Experiment: Upstate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS02240 and DZA65_RS02245 are separated by 344 nucleotides DZA65_RS02245 and DZA65_RS02250 are separated by 2 nucleotides DZA65_RS02250 and DZA65_RS02255 are separated by 13 nucleotides
DZA65_RS02240: DZA65_RS02240 - DeoR/GlpR transcriptional regulator, at 486,625 to 487,416
_RS02240
DZA65_RS02245: DZA65_RS02245 - transketolase, at 487,761 to 488,564
_RS02245
DZA65_RS02250: DZA65_RS02250 - transketolase, at 488,567 to 489,487
_RS02250
DZA65_RS02255: DZA65_RS02255 - D-threitol dehydrogenase, at 489,501 to 490,304
_RS02255
Position (kb) 487
488
489 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 486.782 kb on + strand, within DZA65_RS02240 at 486.782 kb on + strand, within DZA65_RS02240 at 486.782 kb on + strand, within DZA65_RS02240 at 486.783 kb on - strand, within DZA65_RS02240 at 486.783 kb on - strand, within DZA65_RS02240 at 486.783 kb on - strand, within DZA65_RS02240 at 487.009 kb on - strand, within DZA65_RS02240 at 487.062 kb on + strand, within DZA65_RS02240 at 487.062 kb on + strand, within DZA65_RS02240 at 487.062 kb on + strand, within DZA65_RS02240 at 487.063 kb on - strand, within DZA65_RS02240 at 487.235 kb on + strand, within DZA65_RS02240 at 487.236 kb on - strand, within DZA65_RS02240 at 487.239 kb on - strand, within DZA65_RS02240 at 487.251 kb on - strand, within DZA65_RS02240 at 487.251 kb on - strand, within DZA65_RS02240 at 487.251 kb on - strand, within DZA65_RS02240 at 487.251 kb on - strand, within DZA65_RS02240 at 487.386 kb on + strand at 487.633 kb on + strand at 487.634 kb on - strand at 487.634 kb on - strand at 487.657 kb on - strand at 487.757 kb on - strand at 487.768 kb on + strand at 487.768 kb on + strand at 487.768 kb on + strand at 487.769 kb on - strand at 487.769 kb on - strand at 487.769 kb on - strand at 487.806 kb on + strand at 487.807 kb on - strand at 487.807 kb on - strand at 487.807 kb on - strand at 487.807 kb on - strand at 487.875 kb on - strand, within DZA65_RS02245 at 487.875 kb on - strand, within DZA65_RS02245 at 487.875 kb on - strand, within DZA65_RS02245 at 487.875 kb on - strand, within DZA65_RS02245 at 488.117 kb on - strand, within DZA65_RS02245 at 488.117 kb on - strand, within DZA65_RS02245 at 488.495 kb on - strand at 488.577 kb on + strand at 488.578 kb on - strand at 488.578 kb on - strand at 488.578 kb on - strand at 488.578 kb on - strand at 488.578 kb on - strand at 488.627 kb on - strand at 488.627 kb on - strand at 488.843 kb on + strand, within DZA65_RS02250 at 488.844 kb on - strand, within DZA65_RS02250 at 488.844 kb on - strand, within DZA65_RS02250 at 488.844 kb on - strand, within DZA65_RS02250 at 488.845 kb on + strand, within DZA65_RS02250 at 488.845 kb on + strand, within DZA65_RS02250 at 488.846 kb on - strand, within DZA65_RS02250 at 488.846 kb on - strand, within DZA65_RS02250 at 489.035 kb on + strand, within DZA65_RS02250 at 489.035 kb on + strand, within DZA65_RS02250 at 489.035 kb on + strand, within DZA65_RS02250 at 489.035 kb on + strand, within DZA65_RS02250 at 489.035 kb on + strand, within DZA65_RS02250 at 489.035 kb on + strand, within DZA65_RS02250 at 489.035 kb on + strand, within DZA65_RS02250 at 489.035 kb on + strand, within DZA65_RS02250 at 489.035 kb on + strand at 489.035 kb on + strand, within DZA65_RS02250 at 489.036 kb on - strand, within DZA65_RS02250 at 489.036 kb on - strand, within DZA65_RS02250 at 489.036 kb on - strand, within DZA65_RS02250 at 489.036 kb on - strand, within DZA65_RS02250 at 489.036 kb on - strand, within DZA65_RS02250 at 489.036 kb on - strand, within DZA65_RS02250 at 489.036 kb on - strand, within DZA65_RS02250 at 489.036 kb on - strand, within DZA65_RS02250 at 489.036 kb on - strand, within DZA65_RS02250 at 489.036 kb on - strand, within DZA65_RS02250 at 489.036 kb on - strand, within DZA65_RS02250 at 489.036 kb on - strand, within DZA65_RS02250 at 489.036 kb on - strand, within DZA65_RS02250 at 489.036 kb on - strand, within DZA65_RS02250 at 489.036 kb on - strand, within DZA65_RS02250 at 489.044 kb on - strand at 489.248 kb on - strand, within DZA65_RS02250 at 489.248 kb on - strand, within DZA65_RS02250 at 489.263 kb on + strand, within DZA65_RS02250 at 489.263 kb on + strand, within DZA65_RS02250 at 489.263 kb on + strand, within DZA65_RS02250 at 489.264 kb on - strand, within DZA65_RS02250 at 489.264 kb on - strand, within DZA65_RS02250 at 489.264 kb on - strand, within DZA65_RS02250 at 489.264 kb on - strand, within DZA65_RS02250 at 489.264 kb on - strand, within DZA65_RS02250 at 489.288 kb on + strand, within DZA65_RS02250 at 489.288 kb on + strand, within DZA65_RS02250 at 489.288 kb on + strand at 489.288 kb on + strand, within DZA65_RS02250 at 489.288 kb on + strand, within DZA65_RS02250 at 489.288 kb on + strand, within DZA65_RS02250 at 489.289 kb on - strand, within DZA65_RS02250 at 489.289 kb on - strand at 489.289 kb on - strand, within DZA65_RS02250 at 489.289 kb on - strand, within DZA65_RS02250 at 489.289 kb on - strand, within DZA65_RS02250 at 489.289 kb on - strand, within DZA65_RS02250 at 489.289 kb on - strand, within DZA65_RS02250 at 489.331 kb on + strand, within DZA65_RS02250 at 489.331 kb on + strand, within DZA65_RS02250 at 489.332 kb on - strand, within DZA65_RS02250 at 489.332 kb on - strand, within DZA65_RS02250 at 489.386 kb on - strand, within DZA65_RS02250 at 489.452 kb on - strand at 489.452 kb on - strand at 489.519 kb on + strand at 489.519 kb on + strand at 489.519 kb on + strand at 489.520 kb on - strand at 489.520 kb on - strand at 489.528 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Upstate remove 486,782 + DZA65_RS02240 0.20 -0.4 486,782 + DZA65_RS02240 0.20 +0.3 486,782 + DZA65_RS02240 0.20 +1.5 486,783 - DZA65_RS02240 0.20 +0.5 486,783 - DZA65_RS02240 0.20 +0.7 486,783 - DZA65_RS02240 0.20 -0.3 487,009 - DZA65_RS02240 0.48 -0.8 487,062 + DZA65_RS02240 0.55 +0.1 487,062 + DZA65_RS02240 0.55 -0.3 487,062 + DZA65_RS02240 0.55 -1.9 487,063 - DZA65_RS02240 0.55 -0.3 487,235 + DZA65_RS02240 0.77 +2.0 487,236 - DZA65_RS02240 0.77 -1.0 487,239 - DZA65_RS02240 0.78 +1.2 487,251 - DZA65_RS02240 0.79 +0.5 487,251 - DZA65_RS02240 0.79 +0.5 487,251 - DZA65_RS02240 0.79 +1.3 487,251 - DZA65_RS02240 0.79 -0.3 487,386 + -0.8 487,633 + -0.6 487,634 - +1.0 487,634 - -0.5 487,657 - -1.6 487,757 - -1.5 487,768 + -1.7 487,768 + +0.7 487,768 + -0.3 487,769 - +1.1 487,769 - +2.4 487,769 - -2.1 487,806 + +2.2 487,807 - -1.9 487,807 - -0.4 487,807 - -0.5 487,807 - +0.5 487,875 - DZA65_RS02245 0.14 -0.9 487,875 - DZA65_RS02245 0.14 -0.9 487,875 - DZA65_RS02245 0.14 -1.5 487,875 - DZA65_RS02245 0.14 -0.7 488,117 - DZA65_RS02245 0.44 -0.1 488,117 - DZA65_RS02245 0.44 -1.2 488,495 - -2.1 488,577 + -1.6 488,578 - +1.9 488,578 - -1.8 488,578 - +1.2 488,578 - -1.1 488,578 - +0.1 488,627 - -0.3 488,627 - -0.1 488,843 + DZA65_RS02250 0.30 -1.1 488,844 - DZA65_RS02250 0.30 +0.1 488,844 - DZA65_RS02250 0.30 +0.5 488,844 - DZA65_RS02250 0.30 +0.1 488,845 + DZA65_RS02250 0.30 +0.4 488,845 + DZA65_RS02250 0.30 -1.3 488,846 - DZA65_RS02250 0.30 +2.2 488,846 - DZA65_RS02250 0.30 -1.1 489,035 + DZA65_RS02250 0.51 +0.7 489,035 + DZA65_RS02250 0.51 -0.5 489,035 + DZA65_RS02250 0.51 -0.9 489,035 + DZA65_RS02250 0.51 -0.6 489,035 + DZA65_RS02250 0.51 -0.5 489,035 + DZA65_RS02250 0.51 +1.4 489,035 + DZA65_RS02250 0.51 +0.1 489,035 + DZA65_RS02250 0.51 +0.1 489,035 + -0.3 489,035 + DZA65_RS02250 0.51 -2.1 489,036 - DZA65_RS02250 0.51 +0.5 489,036 - DZA65_RS02250 0.51 +0.5 489,036 - DZA65_RS02250 0.51 -0.2 489,036 - DZA65_RS02250 0.51 -1.6 489,036 - DZA65_RS02250 0.51 -0.6 489,036 - DZA65_RS02250 0.51 +0.4 489,036 - DZA65_RS02250 0.51 -1.9 489,036 - DZA65_RS02250 0.51 +0.0 489,036 - DZA65_RS02250 0.51 +1.2 489,036 - DZA65_RS02250 0.51 +0.0 489,036 - DZA65_RS02250 0.51 +0.2 489,036 - DZA65_RS02250 0.51 +0.1 489,036 - DZA65_RS02250 0.51 +2.0 489,036 - DZA65_RS02250 0.51 +0.1 489,036 - DZA65_RS02250 0.51 -0.9 489,044 - -0.1 489,248 - DZA65_RS02250 0.74 -0.8 489,248 - DZA65_RS02250 0.74 +1.7 489,263 + DZA65_RS02250 0.76 -1.9 489,263 + DZA65_RS02250 0.76 -0.9 489,263 + DZA65_RS02250 0.76 -2.1 489,264 - DZA65_RS02250 0.76 +0.2 489,264 - DZA65_RS02250 0.76 +0.1 489,264 - DZA65_RS02250 0.76 +0.4 489,264 - DZA65_RS02250 0.76 -0.2 489,264 - DZA65_RS02250 0.76 -0.4 489,288 + DZA65_RS02250 0.78 -4.0 489,288 + DZA65_RS02250 0.78 -0.3 489,288 + +1.1 489,288 + DZA65_RS02250 0.78 -0.9 489,288 + DZA65_RS02250 0.78 +1.7 489,288 + DZA65_RS02250 0.78 -1.2 489,289 - DZA65_RS02250 0.78 +0.6 489,289 - +0.7 489,289 - DZA65_RS02250 0.78 +0.7 489,289 - DZA65_RS02250 0.78 +0.6 489,289 - DZA65_RS02250 0.78 +0.5 489,289 - DZA65_RS02250 0.78 +0.7 489,289 - DZA65_RS02250 0.78 -0.9 489,331 + DZA65_RS02250 0.83 -0.3 489,331 + DZA65_RS02250 0.83 -0.3 489,332 - DZA65_RS02250 0.83 -1.1 489,332 - DZA65_RS02250 0.83 -0.8 489,386 - DZA65_RS02250 0.89 +0.4 489,452 - -0.5 489,452 - -0.4 489,519 + +0.1 489,519 + +0.3 489,519 + -0.2 489,520 - +0.3 489,520 - -0.5 489,528 - +0.1
Or see this region's nucleotide sequence