Experiment: Norland
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS11440 and DZA65_RS11445 are separated by 78 nucleotides DZA65_RS11445 and DZA65_RS11450 are separated by 58 nucleotides
DZA65_RS11440: DZA65_RS11440 - MFS transporter, at 2,434,133 to 2,435,566
_RS11440
DZA65_RS11445: DZA65_RS11445 - fructotransferase, at 2,435,645 to 2,437,000
_RS11445
DZA65_RS11450: DZA65_RS11450 - carbohydrate kinase, at 2,437,059 to 2,438,027
_RS11450
Position (kb)
2435
2436
2437
2438 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 2434.792 kb on + strand, within DZA65_RS11440 at 2434.810 kb on - strand, within DZA65_RS11440 at 2434.829 kb on - strand, within DZA65_RS11440 at 2434.861 kb on + strand, within DZA65_RS11440 at 2434.862 kb on - strand, within DZA65_RS11440 at 2434.864 kb on + strand, within DZA65_RS11440 at 2434.865 kb on - strand, within DZA65_RS11440 at 2434.865 kb on - strand, within DZA65_RS11440 at 2434.917 kb on - strand, within DZA65_RS11440 at 2434.920 kb on - strand, within DZA65_RS11440 at 2434.928 kb on + strand, within DZA65_RS11440 at 2434.928 kb on + strand, within DZA65_RS11440 at 2434.928 kb on + strand, within DZA65_RS11440 at 2434.956 kb on + strand, within DZA65_RS11440 at 2434.966 kb on + strand, within DZA65_RS11440 at 2434.990 kb on + strand, within DZA65_RS11440 at 2434.991 kb on - strand at 2435.002 kb on + strand, within DZA65_RS11440 at 2435.002 kb on + strand, within DZA65_RS11440 at 2435.007 kb on + strand, within DZA65_RS11440 at 2435.011 kb on + strand, within DZA65_RS11440 at 2435.011 kb on + strand, within DZA65_RS11440 at 2435.011 kb on + strand, within DZA65_RS11440 at 2435.012 kb on - strand, within DZA65_RS11440 at 2435.137 kb on + strand, within DZA65_RS11440 at 2435.138 kb on - strand, within DZA65_RS11440 at 2435.161 kb on - strand, within DZA65_RS11440 at 2435.245 kb on + strand, within DZA65_RS11440 at 2435.327 kb on - strand, within DZA65_RS11440 at 2435.337 kb on + strand, within DZA65_RS11440 at 2435.338 kb on - strand, within DZA65_RS11440 at 2435.338 kb on - strand, within DZA65_RS11440 at 2435.415 kb on - strand, within DZA65_RS11440 at 2435.422 kb on + strand, within DZA65_RS11440 at 2435.422 kb on + strand, within DZA65_RS11440 at 2435.740 kb on + strand at 2435.799 kb on - strand, within DZA65_RS11445 at 2435.989 kb on - strand, within DZA65_RS11445 at 2436.115 kb on + strand, within DZA65_RS11445 at 2436.134 kb on + strand, within DZA65_RS11445 at 2436.155 kb on - strand, within DZA65_RS11445 at 2436.158 kb on + strand, within DZA65_RS11445 at 2436.159 kb on - strand, within DZA65_RS11445 at 2436.159 kb on - strand, within DZA65_RS11445 at 2436.196 kb on + strand, within DZA65_RS11445 at 2436.209 kb on + strand, within DZA65_RS11445 at 2436.213 kb on + strand, within DZA65_RS11445 at 2436.289 kb on + strand, within DZA65_RS11445 at 2436.326 kb on - strand, within DZA65_RS11445 at 2436.353 kb on - strand, within DZA65_RS11445 at 2436.394 kb on - strand, within DZA65_RS11445 at 2436.398 kb on - strand, within DZA65_RS11445 at 2436.499 kb on + strand, within DZA65_RS11445 at 2436.590 kb on + strand, within DZA65_RS11445 at 2436.590 kb on + strand, within DZA65_RS11445 at 2436.591 kb on - strand, within DZA65_RS11445 at 2436.591 kb on - strand, within DZA65_RS11445 at 2436.610 kb on + strand, within DZA65_RS11445 at 2436.611 kb on - strand, within DZA65_RS11445 at 2436.611 kb on - strand, within DZA65_RS11445 at 2436.611 kb on - strand, within DZA65_RS11445 at 2436.614 kb on + strand, within DZA65_RS11445 at 2436.614 kb on + strand, within DZA65_RS11445 at 2436.615 kb on - strand, within DZA65_RS11445 at 2436.626 kb on + strand, within DZA65_RS11445 at 2436.627 kb on - strand, within DZA65_RS11445 at 2436.629 kb on - strand at 2436.682 kb on + strand, within DZA65_RS11445 at 2436.690 kb on - strand, within DZA65_RS11445 at 2436.749 kb on + strand, within DZA65_RS11445 at 2436.750 kb on - strand, within DZA65_RS11445 at 2436.750 kb on - strand, within DZA65_RS11445 at 2436.750 kb on - strand, within DZA65_RS11445 at 2436.750 kb on - strand, within DZA65_RS11445 at 2436.750 kb on - strand, within DZA65_RS11445 at 2436.766 kb on + strand, within DZA65_RS11445 at 2436.767 kb on - strand, within DZA65_RS11445 at 2436.769 kb on + strand, within DZA65_RS11445 at 2436.769 kb on + strand, within DZA65_RS11445 at 2436.770 kb on - strand, within DZA65_RS11445 at 2436.770 kb on - strand, within DZA65_RS11445 at 2436.772 kb on + strand, within DZA65_RS11445 at 2436.772 kb on + strand, within DZA65_RS11445 at 2436.773 kb on - strand, within DZA65_RS11445 at 2436.773 kb on - strand, within DZA65_RS11445 at 2436.773 kb on - strand, within DZA65_RS11445 at 2436.786 kb on + strand, within DZA65_RS11445 at 2436.786 kb on + strand, within DZA65_RS11445 at 2436.834 kb on + strand, within DZA65_RS11445 at 2436.834 kb on + strand, within DZA65_RS11445 at 2436.843 kb on + strand, within DZA65_RS11445 at 2436.844 kb on - strand, within DZA65_RS11445 at 2436.848 kb on - strand, within DZA65_RS11445 at 2436.856 kb on + strand, within DZA65_RS11445 at 2436.856 kb on + strand, within DZA65_RS11445 at 2436.857 kb on - strand, within DZA65_RS11445 at 2436.857 kb on - strand, within DZA65_RS11445 at 2436.857 kb on - strand, within DZA65_RS11445 at 2436.876 kb on + strand at 2436.877 kb on - strand at 2436.932 kb on - strand at 2436.939 kb on + strand at 2436.939 kb on + strand at 2436.939 kb on + strand at 2436.940 kb on - strand at 2436.940 kb on - strand at 2436.952 kb on - strand at 2436.967 kb on + strand at 2436.968 kb on - strand at 2436.998 kb on + strand at 2436.998 kb on + strand at 2436.998 kb on + strand at 2436.998 kb on + strand at 2436.998 kb on + strand at 2436.999 kb on - strand at 2436.999 kb on - strand at 2436.999 kb on - strand at 2436.999 kb on - strand at 2436.999 kb on - strand at 2437.002 kb on + strand at 2437.002 kb on + strand at 2437.002 kb on + strand at 2437.002 kb on + strand at 2437.002 kb on + strand at 2437.003 kb on - strand at 2437.003 kb on - strand at 2437.005 kb on + strand at 2437.005 kb on + strand at 2437.007 kb on + strand at 2437.007 kb on + strand at 2437.007 kb on + strand at 2437.007 kb on + strand at 2437.008 kb on - strand at 2437.008 kb on - strand at 2437.018 kb on + strand at 2437.019 kb on - strand at 2437.046 kb on + strand at 2437.047 kb on - strand at 2437.089 kb on + strand at 2437.124 kb on + strand at 2437.146 kb on - strand at 2437.185 kb on + strand, within DZA65_RS11450 at 2437.185 kb on + strand, within DZA65_RS11450 at 2437.185 kb on + strand, within DZA65_RS11450 at 2437.186 kb on - strand, within DZA65_RS11450 at 2437.187 kb on + strand, within DZA65_RS11450 at 2437.187 kb on + strand, within DZA65_RS11450 at 2437.188 kb on - strand, within DZA65_RS11450 at 2437.188 kb on - strand, within DZA65_RS11450 at 2437.221 kb on + strand, within DZA65_RS11450 at 2437.221 kb on + strand, within DZA65_RS11450 at 2437.221 kb on + strand, within DZA65_RS11450 at 2437.221 kb on + strand, within DZA65_RS11450 at 2437.221 kb on + strand, within DZA65_RS11450 at 2437.222 kb on - strand, within DZA65_RS11450 at 2437.222 kb on - strand, within DZA65_RS11450 at 2437.222 kb on - strand, within DZA65_RS11450 at 2437.222 kb on - strand, within DZA65_RS11450 at 2437.269 kb on + strand, within DZA65_RS11450 at 2437.269 kb on + strand, within DZA65_RS11450 at 2437.269 kb on + strand, within DZA65_RS11450 at 2437.269 kb on + strand, within DZA65_RS11450 at 2437.269 kb on + strand, within DZA65_RS11450 at 2437.269 kb on + strand at 2437.269 kb on + strand, within DZA65_RS11450 at 2437.270 kb on - strand, within DZA65_RS11450 at 2437.270 kb on - strand, within DZA65_RS11450 at 2437.270 kb on - strand, within DZA65_RS11450 at 2437.270 kb on - strand, within DZA65_RS11450 at 2437.270 kb on - strand, within DZA65_RS11450 at 2437.276 kb on - strand, within DZA65_RS11450 at 2437.276 kb on - strand, within DZA65_RS11450 at 2437.276 kb on - strand, within DZA65_RS11450 at 2437.276 kb on - strand, within DZA65_RS11450 at 2437.346 kb on + strand, within DZA65_RS11450 at 2437.346 kb on + strand, within DZA65_RS11450 at 2437.375 kb on + strand, within DZA65_RS11450 at 2437.481 kb on + strand, within DZA65_RS11450 at 2437.579 kb on + strand, within DZA65_RS11450 at 2437.579 kb on + strand, within DZA65_RS11450 at 2437.579 kb on + strand, within DZA65_RS11450 at 2437.579 kb on + strand, within DZA65_RS11450 at 2437.580 kb on - strand, within DZA65_RS11450 at 2437.580 kb on - strand, within DZA65_RS11450 at 2437.580 kb on - strand, within DZA65_RS11450 at 2437.580 kb on - strand, within DZA65_RS11450 at 2437.580 kb on - strand, within DZA65_RS11450 at 2437.583 kb on + strand, within DZA65_RS11450 at 2437.583 kb on + strand, within DZA65_RS11450 at 2437.583 kb on + strand, within DZA65_RS11450 at 2437.583 kb on + strand, within DZA65_RS11450 at 2437.583 kb on + strand, within DZA65_RS11450 at 2437.583 kb on + strand, within DZA65_RS11450 at 2437.583 kb on + strand, within DZA65_RS11450 at 2437.583 kb on + strand, within DZA65_RS11450 at 2437.583 kb on + strand, within DZA65_RS11450 at 2437.583 kb on + strand, within DZA65_RS11450 at 2437.583 kb on + strand, within DZA65_RS11450 at 2437.583 kb on + strand, within DZA65_RS11450 at 2437.583 kb on + strand, within DZA65_RS11450 at 2437.584 kb on - strand, within DZA65_RS11450 at 2437.584 kb on - strand, within DZA65_RS11450 at 2437.584 kb on - strand, within DZA65_RS11450 at 2437.584 kb on - strand, within DZA65_RS11450 at 2437.584 kb on - strand, within DZA65_RS11450 at 2437.584 kb on - strand, within DZA65_RS11450 at 2437.584 kb on - strand, within DZA65_RS11450 at 2437.584 kb on - strand, within DZA65_RS11450 at 2437.613 kb on + strand, within DZA65_RS11450 at 2437.677 kb on + strand, within DZA65_RS11450 at 2437.726 kb on + strand, within DZA65_RS11450 at 2437.775 kb on + strand, within DZA65_RS11450 at 2437.945 kb on - strand at 2437.967 kb on + strand at 2437.968 kb on - strand at 2437.968 kb on - strand at 2437.968 kb on - strand at 2437.968 kb on - strand at 2437.968 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Norland remove 2,434,792 + DZA65_RS11440 0.46 -1.7 2,434,810 - DZA65_RS11440 0.47 -0.7 2,434,829 - DZA65_RS11440 0.49 -0.2 2,434,861 + DZA65_RS11440 0.51 +0.2 2,434,862 - DZA65_RS11440 0.51 -0.4 2,434,864 + DZA65_RS11440 0.51 -1.9 2,434,865 - DZA65_RS11440 0.51 +0.0 2,434,865 - DZA65_RS11440 0.51 -1.1 2,434,917 - DZA65_RS11440 0.55 -1.2 2,434,920 - DZA65_RS11440 0.55 -0.3 2,434,928 + DZA65_RS11440 0.55 +0.4 2,434,928 + DZA65_RS11440 0.55 +0.5 2,434,928 + DZA65_RS11440 0.55 +0.7 2,434,956 + DZA65_RS11440 0.57 +0.7 2,434,966 + DZA65_RS11440 0.58 -0.4 2,434,990 + DZA65_RS11440 0.60 -0.7 2,434,991 - +2.1 2,435,002 + DZA65_RS11440 0.61 -0.9 2,435,002 + DZA65_RS11440 0.61 +0.4 2,435,007 + DZA65_RS11440 0.61 +0.1 2,435,011 + DZA65_RS11440 0.61 +0.9 2,435,011 + DZA65_RS11440 0.61 +1.3 2,435,011 + DZA65_RS11440 0.61 -0.8 2,435,012 - DZA65_RS11440 0.61 +1.1 2,435,137 + DZA65_RS11440 0.70 -0.0 2,435,138 - DZA65_RS11440 0.70 +0.4 2,435,161 - DZA65_RS11440 0.72 -0.3 2,435,245 + DZA65_RS11440 0.78 +0.1 2,435,327 - DZA65_RS11440 0.83 -0.7 2,435,337 + DZA65_RS11440 0.84 -1.9 2,435,338 - DZA65_RS11440 0.84 +0.1 2,435,338 - DZA65_RS11440 0.84 -0.6 2,435,415 - DZA65_RS11440 0.89 +0.3 2,435,422 + DZA65_RS11440 0.90 +0.6 2,435,422 + DZA65_RS11440 0.90 +0.8 2,435,740 + +0.3 2,435,799 - DZA65_RS11445 0.11 -0.2 2,435,989 - DZA65_RS11445 0.25 +0.1 2,436,115 + DZA65_RS11445 0.35 -0.0 2,436,134 + DZA65_RS11445 0.36 +0.7 2,436,155 - DZA65_RS11445 0.38 +0.3 2,436,158 + DZA65_RS11445 0.38 -0.3 2,436,159 - DZA65_RS11445 0.38 -0.7 2,436,159 - DZA65_RS11445 0.38 +0.4 2,436,196 + DZA65_RS11445 0.41 -0.8 2,436,209 + DZA65_RS11445 0.42 +0.5 2,436,213 + DZA65_RS11445 0.42 +0.4 2,436,289 + DZA65_RS11445 0.47 -0.3 2,436,326 - DZA65_RS11445 0.50 -0.6 2,436,353 - DZA65_RS11445 0.52 +0.3 2,436,394 - DZA65_RS11445 0.55 +0.4 2,436,398 - DZA65_RS11445 0.56 +0.3 2,436,499 + DZA65_RS11445 0.63 -0.5 2,436,590 + DZA65_RS11445 0.70 -0.5 2,436,590 + DZA65_RS11445 0.70 -0.3 2,436,591 - DZA65_RS11445 0.70 -0.2 2,436,591 - DZA65_RS11445 0.70 -0.6 2,436,610 + DZA65_RS11445 0.71 +1.0 2,436,611 - DZA65_RS11445 0.71 -0.0 2,436,611 - DZA65_RS11445 0.71 +0.5 2,436,611 - DZA65_RS11445 0.71 -0.3 2,436,614 + DZA65_RS11445 0.71 -0.9 2,436,614 + DZA65_RS11445 0.71 +0.4 2,436,615 - DZA65_RS11445 0.72 -0.4 2,436,626 + DZA65_RS11445 0.72 +0.2 2,436,627 - DZA65_RS11445 0.72 +2.2 2,436,629 - +1.5 2,436,682 + DZA65_RS11445 0.76 +1.2 2,436,690 - DZA65_RS11445 0.77 +0.6 2,436,749 + DZA65_RS11445 0.81 +0.4 2,436,750 - DZA65_RS11445 0.81 +1.1 2,436,750 - DZA65_RS11445 0.81 -1.1 2,436,750 - DZA65_RS11445 0.81 +0.2 2,436,750 - DZA65_RS11445 0.81 +0.0 2,436,750 - DZA65_RS11445 0.81 -0.7 2,436,766 + DZA65_RS11445 0.83 -0.9 2,436,767 - DZA65_RS11445 0.83 -0.8 2,436,769 + DZA65_RS11445 0.83 +0.1 2,436,769 + DZA65_RS11445 0.83 +0.3 2,436,770 - DZA65_RS11445 0.83 -0.2 2,436,770 - DZA65_RS11445 0.83 -0.5 2,436,772 + DZA65_RS11445 0.83 +3.2 2,436,772 + DZA65_RS11445 0.83 +1.2 2,436,773 - DZA65_RS11445 0.83 -1.1 2,436,773 - DZA65_RS11445 0.83 +0.4 2,436,773 - DZA65_RS11445 0.83 -1.5 2,436,786 + DZA65_RS11445 0.84 +0.6 2,436,786 + DZA65_RS11445 0.84 +0.4 2,436,834 + DZA65_RS11445 0.88 +1.2 2,436,834 + DZA65_RS11445 0.88 +0.8 2,436,843 + DZA65_RS11445 0.88 +0.2 2,436,844 - DZA65_RS11445 0.88 -0.0 2,436,848 - DZA65_RS11445 0.89 -0.3 2,436,856 + DZA65_RS11445 0.89 -0.3 2,436,856 + DZA65_RS11445 0.89 -0.5 2,436,857 - DZA65_RS11445 0.89 +1.1 2,436,857 - DZA65_RS11445 0.89 +1.1 2,436,857 - DZA65_RS11445 0.89 +0.3 2,436,876 + +0.2 2,436,877 - +0.1 2,436,932 - -0.5 2,436,939 + +1.5 2,436,939 + +0.7 2,436,939 + -1.2 2,436,940 - +1.3 2,436,940 - +0.5 2,436,952 - +0.6 2,436,967 + -0.1 2,436,968 - +0.1 2,436,998 + -0.2 2,436,998 + +2.2 2,436,998 + -0.2 2,436,998 + +1.2 2,436,998 + +0.5 2,436,999 - -1.2 2,436,999 - -1.9 2,436,999 - -0.3 2,436,999 - -0.6 2,436,999 - -0.1 2,437,002 + +0.4 2,437,002 + +1.4 2,437,002 + +0.5 2,437,002 + +0.6 2,437,002 + -1.2 2,437,003 - -0.5 2,437,003 - -0.5 2,437,005 + +0.1 2,437,005 + -0.8 2,437,007 + +0.6 2,437,007 + -1.2 2,437,007 + -1.1 2,437,007 + +0.7 2,437,008 - +0.1 2,437,008 - +0.1 2,437,018 + +0.3 2,437,019 - +0.2 2,437,046 + +0.6 2,437,047 - -0.3 2,437,089 + -1.1 2,437,124 + +1.1 2,437,146 - -0.2 2,437,185 + DZA65_RS11450 0.13 -1.5 2,437,185 + DZA65_RS11450 0.13 -1.2 2,437,185 + DZA65_RS11450 0.13 -0.5 2,437,186 - DZA65_RS11450 0.13 -0.4 2,437,187 + DZA65_RS11450 0.13 +0.9 2,437,187 + DZA65_RS11450 0.13 -0.5 2,437,188 - DZA65_RS11450 0.13 +0.6 2,437,188 - DZA65_RS11450 0.13 +1.3 2,437,221 + DZA65_RS11450 0.17 +0.8 2,437,221 + DZA65_RS11450 0.17 -2.1 2,437,221 + DZA65_RS11450 0.17 +1.2 2,437,221 + DZA65_RS11450 0.17 +0.1 2,437,221 + DZA65_RS11450 0.17 +0.5 2,437,222 - DZA65_RS11450 0.17 -0.1 2,437,222 - DZA65_RS11450 0.17 -1.7 2,437,222 - DZA65_RS11450 0.17 +0.1 2,437,222 - DZA65_RS11450 0.17 -0.7 2,437,269 + DZA65_RS11450 0.22 -1.4 2,437,269 + DZA65_RS11450 0.22 +0.4 2,437,269 + DZA65_RS11450 0.22 -1.3 2,437,269 + DZA65_RS11450 0.22 +1.2 2,437,269 + DZA65_RS11450 0.22 -2.2 2,437,269 + +5.1 2,437,269 + DZA65_RS11450 0.22 +1.9 2,437,270 - DZA65_RS11450 0.22 -1.1 2,437,270 - DZA65_RS11450 0.22 +0.3 2,437,270 - DZA65_RS11450 0.22 -0.1 2,437,270 - DZA65_RS11450 0.22 +0.1 2,437,270 - DZA65_RS11450 0.22 -2.9 2,437,276 - DZA65_RS11450 0.22 +0.2 2,437,276 - DZA65_RS11450 0.22 -0.1 2,437,276 - DZA65_RS11450 0.22 -0.1 2,437,276 - DZA65_RS11450 0.22 +2.0 2,437,346 + DZA65_RS11450 0.30 +1.2 2,437,346 + DZA65_RS11450 0.30 +0.9 2,437,375 + DZA65_RS11450 0.33 -0.7 2,437,481 + DZA65_RS11450 0.44 -0.6 2,437,579 + DZA65_RS11450 0.54 -0.7 2,437,579 + DZA65_RS11450 0.54 -0.9 2,437,579 + DZA65_RS11450 0.54 +0.7 2,437,579 + DZA65_RS11450 0.54 -0.5 2,437,580 - DZA65_RS11450 0.54 -0.1 2,437,580 - DZA65_RS11450 0.54 -0.9 2,437,580 - DZA65_RS11450 0.54 +0.5 2,437,580 - DZA65_RS11450 0.54 +1.7 2,437,580 - DZA65_RS11450 0.54 -0.1 2,437,583 + DZA65_RS11450 0.54 -1.3 2,437,583 + DZA65_RS11450 0.54 -1.2 2,437,583 + DZA65_RS11450 0.54 +0.5 2,437,583 + DZA65_RS11450 0.54 +0.3 2,437,583 + DZA65_RS11450 0.54 -0.9 2,437,583 + DZA65_RS11450 0.54 +0.6 2,437,583 + DZA65_RS11450 0.54 -0.7 2,437,583 + DZA65_RS11450 0.54 -1.2 2,437,583 + DZA65_RS11450 0.54 -1.9 2,437,583 + DZA65_RS11450 0.54 -0.2 2,437,583 + DZA65_RS11450 0.54 -2.7 2,437,583 + DZA65_RS11450 0.54 +2.4 2,437,583 + DZA65_RS11450 0.54 +0.2 2,437,584 - DZA65_RS11450 0.54 -1.1 2,437,584 - DZA65_RS11450 0.54 +0.8 2,437,584 - DZA65_RS11450 0.54 -0.8 2,437,584 - DZA65_RS11450 0.54 -1.5 2,437,584 - DZA65_RS11450 0.54 +0.9 2,437,584 - DZA65_RS11450 0.54 +1.0 2,437,584 - DZA65_RS11450 0.54 -0.5 2,437,584 - DZA65_RS11450 0.54 +0.5 2,437,613 + DZA65_RS11450 0.57 +0.6 2,437,677 + DZA65_RS11450 0.64 -0.1 2,437,726 + DZA65_RS11450 0.69 +0.2 2,437,775 + DZA65_RS11450 0.74 +0.2 2,437,945 - +1.2 2,437,967 + -0.8 2,437,968 - +0.1 2,437,968 - -3.1 2,437,968 - -1.1 2,437,968 - -1.0 2,437,968 - -0.2
Or see this region's nucleotide sequence