14340 at 3129.649 kb on + strand, within DZA65_RS14340 at 3129.650 kb on - strand, within DZA65_RS14340 at 3129.674 kb on + strand, within DZA65_RS14340 at 3129.677 kb on - strand, within DZA65_RS14340 at 3129.937 kb on - strand at 3129.941 kb on - strand at 3130.011 kb on - strand, within DZA65_RS14345 at 3130.011 kb on - strand, within DZA65_RS14345 at 3130.017 kb on - strand, within DZA65_RS14345 at 3130.017 kb on - strand, within DZA65_RS14345 at 3130.017 kb on - strand, within DZA65_RS14345 at 3130.017 kb on - strand, within DZA65_RS14345 at 3130.151 kb on + strand at 3130.151 kb on + strand at 3130.152 kb on - strand at 3130.152 kb on - strand at 3130.152 kb on - strand at 3130.235 kb on - strand, within DZA65_RS14350 at 3130.273 kb on + strand, within DZA65_RS14350 at 3130.320 kb on - strand, within DZA65_RS14350 at 3130.400 kb on + strand, within DZA65_RS14350 at 3130.427 kb on + strand, within DZA65_RS14350 at 3130.427 kb on + strand, within DZA65_RS14350 at 3130.427 kb on + strand, within DZA65_RS14350 at 3130.427 kb on + strand, within DZA65_RS14350 at 3130.428 kb on - strand, within DZA65_RS14350 at 3130.428 kb on - strand, within DZA65_RS14350 at 3130.428 kb on - strand, within DZA65_RS14350 at 3130.524 kb on - strand, within DZA65_RS14350 at 3130.592 kb on + strand, within DZA65_RS14350 at 3130.592 kb on + strand, within DZA65_RS14350 at 3130.683 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Upstate remove 3,127,899 + DZA65_RS14330 0.58 +0.9 3,127,968 - DZA65_RS14330 0.81 +1.7 3,127,988 + DZA65_RS14330 0.88 +2.3 3,128,027 - -1.7 3,128,027 - +0.4 3,128,030 - -1.1 3,128,030 - -0.1 3,128,038 + +0.3 3,128,038 + +0.0 3,128,038 + +0.5 3,128,038 + +0.5 3,128,038 + -0.7 3,128,039 - -0.2 3,128,039 - +1.6 3,128,039 - +0.7 3,128,039 - -0.9 3,128,039 - +0.3 3,128,039 - +1.0 3,128,184 + -0.1 3,128,184 + +1.0 3,128,184 + -0.8 3,128,184 + +1.5 3,128,184 + -1.3 3,128,185 - -0.1 3,128,185 - +1.1 3,128,267 + DZA65_RS14335 0.30 -0.7 3,128,268 - DZA65_RS14335 0.30 +1.2 3,128,268 - DZA65_RS14335 0.30 +1.0 3,128,295 + DZA65_RS14335 0.40 -0.3 3,128,295 + DZA65_RS14335 0.40 -1.0 3,128,295 + DZA65_RS14335 0.40 -0.2 3,128,296 - DZA65_RS14335 0.41 +0.7 3,128,351 + DZA65_RS14335 0.61 +1.3 3,128,352 - DZA65_RS14335 0.61 +0.7 3,128,494 + -0.5 3,128,495 - -2.8 3,128,548 - +0.8 3,128,551 + +3.2 3,128,551 + -0.4 3,128,568 + +0.4 3,128,569 - +0.7 3,128,569 - -0.5 3,128,584 + -0.2 3,128,585 - -0.1 3,128,603 + -1.7 3,128,603 + +0.3 3,128,631 + -0.9 3,128,632 - -1.7 3,128,674 - -0.5 3,128,677 + -0.5 3,128,677 + -0.1 3,128,677 + -1.3 3,128,677 + -0.2 3,128,678 - +0.2 3,128,678 - +0.9 3,128,678 - +1.6 3,128,678 - -0.5 3,128,678 - +0.5 3,128,776 - +0.4 3,128,803 - +1.1 3,128,803 - +0.3 3,128,803 - -0.0 3,128,859 - -0.5 3,128,893 - -1.3 3,128,927 - +0.7 3,128,943 + +2.6 3,128,943 + +1.1 3,128,962 - -0.1 3,128,993 + DZA65_RS14340 0.13 +0.9 3,129,054 + DZA65_RS14340 0.20 -0.1 3,129,128 - DZA65_RS14340 0.28 +3.7 3,129,128 - DZA65_RS14340 0.28 +0.0 3,129,189 - DZA65_RS14340 0.35 -1.1 3,129,239 + DZA65_RS14340 0.40 +0.5 3,129,258 + DZA65_RS14340 0.42 -0.2 3,129,314 + DZA65_RS14340 0.48 +0.4 3,129,315 - DZA65_RS14340 0.48 +1.1 3,129,410 - DZA65_RS14340 0.59 -0.7 3,129,427 + DZA65_RS14340 0.61 -1.0 3,129,428 - DZA65_RS14340 0.61 +1.0 3,129,466 + DZA65_RS14340 0.65 +0.7 3,129,472 + DZA65_RS14340 0.65 -1.2 3,129,595 + DZA65_RS14340 0.79 -1.5 3,129,596 - DZA65_RS14340 0.79 -0.3 3,129,596 - DZA65_RS14340 0.79 +0.3 3,129,649 + DZA65_RS14340 0.85 +1.3 3,129,650 - DZA65_RS14340 0.85 +0.4 3,129,674 + DZA65_RS14340 0.88 -1.1 3,129,677 - DZA65_RS14340 0.88 +0.7 3,129,937 - -0.1 3,129,941 - -0.8 3,130,011 - DZA65_RS14345 0.36 -1.3 3,130,011 - DZA65_RS14345 0.36 -1.3 3,130,017 - DZA65_RS14345 0.39 +1.5 3,130,017 - DZA65_RS14345 0.39 +1.3 3,130,017 - DZA65_RS14345 0.39 -0.1 3,130,017 - DZA65_RS14345 0.39 -1.3 3,130,151 + -0.4 3,130,151 + -0.4 3,130,152 - +0.3 3,130,152 - +0.2 3,130,152 - -0.0 3,130,235 - DZA65_RS14350 0.14 -0.2 3,130,273 + DZA65_RS14350 0.21 +0.1 3,130,320 - DZA65_RS14350 0.29 -0.4 3,130,400 + DZA65_RS14350 0.42 +1.1 3,130,427 + DZA65_RS14350 0.47 -2.8 3,130,427 + DZA65_RS14350 0.47 -1.5 3,130,427 + DZA65_RS14350 0.47 -2.3 3,130,427 + DZA65_RS14350 0.47 +0.4 3,130,428 - DZA65_RS14350 0.47 +0.5 3,130,428 - DZA65_RS14350 0.47 -0.6 3,130,428 - DZA65_RS14350 0.47 +0.7 3,130,524 - DZA65_RS14350 0.64 +1.3 3,130,592 + DZA65_RS14350 0.75 -2.0 3,130,592 + DZA65_RS14350 0.75 -0.7 3,130,683 - +0.2
Or see this region's nucleotide sequence